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  1. Article ; Online: First evidence of a mother-to-child transmission of an HIV-1/MO intergroup recombinant form.

    Vautrin, Manon / Tombette, Fabienne / Icard, Vinca / Leoz, Marie / Trabaud, Mary-Anne / Ouziel, Antoine / Panetta, Luc / Lemée, Véronique / Plantier, Jean-Christophe / Moisan, Alice

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

    2024  Volume 30, Issue 5, Page(s) 691–693

    MeSH term(s) Humans ; Infectious Disease Transmission, Vertical ; HIV Infections/transmission ; HIV Infections/virology ; Female ; HIV-1/genetics ; Adult ; Pregnancy ; Recombination, Genetic ; Infant, Newborn ; Infant
    Language English
    Publishing date 2024-02-21
    Publishing country England
    Document type Case Reports ; Letter
    ZDB-ID 1328418-6
    ISSN 1469-0691 ; 1470-9465 ; 1198-743X
    ISSN (online) 1469-0691
    ISSN 1470-9465 ; 1198-743X
    DOI 10.1016/j.cmi.2024.02.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Comparison of eight commercial, high-throughput, automated or ELISA assays detecting SARS-CoV-2 IgG or total antibody.

    Trabaud, Mary-Anne / Icard, Vinca / Milon, Marie-Paule / Bal, Antonin / Lina, Bruno / Escuret, Vanessa

    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    2020  Volume 132, Page(s) 104613

    Abstract: Background: Many commercial assays, of different designs, detecting SARS-CoV-2-specific antibodies exist but with little experience with them.: Objectives: The aim of this study was to compare the performance of assays detecting IgG or total ... ...

    Abstract Background: Many commercial assays, of different designs, detecting SARS-CoV-2-specific antibodies exist but with little experience with them.
    Objectives: The aim of this study was to compare the performance of assays detecting IgG or total antibodies to N or S antigens, validated for routine use in France, with samples from subjects with more or less severe SARS-CoV-2 infection.
    Methods: Eight assays were used: Abbott Architect, DiaSorin Liaison®, bioMérieux Vidas®, Roche Elecsys Cobas®, Siemens Atellica®, BioRad Platelia ELISA, Epitope Diagnostics ELISA, and Wantai ELISA. The tested population included 86 samples from 40 hospitalized subjects and 28 outpatients at different time from symptom onset.
    Results: The positivity rate varied depending on the assay but was greater for all assays in hospitalized than non-hospitalized patients. Despite a good correlation between the assays, discrepancies occurred, without a systematic origin, even for samples taken more than 20 days after symptom onset. These discrepancies were linked to low antibody levels in pauci-symptomatic patients.
    Conclusion: Whichever assay is chosen, a false negative result may need to be ruled out with another test in a risk situation.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Viral/blood ; Automation, Laboratory ; COVID-19/diagnosis ; COVID-19 Testing/methods ; Child ; Enzyme-Linked Immunosorbent Assay/methods ; Female ; High-Throughput Screening Assays ; Humans ; Immunoglobulin G/blood ; Limit of Detection ; Male ; Middle Aged ; Reproducibility of Results ; SARS-CoV-2/immunology ; Serologic Tests ; Young Adult
    Chemical Substances Antibodies, Viral ; Immunoglobulin G
    Keywords covid19
    Language English
    Publishing date 2020-09-07
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article
    ZDB-ID 1446080-4
    ISSN 1873-5967 ; 1386-6532
    ISSN (online) 1873-5967
    ISSN 1386-6532
    DOI 10.1016/j.jcv.2020.104613
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Evaluation of commercial Anti-SARS-CoV-2 neutralizing antibody assays in seropositive subjects.

    Saker, Kahina / Pozzetto, Bruno / Escuret, Vanessa / Pitiot, Virginie / Massardier-Pilonchéry, Amélie / Mokdad, Bouchra / Langlois-Jacques, Carole / Rabilloud, Muriel / Alfaiate, Dulce / Guibert, Nicolas / Fassier, Jean-Baptiste / Bal, Antonin / Trouillet-Assant, Sophie / Trabaud, Mary-Anne

    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    2022  Volume 152, Page(s) 105169

    Abstract: The virus neutralization test (VNT) is the reference for the assessment of the functional ability of neutralizing antibodies (NAb) to block SARS-CoV-2 entry into cells. New competitive immunoassays measuring antibodies preventing interaction between the ... ...

    Abstract The virus neutralization test (VNT) is the reference for the assessment of the functional ability of neutralizing antibodies (NAb) to block SARS-CoV-2 entry into cells. New competitive immunoassays measuring antibodies preventing interaction between the spike protein and its cellular receptor are proposed as surrogate VNT (sVNT). We tested three commercial sVNT (a qualitative immunochromatographic test and two quantitative immunoassays named YHLO and TECO) together with a conventional anti-spike IgG assay (bioMérieux) in comparison with an in-house plaque reduction neutralization test (PRNT
    MeSH term(s) Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19/diagnosis ; Humans ; Neutralization Tests/methods ; SARS-CoV-2
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral
    Language English
    Publishing date 2022-04-27
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1446080-4
    ISSN 1873-5967 ; 1386-6532
    ISSN (online) 1873-5967
    ISSN 1386-6532
    DOI 10.1016/j.jcv.2022.105169
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Comparison of eight commercial, high-throughput, automated or ELISA assays detecting SARS-CoV-2 IgG or total antibody

    Trabaud, Mary-Anne / Icard, Vinca / Milon, Marie-Paule / Bal, Antonin / Lina, Bruno / Escuret, Vanessa

    Journal of Clinical Virology

    2020  Volume 132, Page(s) 104613

    Keywords Virology ; Infectious Diseases ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 1446080-4
    ISSN 1873-5967 ; 1386-6532
    ISSN (online) 1873-5967
    ISSN 1386-6532
    DOI 10.1016/j.jcv.2020.104613
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: A case of acute multidrug resistant HIV-1 infection with acquired immunodeficiency syndrome

    Caroline Charre / Vinca Icard / Mary-Anne Trabaud / Jean-Claude Tardy / Damien Bouhour

    Journal of Virus Eradication, Vol 4, Iss , Pp 23- (2018)

    2018  

    Keywords Microbiology ; QR1-502 ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2018-05-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Comparison of eight commercial, high-throughput, automated or ELISA assays detecting SARS-CoV-2 IgG or total antibody

    Trabaud, Mary-Anne / Icard, Vinca / Milon, Marie-Paule / Bal, Antonin / Lina, Bruno / Escuret, Vanessa

    J Clin Virol

    Abstract: BACKGROUND: Many commercial assays, of different designs, detecting SARS-CoV-2-specific antibodies exist but with little experience with them. OBJECTIVES: The aim of this study was to compare the performance of assays detecting IgG or total antibodies to ...

    Abstract BACKGROUND: Many commercial assays, of different designs, detecting SARS-CoV-2-specific antibodies exist but with little experience with them. OBJECTIVES: The aim of this study was to compare the performance of assays detecting IgG or total antibodies to N or S antigens, validated for routine use in France, with samples from subjects with more or less severe SARS-CoV-2 infection. METHODS: Eight assays were used: Abbott Architect, DiaSorin Liaison®, bioMérieux Vidas®, Roche Elecsys Cobas®, Siemens Atellica®, BioRad Platelia ELISA, Epitope Diagnostics ELISA, and Wantai ELISA. The tested population included 86 samples from 40 hospitalized subjects and 28 outpatients at different time from symptom onset. RESULTS: The positivity rate varied depending on the assay but was greater for all assays in hospitalized than non-hospitalized patients. Despite a good correlation between the assays, discrepancies occurred, without a systematic origin, even for samples taken more than 20 days after symptom onset. These discrepancies were linked to low antibody levels in pauci-symptomatic patients. CONCLUSION: Whichever assay is chosen, a false negative result may need to be ruled out with another test in a risk situation.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #746006
    Database COVID19

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  7. Article ; Online: Are Anti-Receptor-Binding Domain Antibodies Still a Relevant Proxy for Monitoring SARS-CoV-2 Neutralizing Activity in the Omicron Era?

    Saker, Kahina / Pozzetto, Bruno / Bal, Antonin / Valette, Martine / Fassier, Jean Baptiste / Saade, Carla / Trabaud, Mary Anne / Trouillet-Assant, Sophie

    Clinical chemistry

    2022  Volume 68, Issue 7, Page(s) 984–986

    MeSH term(s) Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 ; Humans ; Neutralization Tests ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-05-31
    Publishing country England
    Document type Letter
    ZDB-ID 80102-1
    ISSN 1530-8561 ; 0009-9147
    ISSN (online) 1530-8561
    ISSN 0009-9147
    DOI 10.1093/clinchem/hvac085
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Determinants of protection against SARS-CoV-2 Omicron BA.1 and Delta infections in fully vaccinated outpatients.

    Roy, Alvaro / Saade, Carla / Josset, Laurence / Clément, Bénédicte / Morfin, Florence / Destras, Grégory / Valette, Martine / Icard, Vinca / Billaud, Geneviéve / Oblette, Antoine / Debombourg, Marion / Garrigou, Christine / Brengel-Pesce, Karen / Generenaz, Laurence / Saker, Kahina / Hernu, Romain / Pozzetto, Bruno / Lina, Bruno / Trabaud, Mary-Anne /
    Trouillet-Assant, Sophie / Bal, Antonin

    Journal of medical virology

    2023  Volume 95, Issue 8, Page(s) e28984

    Abstract: We aimed to evaluate the association between the humoral and cellular immune responses and symptomatic SARS-CoV-2 infection with Delta or Omicron BA.1 variants in fully vaccinated outpatients. Anti-receptor binding domain (RBD) IgG levels and interferon- ... ...

    Abstract We aimed to evaluate the association between the humoral and cellular immune responses and symptomatic SARS-CoV-2 infection with Delta or Omicron BA.1 variants in fully vaccinated outpatients. Anti-receptor binding domain (RBD) IgG levels and interferon-gamma (IFN-γ) release were evaluated at PCR-diagnosis of SARS-CoV-2 in 636 samples from negative and positive patients during Delta and Omicron BA.1 periods. Median levels of anti-RBD IgG in positive patients were significantly lower than in negative patients for both variants (p < 0.05). The frequency of Omicron BA.1 infection in patients with anti-RBD IgG concentrations ≥1000 binding antibody units (BAU)/mL was 51.0% and decreased to 34.4% in patients with concentrations ≥3000 BAU/mL. For Delta infection, the frequency of infection was significantly lower when applying the same anti-RBD IgG thresholds (13.3% and 5.3% respectively, p < 0.05). In addition, individuals in the hybrid immunity group had a 4.5 times lower risk of Delta infection compared to the homologous vaccination group (aOR = 0.22, 95% CI: [0.05-0.64]. No significant decrease in the risk of Omicron BA.1 infection was observed in the hybrid group compared to the homologous group, but the risk decreased within the hybrid group as anti-RBD IgG titers increased (aOR = 0.08, 95% CI: [0.01-0.41], p = 0.008). IFN-γ release post-SARS-CoV-2 peptide stimulation was not different between samples from patients infected (either with Delta or Omicron BA.1 variant) or not (p > 0.05). Our results show that high circulating levels of anti-RBD IgG and hybrid immunity were independently associated with a lower risk of symptomatic SARS-CoV-2 infection in outpatients with differences according to the infecting variant (www.clinicaltrials.gov; ID NCT05060939).
    MeSH term(s) Humans ; Outpatients ; SARS-CoV-2 ; COVID-19/prevention & control ; Interferon-gamma ; Hepatitis D ; Immunoglobulin G ; Antibodies, Viral
    Chemical Substances Interferon-gamma (82115-62-6) ; Immunoglobulin G ; Antibodies, Viral
    Language English
    Publishing date 2023-07-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.28984
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Comparison of HIV-1 drug-resistance genotyping by ultra-deep sequencing and sanger sequencing using clinical samples.

    Trabaud, Mary-Anne / Icard, Vinca / Ramière, Christophe / Tardy, Jean-Claude / Scholtes, Caroline / André, Patrice

    Journal of medical virology

    2017  Volume 89, Issue 11, Page(s) 1912–1919

    Abstract: Sanger population sequencing (SPS) is the reference technique to monitor HIV-1-infected patients' therapy. Ultra-deep sequencing (UDS), which allows quantitative detection of drug resistance mutations, may be an alternative method. The study aimed to ... ...

    Abstract Sanger population sequencing (SPS) is the reference technique to monitor HIV-1-infected patients' therapy. Ultra-deep sequencing (UDS), which allows quantitative detection of drug resistance mutations, may be an alternative method. The study aimed to compare reproducibility and predictions of UDS versus SPS in a routine setting. A control containing low-abundance variants was repeatedly tested and clinical plasma samples from 100 patients were prospectively assayed by SPS and UDS using the Roche 454 system. Complete analysis by UDS was available for 88% of samples with various viral loads and subtypes. Comparison of detection thresholds found that SPS sensitivity was variable. Variations found by UDS between 5% to >20% were detected by SPS in 25% to more than 80% of samples. At the 5% cut-off, disagreements were rare and in most cases UDS detected an additional protease secondary mutation, suggesting a possible resistance to a protease inhibitor according to the 2015 ANRS algorithm. Mutations found on reverse transcriptase by only UDS were often explained by previous therapy. UDS with a variant detection threshold at 5% might allow therapy management with minimal differences compared to population sequencing while providing additional information for further determination of pertinent cutoff values for specific resistance mutations.
    MeSH term(s) Anti-HIV Agents/pharmacology ; Anti-HIV Agents/therapeutic use ; Drug Resistance, Viral/genetics ; Female ; Genotype ; Genotyping Techniques ; HIV Infections/drug therapy ; HIV Infections/virology ; HIV-1/drug effects ; HIV-1/genetics ; High-Throughput Nucleotide Sequencing/instrumentation ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Male ; Mutation ; Reproducibility of Results ; Software ; Viral Load/drug effects
    Chemical Substances Anti-HIV Agents
    Language English
    Publishing date 2017-07-06
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.24872
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Six-month antibody response to SARS-CoV-2 in healthcare workers assessed by virus neutralization and commercial assays.

    Bal, Antonin / Trabaud, Mary-Anne / Fassier, Jean-Baptiste / Rabilloud, Muriel / Saker, Kahina / Langlois-Jacques, Carole / Guibert, Nicolas / Paul, Adèle / Alfaiate, Dulce / Massardier-Pilonchery, Amélie / Pitiot, Virginie / Morfin-Sherpa, Florence / Lina, Bruno / Pozzetto, Bruno / Trouillet-Assant, Sophie

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

    2021  Volume 27, Issue 6, Page(s) 933–935

    MeSH term(s) Adult ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; COVID-19/immunology ; Female ; Health Personnel ; Humans ; Male ; Middle Aged ; Neutralization Tests ; SARS-CoV-2/immunology ; Time Factors
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral
    Language English
    Publishing date 2021-01-13
    Publishing country England
    Document type Letter
    ZDB-ID 1328418-6
    ISSN 1469-0691 ; 1470-9465 ; 1198-743X
    ISSN (online) 1469-0691
    ISSN 1470-9465 ; 1198-743X
    DOI 10.1016/j.cmi.2021.01.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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