LIVIVO - Search results -

Search results

Result 1 - 10 of total 74

Search options

Article: Current Limitations of Staph Infection Diagnostics, and the Role for VOCs in Achieving Culture-Independent Detection.

Jenkins, Carrie L / Bean, Heather D

2023 Volume 12, Issue 2

Abstract: Staphylococci are broadly adaptable and their ability to grow in unique environments has been widely established, but the most common and clinically relevant staphylococcal niche is the skin and mucous membranes of mammals and birds. ...

Abstract	Staphylococci are broadly adaptable and their ability to grow in unique environments has been widely established, but the most common and clinically relevant staphylococcal niche is the skin and mucous membranes of mammals and birds.
Language	English
Publishing date	2023-01-24
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens12020181
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition.

Jenkins, Carrie L / Bean, Heather D

Metabolites

2020 Volume 10, Issue 9

Abstract: In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this ... ...

Abstract	In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of
Language	English
Publishing date	2020-08-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662251-8
ISSN	2218-1989
ISSN	2218-1989
DOI	10.3390/metabo10090347
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Volatile Metabolites in Lavage Fluid Are Correlated with Cytokine Production in a Valley Fever Murine Model.

Higgins Keppler, Emily A / Van Dyke, Marley C Caballero / Mead, Heather L / Lake, Douglas F / Magee, D Mitchell / Barker, Bridget M / Bean, Heather D

Journal of fungi (Basel, Switzerland)

2023 Volume 9, Issue 1

Abstract: Coccidioides immitis and Coccidioides posadasii are soil-dwelling fungi of arid regions in North and South America that are responsible for Valley fever (coccidioidomycosis). Forty percent of patients with Valley fever exhibit symptoms ranging from mild, ...

Abstract	Coccidioides immitis and Coccidioides posadasii are soil-dwelling fungi of arid regions in North and South America that are responsible for Valley fever (coccidioidomycosis). Forty percent of patients with Valley fever exhibit symptoms ranging from mild, self-limiting respiratory infections to severe, life-threatening pneumonia that requires treatment. Misdiagnosis as bacterial pneumonia commonly occurs in symptomatic Valley fever cases, resulting in inappropriate treatment with antibiotics, increased medical costs, and delay in diagnosis. In this proof-of-concept study, we explored the feasibility of developing breath-based diagnostics for Valley fever using a murine lung infection model. To investigate potential volatile biomarkers of Valley fever that arise from host−pathogen interactions, we infected C57BL/6J mice with C. immitis RS (n = 6), C. posadasii Silveira (n = 6), or phosphate-buffered saline (n = 4) via intranasal inoculation. We measured fungal dissemination and collected bronchoalveolar lavage fluid (BALF) for cytokine profiling and for untargeted volatile metabolomics via solid-phase microextraction (SPME) and two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC×GC-TOFMS). We identified 36 volatile organic compounds (VOCs) that were significantly correlated (p < 0.05) with cytokine abundance. These 36 VOCs clustered mice by their cytokine production and were also able to separate mice with moderate-to-high cytokine production by infection strain. The data presented here show that Coccidioides and/or the host produce volatile metabolites that may yield biomarkers for a Valley fever breath test that can detect coccidioidal infection and provide clinically relevant information on primary pulmonary disease severity.
Language	English
Publishing date	2023-01-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2784229-0
ISSN	2309-608X ; 2309-608X
ISSN (online)	2309-608X
ISSN	2309-608X
DOI	10.3390/jof9010115
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Life Cycle Dominates the Volatilome Character of Dimorphic Fungus

Higgins Keppler, Emily A / Mead, Heather L / Barker, Bridget M / Bean, Heather D

mSphere

2021 Volume 6, Issue 2

Abstract: Valley fever (coccidioidomycosis) is an endemic fungal pneumonia of the North and South American deserts. The causative agents of Valley fever are the dimorphic ... ...

Abstract	Valley fever (coccidioidomycosis) is an endemic fungal pneumonia of the North and South American deserts. The causative agents of Valley fever are the dimorphic fungi
MeSH term(s)	Biomarkers/metabolism ; Breath Tests/methods ; Coccidioides/classification ; Coccidioides/growth & development ; Coccidioides/metabolism ; Coccidioidomycosis/microbiology ; Culture Media/chemistry ; Humans ; Life Cycle Stages ; Metabolome ; Mycelium/growth & development ; Mycelium/metabolism ; Volatile Organic Compounds/analysis
Chemical Substances	Biomarkers ; Culture Media ; Volatile Organic Compounds
Language	English
Publishing date	2021-04-14
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/mSphere.00040-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Influence of media on the differentiation of Staphylococcus spp. by volatile compounds.

Jenkins, Carrie L / Bean, Heather D

Journal of breath research

2019 Volume 14, Issue 1, Page(s) 16007

Abstract: Staphylococcus aureus asymptomatically colonizes a third of the world's population, and it is an opportunistic pathogen that can cause life threatening diseases. To diagnose S. aureus infections, it is necessary to differentiate S. aureus from the ... ...

Abstract	Staphylococcus aureus asymptomatically colonizes a third of the world's population, and it is an opportunistic pathogen that can cause life threatening diseases. To diagnose S. aureus infections, it is necessary to differentiate S. aureus from the ubiquitous human commensal Staphylococcus epidermidis, which beneficially colonizes the skin of all humans. Efforts are underway to identify volatile biomarkers for diagnosing S. aureus infections, but to date no studies have investigated whether S. aureus and S. epidermidis can be reliably differentiated under a variety of growth conditions. The overall goal of this study was to evaluate the influence of growth medium on the ability to differentiate S. aureus and S. epidermidis based on their volatile profiles. We used headspace solid-phase microextraction (HS-SPME) and comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC×GC-TOFMS) to examine the headspace volatiles of S. aureus and S. epidermidis when aerobically grown in four different complex media. We detected 337 volatile features when culturing S. aureus and S. epidermidis in four complex media, termed the staph volatiles, and found only 20%-40% concurrence in the volatiles produced by these two species in any single medium. Using principal components analysis and hierarchical clustering analysis on the staph volatiles, we observed that S. aureus and S. epidermidis clustered independently from each other, and distinctly clustered by growth medium within species. Removing volatiles that are species and/or media-specific from the analysis reduced the resolution between species clusters, but in all models clustering by species overrode clustering by media type. These analyses suggest that, while volatile profiles are media-specific, species differences dominate the staph volatilome. These data enable future investigations into the identification of volatile biomarkers to discriminate staphylococcal pathogens versus commensals, which will improve staph diagnoses and provide insights into the biochemistry of staph infections and immunity.
MeSH term(s)	Biomarkers/analysis ; Breath Tests ; Cluster Analysis ; Culture Media/chemistry ; Gas Chromatography-Mass Spectrometry ; Humans ; Principal Component Analysis ; Solid Phase Microextraction ; Staphylococcus aureus/growth & development ; Staphylococcus aureus/isolation & purification ; Staphylococcus epidermidis/growth & development ; Staphylococcus epidermidis/isolation & purification ; Volatile Organic Compounds/analysis
Chemical Substances	Biomarkers ; Culture Media ; Volatile Organic Compounds
Language	English
Publishing date	2019-11-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2381007-5
ISSN	1752-7163 ; 1752-7155
ISSN (online)	1752-7163
ISSN	1752-7155
DOI	10.1088/1752-7163/ab3e9d
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Addressing Missing Data in GC × GC Metabolomics: Identifying Missingness Type and Evaluating the Impact of Imputation Methods on Experimental Replication.

Davis, Trenton J / Firzli, Tarek R / Higgins Keppler, Emily A / Richardson, Matthew / Bean, Heather D

Analytical chemistry

2022 Volume 94, Issue 31, Page(s) 10912–10920

Abstract: Missing data is a significant issue in metabolomics that is often neglected when conducting data preprocessing, particularly when it comes to imputation. This can have serious implications for downstream statistical analyses and lead to misleading or ... ...

Abstract	Missing data is a significant issue in metabolomics that is often neglected when conducting data preprocessing, particularly when it comes to imputation. This can have serious implications for downstream statistical analyses and lead to misleading or uninterpretable inferences. In this study, we aim to identify the primary types of missingness that affect untargeted metabolomics data and compare strategies for imputation using two real-world comprehensive two-dimensional gas chromatography (GC × GC) data sets. We also present these goals in the context of experimental replication whereby imputation is conducted in a within-replicate-based fashion─the first description and evaluation of this strategy─and introduce an R package
MeSH term(s)	Bayes Theorem ; Chromatography, Gas ; Metabolomics/methods ; Principal Component Analysis ; Reproducibility of Results
Language	English
Publishing date	2022-07-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.1c04093
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4033: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: A Metabolomic Approach for Predicting Diurnal Changes in Cortisol.

