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Article ; Online: Serum concentrations of IL-16 and its genetic polymorphism rs4778889 affect the susceptibility and severity of endometriosis in Nigerian women.

Babah, Ochuwa Adiketu / Ojewunmi, Oyesola Oyewole / Onwuamah, Chika Kingsley / Udenze, Ifeoma Christiana / Osuntoki, Akinniyi Adediran / Afolabi, Bosede Bukola

BMC women's health

2023 Volume 23, Issue 1, Page(s) 253

Abstract: Background: Endometriosis is the presence of active ectopic endometrial glands and stroma at other sites outside the uterine cavity. It is a common cause of chronic pelvic pain which is sometimes debilitating, and inflammation is one of the known ... ...

Abstract	Background: Endometriosis is the presence of active ectopic endometrial glands and stroma at other sites outside the uterine cavity. It is a common cause of chronic pelvic pain which is sometimes debilitating, and inflammation is one of the known triggers of endometriosis. Interleukins 6 and 16 (IL-6 and IL-16) are proinflammatory cytokines which play essential roles in inflammatory diseases. We therefore investigated the relationship between genetic polymorphisms of interleukins 6 and 16, and the development of endometriosis in Nigerian women. Method: One hundred and thirty (130) consenting women were consecutively enrolled, sixty-five (65) of whom had endometriosis and 65 age-matched women as reference group, surgically confirmed as not having endometriosis. Spectrophotometric determination of serum concentrations of Interleukins 6 and 16 was carried out and the genotyping of IL-6 (rs1800795) and IL-16 (rs4778889, rs11556218, rs4072111) genes were performed using TaqMan assays. Results: Serum IL-16 concentration was significantly higher in women with severe chronic pelvic pain compared to those with mild pain (p = 0.023). The C allele of rs4778889 was associated with endometriosis (OR: 1.80, 95% CI: 1.08 - 3.02, p = 0.024). Conclusion: Serum IL-16 and IL-16 rs4778889 may be important markers for endometriosis in Nigerian, and by extension, African women. Multicentre African studies would clarify this.
MeSH term(s)	Humans ; Female ; Endometriosis/genetics ; Endometriosis/complications ; Interleukin-16/genetics ; Genetic Predisposition to Disease ; Interleukin-6/genetics ; Polymorphism, Single Nucleotide ; Pelvic Pain/genetics ; Pelvic Pain/complications ; Chronic Pain/complications ; Case-Control Studies
Chemical Substances	Interleukin-16 ; Interleukin-6
Language	English
Publishing date	2023-05-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2050444-5
ISSN	1472-6874 ; 1472-6874
ISSN (online)	1472-6874
ISSN	1472-6874
DOI	10.1186/s12905-023-02362-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Mechanism of Viral Suppression among HIV Elite Controllers and Long-Term Nonprogressors in Nigeria and South Africa.

Ahmed, Rahaman Ademolu / Adekoya, Khalid Olajide / Onwuamah, Chika Kingsley / Oboh, Bolanle Olufunmilayo / Iyer, Smita Swaminathan / Oluwatosin, Ayomide Samuel / Audu, Rosemary Ajuma / Ezechi, Oliver Chukwujekwu

Viruses

2022 Volume 14, Issue 6

Abstract: A subgroup among people living with HIV (PLHIV) experience viral suppression, sometimes to an undetectable level in the blood and/or are able to maintain a healthy CD4+ T-cell count without the influence of antiretroviral (ARV) therapy. One out of three ... ...

Abstract	A subgroup among people living with HIV (PLHIV) experience viral suppression, sometimes to an undetectable level in the blood and/or are able to maintain a healthy CD4+ T-cell count without the influence of antiretroviral (ARV) therapy. One out of three hundred PLHIV fall into this category, and a large sample of this group can be found in areas with a high prevalence of HIV infection such as Nigeria and South Africa. Understanding the mechanism underpinning the nonprogressive phenotype in this subgroup may provide insights into the control of the global HIV epidemic. This work provides mechanisms of the elite control and nonprogressive phenotype among PLHIV in Nigeria and South Africa and identifies research gaps that will contribute to a better understanding on HIV controllers among PLHIV.
MeSH term(s)	Elite Controllers ; HIV Infections/drug therapy ; HIV Infections/genetics ; Humans ; Nigeria/epidemiology ; Prevalence ; South Africa/epidemiology ; Viral Load
Language	English
Publishing date	2022-06-10
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, N.I.H., Extramural
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v14061270
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Article: Mechanism of Viral Suppression among HIV Elite Controllers and Long-Term Nonprogressors in Nigeria and South Africa

