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  1. Article ; Online: Screening and Characterization of

    Azmani, Asma / Lemriss, Sanaa / Barakate, Mustapha / Souiri, Amal / Dhiba, Driss / Hassani, Lahcen / Hamdali, Hanane

    Biotech (Basel (Switzerland))

    2022  Volume 11, Issue 3

    Abstract: Traditional antimicrobial antibiotics are increasingly suffering from the emergence of multidrug resistance among pathogenic microorganisms. The antibiotic era is threatened by the ruthless rise of resistance in bacterial infections. A significant role ... ...

    Abstract Traditional antimicrobial antibiotics are increasingly suffering from the emergence of multidrug resistance among pathogenic microorganisms. The antibiotic era is threatened by the ruthless rise of resistance in bacterial infections. A significant role in these resistance profiles is attributed to multidrug efflux pumps. Hence, much effort is being directed towards developing new compounds to overcome this problem. During our screening program of efflux pumps inhibitors (EPI) produced by bioactive Moroccan Actinobacteria, 210 isolates were screened for their antibacterial activities against
    Language English
    Publishing date 2022-06-29
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-6284
    ISSN (online) 2673-6284
    DOI 10.3390/biotech11030022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: SARS-CoV-2-Neutralizing Antibody Response and Correlation of Two Serological Assays with Microneutralization.

    Souiri, Amal / Lemriss, Sanaâ / El Maliki, Bouchra / Falahi, Hamadi / El Fahime, Elmostafa / El Kabbaj, Saâd

    Vaccines

    2023  Volume 11, Issue 3

    Abstract: SARS-CoV-2 has caused a huge pandemic affecting millions of people and resulting innumerous deaths. A better understanding of the correlation between binding antibodies and neutralizing antibodies is necessary to address protective immunity post- ... ...

    Abstract SARS-CoV-2 has caused a huge pandemic affecting millions of people and resulting innumerous deaths. A better understanding of the correlation between binding antibodies and neutralizing antibodies is necessary to address protective immunity post-infection or vaccination. Here, we investigate the humoral immune response and the seroprevalence of neutralizing antibodies following vaccination with adenovirus-based vector in 177 serum samples. A Microneutralization (MN) assay was used as a reference method to assess whether neutralizing antibody titers correlated with a positive signal in two commercially available serological tests:a rapid lateral flow immune-chromatographic assay (LFIA) and an enzyme-linked Fluorescence Assay (ELFA). Neutralizing antibodies were detected in most serum samples (84%). COVID-19 convalescent individuals showed high antibody titers and significant neutralizing activity. Spearman correlation coefficients between the serological and neutralization results ranged from 0.8 to 0.9, suggesting a moderate to strong correlation between commercial immunoassays test results (LFIA and ELFA) and virus neutralization.
    Language English
    Publishing date 2023-03-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines11030590
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: SARS-CoV-2-Neutralizing Antibody Response and Correlation of Two Serological Assays with Microneutralization

    Amal Souiri / Sanaâ Lemriss / Bouchra El Maliki / Hamadi Falahi / Elmostafa El Fahime / Saâd El Kabbaj

    Vaccines, Vol 11, Iss 590, p

    2023  Volume 590

    Abstract: SARS-CoV-2 has caused a huge pandemic affecting millions of people and resulting innumerous deaths. A better understanding of the correlation between binding antibodies and neutralizing antibodies is necessary to address protective immunity post- ... ...

    Abstract SARS-CoV-2 has caused a huge pandemic affecting millions of people and resulting innumerous deaths. A better understanding of the correlation between binding antibodies and neutralizing antibodies is necessary to address protective immunity post-infection or vaccination. Here, we investigate the humoral immune response and the seroprevalence of neutralizing antibodies following vaccination with adenovirus-based vector in 177 serum samples. A Microneutralization (MN) assay was used as a reference method to assess whether neutralizing antibody titers correlated with a positive signal in two commercially available serological tests:a rapid lateral flow immune-chromatographic assay (LFIA) and an enzyme-linked Fluorescence Assay (ELFA). Neutralizing antibodies were detected in most serum samples (84%). COVID-19 convalescent individuals showed high antibody titers and significant neutralizing activity. Spearman correlation coefficients between the serological and neutralization results ranged from 0.8 to 0.9, suggesting a moderate to strong correlation between commercial immunoassays test results (LFIA and ELFA) and virus neutralization.
    Keywords SARS-CoV-2 ; serology ; neutralizing antibody ; COVID-19 ; vaccination ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Multiple Potential Plant Growth Promotion Activities of Endemic

    Aallam, Yassine / Maliki, Bouchra El / Dhiba, Driss / Lemriss, Sanaa / Souiri, Amal / Haddioui, Abdelmajid / Tarkka, Mika / Hamdali, Hanane

    Microorganisms

    2021  Volume 9, Issue 7

    Abstract: The characterized ... ...

