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Article ; Online: Effectiveness of a grid mattress on adults' sleep quality and health: A quasi-experimental intervention study.

Breus, Michael / Hooper, Stephanie L / Lynch, Tarah / Barragan, Martin / Hausenblas, Heather A

Health science reports

2024 Volume 7, Issue 4, Page(s) e2046

Abstract: Background and aims: Despite that 93% of people indicate that a mattress plays a pivotal role in achieving high-quality sleep, there is a scarcity of research investigating the influence of mattresses on sleep quality, pain, and mood in nonclinical poor ...

Abstract	Background and aims: Despite that 93% of people indicate that a mattress plays a pivotal role in achieving high-quality sleep, there is a scarcity of research investigating the influence of mattresses on sleep quality, pain, and mood in nonclinical poor sleepers. The purpose was to examine the effectiveness of a pressure-releasing medium-firm grid mattress on sleep and health outcomes (e.g., mood, pain, daytime fatigue) of adults with nonclinical insomnia symptoms using a quasi-experimental design. Methods: Participants were 39 adults (mean age = 45.29) with nonclinical insomnia (i.e., occasional sleeplessness). Following 1 week of baseline assessments on their current mattress, they slept on a pressure-relieving grid mattress for 8 weeks. Participants completed self-report assessments of the Pittsburgh Sleep Quality Index, Berlin Questionnaire, Insomnia Severity Index, Restorative Sleep Questionnaire, Perceived Stress Scale, Profile of Mood States, Daytime Fatigue Scale, Pain and Sleep Questionnaire, and Brief Pain Inventory at Baseline and Weeks 1, 2, 3, 4, and 8. Participants continually wore an Oura Ring to objectively assess sleep and daytime activity. The data were collected from January 2022 to April 2022 and were stored electronically. Repeated-measures analyses of variance were used to analyze mean time differences. Results: Self-reported sleep quality, perceived pain, perceived stress, mood, and daytime fatigue improved significantly from Baseline to Week 8, Conclusion: The grid mattress is a simple, noninvasive, and nonpharmacological intervention that improved adults sleep quality and health. Controlled trials are encouraged to examine the effects of this mattress in a variety of populations and environments.
Language	English
Publishing date	2024-04-18
Publishing country	United States
Document type	Journal Article
ISSN	2398-8835
ISSN (online)	2398-8835
DOI	10.1002/hsr2.2046
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Genomic analysis of group A

Lynch, Tarah / Nandi, Tannistha / Jayaprakash, Teenus / Gregson, Dan / Church, Deirdre L

Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada

2022 Volume 7, Issue 1, Page(s) 23–35

Abstract: Background: In 2004-2005, an outbreak of impetigo occurred at a correctional facility during a sentinel outbreak of methicillin- resistant : Methods: Superficial wound swabs collected from all adults with impetigo during this outbreak were cultured ... ...

Abstract	Background: In 2004-2005, an outbreak of impetigo occurred at a correctional facility during a sentinel outbreak of methicillin- resistant Methods: Superficial wound swabs collected from all adults with impetigo during this outbreak were cultured using standard methods. NGS was used to characterize and compare all of the GAS and Results: Fifty-three adults were culture positive for GAS, with a subset of specimens also positive for MRSA ( Conclusions: GAS recovered from invasive and non-invasive sources were not genetically distinguishable. Virulence factors, plasmids, and AMR profiles grouped by
Language	English
Publishing date	2022-02-24
Publishing country	Canada
Document type	Journal Article
ISSN	2371-0888
ISSN (online)	2371-0888
DOI	10.3138/jammi-2021-0018
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Article: Efficacy of Wholetones

Hausenblas, Heather / Hooper, Stephanie / Hooper, David / Coyle, Kevin / Lynch, Tarah

Sleep science (Sao Paulo, Brazil)

2020 Volume 12, Issue 4, Page(s) 302–306

Abstract: Objectives: To conduct a randomized single-blind placebo-controlled crossover trial on adults with insomnia symptoms to examine the efficacy of Wholetones: Methods: Following baseline assessments, 38 adults (M age = 46.6 years) were randomized to ... ...

Abstract	Objectives: To conduct a randomized single-blind placebo-controlled crossover trial on adults with insomnia symptoms to examine the efficacy of Wholetones Methods: Following baseline assessments, 38 adults (M age = 46.6 years) were randomized to either WM or CM conditions for 10 days and then the alternative music for 10 days after a 4 day "wash-out". The outcomes were sleep quality (i.e., Pittsburgh Sleep Quality Index) and psychological measures. Results: Listening to both the WM and CM resulted in significant improvements from baseline for sleep quality, stress/anxiety, fatigue, productivity, and mood ( Conclusion: WM and CM provides a simple, noninvasive, and non-pharmacological intervention to promote sleep quality resulting in improved daytime mood, fatigue, productivity, and anxiety/stress.
Language	English
Publishing date	2020-04-15
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2697016-8
ISSN	1984-0063 ; 1984-0659
ISSN (online)	1984-0063
ISSN	1984-0659
DOI	10.5935/1984-0063.20190091
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Article ; Online: Spatial distribution of Escherichia coli ST131 C subclades in a centralized Canadian urban region.

