LIVIVO - Search results -

Search results

Result 1 - 10 of total 17

Search options

Article ; Online: Accumulation of tissue-resident natural killer cells, innate lymphoid cells, and CD8

Brownlie, Demi / von Kries, Andreas / Valenzano, Giampiero / Wild, Nicole / Yilmaz, Emel / Säfholm, Jesper / Al-Ameri, Mamdoh / Alici, Evren / Ljunggren, Hans-Gustaf / Schliemann, Igor / Aricak, Ozan / Haglund de Flon, Felix / Michaëlsson, Jakob / Marquardt, Nicole

Oncoimmunology

2023 Volume 12, Issue 1, Page(s) 2233402

Abstract: Lung cancer is a leading cause of cancer-related death worldwide. Despite recent advances in tissue immunology, little is known about the spatial distribution of tissue-resident lymphocyte subsets in lung tumors. Using high-parameter flow cytometry, we ... ...

Abstract	Lung cancer is a leading cause of cancer-related death worldwide. Despite recent advances in tissue immunology, little is known about the spatial distribution of tissue-resident lymphocyte subsets in lung tumors. Using high-parameter flow cytometry, we identified an accumulation of tissue-resident lymphocytes including tissue-resident NK (trNK) cells and CD8
MeSH term(s)	Humans ; CD8-Positive T-Lymphocytes ; Immunity, Innate ; Integrin alpha1/metabolism ; Lung Neoplasms ; Killer Cells, Natural/metabolism ; Carcinoma, Non-Small-Cell Lung
Chemical Substances	Integrin alpha1
Language	English
Publishing date	2023-07-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	2645309-5
ISSN	2162-402X ; 2162-402X
ISSN (online)	2162-402X
ISSN	2162-402X
DOI	10.1080/2162402X.2023.2233402
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor β.

Brownlie, Demi / Doughty-Shenton, Dahlia / Yh Soong, Daniel / Nixon, Colin / O Carragher, Neil / M Carlin, Leo / Kitamura, Takanori

Journal for immunotherapy of cancer

2021 Volume 9, Issue 1

Abstract: Background: Metastatic breast cancer is a leading cause of cancer-related death in women worldwide. Infusion of natural killer (NK) cells is an emerging immunotherapy for such malignant tumors, although elimination of the immunosuppressive tumor ... ...

Abstract	Background: Metastatic breast cancer is a leading cause of cancer-related death in women worldwide. Infusion of natural killer (NK) cells is an emerging immunotherapy for such malignant tumors, although elimination of the immunosuppressive tumor environment is required to improve its efficacy. The effects of this "metastatic" tumor environment on NK cells, however, remain largely unknown. Previous studies, including our own, have demonstrated that metastasis-associated macrophages (MAMs) are one of the most abundant immune cell types in the metastatic tumor niche in mouse models of metastatic breast cancer. We thus investigated the effects of MAMs on antitumor functions of NK cells in the metastatic tumor microenvironment. Methods: MAMs were isolated from the tumor-bearing lung of C57BL/6 mice intravenously injected with E0771-LG mouse mammary tumor cells. The effects of MAMs on NK cell cytotoxicity towards E0771-LG cells were evaluated Results: MAMs isolated from the metastatic lung suppressed NK cell-induced tumor cell apoptosis Conclusion: This study demonstrates that MAMs are a main negative regulator of NK cell function within the metastatic tumor niche, and MAM targeting is an attractive strategy to improve NK cell-based immunotherapy for metastatic breast cancer.
MeSH term(s)	Adoptive Transfer ; Animals ; Antigens, Ly/metabolism ; Breast Neoplasms/immunology ; Breast Neoplasms/pathology ; Breast Neoplasms/therapy ; Female ; Gene Knockout Techniques ; Killer Cells, Natural/immunology ; Killer Cells, Natural/transplantation ; Lung Neoplasms/immunology ; Lung Neoplasms/pathology ; Lung Neoplasms/secondary ; Lung Neoplasms/therapy ; Mice ; Mice, Inbred C57BL ; Natural Cytotoxicity Triggering Receptor 1/metabolism ; Neoplasm Transplantation ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/genetics ; Transforming Growth Factor beta/metabolism ; Tumor-Associated Macrophages/immunology
Chemical Substances	Antigens, Ly ; Csf1r protein, mouse ; Natural Cytotoxicity Triggering Receptor 1 ; Ncr1 protein, mouse ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor ; Transforming Growth Factor beta
Language	English
Publishing date	2021-01-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2719863-7
ISSN	2051-1426 ; 2051-1426
ISSN (online)	2051-1426
ISSN	2051-1426
DOI	10.1136/jitc-2020-001740
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Generation of mouse bone marrow-derived macrophages using tumor coculture assays to mimic the tumor microenvironment.