    Abstract The characterized 10
    Language English
    Publishing date 2021-07-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9071429
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Streptomycetaceae

    Bousselham, Meriam / Lemriss, Sanaa / Dhiba, Driss / Aallam, Yassine / Souiri, Amal / Abbas, Younes / Saïdi, Nezha / Boukcim, Hassan / Hamdali, Hanane

    Microorganisms

    2022  Volume 10, Issue 6

    Abstract: Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four ... ...

    Abstract Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four different rhizospheric Moroccan oat soils and to investigate their potential for P solubilization. The distribution of actinomycetes was significantly more abundant in Settat (9.68%), Tangier (7.38%), and Beni Mellal (6.87%) than in the Merchouch-Rabat (4.90%) region. A total of 235 actinomycete strains were isolated from all sites and tested for their ability to grow on a synthetic minimum medium (SMM) containing insoluble natural rock phosphate (RP) or synthetic tricalcium phosphate (TCP) as the unique P source. One hundred forty-three isolates (60.8%) had the ability to grow in the SMM with RP whereas only twenty-five isolates (17%) had the most active growth using the SMM with TCP. Eight isolates with the most active growth in solid SMM were selected for their P solubilization abilities in liquid SMM cultures. The highest amount of P solubilized was 163.8 µg/mL for RP and 110.27 µg/mL for TCP after 5 days of culture. The biosolubilization process of AM2, the most efficient RP and TCP solubilizing strain, probably implied the highest excretion of siderophore substances. Eight of these strains were shown to belong to the
    Language English
    Publishing date 2022-05-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10061116
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Streptomycetaceae and Promicromonosporaceae

    Meriam Bousselham / Sanaa Lemriss / Driss Dhiba / Yassine Aallam / Amal Souiri / Younes Abbas / Nezha Saïdi / Hassan Boukcim / Hanane Hamdali

    Microorganisms, Vol 10, Iss 1116, p

    Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate

    2022  Volume 1116

    Abstract: Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four ... ...

    Abstract Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four different rhizospheric Moroccan oat soils and to investigate their potential for P solubilization. The distribution of actinomycetes was significantly more abundant in Settat (9.68%), Tangier (7.38%), and Beni Mellal (6.87%) than in the Merchouch-Rabat (4.90%) region. A total of 235 actinomycete strains were isolated from all sites and tested for their ability to grow on a synthetic minimum medium (SMM) containing insoluble natural rock phosphate (RP) or synthetic tricalcium phosphate (TCP) as the unique P source. One hundred forty-three isolates (60.8%) had the ability to grow in the SMM with RP whereas only twenty-five isolates (17%) had the most active growth using the SMM with TCP. Eight isolates with the most active growth in solid SMM were selected for their P solubilization abilities in liquid SMM cultures. The highest amount of P solubilized was 163.8 µg/mL for RP and 110.27 µg/mL for TCP after 5 days of culture. The biosolubilization process of AM2, the most efficient RP and TCP solubilizing strain, probably implied the highest excretion of siderophore substances. Eight of these strains were shown to belong to the Streptomyces genus and one to the Promicromonospora genus. These findings bolster the phosphate biosolubilization abilities of actinomycetes and may participate in increasing agricultural yields in an eco-efficient and environmentally friendly manner.
    Keywords Streptomyces ; Promicromonospora ; biosolubilization ; biofertilizers ; rock phosphate ; Moroccan oat soils ; Biology (General) ; QH301-705.5
    Subject code 500
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Streptomycetaceae and Promicromonosporaceae: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate

    Bousselham, Meriam / Lemriss, Sanaa / Dhiba, Driss / Aallam, Yassine / Souiri, Amal / Abbas, Younes / Saïdi, Nezha / Boukcim, Hassan / Hamdali, Hanane

    Microorganisms. 2022 May 28, v. 10, no. 6

    2022  

    Abstract: Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four ... ...

    Abstract Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four different rhizospheric Moroccan oat soils and to investigate their potential for P solubilization. The distribution of actinomycetes was significantly more abundant in Settat (9.68%), Tangier (7.38%), and Beni Mellal (6.87%) than in the Merchouch-Rabat (4.90%) region. A total of 235 actinomycete strains were isolated from all sites and tested for their ability to grow on a synthetic minimum medium (SMM) containing insoluble natural rock phosphate (RP) or synthetic tricalcium phosphate (TCP) as the unique P source. One hundred forty-three isolates (60.8%) had the ability to grow in the SMM with RP whereas only twenty-five isolates (17%) had the most active growth using the SMM with TCP. Eight isolates with the most active growth in solid SMM were selected for their P solubilization abilities in liquid SMM cultures. The highest amount of P solubilized was 163.8 µg/mL for RP and 110.27 µg/mL for TCP after 5 days of culture. The biosolubilization process of AM2, the most efficient RP and TCP solubilizing strain, probably implied the highest excretion of siderophore substances. Eight of these strains were shown to belong to the Streptomyces genus and one to the Promicromonospora genus. These findings bolster the phosphate biosolubilization abilities of actinomycetes and may participate in increasing agricultural yields in an eco-efficient and environmentally friendly manner.
    Keywords Promicromonospora ; Streptomyces ; biofertilizers ; excretion ; liquids ; oats ; phosphorus ; plant growth ; rock phosphate ; siderophores ; soil ; solubilization ; tricalcium phosphate
    Language English
    Dates of publication 2022-0528
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10061116
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Growth promotion and protection against root rot of sugar beet (Beta vulgaris L.) by two rock phosphate and potassium solubilizing Streptomyces spp. under greenhouse conditions

    Aallam, Yassine / Dhiba, Driss / El Rasafi, Taoufik / Lemriss, Sanaa / Haddioui, Abdelmajid / Tarkka, Mika / Hamdali, Hanane

    Plant and soil. 2022 Mar., v. 472, no. 1-2

    2022  

    Abstract: PURPOSE: Phosphorus (P) and potassium (K) shortages inhibit crop production, and soil borne plant diseases such as root rot by Fusarium spp. can cause extensive damage to crops. Streptomyces bellus (SB) and S. saprophyticus (SS) solubilize P and K and ... ...

    Abstract PURPOSE: Phosphorus (P) and potassium (K) shortages inhibit crop production, and soil borne plant diseases such as root rot by Fusarium spp. can cause extensive damage to crops. Streptomyces bellus (SB) and S. saprophyticus (SS) solubilize P and K and inhibit sugar beet (Beta vulgaris L.) associated Fusarium strains in laboratory conditions. METHODS: To address their performance in vivo, their effects on sugar beet growth and root rot development was tested. RESULTS: The tested strains showed a significant beneficial effect on growth and yield parameters of sugar beet when mixed in the soil with insoluble natural rock phosphate (RP) and/or K mineral orthoclase (OT). Compared to the non-inoculated treatment, the highest shoot and root dry biomass were recorded with RP+OT+SB. Highest P and K levels in leaves were with OT+SB and RP+SB, and the treatment RP+OT+SB increased both soil P and K. Interestingly, these SS and SB strains exhibited high protection effects of 100% and 75%, respectively, when the soil was infested by F. equiseti and F. fujikuroi, causal agents of root rot disease of sugar beet in Beni Mellal region. CONCLUSIONS: These results can be exploited to mitigate the detrimental impacts of nutrient limitation for and disease susceptibility of sugar beet.
    Keywords Beta vulgaris ; Streptomyces bellus ; biomass ; disease susceptibility ; greenhouses ; growth promotion ; phosphorus ; potassium ; rock phosphate ; root rot ; soil ; solubilization ; sugar beet
    Language English
    Dates of publication 2022-03
    Size p. 407-420.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 208908-7
    ISSN 1573-5036 ; 0032-079X
    ISSN (online) 1573-5036
    ISSN 0032-079X
    DOI 10.1007/s11104-021-05252-w
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Eco-friendly degradation of reactive red 195, reactive blue 214, and reactive yellow 145 by Klebsiella pneumoniae MW815592 isolated from textile waste.

    Sennaj, Rajaa / Lemriss, Sanaâ / Souiri, Amal / Kabbaj, Saâd E L / Chafik, Abdelbasset / Essamadi, Abdel Khalid / Benali, Taoufiq / Fassouane, Abdelaziz / Dari, Khadija / Aassila, Hinde

    Journal of microbiological methods

    2022  Volume 204, Page(s) 106659

    Abstract: The water is used in many textile manufacturing steps beyond cleaning. The quantity and the significant chemical load of the effluents generated constitute the primary challenge of the textile industry. In order to discover new sustainable methods to ... ...

    Abstract The water is used in many textile manufacturing steps beyond cleaning. The quantity and the significant chemical load of the effluents generated constitute the primary challenge of the textile industry. In order to discover new sustainable methods to overcome this problem, the aim of this research was to study the potential for degradation of Reactive Blue 214, Reactive Red 195, and Reactive Yellow 145 using a dye degrading bacterium. Sequencing analysis reveals it to be Klebsiella pneumoniae MW815592. This strain completely decolorized artificial effluent (200 mg/L) after 42 h at pH 9 and 46 °C. The decolorization rate increased in the presence of glucose and yeast extract (2 g). In addition, our finding revealed that the decolorization is due to biodegradation rather than adsorption on the bacterial surface.
    MeSH term(s) Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/metabolism ; Coloring Agents/metabolism ; Naphthalenesulfonates ; Azo Compounds/metabolism ; Textiles ; Biodegradation, Environmental
    Chemical Substances reactive red 195 ; Coloring Agents ; Naphthalenesulfonates ; Azo Compounds
    Language English
    Publishing date 2022-12-15
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2022.106659
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Multiple Potential Plant Growth Promotion Activities of Endemic Streptomyces spp. from Moroccan Sugar Beet Fields with Their Inhibitory Activities against Fusarium spp.

    Yassine Aallam / Bouchra El Maliki / Driss Dhiba / Sanaa Lemriss / Amal Souiri / Abdelmajid Haddioui / Mika Tarkka / Hanane Hamdali

    Microorganisms, Vol 9, Iss 1429, p

    2021  Volume 1429

    Abstract: The characterized 10 Streptomyces isolates were previously selected by their abilities to solubilize phosphates. To investigate whether these isolates represent multifaceted plant growth-promoting rhizobacteria (PGPR), their potassium-solubilizing, auxin- ...

    Abstract The characterized 10 Streptomyces isolates were previously selected by their abilities to solubilize phosphates. To investigate whether these isolates represent multifaceted plant growth-promoting rhizobacteria (PGPR), their potassium-solubilizing, auxin-producing and inhibitory activities were determined. The 10 Streptomyces spp. yielded a variable biomass in the presence of insoluble orthoclase as the sole potassium (K) source, indicating that they were able to extract different amounts of K from this source for their own growth. Three strains (AZ, AYD and DE2) released soluble K from insoluble orthoclase in large amounts into the culture broth. The production levels ranged from 125.4 mg/L to 216.6 mg/L after 5 days of culture. Only two strains, Streptomyces enissocaesilis (BYC) and S . tunisiensis (AI), released a larger amount of soluble K from orthoclase and yielded much more biomass. This indicated that the rate of K released from this insoluble orthoclase exceeded its consumption rate for bacterial growth and that some strains solubilized K more efficiently than others. The results also suggest that the K solubilization process of AZ, AYD and DE2 strains, the most efficient K-solubilizing strains, involves a slight acidification of the medium. Furthermore, these 10 Streptomyces spp. were able to secrete indole acetic acid (IAA) in broth medium and ranged from 7.9 ± 0.1 µg/mL to 122.3 ± 0.1 µg/mL. The results of the antibiosis test proved the potential of the 10 tested strains to limit the growth of fungi and bacteria. In dual culture, S . bellus (AYD) had highest inhibitory effect against the three identified fungal causal agents of root rot of sugar beet: Fusarium equiseti and two F . fujikuroi at 55, 43 and 36%, respectively. Streptomyces enissocaesilis (BYC), S . bellus (AYD) and S . saprophyticus (DE2) exhibited higher multifaceted PGPR with their potassium-solubilizing, auxin-producing and inhibitory activities, which could be expected to lead to effectiveness in field trials of sugar beet.
    Keywords Streptomyces spp ; potassium solubilization ; orthoclase ; biocontrol ; root rot ; sugar beet ; Biology (General) ; QH301-705.5
    Subject code 500
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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