Nobrega, Diego / Peirano, Gisele / Lynch, Tarah / Finn, Thomas J / Devinney, Rebekah / Pitout, Johann D D

The Journal of antimicrobial chemotherapy

2021 Volume 76, Issue 5, Page(s) 1135–1139

Abstract: Introduction: Escherichia coli ST131 is the most common multidrug-resistant (MDR) E. coli clone causing bloodstream infections (BSIs) in Calgary. This study describes patient characteristics and spatial distribution of ST131 subclades C1 and C2 causing ... ...

Abstract	Introduction: Escherichia coli ST131 is the most common multidrug-resistant (MDR) E. coli clone causing bloodstream infections (BSIs) in Calgary. This study describes patient characteristics and spatial distribution of ST131 subclades C1 and C2 causing BSIs in Calgary. Methods: E. coli from blood (n = 685) obtained in Calgary, Canada, (2016) were PCR screened for ST131 and positives (n = 141) underwent whole genome sequencing. Patient characteristics were analysed using Fisher's Exact/t-tests and spatial analysis was used to identify clusters. Results: Overall, 21% of E. coli was identified as ST131 and clade C dominated the population. ST131-C2 was associated with blaCTX-M-15 and significantly more MDR than ST131-C1. The spatial distribution in Calgary showed that ST131-C1 was mainly present in long-term care (LTC) residents whereas ST131-C2 clustered in a specific North East (NE) Calgary sector comprising of six neighbourhoods without LTC centres. This NE sector has high immigration and travel rates from the Indian subcontinent. Conclusions: This study showed that ST131 C subclades have different geographical distribution patterns in Calgary. We believe that recent travel to and immigration from certain high-risk regions for antimicrobial resistance are responsible for the ST131-C2 NE Calgary clustering pattern.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Canada/epidemiology ; Drug Resistance, Multiple, Bacterial ; Escherichia coli/genetics ; Escherichia coli Infections/epidemiology ; Escherichia coli Proteins/genetics ; Humans ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; Escherichia coli Proteins ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2021-02-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dkab020
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Zs.A 1197: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Effectiveness of NexQuest Natural Frequency Technology

Hooper, Stephanie / Lynch, Tarah / Coyle, Kevin / Hooper, David / Hausenblas, Heather A

Sleep science (Sao Paulo, Brazil)

2020 Volume 13, Issue 1, Page(s) 84–87

Abstract: Objectives: The study purpose was to conduct a four-week randomized double-blind placebo-controlled crossover trial on adults with insomnia symptoms to examine the effectiveness of Natural Frequency Technology: Methods: Adults (: Results: When the ...

Abstract	Objectives: The study purpose was to conduct a four-week randomized double-blind placebo-controlled crossover trial on adults with insomnia symptoms to examine the effectiveness of Natural Frequency Technology Methods: Adults ( Results: When the participants wore the SB, compared to the PB, they had improved sleep quality (i.e., Pittsburgh Sleep Quality Index), anxiety/perceived stress, and mood, Discussion: The SB may be simple, noninvasive, and non-pharmacological intervention to improve sleep quality and daytime mood.
Language	English
Publishing date	2020-07-01
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2697016-8
ISSN	1984-0063 ; 1984-0659
ISSN (online)	1984-0063
ISSN	1984-0659
DOI	10.5935/1984-0063.20190125
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Article ; Online: Tracking SARS-CoV-2 Omicron lineages using real-time reverse transcriptase PCR assays and prospective comparison with genome sequencing.

Zelyas, Nathan / Pabbaraju, Kanti / Croxen, Matthew A / Lynch, Tarah / McCullough, Emily / Murphy, Stephanie A / Shokoples, Sandy / Wong, Anita / Kanji, Jamil N / Tipples, Graham

Scientific reports

2023 Volume 13, Issue 1, Page(s) 17478

Abstract: Omicron has become the dominant SARS-CoV-2 variant globally since December 2021, with distinct waves being associated with separate Omicron sublineages. Rapid detection of BA.1, BA.2, BA.4, and BA.5 was accomplished in the province of Alberta, Canada, ... ...

