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Article: The Effects of

Yurkov, Andrey P / Afonin, Alexey M / Kryukov, Alexey A / Gorbunova, Anastasia O / Kudryashova, Tatyana R / Kovalchuk, Anastasia I / Gorenkova, Anastasia I / Bogdanova, Ekaterina M / Kosulnikov, Yuri V / Laktionov, Yuri V / Kozhemyakov, Andrey P / Romanyuk, Daria A / Zhukov, Vladimir A / Puzanskiy, Roman K / Mikhailova, Yulia V / Yemelyanov, Vladislav V / Shishova, Maria F

Plants (Basel, Switzerland)

2023 Volume 12, Issue 20

Abstract: The study is aimed at revealing the effects ... ...

Abstract	The study is aimed at revealing the effects of
Language	English
Publishing date	2023-10-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants12203580
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The Role of

Yurkov, Andrey P / Puzanskiy, Roman K / Kryukov, Alexey A / Gorbunova, Anastasiia O / Kudriashova, Tatyana R / Jacobi, Lidija M / Kozhemyakov, Andrei P / Romanyuk, Daria A / Aronova, Ekaterina B / Avdeeva, Galina S / Yemelyanov, Vladislav V / Shavarda, Alexey L / Shishova, Maria F

Plants (Basel, Switzerland)

2022 Volume 11, Issue 18

Abstract: The nature of plant-fungi interaction at early stages of arbuscular mycorrhiza (AM) development is still a puzzling problem. To investigate the processes behind this interaction, we used ... ...

Abstract	The nature of plant-fungi interaction at early stages of arbuscular mycorrhiza (AM) development is still a puzzling problem. To investigate the processes behind this interaction, we used the
Language	English
Publishing date	2022-09-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11182338
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Article: Mycorrhiza-Induced Alterations in Metabolome of

Yurkov, Andrey P / Puzanskiy, Roman K / Avdeeva, Galina S / Jacobi, Lidija M / Gorbunova, Anastasia O / Kryukov, Alexey A / Kozhemyakov, Andrei P / Laktionov, Yuri V / Kosulnikov, Yuri V / Romanyuk, Daria A / Yemelyanov, Vladislav V / Shavarda, Alexey L / Kirpichnikova, Anastasia A / Smolikova, Galina N / Shishova, Maria F

Plants (Basel, Switzerland)

2021 Volume 10, Issue 11

Abstract: The present study is aimed at disclosing metabolic profile alterations in the leaves of ... ...

Abstract	The present study is aimed at disclosing metabolic profile alterations in the leaves of the
Language	English
Publishing date	2021-11-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants10112506
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Article ; Online: Binding of chloroaurate to polytyrosine-PEG micelles leads to an anti-Turkevich pattern of reduction.

Iakimov, Nikolai P / Romanyuk, Andrey V / Grozdova, Irina D / Dets, Elisabeth A / Alov, Nikolai V / Sharanov, Pavel Yu / Maksimov, Sergey V / Savilov, Serguei V / Abramchuk, Sergey S / Ksenofontov, Alexander L / Eremina, Elena A / Melik-Nubarov, Nikolay S

Soft matter

2021 Volume 17, Issue 10, Page(s) 2711–2724

Abstract: Here we report formation of gold nanoparticles (GNPs) in micelles of polytyrosine-PEG copolymers that combine the properties of a reducer and a stabilizer. The size and properties of the GNPs were tailored by the excess chloroaurate over the copolymer. ... ...

Abstract	Here we report formation of gold nanoparticles (GNPs) in micelles of polytyrosine-PEG copolymers that combine the properties of a reducer and a stabilizer. The size and properties of the GNPs were tailored by the excess chloroaurate over the copolymer. The latter quickly formed non-covalent complexes with HAuCl4 and then slowly reduced it to form GNPs. 3 Tyr residues are consumed by reduction of one mole of chloroaurate. The size of the GNPs was controlled by the [Tyr]/[Au(iii)] molar ratio. Small GNPs with D ≅ 8 nm were formed at [Tyr]/[Au(iii)] = 0.5-1.5. 90% of these small GNPs remained bound to the copolymer and could be stored in a lyophilized state. Such polypeptide-gold hybrid materials produced at [Tyr]/[Au(iii)] = 0.5 demonstrated high activity in the catalytic reduction of 4-nitrophenol by sodium borohydride. [Tyr]/[Au(iii)] = 5 led to the formation of large nanoplates (D ≅ 30-60 nm). Thus, in the polymer-based system the GNP size grew in line with the excess of the reducing agent in contrast to Turkevich synthesis of GNPs with citric acid, which also combines the functions of a stabilizer and a reducer. The difference results from the reduction of HAuCl4 in solution according to the Turkevich method and in the micelles of the amphiphilic polymer where the seed growth is limited by the amount of neighboring reducer.
Language	English
Publishing date	2021-02-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2191476-X
ISSN	1744-6848 ; 1744-683X
ISSN (online)	1744-6848
ISSN	1744-683X
DOI	10.1039/d0sm02259d
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Article ; Online: Peroxyoxalate Chemiluminescent Reaction as a Tool for Elimination of Tumour Cells Under Oxidative Stress.

Romanyuk, Andrey V / Grozdova, Irina D / Ezhov, Alexander A / Melik-Nubarov, Nickolay S

Scientific reports

2017 Volume 7, Issue 1, Page(s) 3410

Abstract: The overproduction of hydrogen peroxide is an inherent feature of some tumour cells and inflamed tissues. We took advantage of this peculiarity to eliminate cells using chemiluminescent peroxyoxalate reaction. We designed dispersions containing ... ...

Abstract	The overproduction of hydrogen peroxide is an inherent feature of some tumour cells and inflamed tissues. We took advantage of this peculiarity to eliminate cells using chemiluminescent peroxyoxalate reaction. We designed dispersions containing polyoxalate and tetramethylhematoporhyrin (TMHP) in dimethylphthalate droplets stabilized with Pluronic L64. The porphyrin plays the dual role. On the one hand, it serves as an activator of the peroxyoxalate reaction of polyoxalate with intracellular hydrogen peroxide and experiences excitation as a result of the reaction. The light emitted in the reaction in the model system without cells was used to optimize the dispersion's composition. On the other hand, TMHP acts as a photosensitizer (PS) causing cell damage. The formation of singlet oxygen led to cell elimination if the dispersions were used in combination with inducers of oxidative stress: hydrogen peroxide, paraquat, antitumour drug doxorubicin, or a nutritional additive menadione. The PS-induced cytotoxicity correlated with the level of intracellular ROS. The developed approach targeted to endogenous ROS is orthogonal to the classical chemotherapy and can be applied to increase its efficiency.
MeSH term(s)	Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Cell Death/drug effects ; Humans ; Luminescence ; MCF-7 Cells ; Oxalates/chemistry ; Oxalates/pharmacology ; Oxidative Stress ; Photochemotherapy/methods ; Photosensitizing Agents/chemistry ; Photosensitizing Agents/pharmacology ; Poloxamer/chemistry
Chemical Substances	Antineoplastic Agents ; Oxalates ; Photosensitizing Agents ; Poloxamer (106392-12-5) ; peroxyoxalate (5796-84-9) ; pluronic L64 (BIS92ZSF57)
Language	English
Publishing date	2017-06-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-017-03527-w
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Article ; Online: Peroxyoxalate Chemiluminescent Reaction as a Tool for Elimination of Tumour Cells Under Oxidative Stress

Andrey V. Romanyuk / Irina D. Grozdova / Alexander A. Ezhov / Nickolay S. Melik-Nubarov

Scientific Reports, Vol 7, Iss 1, Pp 1-

2017 Volume 13

Abstract: Abstract The overproduction of hydrogen peroxide is an inherent feature of some tumour cells and inflamed tissues. We took advantage of this peculiarity to eliminate cells using chemiluminescent peroxyoxalate reaction. We designed dispersions containing ... ...

Abstract	Abstract The overproduction of hydrogen peroxide is an inherent feature of some tumour cells and inflamed tissues. We took advantage of this peculiarity to eliminate cells using chemiluminescent peroxyoxalate reaction. We designed dispersions containing polyoxalate and tetramethylhematoporhyrin (TMHP) in dimethylphthalate droplets stabilized with Pluronic L64. The porphyrin plays the dual role. On the one hand, it serves as an activator of the peroxyoxalate reaction of polyoxalate with intracellular hydrogen peroxide and experiences excitation as a result of the reaction. The light emitted in the reaction in the model system without cells was used to optimize the dispersion’s composition. On the other hand, TMHP acts as a photosensitizer (PS) causing cell damage. The formation of singlet oxygen led to cell elimination if the dispersions were used in combination with inducers of oxidative stress: hydrogen peroxide, paraquat, antitumour drug doxorubicin, or a nutritional additive menadione. The PS-induced cytotoxicity correlated with the level of intracellular ROS. The developed approach targeted to endogenous ROS is orthogonal to the classical chemotherapy and can be applied to increase its efficiency.
Keywords	Medicine ; R ; Science ; Q
Subject code	500
Language	English
Publishing date	2017-06-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: The Role of Medicago lupulina Interaction with Rhizophagus irregularis in the Determination of Root Metabolome at Early Stages of AM Symbiosis

Yurkov, Andrey P. / Puzanskiy, Roman K. / Kryukov, Alexey A. / Gorbunova, Anastasiia O. / Kudriashova, Tatyana R. / Jacobi, Lidija M. / Kozhemyakov, Andrei P. / Romanyuk, Daria A. / Aronova, Ekaterina B. / Avdeeva, Galina S. / Yemelyanov, Vladislav V. / Shavarda, Alexey L. / Shishova, Maria F.

Plants. 2022 Sept. 07, v. 11, no. 18

2022

Abstract: The nature of plant–fungi interaction at early stages of arbuscular mycorrhiza (AM) development is still a puzzling problem. To investigate the processes behind this interaction, we used the Medicago lupulina MlS-1 line that forms high-efficient AM ... ...

Abstract	The nature of plant–fungi interaction at early stages of arbuscular mycorrhiza (AM) development is still a puzzling problem. To investigate the processes behind this interaction, we used the Medicago lupulina MlS-1 line that forms high-efficient AM symbiosis with Rhizophagus irregularis. AM fungus actively colonizes the root system of the host plant and contributes to the formation of effective AM as characterized by a high mycorrhizal growth response (MGR) in the host plant. The present study is aimed at distinguishing the alterations in the M. lupulina root metabolic profile as an indicative marker of effective symbiosis. We examined the root metabolome at the 14th and 24th day after sowing and inoculation (DAS) with low substrate phosphorus levels. A GS-MS analysis detected 316 metabolites. Results indicated that profiles of M. lupulina root metabolites differed from those in leaves previously detected. The roots contained fewer sugars and organic acids. Hence, compounds supporting the growth of mycorrhizal fungus (especially amino acids, specific lipids, and carbohydrates) accumulated, and their presence coincided with intensive development of AM structures. Mycorrhization determined the root metabolite profile to a greater extent than host plant development. The obtained data highlight the importance of active plant–fungi metabolic interaction at early stages of host plant development for the determination of symbiotic efficiency.
Keywords	Medicago lupulina ; Rhizophagus irregularis ; host plants ; metabolites ; metabolome ; mycorrhizal fungi ; phosphorus ; plant development ; root systems ; vesicular arbuscular mycorrhizae
Language	English
Dates of publication	2022-0907
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11182338
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Collection of analytes from microneedle patches.

Romanyuk, Andrey V / Zvezdin, Vasiliy N / Samant, Pradnya / Grenader, Mark I / Zemlyanova, Marina / Prausnitz, Mark R

Analytical chemistry

2014 Volume 86, Issue 21, Page(s) 10520–10523

Abstract: ... patches, the patches were mounted within the cap of microcentrifuge tubes or formed the top of V-bottom ...

Abstract	Clinical medicine and public health would benefit from simplified acquisition of biological samples from patients that can be easily obtained at point of care, in the field, and by patients themselves. Microneedle patches are designed to serve this need by collecting dermal interstitial fluid containing biomarkers without the dangers, pain, or expertise needed to collect blood. This study presents novel methods to collect biomarker analytes from microneedle patches for analysis by integration into conventional analytical laboratory microtubes and microplates. Microneedle patches were made out of cross-linked hydrogel composed of poly(methyl vinyl ether-alt-maleic acid) and poly(ethylene glycol) prepared by micromolding. Microneedle patches were shown to swell with water up to 50-fold in volume, depending on degree of polymer cross-linking, and to collect interstitial fluid from the skin of rats. To collect analytes from microneedle patches, the patches were mounted within the cap of microcentrifuge tubes or formed the top of V-bottom multiwell microplates, and fluid was collected in the bottom of the tubes under gentle centrifugation. In another method, microneedle patches were attached to form the bottom of multiwell microplates, thereby enabling in situ analysis. The simplicity of biological sample acquisition using microneedle patches coupled with the simplicity of analyte collection from microneedles patches integrated into conventional analytical equipment could broaden the reach of future screening, diagnosis, and monitoring of biomarkers in healthcare and environmental/workplace settings.
MeSH term(s)	Animals ; Equipment Design ; Extracellular Fluid/chemistry ; Hydrogels/chemistry ; Needles ; Polyethylene Glycols/chemistry ; Rats ; Skin/chemistry ; Specimen Handling/instrumentation
Chemical Substances	Hydrogels ; Polyethylene Glycols (30IQX730WE)
Language	English
Publishing date	2014-11-04
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/ac503823p
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Article ; Online: Mammalian amyloidogenic proteins promote prion nucleation in yeast.

Chandramowlishwaran, Pavithra / Sun, Meng / Casey, Kristin L / Romanyuk, Andrey V / Grizel, Anastasiya V / Sopova, Julia V / Rubel, Aleksandr A / Nussbaum-Krammer, Carmen / Vorberg, Ina M / Chernoff, Yury O

The Journal of biological chemistry

2018 Volume 293, Issue 9, Page(s) 3436–3450

Abstract: Fibrous cross-β aggregates (amyloids) and their transmissible forms (prions) cause diseases in mammals (including humans) and control heritable traits in yeast. Initial nucleation of a yeast prion by transiently overproduced prion-forming protein or its ( ...