Eshima, Jarrett / Davis, Trenton J / Bean, Heather D / Fricks, John / Smith, Barbara S

Metabolites

2020 Volume 10, Issue 5

Abstract: ... ...

Abstract	Introduction
Language	English
Publishing date	2020-05-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662251-8
ISSN	2218-1989
ISSN	2218-1989
DOI	10.3390/metabo10050194
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Inhibition of Fungal Growth and Induction of a Novel Volatilome in Response to

Ebadzadsahrai, Ghazal / Higgins Keppler, Emily A / Soby, Scott D / Bean, Heather D

Frontiers in microbiology

2020 Volume 11, Page(s) 1035

Abstract: The study of chemical bioactivity in the rhizosphere has recently broadened to include microbial metabolites, and their roles in niche construction and competition via growth promotion, growth inhibition, and toxicity. Several prior studies have ... ...

Abstract	The study of chemical bioactivity in the rhizosphere has recently broadened to include microbial metabolites, and their roles in niche construction and competition via growth promotion, growth inhibition, and toxicity. Several prior studies have identified bacteria that produce volatile organic compounds (VOCs) with antifungal activities, indicating their potential use as biocontrol organisms to suppress phytopathogenic fungi and reduce agricultural losses. We sought to expand the roster of soil bacteria with known antifungal VOCs by testing bacterial isolates from wild and cultivated cranberry bog soils for VOCs that inhibit the growth of four common fungal and oomycete plant pathogens, and
Language	English
Publishing date	2020-05-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2020.01035
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Addressing Missing Data in GC × GC Metabolomics: Identifying Missingness Type and Evaluating the Impact of Imputation Methods on Experimental Replication

Davis, Trenton J. / Firzli, Tarek R. / Higgins Keppler, Emily A. / Richardson, Matthew / Bean, Heather D.

Analytical Chemistry. 2022 July 26, v. 94, no. 31 p.10912-10920

2022

Abstract	Missing data is a significant issue in metabolomics that is often neglected when conducting data preprocessing, particularly when it comes to imputation. This can have serious implications for downstream statistical analyses and lead to misleading or uninterpretable inferences. In this study, we aim to identify the primary types of missingness that affect untargeted metabolomics data and compare strategies for imputation using two real-world comprehensive two-dimensional gas chromatography (GC × GC) data sets. We also present these goals in the context of experimental replication whereby imputation is conducted in a within-replicate-based fashion—the first description and evaluation of this strategy—and introduce an R package MetabImpute to carry out these analyses. Our results conclude that, in these two GC × GC data sets, missingness was most likely of the missing at-random (MAR) and missing not-at-random (MNAR) types as opposed to missing completely at-random (MCAR). Gibbs sampler imputation and Random Forest gave the best results when imputing MAR and MNAR compared against single-value imputation (zero, minimum, mean, median, and half-minimum) and other more sophisticated approaches (Bayesian principal component analysis and quantile regression imputation for left-censored data). When samples are replicated, within-replicate imputation approaches led to an increase in the reproducibility of peak quantification compared to imputation that ignores replication, suggesting that imputing with respect to replication may preserve potentially important features in downstream analyses for biomarker discovery.
Keywords	Bayesian theory ; analytical chemistry ; biomarkers ; comprehensive two-dimensional gas chromatography ; metabolomics ; principal component analysis ; regression analysis
Language	English
Dates of publication	2022-0726
Size	p. 10912-10920.
Publishing place	American Chemical Society
Document type	Article ; Online
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.1c04093
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 4' 52/94: Show issues
Z 2.9: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Pseudomonas aeruginosa Volatilome Characteristics and Adaptations in Chronic Cystic Fibrosis Lung Infections.

Davis, Trenton J / Karanjia, Ava V / Bhebhe, Charity N / West, Sarah B / Richardson, Matthew / Bean, Heather D

mSphere

2020 Volume 5, Issue 5

Abstract: Pseudomonas ... ...

Abstract	Pseudomonas aeruginosa
MeSH term(s)	Adaptation, Physiological ; Biomarkers/analysis ; Chromatography, Gas ; Chronic Disease ; Cystic Fibrosis/microbiology ; Humans ; Lung/microbiology ; Mass Spectrometry ; Metabolome ; Phenotype ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/metabolism ; Quality of Life ; Respiratory Tract Infections/microbiology ; Volatile Organic Compounds/analysis ; Volatilization
Chemical Substances	Biomarkers ; Volatile Organic Compounds
Language	English
Publishing date	2020-10-07
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/mSphere.00843-20
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED