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Article ; Online: Community waterborne outbreak linked to a firefighting response during the COVID-19 emergency.

Nascetti, Simona / Busani, Luca / Bartoli, Franco / Orioli, Riccardo / Stenico, Alberta / Regele, Dagmar

Annali dell'Istituto superiore di sanita

2021 Volume 57, Issue 3, Page(s) 226–232

Abstract: Background: On 6 March 2020, a big fire in a village forced the firefighters to draw water simultaneously from many sources, including the Adige river. From 9 March, an increasing number of inhabitants reported gastrointestinal symptoms. We describe the ...

Abstract	Background: On 6 March 2020, a big fire in a village forced the firefighters to draw water simultaneously from many sources, including the Adige river. From 9 March, an increasing number of inhabitants reported gastrointestinal symptoms. We describe the outbreak and the challenges linked to the concurrent COVID-19 spread. Methods: Residents with enteric symptoms and their relatives were interviewed and samples from some of the patients and public water pipelines were tested for enteric pathogens with microbiological and molecular methods. Results: By 20 March, 182 people reported symptoms and 131 met the case definition. Norovirus GI/GII and other pathogens were found in human and water samples. Conclusions: Contamination of the public water network with sewage-contaminated river water through the firefighters pressurized water tank was the suspected source of the outbreak. The investigation was partly hampered due to the SARS-CoV-2 emergency. Control measures included avoiding tap water, alternative water supplies and chlorination of public water.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19/epidemiology ; Child ; Child, Preschool ; Disease Outbreaks ; Emergencies ; Female ; Fires ; Humans ; Italy/epidemiology ; Male ; Middle Aged ; Rivers ; Water Microbiology ; Water Supply ; Young Adult
Language	English
Publishing date	2021-09-23
Publishing country	Italy
Document type	Journal Article
ZDB-ID	950344-4
ISSN	2384-8553 ; 0021-2571
ISSN (online)	2384-8553
ISSN	0021-2571
DOI	10.4415/ANN_21_03_05
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Article ; Online: Determinants of success and sustainability of the WHO multimodal hand hygiene promotion campaign, Italy, 2007-2008 and 2014.

Moro, Maria Luisa / Morsillo, Filomena / Nascetti, Simona / Parenti, Mita / Allegranzi, Benedetta / Pompa, Maria Grazia / Pittet, Didier

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin

2017 Volume 22, Issue 23

Abstract: A national hand hygiene promotion campaign based on the World Health Organization (WHO) multimodal, Clean Care is Safer Care campaign was launched in Italy in 2007. One hundred seventy-five hospitals from 14 of 20 Italian regions participated. Data were ... ...

Abstract	A national hand hygiene promotion campaign based on the World Health Organization (WHO) multimodal, Clean Care is Safer Care campaign was launched in Italy in 2007. One hundred seventy-five hospitals from 14 of 20 Italian regions participated. Data were collected using methods and tools provided by the WHO campaign, translated into Italian. Hand hygiene compliance, ward infrastructure, and healthcare workers' knowledge and perception of healthcare-associated infections and hand hygiene were evaluated before and after campaign implementation. Compliance data from the 65 hospitals returning complete data for all implementation tools were analysed using a multilevel approach. Overall, hand hygiene compliance increased in the 65 hospitals from 40% to 63% (absolute increase: 23%, 95% confidence interval: 22-24%). A wide variation in hand hygiene compliance among wards was observed; inter-ward variability significantly decreased after campaign implementation and the level of perception was the only item associated with this. Long-term sustainability in 48 of these 65 hospitals was assessed in 2014 using the WHO Hand Hygiene Self-Assessment Framework tool. Of the 48 hospitals, 44 scored in the advanced/intermediate categories of hand hygiene implementation progress. The median hand hygiene compliance achieved at the end of the 2007-2008 campaign appeared to be sustained in 2014.
Language	English
Publishing date	2017-06-08
Publishing country	Sweden
Document type	Journal Article
ZDB-ID	1338803-4
ISSN	1560-7917 ; 1025-496X
ISSN (online)	1560-7917
ISSN	1025-496X
DOI	10.2807/1560-7917.ES.2017.22.23.30546
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Article ; Online: Cyanobacteria biennal dynamic in a volcanic mesotrophic lake in central Italy: Strategies to prevent dangerous human exposures to cyanotoxins.