Güç, Esra / Brownlie, Demi / Rodriguez-Tirado, Carolina / Kitamura, Takanori / Pollard, Jeffrey W

Methods in enzymology

2019 Volume 632, Page(s) 91–111

Abstract: Macrophages are one of the key immune cells within the tumor microenvironment that encourage the growth of tumors at the primary site as well as contributing to all parts of the metastatic cascade. Although it is possible to isolate macrophages directly ... ...

Abstract	Macrophages are one of the key immune cells within the tumor microenvironment that encourage the growth of tumors at the primary site as well as contributing to all parts of the metastatic cascade. Although it is possible to isolate macrophages directly from the tumor, this can be a laborious process and due to their plasticity, it is not possible to maintain their in vivo phenotype in vitro. For this reason, differentiating macrophages from bone marrow is an attractive alternative. Here we present robust methods to study in vitro derived macrophages including (i) the isolation and generation of macrophages from bone marrow, (ii) differentiation/characterization of classically activated, alternatively activated and tumor-conditioned macrophages, as well as (iii) in vitro co-culturing assays for tumor cell-macrophage interaction/transmigration.
MeSH term(s)	Animals ; Bone Marrow Cells/cytology ; Bone Marrow Cells/immunology ; Cell Differentiation ; Cell Line, Tumor ; Cell Separation/methods ; Cells, Cultured ; Coculture Techniques/methods ; Flow Cytometry/methods ; Macrophages/cytology ; Macrophages/immunology ; Mice ; Neoplasms/immunology ; Tumor Microenvironment
Language	English
Publishing date	2019-12-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1557-7988
ISSN (online)	1557-7988
DOI	10.1016/bs.mie.2019.11.014
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Comparison of Lung-Homing Receptor Expression and Activation Profiles on NK Cell and T Cell Subsets in COVID-19 and Influenza.

Brownlie, Demi / Rødahl, Inga / Varnaite, Renata / Asgeirsson, Hilmir / Glans, Hedvig / Falck-Jones, Sara / Vangeti, Sindhu / Buggert, Marcus / Ljunggren, Hans-Gustaf / Michaëlsson, Jakob / Gredmark-Russ, Sara / Smed-Sörensen, Anna / Marquardt, Nicole

Frontiers in immunology

2022 Volume 13, Page(s) 834862

Abstract: Respiratory viral infections with SARS-CoV-2 and influenza viruses commonly induce a strong infiltration of immune cells into the human lung, with potential detrimental effects on the integrity of the lung tissue. Despite comprising the largest fractions ...

Abstract	Respiratory viral infections with SARS-CoV-2 and influenza viruses commonly induce a strong infiltration of immune cells into the human lung, with potential detrimental effects on the integrity of the lung tissue. Despite comprising the largest fractions of circulating lymphocytes in the lung, rather little is known about how peripheral blood natural killer (NK) cell and T cell subsets are equipped for lung-homing in COVID-19 and influenza. Here, we provide a detailed comparative analysis of NK cells and T cells in patients infected with SARS-CoV-2 or influenza virus, focusing on the protein and gene expression of chemokine receptors known to be involved in recruitment to the lung. For this, we used 28-colour flow cytometry as well as re-analysis of a publicly available single-cell RNA-seq dataset from bronchoalveolar lavage (BAL) fluid. Frequencies of NK cells and T cells expressing CXCR3, CXCR6, and CCR5 were altered in peripheral blood of COVID-19 and influenza patients, in line with increased transcript expression of
MeSH term(s)	COVID-19 ; Gene Expression ; Humans ; Influenza, Human/metabolism ; Killer Cells, Natural ; Lung ; SARS-CoV-2 ; T-Lymphocyte Subsets
Language	English
Publishing date	2022-03-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.834862
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Expansions of adaptive-like NK cells with a tissue-resident phenotype in human lung and blood.

Brownlie, Demi / Scharenberg, Marlena / Mold, Jeff E / Hård, Joanna / Kekäläinen, Eliisa / Buggert, Marcus / Nguyen, Son / Wilson, Jennifer N / Al-Ameri, Mamdoh / Ljunggren, Hans-Gustaf / Marquardt, Nicole / Michaëlsson, Jakob

Proceedings of the National Academy of Sciences of the United States of America

2021 Volume 118, Issue 11

Abstract: Human adaptive-like "memory" ... ...

Abstract	Human adaptive-like "memory" CD56
MeSH term(s)	Adaptation, Physiological/immunology ; Flow Cytometry ; Humans ; Immunophenotyping ; Integrin alpha1/immunology ; Killer Cells, Natural/immunology ; Lung/immunology ; Lung Neoplasms/immunology ; Lung Neoplasms/therapy
Chemical Substances	Integrin alpha1
Language	English
Publishing date	2021-04-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2016580118
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1148: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung.

Alisjahbana, Arlisa / Gao, Yu / Sleiers, Natalie / Evren, Elza / Brownlie, Demi / von Kries, Andreas / Jorns, Carl / Marquardt, Nicole / Michaëlsson, Jakob / Willinger, Tim

Frontiers in immunology

2021 Volume 12, Page(s) 752104

Abstract: Innate lymphoid cells (ILCs) contribute to immune defense, yet it is poorly understood how ILCs develop and are strategically positioned in the lung. This applies especially to human ILCs due to the difficulty of studying ... ...

Abstract	Innate lymphoid cells (ILCs) contribute to immune defense, yet it is poorly understood how ILCs develop and are strategically positioned in the lung. This applies especially to human ILCs due to the difficulty of studying them
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Animals ; Animals, Genetically Modified ; CD5 Antigens/immunology ; Cell Differentiation ; Cell Movement ; Cytokines/immunology ; Female ; Hematopoietic Stem Cell Transplantation ; Humans ; Immunity, Innate ; Lung/immunology ; Lymphocytes/immunology ; Male ; Mice ; Middle Aged ; Spleen/immunology
Chemical Substances	CD5 Antigens ; Cytokines
Language	English
Publishing date	2021-11-18
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2021.752104
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Monocytes Differentiate to Immune Suppressive Precursors of Metastasis-Associated Macrophages in Mouse Models of Metastatic Breast Cancer.

Kitamura, Takanori / Doughty-Shenton, Dahlia / Cassetta, Luca / Fragkogianni, Stamatina / Brownlie, Demi / Kato, Yu / Carragher, Neil / Pollard, Jeffrey W

Frontiers in immunology

2018 Volume 8, Page(s) 2004

Abstract: Metastasis-associated macrophages (MAMs) play pivotal roles in breast cancer metastasis by promoting extravasation and survival of metastasizing cancer cells. In a metastatic breast cancer mouse model, we previously reported that circulating classical ... ...

Abstract	Metastasis-associated macrophages (MAMs) play pivotal roles in breast cancer metastasis by promoting extravasation and survival of metastasizing cancer cells. In a metastatic breast cancer mouse model, we previously reported that circulating classical monocytes (C-MOs) preferentially migrated into the tumor-challenged lung where they differentiated into MAMs. However, the fate and characteristics of C-MOs in the metastatic site has not been defined. In this study, we identified that adoptively transferred C-MOs (F4/80
Language	English
Publishing date	2018-01-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606827-8
ISSN	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2017.02004
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mammary Tumor Cells with High Metastatic Potential Are Hypersensitive to Macrophage-Derived HGF.

Kitamura, Takanori / Kato, Yu / Brownlie, Demi / Soong, Daniel Y H / Sugano, Gaël / Kippen, Nicolle / Li, Jiufeng / Doughty-Shenton, Dahlia / Carragher, Neil / Pollard, Jeffrey W

Cancer immunology research

2019 Volume 7, Issue 12, Page(s) 2052–2064

Abstract: Metastasis-associated macrophages (MAM) promote persistent growth of breast cancer cells at the metastatic site and are, thus, an attractive therapeutic target to treat breast cancer metastasis, a leading cause of cancer-related death in women. However, ... ...

Abstract	Metastasis-associated macrophages (MAM) promote persistent growth of breast cancer cells at the metastatic site and are, thus, an attractive therapeutic target to treat breast cancer metastasis, a leading cause of cancer-related death in women. However, the precise mechanisms behind MAM-mediated metastatic tumor outgrowth have not been fully elucidated. Using mouse models of metastatic breast cancer, we showed that MAMs uniquely expressed hepatocyte growth factor (HGF) in metastatic tumors. We also demonstrated that a selected population of cancer cells with high metastatic potential (cancer cells that can establish metastatic tumors in mice with higher number and incidence than parental cells) had higher expression of HGF receptor, MNNG HOS transforming gene (MET), and were more responsive to HGF released from macrophages compared with the parental cells. Blockade of MET signaling in cancer cells suppressed metastatic tumor expansion, in part, through activation of natural killer cells. Results from this study suggest an approach to prevent life-threatening metastatic tumor formation using blockade of MAM-induced MET signal activation in metastatic cancer cells.
MeSH term(s)	Animals ; Cell Line, Tumor ; Female ; Hepatocyte Growth Factor/genetics ; Humans ; Killer Cells, Natural ; Lung/pathology ; Lung Neoplasms/genetics ; Lung Neoplasms/metabolism ; Lung Neoplasms/secondary ; Macrophages/metabolism ; Mammary Neoplasms, Experimental/genetics ; Mammary Neoplasms, Experimental/metabolism ; Mammary Neoplasms, Experimental/pathology ; Mice, Inbred C57BL ; Mice, Inbred NOD ; Mice, SCID ; Mice, Transgenic ; Proto-Oncogene Proteins c-met/genetics ; Proto-Oncogene Proteins c-met/metabolism
Chemical Substances	Hepatocyte Growth Factor (67256-21-7) ; Proto-Oncogene Proteins c-met (EC 2.7.10.1)
Language	English
Publishing date	2019-10-15
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2732489-8
ISSN	2326-6074 ; 2326-6066
ISSN (online)	2326-6074
ISSN	2326-6066
DOI	10.1158/2326-6066.CIR-19-0234
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 7022: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Distinct lung-homing receptor expression and activation profiles on NK cell and T cell subsets in COVID-19 and influenza

