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Article ; Online: Gelatin-based cell culture device for construction and X-ray irradiation of a three-dimensional oral cancer model.

Bessho, Tomoka / Takagi, Tomoko / Igawa, Kazuyo / Sato, Kae

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

2023 Volume 39, Issue 6, Page(s) 771–778

Abstract: Bioassays using three-dimensional (3D) tissue models offer several advantages over 2D culture assays because they can reproduce the structure and function of native tissues. In this study, we used our newly designed gelatin device to generate a miniature ...

Abstract	Bioassays using three-dimensional (3D) tissue models offer several advantages over 2D culture assays because they can reproduce the structure and function of native tissues. In this study, we used our newly designed gelatin device to generate a miniature 3D model of human oral squamous cell carcinoma with stroma and blood vessels. To enable air-liquid interface culture, we conceived a new device structure in which three wells were lined up and separated by a dividing thread; the wells could be connected by removing the dividing thread. Cells were seeded in the center well with the dividing thread to form a multilayer, followed by the supply of media from the side wells after thread removal. Human oral squamous cell carcinoma (HSC-4) cells, human umbilical vein endothelial cells (HUVECs), and normal human dermal fibroblasts (NHDFs) were successfully cocultured, resulting in structures that mimicked 3D-cancer tissues. This 3D-cancer model was subjected to an X-ray sensitivity assay, followed by the evaluation of DNA damage using confocal microscopy and section-scanning electron microscopy.
MeSH term(s)	Humans ; Gelatin/chemistry ; X-Rays ; Carcinoma, Squamous Cell ; Squamous Cell Carcinoma of Head and Neck ; Mouth Neoplasms ; Cell Culture Techniques ; Human Umbilical Vein Endothelial Cells ; Head and Neck Neoplasms
Chemical Substances	Gelatin (9000-70-8)
Language	English
Publishing date	2023-02-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1483376-1
ISSN	1348-2246 ; 1348-2246
ISSN (online)	1348-2246
ISSN	1348-2246
DOI	10.1007/s44211-023-00308-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Nucleic acid-extracted torula yeast from the paper industry as a protein feed for ruminants: A comparison with soybean meal.

Liu, Chunyan / Asano, Sanae / Sato, Saeko / Murai, Kae / Yabe, Nanami / Kajikawa, Hiroshi

Animal science journal = Nihon chikusan Gakkaiho

2024 Volume 95, Issue 1, Page(s) e13948

Abstract: We compared nucleic acid-extracted torula yeast (NTY) with soybean meal (SBM) to evaluate NTY as a potential protein feed for ruminants in a metabolic trial using four castrated male goats. NTY was replaced isonitrogenously with SBM at a 25% crude ... ...

Abstract	We compared nucleic acid-extracted torula yeast (NTY) with soybean meal (SBM) to evaluate NTY as a potential protein feed for ruminants in a metabolic trial using four castrated male goats. NTY was replaced isonitrogenously with SBM at a 25% crude protein (CP) level on a dry matter (DM) basis. NTY has 55% CP and 74% total digestive nutrients on DM. Absorbed N was lower on the NTY diet, but since the urinary N excretion was lower on the NTY diet, no significant between-diet difference in retained N was observed. The efficiency of N utilization (retained N/absorbed N) was significantly higher on the NTY diet. The Lys and Met contents (presumed limiting amino acids for dairy cattle) were higher in NTY than SBM, which may be why N utilization efficiency was higher for the NTY diet. Ruminal ammonia-N and blood serum N were lower on the NTY diet, suggesting that NTY has more rumen undegradable protein than SBM. There was no significant between-diet difference in the visceral disorder indicators or antioxidant activities. Our results indicate that NTY is a safe protein feed with a high CP ratio and high-quality amino acid profile for ruminants that is equivalent to SBM.
MeSH term(s)	Cattle ; Male ; Animals ; Saccharomyces cerevisiae ; Cryptococcus ; Animal Feed/analysis ; Flour ; Dietary Proteins/metabolism ; Rumen/metabolism ; Nutrients ; Glycine max ; Diet/veterinary ; Ruminants/metabolism ; Amino Acids/metabolism ; Digestion
Chemical Substances	Dietary Proteins ; Amino Acids
Language	English
Publishing date	2024-04-01
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2095161-9
ISSN	1740-0929 ; 1344-3941
ISSN (online)	1740-0929
ISSN	1344-3941
DOI	10.1111/asj.13948
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Article ; Online: Immunohistochemical localization and possible physiological roles of tumor necrosis factor-α in mouse cumulus-oocyte complexes.

Sato, Eimei / Nakayama, Taisuke / Kamio, Kae / Takahashi, Yuji / Toyoda, Yutaka

Development, growth & differentiation

2023 Volume 37, Issue 4, Page(s) 413–420

Abstract: Tumor necrosis factor-α (TNF-α), a cytokine which is produced by activated macrophages, has been shown to participate in the regulation of ovarian functions. In the course of our investigation on the mechanism of maturation, fertilization and ... ...

Abstract	Tumor necrosis factor-α (TNF-α), a cytokine which is produced by activated macrophages, has been shown to participate in the regulation of ovarian functions. In the course of our investigation on the mechanism of maturation, fertilization and degeneration of mouse oocytes, immunoreactivity to TNF-α was found in the cytoplasm of the cells surrounding the maturing oocytes and of granulosa cells facing the antral cavity. Immunoblot analysis with the specific antibody to TNF-α identified the 17 kDa Mr band in the extract of cumulusoocyte complexes. Various concentrations of TNF-α (mouse, recombinant) and anti TNF-α antiserum (polyclonal rabbit anti-mouse recombinant TNF-α) were then used to determine their effect on the germinal vesicle breakdown (GVBD), polar body extrusion, fertilization and fragmentation of mouse oocytes/eggs. TNF-α at concentrations of 10 ng/mL or less and anti-TNF-α antiserum at concentrations of 10% or less, had no effect on the spontaneous GVBD and polar body extrusion of mouse oocytes in culture. Mouse follicular oocytes cultured for more than 72 h in modified Krebs-Ringer solution in vitro undergo spontaneous fragmentation, which is a degenerative change to form 'blastomeres' with or without nuclear fragments or chromatin. Ghost-like blastomeres were also identified in the space among fragmented 'blastomeres'. The spontaneous fragmentation of mouse follicular oocytes was suppressed in the presence of TNF-α at concentrations of 1 ng/mL or greater. Anti-TNF-α antiserum (1%) accelerated the induction of fragmentation of oocytes cultured in vitro. The addition of anti TNF-α antiserum (10%) to the culture medium did not influence the fertilization rates of the eggs surrounded by the expanded cumulus. These results appear to indicate that the process of degeneration of mouse oocytes/eggs is modulated by TNF-α accumulated in the expanded cumulus during oocyte maturation.
Language	English
Publishing date	2023-06-06
Publishing country	Japan
Document type	Journal Article
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1046/j.1440-169X.1995.t01-3-00008.x
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Article ; Online: Microdevice in Cellular Pathology: Microfluidic Platforms for Fluorescence in situ Hybridization and Analysis of Circulating Tumor Cells.

Sato, Kae

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

2015 Volume 31, Issue 9, Page(s) 867–873

Abstract: Microfluidic devices enable the miniaturization, integration, automation, and parallelization of chemical and biochemical processes. This new technology also provides opportunity for expansion in the field of cellular pathology. Fluorescence in situ ... ...

Abstract	Microfluidic devices enable the miniaturization, integration, automation, and parallelization of chemical and biochemical processes. This new technology also provides opportunity for expansion in the field of cellular pathology. Fluorescence in situ hybridization (FISH) is a well-known gene-based method to image genetic abnormalities. Development of a FISH microfluidic platform has offered the possibility of automation with significant time and cost reductions, which overcomes many drawbacks of the current protocols. Microfluidic devices are also powerful tools for single-cell analysis. Capturing the circulating tumor cells (CTCs) from blood samples is one of the most promising approaches to enable the early diagnosis of cancer. The microfluidic devices are also useful to isolate rare CTCs at high efficiency and purity. In this review, I outline recent FISH and CTC analyses using microfluidic devices, and describe their applications for the cellular diagnosis of cancers.
MeSH term(s)	Animals ; Humans ; In Situ Hybridization, Fluorescence/instrumentation ; In Situ Hybridization, Fluorescence/methods ; Microfluidic Analytical Techniques/instrumentation ; Microfluidic Analytical Techniques/methods ; Neoplastic Cells, Circulating/pathology
Language	English
Publishing date	2015
Publishing country	Japan
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1483376-1
ISSN	1348-2246 ; 0910-6340
ISSN (online)	1348-2246
ISSN	0910-6340
DOI	10.2116/analsci.31.867
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Article ; Online: Low-temperature plasma as magic wand to differentiate between the good and the evil.

Toyokuni, Shinya / Zheng, Hao / Kong, Yingyi / Sato, Kotaro / Nakamura, Kae / Tanaka, Hiromasa / Okazaki, Yasumasa

Free radical research

2023 Volume 57, Issue 1, Page(s) 38–46

Abstract: Plasma is the fourth physical state of matter, characterized by an ionized gaseous mixture, after solid, liquid, and gas phases, and contains a wide array of components such as ions, electrons, radicals, and ultraviolet ray. Whereas the sun and thunder ... ...

Abstract	Plasma is the fourth physical state of matter, characterized by an ionized gaseous mixture, after solid, liquid, and gas phases, and contains a wide array of components such as ions, electrons, radicals, and ultraviolet ray. Whereas the sun and thunder are typical natural plasma, recent progress in the electronics enabled the generation of body-temperature plasma, designated as low-temperature plasma (LTP) or non-thermal plasma since the 1990s. LTP has attracted the attention of researchers for possible biological and medical applications. All the living species on earth utilize water as essential media for solvents and molecular transport. Thus, biological application of LTP naturally intervenes water whether LTP is exposed directly or indirectly, where plasma-activated lactate (PAL) is a standard, containing H
MeSH term(s)	Humans ; Hydrogen Peroxide ; Temperature ; Electron Spin Resonance Spectroscopy ; Neoplasms ; Apoptosis
Chemical Substances	Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2023-03-22
Publishing country	England
Document type	Journal Article
ZDB-ID	1194130-3
ISSN	1029-2470 ; 1071-5762
ISSN (online)	1029-2470
ISSN	1071-5762
DOI	10.1080/10715762.2023.2190860
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Zs.A 2236: Show issues

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Article ; Online: Bead-based Padlock Rolling Circle Amplification under Molecular Crowding Conditions: The Effects of Crowder Charge and Size.

Sasaki, Naoki / Kase, Chikako / Sato, Kae

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

2021 Volume 37, Issue 5, Page(s) 727–732

Abstract: Bead-based padlock rolling circle amplification under molecular crowding conditions, which we have developed for ultrasensitive detection of DNA, is examined to improve the detection efficiency and sensitivity of the method as well as to gain insight ... ...

Abstract	Bead-based padlock rolling circle amplification under molecular crowding conditions, which we have developed for ultrasensitive detection of DNA, is examined to improve the detection efficiency and sensitivity of the method as well as to gain insight into the mechanism of the method. Both non-magnetic and magnetic sepharose microbeads were employed. Biotinylated DNA had to be pre-immobilized onto the microbeads in order to obtain products on the magnetic beads. The optimal concentration of biotinylated DNA was found to be about 5 μM, above which the number of products decreased. The effect of the crowder charge was examined, and neutral polymers were found to be effective on ligation and the hybridization step, while charged polymers were only effective on the hybridization step and inhibited the ligation and primer extension. The effect of the molecular weight of neutral dextran on the number of products was investigated, and the number of products was found to be increased with an increase in the molecular weight of dextran.
MeSH term(s)	DNA/genetics ; Magnetics ; Microspheres ; Nucleic Acid Amplification Techniques ; Nucleic Acid Hybridization
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2021-01-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1483376-1
ISSN	1348-2246 ; 1348-2246
ISSN (online)	1348-2246
ISSN	1348-2246
DOI	10.2116/analsci.20SCP14
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Article: Fabrication of a Gelatin-Based Microdevice for Vascular Cell Culture.

Sasaki, Satoko / Suzuki, Tomoko / Morikawa, Kyojiro / Matsusaki, Michiya / Sato, Kae

Micromachines

2022 Volume 14, Issue 1

Abstract: This study presents a novel technique for fabricating microfluidic devices with microbial transglutaminase-gelatin gels instead of polydimethylsiloxane (PDMS), in which flow culture simulates blood flow and a capillary network is incorporated for assays ... ...

Abstract	This study presents a novel technique for fabricating microfluidic devices with microbial transglutaminase-gelatin gels instead of polydimethylsiloxane (PDMS), in which flow culture simulates blood flow and a capillary network is incorporated for assays of vascular permeability or angiogenesis. We developed a gelatin-based device with a coverslip as the bottom, which allows the use of high-magnification lenses with short working distances, and we observed the differences in cell dynamics on gelatin, glass, and PDMS surfaces. The tubes of the gelatin microfluidic channel are designed to be difficult to pull out of the inlet hole, making sample introduction easy, and the gelatin channel can be manipulated from the cell introduction to the flow culture steps in a manner comparable to that of a typical PDMS channel. Human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs) were successfully co-cultured, resulting in structures that mimicked blood vessels with inner diameters ranging from 10 µm to 500 µm. Immunostaining and scanning electron microscopy results showed that the affinity of fibronectin for gelatin was stronger than that for glass or PDMS, making gelatin a suitable substrate for cell adhesion. The ability for microscopic observation at high magnification and the ease of sample introduction make this device easier to use than conventional gelatin microfluidics, and the above-mentioned small modifications in the device structure are important points that improve its convenience as a cell assay device.
Language	English
Publishing date	2022-12-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2620864-7
ISSN	2072-666X
ISSN	2072-666X
DOI	10.3390/mi14010107
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Article ; Online: Recent Progress in the Development of Microfluidic Vascular Models.

Sato, Kae / Sato, Kiichi

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

2018 Volume 34, Issue 7, Page(s) 755–764

Abstract: The blood vessel is part of the circulatory system, and systemic circulation provides the blood supply to all tissues. Arteries are pathways through which the blood is carried, and the capillaries have a key role in material exchange to maintain the ... ...

Abstract	The blood vessel is part of the circulatory system, and systemic circulation provides the blood supply to all tissues. Arteries are pathways through which the blood is carried, and the capillaries have a key role in material exchange to maintain the tissue environment. Blood vessels have structures appropriate for their functions, and their sizes and cell types are different. In this review, we introduced recent studies of the microfluidic vascular models. The model structures are classified mainly as poly(dimethylsiloxane) and hydrogel microchannels and self-assembled networks. Basic phenomena and functions were realized in vascular models, including fluid shear stress, cell strain, interstitial flow, endothelial permeation, angiogenesis, and thrombosis. In some models, endothelial cells were co-cultured with smooth muscle cells, pericytes, and fibroblasts in an extracellular matrix. Examples of vascular models involving the brain, lung, liver, kidney, placenta, and cancer were also introduced.
MeSH term(s)	Blood Vessels/chemistry ; Dimethylpolysiloxanes/chemistry ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry ; Microfluidic Analytical Techniques ; Models, Molecular
Chemical Substances	Dimethylpolysiloxanes ; Hydrogel, Polyethylene Glycol Dimethacrylate (25852-47-5) ; baysilon (63148-62-9)
Language	English
Publishing date	2018-07-12
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	1483376-1
ISSN	1348-2246 ; 0910-6340
ISSN (online)	1348-2246
ISSN	0910-6340
DOI	10.2116/analsci.17R006
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Article: Spatial variation in breeding phenology at small spatial scales: A stochastic effect of population size