Abstract	Omicron has become the dominant SARS-CoV-2 variant globally since December 2021, with distinct waves being associated with separate Omicron sublineages. Rapid detection of BA.1, BA.2, BA.4, and BA.5 was accomplished in the province of Alberta, Canada, through the design and implementation of real-time reverse transcriptase PCR assays targeting S:N501Y, S:ins214EPE, S:H69/V70, ORF7b:L11F, and M:D3N. Using the combination of results for each of these markers, samples could be designated as belonging to sublineages within BA.1, BA.2, BA.4, or BA.5. The analytical sensitivity of these markers ranged from 132 to 2229 copies/mL and in-laboratory accuracy was 98.9-100%. A 97.3% agreement using 12,592 specimens was demonstrated for the assays compared to genome sequencing. The use of these assays, combined with genome sequencing, facilitated the surveillance of SARS-CoV-2 lineages throughout a BA.5-dominated period.
MeSH term(s)	Humans ; SARS-CoV-2/genetics ; RNA-Directed DNA Polymerase ; Reverse Transcriptase Polymerase Chain Reaction ; COVID-19 ; Alberta ; COVID-19 Testing
Chemical Substances	RNA-Directed DNA Polymerase (EC 2.7.7.49)
Language	English
Publishing date	2023-10-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-44796-y
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Article ; Online: Tracking SARS-CoV-2 Omicron lineages using real-time reverse transcriptase PCR assays and prospective comparison with genome sequencing

Nathan Zelyas / Kanti Pabbaraju / Matthew A. Croxen / Tarah Lynch / Emily McCullough / Stephanie A. Murphy / Sandy Shokoples / Anita Wong / Jamil N. Kanji / Graham Tipples

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 9

Abstract: Abstract Omicron has become the dominant SARS-CoV-2 variant globally since December 2021, with distinct waves being associated with separate Omicron sublineages. Rapid detection of BA.1, BA.2, BA.4, and BA.5 was accomplished in the province of Alberta, ... ...

Abstract	Abstract Omicron has become the dominant SARS-CoV-2 variant globally since December 2021, with distinct waves being associated with separate Omicron sublineages. Rapid detection of BA.1, BA.2, BA.4, and BA.5 was accomplished in the province of Alberta, Canada, through the design and implementation of real-time reverse transcriptase PCR assays targeting S:N501Y, S:ins214EPE, S:H69/V70, ORF7b:L11F, and M:D3N. Using the combination of results for each of these markers, samples could be designated as belonging to sublineages within BA.1, BA.2, BA.4, or BA.5. The analytical sensitivity of these markers ranged from 132 to 2229 copies/mL and in-laboratory accuracy was 98.9–100%. A 97.3% agreement using 12,592 specimens was demonstrated for the assays compared to genome sequencing. The use of these assays, combined with genome sequencing, facilitated the surveillance of SARS-CoV-2 lineages throughout a BA.5-dominated period.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of a Free-Living Ketogenic Diet on Feasibility, Satiety, Body Composition, and Metabolic Health in Women: The Grading Level of Optimal Carbohydrate for Women (GLOW) Study.

Saenz, Catherine / Hooper, Stephanie / Orange, Terrance / Knight, Ashlyn / Barragan, Martin / Lynch, Tarah / Remenapp, Abigail / Coyle, Kevin / Winters, Carena / Hausenblas, Heather

Journal of the American College of Nutrition

2021 Volume 40, Issue 4, Page(s) 295–306

Abstract: Objective: The study's purpose was to examine a free-living, ketogenic diet (WFKD) on feasibility, satiety, body composition, and metabolic health in women.: Methods: Twenty-two women (age (yr.) 42.2 ± 8.1, Ht. (cm) 164.2 ± 5.9, BMI 27.3 ± 6.0) ... ...

Abstract	Objective: The study's purpose was to examine a free-living, ketogenic diet (WFKD) on feasibility, satiety, body composition, and metabolic health in women. Methods: Twenty-two women (age (yr.) 42.2 ± 8.1, Ht. (cm) 164.2 ± 5.9, BMI 27.3 ± 6.0) participated in a 21-day, free-living dietary intervention. Daily ketone measurements and satiety/craving surveys, weekly diet records, and PRE and POST assessments of anthropometrics, body composition, blood pressure, and fasted capillary-blood glucose (BG) and cholesterol panels were collected. Results: Women maintained calories (PRE: 1938 kcal vs POST: 1836 kcal) and protein (PRE: 17% vs POST: 20%) but decreased carbohydrate (PRE: 36% vs POST: 13%) and increased fat (PRE: 45% vs POST: 65%) PRE to POST ( Conclusion: Women were able to maintain calories, improve body composition, blood pressure, and BG, increase ketones, and improve some but not all cholesterol markers after 21 days on a free-living WFKD.
MeSH term(s)	Body Composition ; Diet, Ketogenic ; Dietary Carbohydrates ; Feasibility Studies ; Female ; Humans ; Triglycerides
Chemical Substances	Dietary Carbohydrates ; Triglycerides
Language	English
Publishing date	2021-03-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	603204-7
ISSN	1541-1087 ; 0731-5724
ISSN (online)	1541-1087
ISSN	0731-5724
DOI	10.1080/07315724.2021.1875338
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Zs.A 1759: Show issues