Viruses. 2022 June 10, v. 14, no. 6

2022

Abstract	A subgroup among people living with HIV (PLHIV) experience viral suppression, sometimes to an undetectable level in the blood and/or are able to maintain a healthy CD4+ T-cell count without the influence of antiretroviral (ARV) therapy. One out of three hundred PLHIV fall into this category, and a large sample of this group can be found in areas with a high prevalence of HIV infection such as Nigeria and South Africa. Understanding the mechanism underpinning the nonprogressive phenotype in this subgroup may provide insights into the control of the global HIV epidemic. This work provides mechanisms of the elite control and nonprogressive phenotype among PLHIV in Nigeria and South Africa and identifies research gaps that will contribute to a better understanding on HIV controllers among PLHIV.
Keywords	CD4-positive T-lymphocytes ; HIV infections ; HIV long-term survivors ; antiretroviral agents ; phenotype ; therapeutics ; Nigeria ; South Africa
Language	English
Dates of publication	2022-0610
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v14061270
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Low level SARS-CoV-2 RNA detected in plasma samples from a cohort of Nigerians: Implications for blood transfusion.

Okwuraiwe, Azuka Patrick / Onwuamah, Chika Kingsley / Shaibu, Joseph Ojonugwa / Amoo, Samuel Olufemi / Ige, Fehintola Anthonia / James, Ayorinde Babatunde / Okoli, Leona Chika / Ahmed, Abul-Rahman / Ponmak, Jamda / Sokei, Judith O / Akanmu, Sulaimon / Salako, Babatunde Lawal / Audu, Rosemary Ajuma

PloS one

2021 Volume 16, Issue 6, Page(s) e0252611

Abstract: The present global pandemic triggered by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has lingered for over a year in its devastating effects. Diagnosis of coronavirus disease 2019 (COVID-19) is currently established with a ... ...

Abstract	The present global pandemic triggered by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has lingered for over a year in its devastating effects. Diagnosis of coronavirus disease 2019 (COVID-19) is currently established with a polymerase chain reaction (PCR) test by means of oropharyngeal-, nasopharyngeal-, anal-swabs, sputum and blood plasma. However, oral and nasal swabs are more commonly used. This study, therefore, assessed sensitivity and specificity of plasma as a diagnostic in comparison with a combination of oral and nasal swab samples, and the implications for blood transfusion. Oropharyngeal (OP) and nasopharyngeal (NP) swab samples were obtained from 125 individuals suspected to have COVID-19 and stored in viral transport medium (VTM) tubes. Ten millilitres of blood samples in EDTA were also obtained by venepuncture and spun to obtain plasma. Viral RNA was obtained from both swabs and plasma by manual extraction with Qiagen QIAamp viral RNA Mini Kit. Detection was done using a real time fluorescent RT-qPCR BGI kit, on a QuantStudio 3 real-time PCR instrument. Average age of study participants was 41 years, with 74 (59.2%) being male. Out of the 125 individuals tested for COVID-19, 75 (60%) were positive by OP/NP swab. However, only 6 (4.8%) had a positive plasma result for COVID-19 with median Ct value of 32.4. Sensitivity and specificity of RT-PCR SARS-CoV-2 test using plasma was 8% and 100% respectively. There was no false positive recorded, but 69 (55.2%) false negatives were obtained by plasma. SARS-CoV-2 viral RNA was detected, albeit low (4.8%) in plasma. Plasma is likely not a suitable biological sample to diagnose acute SARS-CoV-2 infection. The implication of transfusing blood in this era of COVID-19 needs further investigations.
MeSH term(s)	Adolescent ; Adult ; Aged ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19 Nucleic Acid Testing ; Cross-Sectional Studies ; Female ; Humans ; Male ; Middle Aged ; Nigeria/epidemiology ; RNA, Viral/analysis ; RNA, Viral/blood ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Young Adult
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-06-10
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0252611
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Article: Prevalence and risk factors for high-risk human papillomavirus infection among women from three southern geopolitical zones of Nigeria.