Takahashi, Kae / Sato, Takuya

Population ecology. 2020 July, v. 62, no. 3

2020

Abstract: Spatial variation in phenology can occur at small spatial scales over which individuals can disperse or forage within one generation. Previous studies have assumed that variations in phenological peaks are caused by differences in abiotic environmental ... ...

Abstract	Spatial variation in phenology can occur at small spatial scales over which individuals can disperse or forage within one generation. Previous studies have assumed that variations in phenological peaks are caused by differences in abiotic environmental characteristics. However, environments should generally be similar among local habitats over small spatial scales. When the local population size is small, the phenological peak of the local population should be strongly affected by the variation in timing expressed by individuals. If a regional population consists of small local subpopulations (e.g., a metapopulation), the stochastic processes regulated by population sizes may explain the spatial variation in phenology. In this study, we quantitatively evaluated the extent of the spatial and annual variations in the breeding phenology of the forest green tree frog, Rhacophorus arboreus habiting a small area (<10 km²). The spatial variation in phenological peaks among 25 breeding sites was large over 6 years. This spatial variation was not explained by differences in air temperature or water depth. Randomization tests revealed that a large portion of the spatial variation could be explained by differences in population size, without considering site‐specific factors. Annual variations in phenological peaks tended to be greater for smaller populations. These results imply that the stochastic process might have caused the spatial and annual variations in the phenological peaks of R. arboreus observed in the study region. Understanding spatiotemporal variation in phenology determined by stochastic process would be important to better predict interspecific interactions and (meta)population dynamics at small spatial scales.
Keywords	Hylidae ; air temperature ; forage ; forests ; phenology ; population dynamics ; population size ; stochastic processes
Language	English
Dates of publication	2020-07
Size	p. 332-340.
Publishing place	John Wiley & Sons, Inc.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2015635-2
ISSN	1438-390X ; 1438-3896
ISSN (online)	1438-390X
ISSN	1438-3896
DOI	10.1002/1438-390X.12049
Database	NAL-Catalogue (AGRICOLA)

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Article: Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions.

Ishigaki, Yuri / Sato, Kae

Micromachines

2018 Volume 9, Issue 6

Abstract: The fluorescence in situ hybridization (FISH)-based padlock probe and rolling circle amplification (RCA) method allows for the detection of point mutations. However, it requires multiple reaction steps and solution exchanges, making it costly, labor- ... ...

Abstract	The fluorescence in situ hybridization (FISH)-based padlock probe and rolling circle amplification (RCA) method allows for the detection of point mutations. However, it requires multiple reaction steps and solution exchanges, making it costly, labor-intensive, and time-consuming. In this study, we aimed to improve the efficiency of padlock/RCA by determining the effects of microchannel shape and ultrasonic solution mixing. Using a circular-shaped microchamber and ultrasonic mixing, the efficiency of microfluidic padlock/RCA was improved, and the consumption of the expensive probe solution was reduced from 10 µL to approximately 3.5 µL. Moreover, the fluorescent probe hybridization time was reduced to 5 min, which is four times faster than that of the standard protocol. We used this method to successfully detect mitochondrial DNA and transcripts of β
Language	English
Publishing date	2018-05-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2620864-7
ISSN	2072-666X
ISSN	2072-666X
DOI	10.3390/mi9060272
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