bioRxiv

Abstract: Respiratory viral infections with SARS-CoV-2 or influenza viruses commonly induce a strong infiltration of immune cells into the lung, with potential detrimental effects on the integrity of the lung tissue. Despite comprising the largest fractions of ... ...

Abstract	Respiratory viral infections with SARS-CoV-2 or influenza viruses commonly induce a strong infiltration of immune cells into the lung, with potential detrimental effects on the integrity of the lung tissue. Despite comprising the largest fractions of circulating lymphocytes in the lung, little is known about how blood natural killer (NK) cells and T cell subsets are equipped for lung-homing in COVID-19 and influenza. Using 28-colour flow cytometry and re-analysis of published RNA-seq datasets, we provide a detailed comparative analysis of NK cells and T cells in peripheral blood from moderately sick COVID-19 and influenza patients, focusing on the expression of chemokine receptors known to be involved in leukocyte recruitment to the lung. The results reveal a predominant role for CXCR3, CXCR6, and CCR5 in COVID-19 and influenza patients, mirrored by scRNA-seq signatures in peripheral blood and bronchoalveolar lavage from publicly available datasets. NK cells and T cells expressing lung-homing receptors displayed stronger phenotypic signs of activation as compared to cells lacking lung-homing receptors, and activation was overall stronger in influenza as compared to COVID-19. Together, our results indicate migration of functionally competent CXCR3<sup>+</sup>, CXCR6<sup>+</sup>, and/or CCR5<sup>+</sup> NK cells and T cells to the lungs in moderate COVID-19 and influenza patients, identifying potential common targets for future therapeutic interventions in respiratory viral infections.
Keywords	covid19
Language	English
Publishing date	2021-01-13
Publisher	Cold Spring Harbor Laboratory
Document type	Article ; Online
DOI	10.1101/2021.01.13.426553
Database	COVID19

Full text online

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: The Atypical Chemokine Receptor Ackr2 Constrains NK Cell Migratory Activity and Promotes Metastasis.

Hansell, Christopher A H / Fraser, Alasdair R / Hayes, Alan J / Pingen, Marieke / Burt, Claire L / Lee, Kit Ming / Medina-Ruiz, Laura / Brownlie, Demi / Macleod, Megan K L / Burgoyne, Paul / Wilson, Gillian J / Nibbs, Robert J B / Graham, Gerard J

Journal of immunology (Baltimore, Md. : 1950)

2018 Volume 201, Issue 8, Page(s) 2510–2519

Abstract: Chemokines have been shown to be essential players in a range of cancer contexts. In this study, we demonstrate that mice deficient in the atypical chemokine receptor Ackr2 display impaired development of metastasis in vivo in both cell line and ... ...

Abstract	Chemokines have been shown to be essential players in a range of cancer contexts. In this study, we demonstrate that mice deficient in the atypical chemokine receptor Ackr2 display impaired development of metastasis in vivo in both cell line and spontaneous models. Further analysis reveals that this relates to increased expression of the chemokine receptor CCR2, specifically by KLRG1
MeSH term(s)	Animals ; Carcinoma, Lewis Lung ; Cell Movement ; Chemokine CCL2/metabolism ; Cytotoxicity, Immunologic ; Killer Cells, Natural/immunology ; Lectins, C-Type ; Melanoma, Experimental ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neoplasm Metastasis ; Neoplasms, Experimental/immunology ; Receptors, CCR2/metabolism ; Receptors, Chemokine/genetics ; Receptors, Chemokine/metabolism ; Receptors, Immunologic/metabolism
Chemical Substances	Ackr2 protein, mouse ; Ccr2 protein, mouse ; Chemokine CCL2 ; Klrg1 protein, mouse ; Lectins, C-Type ; Receptors, CCR2 ; Receptors, Chemokine ; Receptors, Immunologic
Language	English
Publishing date	2018-08-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.1800131
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.37: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 28: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito