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Article ; Online: Smallpox vaccination-elicited antibodies cross-neutralize 2022-Monkeypox virus Clade II.

Criscuolo, Elena / Giuliani, Benedetta / Ferrarese, Roberto / Ferrari, Davide / Locatelli, Massimo / Clementi, Massimo / Mancini, Nicasio / Clementi, Nicola

Journal of medical virology

2023 Volume 95, Issue 3, Page(s) e28643

Abstract: Since early May 2022, some monkeypox virus (MPXV) infections have been reported from countries where the disease is not endemic. Within 2 months, the number of patients has increased extensively, becoming the most considerable MPXV outbreak described. ... ...

Abstract	Since early May 2022, some monkeypox virus (MPXV) infections have been reported from countries where the disease is not endemic. Within 2 months, the number of patients has increased extensively, becoming the most considerable MPXV outbreak described. Smallpox vaccines demonstrated high efficacy against MPXVs in the past and are considered a crucial outbreak control measure. However, viruses isolated during the current outbreak carry distinct genetic variations, and the cross-neutralizing capability of antibodies remains to be assessed. Here we report that serum antibodies elicited by first-generation smallpox vaccines can neutralize the current MPXV more than 40 years after vaccine administration.
MeSH term(s)	Humans ; Monkeypox virus ; Mpox (monkeypox)/epidemiology ; Mpox (monkeypox)/prevention & control ; Smallpox ; Smallpox Vaccine/genetics ; Vaccination
Chemical Substances	Smallpox Vaccine
Language	English
Publishing date	2023-03-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.28643
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Zs.A 1320: Show issues

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Article ; Online: SARS-CoV-2 infection despite high levels of vaccine-induced anti-Receptor-Binding-Domain antibodies: a study on 1110 health-care professionals from a northern Italian university hospital.

Ferrari, Davide / Clementi, Nicola / Mancini, Nicasio / Locatelli, Massimo

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

2021 Volume 28, Issue 2, Page(s) 305–307

MeSH term(s)	Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 ; Hospitals ; Humans ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Vaccines
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; Vaccines
Language	English
Publishing date	2021-10-29
Publishing country	England
Document type	Letter
ZDB-ID	1328418-6
ISSN	1469-0691 ; 1470-9465 ; 1198-743X
ISSN (online)	1469-0691
ISSN	1470-9465 ; 1198-743X
DOI	10.1016/j.cmi.2021.10.010
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Zs.A 4541: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 284: Show issues

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Article ; Online: Dual-Domain Reporter Approach for Multiplex Identification of Major SARS-CoV-2 Variants of Concern in a Microarray-Based Assay.

Damin, Francesco / Galbiati, Silvia / Clementi, Nicola / Ferrarese, Roberto / Mancini, Nicasio / Sola, Laura / Chiari, Marcella

Biosensors

2023 Volume 13, Issue 2

Abstract: Since the emergence of the COVID-19 pandemic in December 2019, the SARS-CoV-2 virus continues to evolve into many variants emerging around the world. To enable regular surveillance and timely adjustments in public health interventions, it is of the ... ...

Abstract	Since the emergence of the COVID-19 pandemic in December 2019, the SARS-CoV-2 virus continues to evolve into many variants emerging around the world. To enable regular surveillance and timely adjustments in public health interventions, it is of the utmost importance to accurately monitor and track the distribution of variants as rapidly as possible. Genome sequencing is the gold standard for monitoring the evolution of the virus, but it is not cost-effective, rapid and easily accessible. We have developed a microarray-based assay that can distinguish known viral variants present in clinical samples by simultaneously detecting mutations in the Spike protein gene. In this method, the viral nucleic acid, extracted from nasopharyngeal swabs, after RT-PCR, hybridizes in solution with specific dual-domain oligonucleotide reporters. The domains complementary to the Spike protein gene sequence encompassing the mutation form hybrids in solution that are directed by the second domain ("barcode" domain) at specific locations on coated silicon chips. The method utilizes characteristic fluorescence signatures to unequivocally differentiate, in a single assay, different known SARS-CoV-2 variants. In the nasopharyngeal swabs of patients, this multiplex system was able to genotype the variants which have caused waves of infections worldwide, reported by the WHO as being of concern (VOCs), namely Alpha, Beta, Gamma, Delta and Omicron variants.
MeSH term(s)	Humans ; SARS-CoV-2 ; COVID-19 ; Pandemics ; Spike Glycoprotein, Coronavirus
Chemical Substances	Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2023-02-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios13020269
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Article ; Online: A case of breakthrough mpox infection in an individual non-responder to MVA-BN vaccination.

Bottanelli, Martina / Messina, Emanuela / Raccagni, Angelo Roberto / Moschetta, Nicolò / Clementi, Nicola / Muccini, Camilla / Castagna, Antonella / Nozza, Silvia

The Lancet. Infectious diseases

2023 Volume 24, Issue 1, Page(s) e11–e12

MeSH term(s)	Humans ; Mpox (monkeypox) ; Vaccination ; Vaccinia virus
Chemical Substances	smallpox and monkeypox vaccine modified vaccinia ankara-bavarian nordic (TU8J357395)
Language	English
Publishing date	2023-12-07
Publishing country	United States
Document type	Case Reports ; Letter
ZDB-ID	2061641-7
ISSN	1474-4457 ; 1473-3099
ISSN (online)	1474-4457
ISSN	1473-3099
DOI	10.1016/S1473-3099(23)00741-7
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Zs.A 5743: Show issues

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Article ; Online: Proper Selection of In Vitro Cell Model Affects the Characterization of the Neutralizing Antibody Response against SARS-CoV-2.

Criscuolo, Elena / Giuliani, Benedetta / Ferrari, Davide / Ferrarese, Roberto / Diotti, Roberta A / Clementi, Massimo / Mancini, Nicasio / Clementi, Nicola

Viruses

2022 Volume 14, Issue 6

Abstract: 1) Background: Our aim is the evaluation of the neutralizing activity of BNT162b2 mRNA vaccine-induced antibodies in different in vitro cellular models, as this still represents one of the surrogates of protection against SARS-CoV-2 viral variants. (2) ... ...

Abstract	(1) Background: Our aim is the evaluation of the neutralizing activity of BNT162b2 mRNA vaccine-induced antibodies in different in vitro cellular models, as this still represents one of the surrogates of protection against SARS-CoV-2 viral variants. (2) Methods: The entry mechanisms of SARS-CoV-2 in three cell lines (Vero E6, Vero E6/TMPRSS2 and Calu-3) were evaluated with both pseudoviruses and whole virus particles. The neutralizing capability of sera collected from vaccinated subjects was characterized through cytopathic effects and Real-Time RT PCR. (3) Results: In contrast to Vero E6 and Vero E6/TMPRSS2, Calu-3 allowed the evaluation of both viral entry mechanisms, resembling what occurs during natural infection. The choice of an appropriate cellular model can decisively influence the determination of the neutralizing activity of antibodies against SARS-CoV-2 variants. Indeed, the lack of correlation between neutralizing data in Calu-3 and Vero E6 demonstrated that testing the antibody inhibitory activity by using a single cell model possibly results in an inaccurate characterization. (4) Conclusions: Cellular systems allowing only one of the two viral entry pathways may not fully reflect the neutralizing activity of vaccine-induced antibodies moving increasingly further away from possible correlates of protection from SARS-CoV-2 infection.
MeSH term(s)	Animals ; Antibodies, Neutralizing ; Antibodies, Viral ; BNT162 Vaccine ; COVID-19 ; Chlorocebus aethiops ; Humans ; SARS-CoV-2 ; Vaccines, Synthetic ; Vero Cells ; mRNA Vaccines
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; Vaccines, Synthetic ; mRNA Vaccines ; BNT162 Vaccine (N38TVC63NU)
Language	English
Publishing date	2022-06-07
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v14061232
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Article ; Online: Trends of Antimicrobial Susceptibility of Neisseria gonorrhoeae isolates between 2012 and 2023: results from an open Italian cohort.

Lucente, Maria Francesca / Raccagni, Angelo Roberto / Galli, Laura / Lolatto, Riccardo / Ranzenigo, Martina / Ripa, Marco / Ponta, Giacomo / Monardo, Roberta / Gona, Floriana / Clementi, Nicola / Burioni, Roberto / Carletti, Silvia / Castagna, Antonella / Nozza, Silvia

Sexually transmitted diseases

2024

Abstract: Background: Neisseria gonorrhoeae (Ng) is a public health priority due to the rapid evolution of antimicrobial resistance, the emergence of antibiotic resistance and the absence of a vaccine against Ng. The aim of this study was to investigate trends in ...