Onwuamah, Chika Kingsley / Feng, Ning / Momoh, Abidemi Esther / Uwandu, Mabel / Ahmed, Rahaman Ademolu / Idigbe, Ifeoma / Vincent, Grace Deborah / Ogbu, Chinenye Angela / Okonkwo, Nkem / Sokei, Judith / Abimbola, Bowofoluwa Sharon / Ojopagogo, Temiloluwa / Okoli, Leona Chika / Adesina, Mary / Ezemelue, Priscilla Ngozi / Sowunmi, Omowunmi / Okwuzu, Jane / Labo Popoola, Olaoniye Habeebat / Shaibu, Joseph Ojonugwa /

Ohihoin, Greg Aigbe / Nzeribe, Emily / David, Agatha / Olaleye, Olufemi / Ofotokun, Ighovwerha / Dong, Xiao Ping / Ezechi, Oliver Chukwujekwu

Frontiers in oncology

2023 Volume 13, Page(s) 1254304

Abstract: Introduction: Human Papillomavirus (HPV) infection is a risk factor for cervical cancer, the fourth most common cancer among women globally. Its burden is the highest in sub-Saharan Africa, with over 90% mortality. Interventions may fail without ... ...

Abstract	Introduction: Human Papillomavirus (HPV) infection is a risk factor for cervical cancer, the fourth most common cancer among women globally. Its burden is the highest in sub-Saharan Africa, with over 90% mortality. Interventions may fail without evidence-based data on stratified prevalence and risk factors among most at-risk women across Nigeria. Methods: A cross-sectional comparative study, with participants recruited from the Nigerian Institute of Medical Research's Clinics, NGO outreaches, a cancer screening centre and a university teaching hospital. Questionnaires were self-administered. Trained medics performed sampling at healthcare facilities, and self-sampling was used at outreaches. Results: Nine hundred eighty-five study participants were recruited. About 37% and 27% of the women knew about HPV and its vaccines, respectively, but only 6% confirmed vaccination with HPV vaccines. HPV prevalence was highest among women with unknown marital status (35.9%), single women (33.8%), widowed/divorced/separated women (30.3%), and married/cohabiting women (19.6%). HPV infection was significantly higher among women who take alcohol (odds=1.7 [95% CI: 1.2-2.4]) and women who smoke (odds=2.6 [95% CI: 1.4 - 4.6]. HPV strains detected included HPV16 (1.3%), HPV18 (1.5%), Low Risk (0.2%) and Other High-Risk groups (19.7%). Conclusion: The inverse relationship between prevalence and education suggests interventions improving awareness and prevention would be impactful. Such interventions could also target HIV-positive women, women presenting with sexually-transmitted infections, who smoke and frequently drink alcohol.
Language	English
Publishing date	2023-10-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2023.1254304
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Article ; Online: SARS-CoV-2 sequencing collaboration in west Africa shows best practices.

Onwuamah, Chika Kingsley / Kanteh, Abdoulie / Abimbola, Bowofoluwa Sharon / Ahmed, Rahaman Ademolu / Okoli, Chika Leona / Shaibu, Joseph Ojonugwa / James, Ayorinde B / Ajibaye, Olusola / Okwuraiwe, Azuka P / Fowora, Muinah / Otuonye, Ngozi / Worwui, Archibald / Iwalokun, Bamidele / Kanteh, Dembo / Audu, Rosemary A / Adegbola, Richard Adebayo / D'Alessandro, Umberto / Salako, Babatunde Lawal / Sesay, Abdul Karim

The Lancet. Global health

2021 Volume 9, Issue 11, Page(s) e1499–e1500

MeSH term(s)	Africa, Western/epidemiology ; COVID-19/epidemiology ; COVID-19/virology ; Cooperative Behavior ; Humans ; Interinstitutional Relations ; SARS-CoV-2/genetics ; Whole Genome Sequencing
Language	English
Publishing date	2021-09-22
Publishing country	England
Document type	Letter
ZDB-ID	2723488-5
ISSN	2214-109X ; 2214-109X
ISSN (online)	2214-109X
ISSN	2214-109X
DOI	10.1016/S2214-109X(21)00389-2
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Article ; Online: SARS-CoV-2 sequencing collaboration in west Africa shows best practices