Manganelli, Maura / Stefanelli, Mara / Vichi, Susanna / Andreani, Paolo / Nascetti, Giuseppe / Scialanca, Fabrizio / Scardala, Simona / Testai, Emanuela / Funari, Enzo

Toxicon : official journal of the International Society on Toxinology

2016 Volume 115, Page(s) 28–40

Abstract: Vico Lake, a volcanic meso-eutrophic lake in Central Italy, whose water is used for drinking and recreational activities, experienced the presence of the microcystins (MC) producing cyanobacterium Planktothrix rubescens. In order to assess the human ... ...

Abstract	Vico Lake, a volcanic meso-eutrophic lake in Central Italy, whose water is used for drinking and recreational activities, experienced the presence of the microcystins (MC) producing cyanobacterium Planktothrix rubescens. In order to assess the human health risks and to provide the local health authorities with a scientific basis for planning tailored monitoring activities, we studied P. rubescens ecology and toxicity for two years. P. rubescens generally dominated the phytoplankton community, alternating with Limnothrix redekei, potentially toxic. P. rubescens was distributed throughout the water column during winter; in summer it produced intense blooms where drinking water is collected (-20 m); here MC were detected all year round (0.5-5 μg/L), with implications for drinking water quality. In surface waters, MC posed no risk for recreational activities in summer, while in winter surface blooms and foams (containing up to 56 μg MC/L) can represent a risk for people and children practicing water sports and for animals consuming raw water. Total phosphorus, phosphate and inorganic nitrogen were not relevant to predict densities nor toxicity; however, a strong correlation between P. rubescens density and aminopeptidase ectoenzymatic activity, an enzyme involved in protein degradation, suggested a role of organic nitrogen for this species. The fraction of potentially toxic population, determined both as mcyB(+)/16SrDNA (10-100%) and as the MC/mcyB(+) cells (0.03-0.79 pg MC/cell), was much more variable than usually observed for P. rubescens. Differently from other Italian and European lakes, the correlation between cell density or the mcyB(+) cells and MC explained only ∼50 and 30% of MC variability, respectively: for Vico Lake, monitoring only cell or the mcyB(+) cell density is not sufficient to predict MC concentrations, and consequently to protect population health. Finally, during a winter bloom one site has been sampled weekly, showing that monthly sampling during such a phase could greatly underestimate the 'hazard'. Our results highlight the need to adopt a stepwise monitoring activity, considering the lake and the cyanobacteria specific features. This activity should be complemented with communication to the public and involvement of stakeholders.
MeSH term(s)	Chemical Phenomena ; Cyanobacteria/isolation & purification ; Cyanobacteria/metabolism ; Drinking Water/chemistry ; Drinking Water/microbiology ; Environmental Monitoring ; Humans ; Italy ; Lakes/microbiology ; Microcystins/analysis ; Microcystins/toxicity ; RNA, Ribosomal, 16S/genetics ; RNA, Ribosomal, 16S/isolation & purification ; Seasons
Chemical Substances	Drinking Water ; Microcystins ; RNA, Ribosomal, 16S ; microcystin (77238-39-2)
Language	English
Publishing date	2016-06-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	204479-1
ISSN	1879-3150 ; 0041-0101
ISSN (online)	1879-3150
ISSN	0041-0101
DOI	10.1016/j.toxicon.2016.03.004
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Article ; Online: Laboratory procedures for the diagnosis of tuberculosis: a survey in ten Italian Regions.

Moro, Maria Luisa / Nascetti, Simona / Morsillo, Filomena / Morandi, Matteo

Annali dell'Istituto superiore di sanita

2010 Volume 46, Issue 2, Page(s) 178–184

Abstract: The characteristics of laboratories performing tuberculosis (TB) diagnostic procedures were investigated in ten Italian Regions, through a mailed questionnaire. Three hundred and eighty laboratories answered (70.8 % response rate), 250 of which performed ...

Abstract	The characteristics of laboratories performing tuberculosis (TB) diagnostic procedures were investigated in ten Italian Regions, through a mailed questionnaire. Three hundred and eighty laboratories answered (70.8 % response rate), 250 of which performed directly at least one TB diagnostic procedure. Standard criteria concerning microscopy, culture, identification, and drug susceptibility testing were frequently not satisfied, particularly those related to the volume of activity (32 % of laboratories performing microscopy examined 10 samples and 36 % of those performing culture performed 20 cultures per week), processing time, biosafety requirements and participation to internal/external quality control programs. The survey' results highlight the need to promote the adoption of standardized procedures and to centralize the mycobacteriology testing in a reduced number of high quality laboratories, in order to improve diagnostic accuracy, resource management and quality of surveillance data.
MeSH term(s)	Clinical Laboratory Techniques/standards ; Humans ; Italy ; Surveys and Questionnaires ; Tuberculosis/diagnosis
Language	English
Publishing date	2010-05-27
Publishing country	Italy
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	950344-4
ISSN	2384-8553 ; 0021-2571
ISSN (online)	2384-8553
ISSN	0021-2571
DOI	10.4415/ANN_10_02_12
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Laboratory procedures for the diagnosis of tuberculosis

Maria Luisa Moro / Simona Nascetti / Filomena Morsillo / Matteo Morandi

Annali dell'Istituto Superiore di Sanità, Vol 46, Iss 2, Pp 178-

a survey in ten Italian Regions

2010 Volume 184

Abstract	The characteristics of laboratories performing tuberculosis (TB) diagnostic procedures were investigated in ten Italian Regions, through a mailed questionnaire. Three hundred and eighty laboratories answered (70.8% response rate), 250 of which performed directly at least one TB diagnostic procedure. Standard criteria concerning microscopy, culture, identification, and drug susceptibility testing were frequently not satisfied, particularly those related to the volume of activity (32% of laboratories performing microscopy examined < 10 samples and 36% of those performing culture performed < 20 cultures per week), processing time, biosafety requirements and participation to internal/external quality control programs. The survey's results highlight the need to promote the adoption of standardized procedures and to centralize the mycobacteriology testing in a reduced number of high quality laboratories, in order to improve diagnostic accuracy, resource management and quality of surveillance data.
Keywords	Mycobacterium tuberculosis ; laboratori ; test diagnostici ; indagine ; Medicine ; R ; Public aspects of medicine ; RA1-1270
Publishing date	2010-06-01T00:00:00Z
Publisher	Istituto Superiore di Sanità
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Laboratory procedures for the diagnosis of tuberculosis

Maria Luisa Moro / Simona Nascetti / Filomena Morsillo / Matteo Morandi

Annali dell'Istituto Superiore di Sanità, Vol 46, Iss 2, Pp 178-

a survey in ten Italian Regions

2010 Volume 184

Abstract	The characteristics of laboratories performing tuberculosis (TB) diagnostic procedures were investigated in ten Italian Regions, through a mailed questionnaire. Three hundred and eighty laboratories answered (70.8% response rate), 250 of which performed directly at least one TB diagnostic procedure. Standard criteria concerning microscopy, culture, identification, and drug susceptibility testing were frequently not satisfied, particularly those related to the volume of activity (32% of laboratories performing microscopy examined < 10 samples and 36% of those performing culture performed < 20 cultures per week), processing time, biosafety requirements and participation to internal/external quality control programs. The survey's results highlight the need to promote the adoption of standardized procedures and to centralize the mycobacteriology testing in a reduced number of high quality laboratories, in order to improve diagnostic accuracy, resource management and quality of surveillance data.
Keywords	Mycobacterium tuberculosis ; laboratori ; test diagnostici ; indagine ; Medicine ; R ; Public aspects of medicine ; RA1-1270
Publishing date	2010-06-01T00:00:00Z
Publisher	Istituto Superiore di Sanità
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Laboratory procedures for the diagnosis of tuberculosis

Maria Luisa Moro / Simona Nascetti / Filomena Morsillo / Matteo Morandi

Annali dell'Istituto Superiore di Sanità, Vol 46, Iss 2, Pp 178-

a survey in ten Italian Regions

2010 Volume 184

Abstract	The characteristics of laboratories performing tuberculosis (TB) diagnostic procedures were investigated in ten Italian Regions, through a mailed questionnaire. Three hundred and eighty laboratories answered (70.8% response rate), 250 of which performed directly at least one TB diagnostic procedure. Standard criteria concerning microscopy, culture, identification, and drug susceptibility testing were frequently not satisfied, particularly those related to the volume of activity (32% of laboratories performing microscopy examined < 10 samples and 36% of those performing culture performed < 20 cultures per week), processing time, biosafety requirements and participation to internal/external quality control programs. The survey's results highlight the need to promote the adoption of standardized procedures and to centralize the mycobacteriology testing in a reduced number of high quality laboratories, in order to improve diagnostic accuracy, resource management and quality of surveillance data.
Keywords	Mycobacterium tuberculosis ; laboratori ; test diagnostici ; indagine ; Medicine ; R ; Public aspects of medicine ; RA1-1270
Publishing date	2010-06-01T00:00:00Z
Publisher	Istituto Superiore di Sanità
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Laboratory procedures for the diagnosis of tuberculosis

Maria Luisa Moro / Simona Nascetti / Filomena Morsillo / Matteo Morandi

Annali dell'Istituto Superiore di Sanità, Vol 46, Iss 2, Pp 178-

a survey in ten Italian Regions

2010 Volume 184

Abstract	The characteristics of laboratories performing tuberculosis (TB) diagnostic procedures were investigated in ten Italian Regions, through a mailed questionnaire. Three hundred and eighty laboratories answered (70.8% response rate), 250 of which performed directly at least one TB diagnostic procedure. Standard criteria concerning microscopy, culture, identification, and drug susceptibility testing were frequently not satisfied, particularly those related to the volume of activity (32% of laboratories performing microscopy examined < 10 samples and 36% of those performing culture performed < 20 cultures per week), processing time, biosafety requirements and participation to internal/external quality control programs. The survey's results highlight the need to promote the adoption of standardized procedures and to centralize the mycobacteriology testing in a reduced number of high quality laboratories, in order to improve diagnostic accuracy, resource management and quality of surveillance data.
Keywords	Mycobacterium tuberculosis ; laboratori ; test diagnostici ; indagine ; Medicine ; R ; Public aspects of medicine ; RA1-1270
Publishing date	2010-06-01T00:00:00Z
Publisher	Istituto Superiore di Sanità
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

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Article ; Online: Laboratory procedures for the diagnosis of tuberculosis

Maria Luisa Moro / Simona Nascetti / Filomena Morsillo / Matteo Morandi

Annali dell'Istituto Superiore di Sanità, Vol 46, Iss 2, Pp 178-

a survey in ten Italian Regions

2010 Volume 184

Abstract	The characteristics of laboratories performing tuberculosis (TB) diagnostic procedures were investigated in ten Italian Regions, through a mailed questionnaire. Three hundred and eighty laboratories answered (70.8% response rate), 250 of which performed directly at least one TB diagnostic procedure. Standard criteria concerning microscopy, culture, identification, and drug susceptibility testing were frequently not satisfied, particularly those related to the volume of activity (32% of laboratories performing microscopy examined < 10 samples and 36% of those performing culture performed < 20 cultures per week), processing time, biosafety requirements and participation to internal/external quality control programs. The survey's results highlight the need to promote the adoption of standardized procedures and to centralize the mycobacteriology testing in a reduced number of high quality laboratories, in order to improve diagnostic accuracy, resource management and quality of surveillance data.
Keywords	Mycobacterium tuberculosis ; laboratori ; test diagnostici ; indagine ; Medicine ; R ; Public aspects of medicine ; RA1-1270
Publishing date	2010-06-01T00:00:00Z
Publisher	Istituto Superiore di Sanità
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

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Article ; Online: Laboratory procedures for the diagnosis of tuberculosis

Maria Luisa Moro / Simona Nascetti / Filomena Morsillo / Matteo Morandi

Annali dell'Istituto Superiore di Sanità, Vol 46, Iss 2, Pp 178-

a survey in ten Italian Regions

2010 Volume 184

Abstract	The characteristics of laboratories performing tuberculosis (TB) diagnostic procedures were investigated in ten Italian Regions, through a mailed questionnaire. Three hundred and eighty laboratories answered (70.8% response rate), 250 of which performed directly at least one TB diagnostic procedure. Standard criteria concerning microscopy, culture, identification, and drug susceptibility testing were frequently not satisfied, particularly those related to the volume of activity (32% of laboratories performing microscopy examined < 10 samples and 36% of those performing culture performed < 20 cultures per week), processing time, biosafety requirements and participation to internal/external quality control programs. The survey's results highlight the need to promote the adoption of standardized procedures and to centralize the mycobacteriology testing in a reduced number of high quality laboratories, in order to improve diagnostic accuracy, resource management and quality of surveillance data.
Keywords	Mycobacterium tuberculosis ; laboratori ; test diagnostici ; indagine ; Medicine ; R ; Public aspects of medicine ; RA1-1270
Publishing date	2010-06-01T00:00:00Z
Publisher	Istituto Superiore di Sanità
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

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