Abstract	Background: Neisseria gonorrhoeae (Ng) is a public health priority due to the rapid evolution of antimicrobial resistance, the emergence of antibiotic resistance and the absence of a vaccine against Ng. The aim of this study was to investigate trends in the minimum inhibitory concentration (MIC) and resistance (R) or reduced susceptibility (DS) of Ng cases to ceftriaxone (CRO), azithromycin (AZM), tetracycline (TET), benzylpenicillin (PenG), ciprofloxacin (CIP) over a 10-year period. Methods: Retrospective analysis on an open cohort of Ng cases diagnosed on rectal, urethral and pharyngeal samples at San Raffaele Scientific Institute, between September 2012-February 2023. MICs of antibiotics were determined by gradient-test strips. Bivariate linear regression models, applied on logarithmic MICs values; Cochran-Armitage test was used to determine a linear trend in the proportions of resistant strains. Results: 436 Ng isolates from 352 individuals were analyzed. MICs of CRO and PenG reduced over time (p < 0.001, p = 0.030), AZM increased (p = 0.001), CIP and TET did not change (p = 0.473, p = 0.272). The percentages of resistant strains were: PenG 89.9%, TET 90.8%, CIP 48.2%, AZM 4.4%; CRO-DS strains were 8.7%, only one CRO-R. The proportion of resistant strains increased over time for AZM (p = 0.007), TET (p = 0.001), CIP (p < 0.001), whereas decreased for PenG (p < 0.001) and CRO-DS/R strains (p < 0.001). Conclusions: Ng strains showed high susceptibility to CRO, although we identified cases of DS/R and observed high levels of susceptibility to AZM. Overall, the recommended primary regimen for Ng treatment confirmed to be effective.
Language	English
Publishing date	2024-04-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	435191-5
ISSN	1537-4521 ; 0148-5717
ISSN (online)	1537-4521
ISSN	0148-5717
DOI	10.1097/OLQ.0000000000001981
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Zs.A 1217: Show issues

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Article ; Online: Very high SARS-CoV-2 load at the emergency department presentation strongly predicts the risk of admission to the intensive care unit and death.

Mancini, Nicasio / Clementi, Nicola / Ferrarese, Roberto / Ambrosi, Alessandro / Tonelli, Marco / Zangrillo, Alberto / Landoni, Giovanni / Clementi, Massimo

Clinical chemistry and laboratory medicine

2021 Volume 59, Issue 6, Page(s) e247–e250

MeSH term(s)	Aged ; COVID-19/diagnosis ; COVID-19/mortality ; COVID-19 Nucleic Acid Testing ; Emergency Service, Hospital/statistics & numerical data ; Female ; Humans ; Intensive Care Units/statistics & numerical data ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Multivariate Analysis ; Nasopharynx/virology ; Prognosis ; Proportional Hazards Models ; RNA, Viral/analysis ; SARS-CoV-2/chemistry ; Viral Load/statistics & numerical data
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-01-22
Publishing country	Germany
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2020-1709
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Zs.A 121: Show issues

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Article ; Online: Fast inactivation of SARS-CoV-2 by UV-C and ozone exposure on different materials.

Criscuolo, Elena / Diotti, Roberta A / Ferrarese, Roberto / Alippi, Cesare / Viscardi, Gabriele / Signorelli, Carlo / Mancini, Nicasio / Clementi, Massimo / Clementi, Nicola

Emerging microbes & infections

2021 Volume 10, Issue 1, Page(s) 206–210

Abstract: The extremely rapid spread of the SARS-CoV-2 has already resulted in more than 1 million reported deaths of coronavirus disease 2019 (COVID-19). The ability of infectious particles to persist on environmental surfaces is potentially considered a factor ... ...

Abstract	The extremely rapid spread of the SARS-CoV-2 has already resulted in more than 1 million reported deaths of coronavirus disease 2019 (COVID-19). The ability of infectious particles to persist on environmental surfaces is potentially considered a factor for viral spreading. Therefore, limiting viral diffusion in public environments should be achieved with correct disinfection of objects, tissues, and clothes. This study proves how two widespread disinfection systems, short-wavelength ultraviolet light (UV-C) and ozone (O3), are active in vitro on different commonly used materials. The development of devices equipped with UV-C, or ozone generators, may prevent the virus from spreading in public places.
MeSH term(s)	COVID-19/prevention & control ; Disinfection/methods ; Humans ; Ozone/pharmacology ; SARS-CoV-2/drug effects ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/radiation effects ; Ultraviolet Rays ; Virus Inactivation/drug effects ; Virus Inactivation/radiation effects
Chemical Substances	Ozone (66H7ZZK23N)
Language	English
Publishing date	2021-01-04
Publishing country	United States
Document type	Letter
ZDB-ID	2681359-2
ISSN	2222-1751 ; 2222-1751
ISSN (online)	2222-1751
ISSN	2222-1751
DOI	10.1080/22221751.2021.1872354
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Article: Proper Selection of In Vitro Cell Model Affects the Characterization of the Neutralizing Antibody Response against SARS-CoV-2