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Article: Evolving strategy for an evolving virus: Development of real-time PCR assays for detecting all SARS-CoV-2 variants of concern

Pabbaraju, Kanti / Zelyas, Nathan / Wong, Anita / Croxen, Matthew A. / Lynch, Tarah / Buss, Emily / Murphy, Stephanie / Shokoples, Sandy / Kanji, Jamil / Tipples, Graham

Journal of virological methods. 2022 Sept., v. 307

2022

Abstract: In order to detect the SARS-CoV-2 variants of concern (VOCs), five real-time reverse transcriptase PCR (rRT-PCR) assays were designed to target the critical discriminatory mutations responsible for the following amino acid changes in the spike protein: ... ...

Abstract	In order to detect the SARS-CoV-2 variants of concern (VOCs), five real-time reverse transcriptase PCR (rRT-PCR) assays were designed to target the critical discriminatory mutations responsible for the following amino acid changes in the spike protein: two Δ69–70 + N501Y + E gene triplexes (one optimized for Alpha [B.1.1.7] and one optimized for Omicron [B.1.1.529]), a K417N + 242–244 wild-type duplex, a K417T + E484K duplex, and a L452R + P681 + E484Q triplex. Depending on the assay, sensitivity was 98.97–100% for the detection of known VOC-positive samples, specificity was 97.2–100%, limit of detection was 2–116 copies/reaction, intra- and interassay variability was less than 5%, and no cross-reactivity with common respiratory pathogens was observed with any assay. A subset of rRT-PCR- positive VOC samples were further characterized by genome sequencing. A comparison of the lineage designation by the VOC rRT-PCR assays and genome sequencing for the detection of the Alpha, Beta, Gamma, Delta and Omicron variants showed clinical sensitivities of 99.97–100 %, clinical specificities of 99.6–100 %, positive predictive values of 99.8–100%, and negative predictive values of 99.98–100 %. We have implemented these rRT-PCR assays targeting discriminatory single nucleotide polymorphisms for ongoing VOC screening of SARS-CoV-2 positive samples for surveillance purposes. This has proven extremely useful in providing close to real-time molecular surveillance to monitor the emergence of Alpha, the replacement of Alpha by Delta, and the replacement of Delta by Omicron. While the design, validation and implementation of the variant specific PCR targets is an ever-evolving approach, we find the turn-around-time, high throughput and sensitivity to be a useful complementary approach for SARS-CoV-2 genome sequencing for surveillance purposes in the province of Alberta, Canada.
Keywords	Severe acute respiratory syndrome coronavirus 2 ; amino acids ; cross reaction ; detection limit ; genes ; monitoring ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; viruses ; Alberta
Language	English
Dates of publication	2022-09
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2022.114553
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 1565: Show issues

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Article ; Online: Trends in Population Dynamics of Escherichia coli Sequence Type 131, Calgary, Alberta, Canada, 2006-2016

Peirano, Gisele / Lynch, Tarah / Matsumara, Yasufumi / Nobrega, Diego / Finn, Thomas J / DeVinney, Rebekah / Pitout, Johann D D

Emerging infectious diseases

2020 Volume 26, Issue 12, Page(s) 2907–2915

Abstract: Global expansion of antimicrobial drug-resistant Escherichia coli sequence type (ST) 131 is unrivaled among human bacteria. Understanding trends among ST131 clades will help with designing prevention strategies. We screened E. coli from blood samples (n = ...

Abstract	Global expansion of antimicrobial drug-resistant Escherichia coli sequence type (ST) 131 is unrivaled among human bacteria. Understanding trends among ST131 clades will help with designing prevention strategies. We screened E. coli from blood samples (n = 1,784) obtained in Calgary, Alberta, Canada, during 2006, 2012, and 2016 by PCR for ST131 and positive samples (n = 344) underwent whole-genome sequencing. The incidence rate per 100,000 residents increased from 4.91 during 2006 to 12.35 during 2012 and 10.12 during 2016. ST131 belonged to clades A (10%), B (9%), and C (81%). Clades C1-nonM27 and B were common during 2006, and C2 containing bla
MeSH term(s)	Alberta/epidemiology ; Escherichia coli/genetics ; Escherichia coli Infections/epidemiology ; Humans ; Population Dynamics ; beta-Lactamases/genetics
Chemical Substances	beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2020-12-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid2612.201221
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