Abstract	Fibrous cross-β aggregates (amyloids) and their transmissible forms (prions) cause diseases in mammals (including humans) and control heritable traits in yeast. Initial nucleation of a yeast prion by transiently overproduced prion-forming protein or its (typically, QN-rich) prion domain is efficient only in the presence of another aggregated (in most cases, QN-rich) protein. Here, we demonstrate that a fusion of the prion domain of yeast protein Sup35 to some non-QN-rich mammalian proteins, associated with amyloid diseases, promotes nucleation of Sup35 prions in the absence of pre-existing aggregates. In contrast, both a fusion of the Sup35 prion domain to a multimeric non-amyloidogenic protein and the expression of a mammalian amyloidogenic protein that is not fused to the Sup35 prion domain failed to promote prion nucleation, further indicating that physical linkage of a mammalian amyloidogenic protein to the prion domain of a yeast protein is required for the nucleation of a yeast prion. Biochemical and cytological approaches confirmed the nucleation of protein aggregates in the yeast cell. Sequence alterations antagonizing or enhancing amyloidogenicity of human amyloid-β (associated with Alzheimer's disease) and mouse prion protein (associated with prion diseases), respectively, antagonized or enhanced nucleation of a yeast prion by these proteins. The yeast-based prion nucleation assay, developed in our work, can be employed for mutational dissection of amyloidogenic proteins. We anticipate that it will aid in the identification of chemicals that influence initial amyloid nucleation and in searching for new amyloidogenic proteins in a variety of proteomes.
MeSH term(s)	Amyloid/metabolism ; Amyloid beta-Peptides/metabolism ; Humans ; Peptide Fragments/metabolism ; Peptide Termination Factors/chemistry ; Peptide Termination Factors/metabolism ; Protein Aggregates ; Protein Domains ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/metabolism
Chemical Substances	Amyloid ; Amyloid beta-Peptides ; Peptide Fragments ; Peptide Termination Factors ; Protein Aggregates ; SUP35 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; amyloid beta-protein (1-40) ; amyloid beta-protein (1-42)
Language	English
Publishing date	2018-01-12
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.M117.809004
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Article ; Online: Stable incorporation of GM-CSF into dissolvable microneedle patch improves skin vaccination against influenza.

Littauer, Elizabeth Q / Mills, Lisa K / Brock, Nicole / Esser, E Stein / Romanyuk, Andrey / Pulit-Penaloza, Joanna A / Vassilieva, Elena V / Beaver, Jacob T / Antao, Olivia / Krammer, Florian / Compans, Richard W / Prausnitz, Mark R / Skountzou, Ioanna

Journal of controlled release : official journal of the Controlled Release Society

2018 Volume 276, Page(s) 1–16

Abstract: The widely used influenza subunit vaccine would benefit from increased protection rates in vulnerable populations. Skin immunization by microneedle (MN) patch can increase vaccine immunogenicity, as well as increase vaccination coverage due to simplified ...

Abstract	The widely used influenza subunit vaccine would benefit from increased protection rates in vulnerable populations. Skin immunization by microneedle (MN) patch can increase vaccine immunogenicity, as well as increase vaccination coverage due to simplified administration. To further increase immunogenicity, we used granulocyte-macrophage colony stimulating factor (GM-CSF), an immunomodulatory cytokine already approved for skin cancer therapy and cancer support treatment. GM-CSF has been shown to be upregulated in skin following MN insertion. The GM-CSF-adjuvanted vaccine induced robust and long-lived antibody responses cross-reactive to homosubtypic and heterosubtypic influenza viruses. Addition of GM-CSF resulted in increased memory B cell persistence relative to groups given influenza vaccine alone and led to rapid lung viral clearance following lethal infection with homologous virus in the mouse model. Here we demonstrate that successful incorporation of the thermolabile cytokine GM-CSF into MN resulted in improved vaccine-induced protective immunity holding promise as a novel approach to improved influenza vaccination. To our knowledge, this is the first successful incorporation of a cytokine adjuvant into dissolvable MNs, thus advancing and diversifying the rapidly developing field of MN vaccination technology.
MeSH term(s)	Administration, Cutaneous ; Animals ; Dogs ; Female ; Granulocyte-Macrophage Colony-Stimulating Factor/administration & dosage ; Influenza A Virus, H1N1 Subtype ; Influenza A Virus, H3N2 Subtype ; Influenza Vaccines/administration & dosage ; Injections, Intradermal ; Madin Darby Canine Kidney Cells ; Mice, Inbred BALB C ; Microinjections ; Needles ; Orthomyxoviridae Infections/prevention & control ; Transdermal Patch ; Vaccination/methods
Chemical Substances	Influenza Vaccines ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1)
Language	English
Publishing date	2018-02-26
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	632533-6
ISSN	1873-4995 ; 0168-3659
ISSN (online)	1873-4995
ISSN	0168-3659
DOI	10.1016/j.jconrel.2018.02.033
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