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  1. Article: Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA.

    Varlamov, Dmitriy A / Blagodatskikh, Konstantin A / Smirnova, Evgenia V / Kramarov, Vladimir M / Ignatov, Konstantin B

    Frontiers in bioengineering and biotechnology

    2020  Volume 8, Page(s) 604793

    Abstract: The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of August 31, 2020. To aid in the development of diagnostic ... ...

    Abstract The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of August 31, 2020. To aid in the development of diagnostic kits for rapid and sensitive detection of the virus, we evaluated a combination of polymerase chain reaction (PCR) and isothermal nucleic acid amplification techniques. Here, we compared conventional PCR and loop-mediated isothermal amplification (LAMP) methods with hybrid techniques such as polymerase chain displacement reaction (PCDR) and a newly developed PCR-LAMP method. We found that the hybrid methods demonstrated higher sensitivity and assay reaction rates than those of the classic LAMP and PCR techniques and can be used to for SARS-CoV-2 detection. The proposed methods based on the modern hybrid amplification techniques markedly improve virus detection and, therefore, can be extremely useful in the development of new diagnostic kits.
    Language English
    Publishing date 2020-11-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2020.604793
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA

    Varlamov, Dmitriy A. / Blagodatskikh, Konstantin A. / Smirnova, Evgenia V. / Kramarov, Vladimir M. / Ignatov, Konstantin B.

    Frontiers in Bioengineering and Biotechnology

    2020  Volume 8

    Keywords Biotechnology ; Bioengineering ; Histology ; Biomedical Engineering ; covid19
    Publisher Frontiers Media SA
    Publishing country ch
    Document type Article ; Online
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2020.604793
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Combinations of PCR and isothermal amplification techniques are suitable for fast and sensitive detection of SARS-CoV-2 viral RNA

    Varlamov, Dmitriy A / Blagodatskikh, Konstantin A / Smirnova, Evgeniya V / Kramarov, Vladimir M. V / Ignatov, Konstantin B

    medRxiv

    Abstract: The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of 31 August 2020. To aid in the development of diagnostic ... ...

    Abstract The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of 31 August 2020. To aid in the development of diagnostic kits for rapid and sensitive detection of the virus, we evaluated a combination of polymerase chain reaction (PCR) and isothermal nucleic acid amplification techniques. Here, we compared conventional PCR and loop-mediated isothermal amplification (LAMP) methods with hybrid techniques such as polymerase chain displacement reaction (PCDR) and a newly developed PCR-LAMP method. We found that the hybrid methods demonstrated higher sensitivity and assay reaction rates than those of the classic LAMP and PCR techniques and can be used to for SARS-CoV-2 detection. The proposed methods based on the modern hybrid amplification techniques markedly improve virus detection and, therefore, can be extremely useful in the development of new diagnostic kits.
    Keywords covid19
    Language English
    Publishing date 2020-09-25
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2020.09.23.20200055
    Database COVID19

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  4. Article ; Online: Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA

    Dmitriy A. Varlamov / Konstantin A. Blagodatskikh / Evgenia V. Smirnova / Vladimir M. Kramarov / Konstantin B. Ignatov

    Frontiers in Bioengineering and Biotechnology, Vol

    2020  Volume 8

    Abstract: The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of August 31, 2020. To aid in the development of diagnostic ... ...

    Abstract The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of August 31, 2020. To aid in the development of diagnostic kits for rapid and sensitive detection of the virus, we evaluated a combination of polymerase chain reaction (PCR) and isothermal nucleic acid amplification techniques. Here, we compared conventional PCR and loop-mediated isothermal amplification (LAMP) methods with hybrid techniques such as polymerase chain displacement reaction (PCDR) and a newly developed PCR-LAMP method. We found that the hybrid methods demonstrated higher sensitivity and assay reaction rates than those of the classic LAMP and PCR techniques and can be used to for SARS-CoV-2 detection. The proposed methods based on the modern hybrid amplification techniques markedly improve virus detection and, therefore, can be extremely useful in the development of new diagnostic kits.
    Keywords SARS-CoV-2 ; COVID-19 ; loop-mediated isothermal amplification ; polymerase chain displacement reaction ; PCR-LAMP ; hybrid virus detection technique ; Biotechnology ; TP248.13-248.65 ; covid19
    Subject code 572
    Language English
    Publishing date 2020-11-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Book ; Online: Table_1_Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA.DOCX

    Dmitriy A. Varlamov / Konstantin A. Blagodatskikh / Evgenia V. Smirnova / Vladimir M. Kramarov / Konstantin B. Ignatov

    2020  

    Abstract: The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of August 31, 2020. To aid in the development of diagnostic ... ...