Chika Kingsley Onwuamah / Abdoulie Kanteh / Bowofoluwa Sharon Abimbola / Rahaman Ademolu Ahmed / Chika Leona Okoli / Joseph Ojonugwa Shaibu / Ayorinde B James / Olusola Ajibaye / Azuka P Okwuraiwe / Muinah Fowora / Ngozi Otuonye / Archibald Worwui / Bamidele Iwalokun / Dembo Kanteh / Rosemary A Audu / Richard Adebayo Adegbola / Umberto D'Alessandro / Babatunde Lawal Salako / Abdul Karim Sesay

The Lancet Global Health, Vol 9, Iss 11, Pp e1499-e

2021 Volume 1500

Keywords	Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2021-11-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Self-collected versus medic-collected sampling for human papillomavirus testing among women in Lagos, Nigeria: a comparative study.

BMC public health

2022 Volume 22, Issue 1, Page(s) 1922

Abstract: Objective: To evaluate the feasibility and performance of self-collected vaginal swab samples for HPV screening among women in Lagos, Nigeria.: Methods: A cross-sectional study was implemented from March to August 2020 among sexually active women. ... ...

Abstract	Objective: To evaluate the feasibility and performance of self-collected vaginal swab samples for HPV screening among women in Lagos, Nigeria. Methods: A cross-sectional study was implemented from March to August 2020 among sexually active women. Study participants provided same-day paired vaginal swab samples. Medic-sampling and poster-directed self-sampling methods were used to collect the two samples per participant. A real-time PCR assay detected HPV 16, HPV 18, other-high-risk (OHR) HPV, and the human β-globin gene. The self-collected samples' sensitivity, specificity, and accuracy were determined against the medic-collected samples using the MedCalc Online Diagnostic Calculator. Results: Of the 213 women aged 16 ~ 63-year-old recruited, 187 (88%) participants had concordant results, while 26 (12%) participants had discordant results. Among the 187 concordant results, 35 (19%) were HPV positive, 150 (80%) participants were HPV negative, and two (1%) were invalid. 18 (69%) out of the 26 discordant samples were invalid. The self-collected sample was invalid for 14 (54%) participants. Two (8%) medic-collected samples were invalid. Compared to the medic-collected sample, the self-collected sample was 89.80% (95% CI: 77.77 ~ 96.60%) sensitive and 98.21% (95% CI: 94.87 ~ 99.63%) specific, with an accuracy of 96.31% (95% CI: 92.87 ~ 98.40%). The mean age for HPV positive and negative participants were 39 and 40, respectively, with an ANOVA p-value of 0.3932. The stratification of HPV infection by the age group was not statistically significant (P > 0.05). Conclusions: With high accuracy of 96%, self-collected sampling is adequate when tested with real-time PCR and may increase the uptake of HPV testing. Though more self-collected samples were invalid than medic-collected samples, most likely due to poor collection, they could be identified for repeat testing. Future implementation can avoid this error with improved guidance and awareness.
MeSH term(s)	Alphapapillomavirus ; Cross-Sectional Studies ; Early Detection of Cancer/methods ; Female ; Humans ; Middle Aged ; Nigeria ; Papillomaviridae/genetics ; Papillomavirus Infections/diagnosis ; Sensitivity and Specificity ; Specimen Handling/methods ; Uterine Cervical Neoplasms/diagnosis ; Vaginal Smears/methods ; beta-Globins
Chemical Substances	beta-Globins
Language	English
Publishing date	2022-10-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2041338-5
ISSN	1471-2458 ; 1471-2458
ISSN (online)	1471-2458
ISSN	1471-2458
DOI	10.1186/s12889-022-14222-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Low level SARS-CoV-2 RNA detected in plasma samples from a cohort of Nigerians

Azuka Patrick Okwuraiwe / Chika Kingsley Onwuamah / Joseph Ojonugwa Shaibu / Samuel Olufemi Amoo / Fehintola Anthonia Ige / Ayorinde Babatunde James / Leona Chika Okoli / Abul-Rahman Ahmed / Jamda Ponmak / Judith O Sokei / Sulaimon Akanmu / Babatunde Lawal Salako / Rosemary Ajuma Audu

PLoS ONE, Vol 16, Iss 6, p e

Implications for blood transfusion.

2021 Volume 0252611

Abstract	The present global pandemic triggered by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has lingered for over a year in its devastating effects. Diagnosis of coronavirus disease 2019 (COVID-19) is currently established with a polymerase chain reaction (PCR) test by means of oropharyngeal-, nasopharyngeal-, anal-swabs, sputum and blood plasma. However, oral and nasal swabs are more commonly used. This study, therefore, assessed sensitivity and specificity of plasma as a diagnostic in comparison with a combination of oral and nasal swab samples, and the implications for blood transfusion. Oropharyngeal (OP) and nasopharyngeal (NP) swab samples were obtained from 125 individuals suspected to have COVID-19 and stored in viral transport medium (VTM) tubes. Ten millilitres of blood samples in EDTA were also obtained by venepuncture and spun to obtain plasma. Viral RNA was obtained from both swabs and plasma by manual extraction with Qiagen QIAamp viral RNA Mini Kit. Detection was done using a real time fluorescent RT-qPCR BGI kit, on a QuantStudio 3 real-time PCR instrument. Average age of study participants was 41 years, with 74 (59.2%) being male. Out of the 125 individuals tested for COVID-19, 75 (60%) were positive by OP/NP swab. However, only 6 (4.8%) had a positive plasma result for COVID-19 with median Ct value of 32.4. Sensitivity and specificity of RT-PCR SARS-CoV-2 test using plasma was 8% and 100% respectively. There was no false positive recorded, but 69 (55.2%) false negatives were obtained by plasma. SARS-CoV-2 viral RNA was detected, albeit low (4.8%) in plasma. Plasma is likely not a suitable biological sample to diagnose acute SARS-CoV-2 infection. The implication of transfusing blood in this era of COVID-19 needs further investigations.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Low level SARS-CoV-2 RNA detected in plasma samples from a cohort of Nigerians

Azuka Patrick Okwuraiwe / Chika Kingsley Onwuamah / Joseph Ojonugwa Shaibu / Samuel Olufemi Amoo / Fehintola Anthonia Ige / Ayorinde Babatunde James / Leona Chika Okoli / Abul-Rahman Ahmed / Jamda Ponmak / Judith O. Sokei / Sulaimon Akanmu / Babatunde Lawal Salako / Rosemary Ajuma Audu

PLoS ONE, Vol 16, Iss

Implications for blood transfusion

2021 Volume 6

Abstract	The present global pandemic triggered by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has lingered for over a year in its devastating effects. Diagnosis of coronavirus disease 2019 (COVID-19) is currently established with a polymerase chain reaction (PCR) test by means of oropharyngeal-, nasopharyngeal-, anal-swabs, sputum and blood plasma. However, oral and nasal swabs are more commonly used. This study, therefore, assessed sensitivity and specificity of plasma as a diagnostic in comparison with a combination of oral and nasal swab samples, and the implications for blood transfusion. Oropharyngeal (OP) and nasopharyngeal (NP) swab samples were obtained from 125 individuals suspected to have COVID-19 and stored in viral transport medium (VTM) tubes. Ten millilitres of blood samples in EDTA were also obtained by venepuncture and spun to obtain plasma. Viral RNA was obtained from both swabs and plasma by manual extraction with Qiagen QIAamp viral RNA Mini Kit. Detection was done using a real time fluorescent RT-qPCR BGI kit, on a QuantStudio 3 real-time PCR instrument. Average age of study participants was 41 years, with 74 (59.2%) being male. Out of the 125 individuals tested for COVID-19, 75 (60%) were positive by OP/NP swab. However, only 6 (4.8%) had a positive plasma result for COVID-19 with median Ct value of 32.4. Sensitivity and specificity of RT-PCR SARS-CoV-2 test using plasma was 8% and 100% respectively. There was no false positive recorded, but 69 (55.2%) false negatives were obtained by plasma. SARS-CoV-2 viral RNA was detected, albeit low (4.8%) in plasma. Plasma is likely not a suitable biological sample to diagnose acute SARS-CoV-2 infection. The implication of transfusing blood in this era of COVID-19 needs further investigations.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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