Criscuolo, Elena / Giuliani, Benedetta / Ferrari, Davide / Ferrarese, Roberto / Diotti, Roberta A. / Clementi, Massimo / Mancini, Nicasio / Clementi, Nicola

Viruses. 2022 June 07, v. 14, no. 6

2022

Abstract	(1) Background: Our aim is the evaluation of the neutralizing activity of BNT162b2 mRNA vaccine-induced antibodies in different in vitro cellular models, as this still represents one of the surrogates of protection against SARS-CoV-2 viral variants. (2) Methods: The entry mechanisms of SARS-CoV-2 in three cell lines (Vero E6, Vero E6/TMPRSS2 and Calu-3) were evaluated with both pseudoviruses and whole virus particles. The neutralizing capability of sera collected from vaccinated subjects was characterized through cytopathic effects and Real-Time RT PCR. (3) Results: In contrast to Vero E6 and Vero E6/TMPRSS2, Calu-3 allowed the evaluation of both viral entry mechanisms, resembling what occurs during natural infection. The choice of an appropriate cellular model can decisively influence the determination of the neutralizing activity of antibodies against SARS-CoV-2 variants. Indeed, the lack of correlation between neutralizing data in Calu-3 and Vero E6 demonstrated that testing the antibody inhibitory activity by using a single cell model possibly results in an inaccurate characterization. (4) Conclusions: Cellular systems allowing only one of the two viral entry pathways may not fully reflect the neutralizing activity of vaccine-induced antibodies moving increasingly further away from possible correlates of protection from SARS-CoV-2 infection.
Keywords	Pseudoviridae ; Severe acute respiratory syndrome coronavirus 2 ; antibody formation ; models ; viruses
Language	English
Dates of publication	2022-0607
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v14061232
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Viral Respiratory Pathogens and Lung Injury.

Clementi, Nicola / Ghosh, Sreya / De Santis, Maria / Castelli, Matteo / Criscuolo, Elena / Zanoni, Ivan / Clementi, Massimo / Mancini, Nicasio

Clinical microbiology reviews

2021 Volume 34, Issue 3

Abstract: Several viruses target the human respiratory tract, causing different clinical manifestations spanning from mild upper airway involvement to life-threatening acute respiratory distress syndrome (ARDS). As dramatically evident in the ongoing SARS-CoV-2 ... ...

Abstract	Several viruses target the human respiratory tract, causing different clinical manifestations spanning from mild upper airway involvement to life-threatening acute respiratory distress syndrome (ARDS). As dramatically evident in the ongoing SARS-CoV-2 pandemic, the clinical picture is not always easily predictable due to the combined effect of direct viral and indirect patient-specific immune-mediated damage. In this review, we discuss the main RNA (orthomyxoviruses, paramyxoviruses, and coronaviruses) and DNA (adenoviruses, herpesviruses, and bocaviruses) viruses with respiratory tropism and their mechanisms of direct and indirect cell damage. We analyze the thin line existing between a protective immune response, capable of limiting viral replication, and an unbalanced, dysregulated immune activation often leading to the most severe complication. Our comprehension of the molecular mechanisms involved is increasing and this should pave the way for the development and clinical use of new tailored immune-based antiviral strategies.
MeSH term(s)	Adult ; Aged ; Antiviral Agents/therapeutic use ; COVID-19 ; Child ; Child, Preschool ; DNA Viruses ; Female ; Humans ; Immunologic Factors/therapeutic use ; Infant ; Infant, Newborn ; Interferons/therapeutic use ; Lung/immunology ; Lung/virology ; Lung Injury/diagnosis ; Lung Injury/drug therapy ; Lung Injury/immunology ; Lung Injury/virology ; Male ; Middle Aged ; Pandemics ; RNA Viruses ; Respiratory Tract Infections ; SARS-CoV-2 ; Virus Diseases
Chemical Substances	Antiviral Agents ; Immunologic Factors ; Interferons (9008-11-1)
Language	English
Publishing date	2021-03-31
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	645015-5
ISSN	1098-6618 ; 0893-8512
ISSN (online)	1098-6618
ISSN	0893-8512
DOI	10.1128/CMR.00103-20
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