    Abstract The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of August 31, 2020. To aid in the development of diagnostic kits for rapid and sensitive detection of the virus, we evaluated a combination of polymerase chain reaction (PCR) and isothermal nucleic acid amplification techniques. Here, we compared conventional PCR and loop-mediated isothermal amplification (LAMP) methods with hybrid techniques such as polymerase chain displacement reaction (PCDR) and a newly developed PCR-LAMP method. We found that the hybrid methods demonstrated higher sensitivity and assay reaction rates than those of the classic LAMP and PCR techniques and can be used to for SARS-CoV-2 detection. The proposed methods based on the modern hybrid amplification techniques markedly improve virus detection and, therefore, can be extremely useful in the development of new diagnostic kits.
    Keywords Biotechnology ; Biological Engineering ; Genetic Engineering ; Biomarkers ; Biomaterials ; Biomechanical Engineering ; Biomedical Engineering not elsewhere classified ; Synthetic Biology ; Agricultural Marine Biotechnology ; Bioremediation ; Bioprocessing ; Bioproduction and Bioproducts ; Industrial Biotechnology Diagnostics (incl. Biosensors) ; Industrial Microbiology (incl. Biofeedstocks) ; Industrial Molecular Engineering of Nucleic Acids and Proteins ; Industrial Biotechnology not elsewhere classified ; Medical Biotechnology Diagnostics (incl. Biosensors) ; Medical Molecular Engineering of Nucleic Acids and Proteins ; Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; Medical Biotechnology not elsewhere classified ; SARS-CoV-2 ; COVID-19 ; loop-mediated isothermal amplification ; polymerase chain displacement reaction ; PCR-LAMP ; hybrid virus detection technique ; covid19
    Publishing date 2020-11-04T04:14:01Z
    Publishing country uk
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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  6. Article: Easy construction of furo[2,3-f]isoindole core by the IMDAV reaction between 3-(furyl)allylamines and α,β-unsaturated acid anhydrides

    Zubkov, Fedor I / Alexey V. Varlamov / Dmitriy F. Mertsalov / Eugenia V. Nikitina / Mykola D. Obushak / Pavel V. Dorovatovskii / Roman Z. Lytvyn / Victor N. Khrustalev / Vladimir P. Zaytsev / Yuriy I. Horak / Yuriy V. Homza

    Tetrahedron. 2016 May 05, v. 72

    2016  

    Abstract: The vinylogues of furfurylamines, easily available in two steps from furylacroleins (3-(furyl)allylamines), were studied in a tandem N-acylation/intramolecular [4+2] cycloaddition with maleic, pyrocinchonic, and citraconic anhydrides, as well as ... ...

    Abstract The vinylogues of furfurylamines, easily available in two steps from furylacroleins (3-(furyl)allylamines), were studied in a tandem N-acylation/intramolecular [4+2] cycloaddition with maleic, pyrocinchonic, and citraconic anhydrides, as well as furylacryloyl and cinnamoyl chlorides. By using a domino reaction of 3-(furyl)allylamines and α,β-unsaturated acid anhydrides under mild conditions, various hexahydro-4H-furo[2,3-f]isoindoles and their carboxyl derivatives were synthesized efficiently. The domino sequence includes three steps: acylation of the nitrogen atom in 3-(furyl)allylamines, intramolecular Diels–Alder cycloaddition in the resulting N-acyl vinylfurans (IMDAV reaction), and prototropic shift of the adducts followed by recovery of aromaticity of the furan nucleus. The key step, IMDAV reaction, leads to the target products, furo[2,3-f]isoindoles, diastereoselectively and with relatively high yields.
    Keywords acylation ; anhydrides ; chemical structure ; chlorides ; cycloaddition reactions ; nitrogen
    Language English
    Dates of publication 2016-0505
    Size p. 2239-2253.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 204285-x
    ISSN 1464-5416 ; 0040-4020 ; 0563-2064
    ISSN (online) 1464-5416
    ISSN 0040-4020 ; 0563-2064
    DOI 10.1016/j.tet.2016.03.023
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1076: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  7. Article: The intramolecular Diels–Alder vinylfuran (IMDAV) reaction: a short approach to aza-analogues of pinguisane-type sesquiterpenes

    Horak, Yuriy I / Alexey V. Varlamov / Dmitriy F. Mertsalov / Eugenia V. Nikitina / Fedor I. Zubkov / Maria N. Babkina / Mykola D. Obushak / Roman Z. Lytvyn / Tadeusz Lis / Vasyl Kinzhybalo / Vasyl S. Matiychuk / Vladimir P. Zaytsev / Yuriy V. Homza

    Tetrahedron letters. 2015 July 22, v. 56, no. 30

    2015  

    Abstract: The reaction of readily accessible (3-furyl)allylamines with maleic anhydride, followed by a domino sequence involving acylation/cycloaddition/proton shift steps, led to the formation of furo[2,3-f]isoindoles—the aza-analogs of pinguisane-type ... ...

    Abstract The reaction of readily accessible (3-furyl)allylamines with maleic anhydride, followed by a domino sequence involving acylation/cycloaddition/proton shift steps, led to the formation of furo[2,3-f]isoindoles—the aza-analogs of pinguisane-type sesquiterpenes. The key intramolecular Diels–Alder vinylfuran (IMDAV) reaction proceeded under mild conditions, with high levels of diastereoselectivity and satisfactory yields.
    Keywords acylation ; chemical structure ; cycloaddition reactions ; diastereoselectivity ; maleic anhydrides ; sesquiterpenoids
    Language English
    Dates of publication 2015-0722
    Size p. 4499-4501.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 204287-3
    ISSN 1873-3581 ; 0040-4039
    ISSN (online) 1873-3581
    ISSN 0040-4039
    DOI 10.1016/j.tetlet.2015.05.115
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2837: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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