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Article ; Online: Average Temperature as a Marker of Lymphoma-Associated HLH.

Clark, Cameron / Goddard, Jack / Tattersall, Rachel / Morley, Nick

2024

Abstract: ... grouped as non-HLH. 12-hour mean temperature at HLH diagnosis was 38.6 °C in the HLH cohort and 37.5 °C ... with HLH status (P = 0.001) and showed high retrospective sensitivity and specificity for HLH above 37.7 °C ...

Abstract	Methods: This retrospective analysis aimed to assess whether a 12-hour mean temperature (measured around either diagnosis of HLH or peak ferritin value) has value as a quick and simple diagnostic test for HLH in people with lymphoproliferative disease (LPD). Hospital records from 2018 to 2022 were retrospectively screened for patients with LPD and peak ferritin during admission to hospital >3000ng/mL. Patients were grouped as either HLH or non-HLH after consensus discussion at a multi-disciplinary meeting with access to full, detailed patient records and H-scores. Results: The total cohort of 23 patients consisted of 12 with HLH and 11 grouped as non-HLH. 12-hour mean temperature at HLH diagnosis was 38.6 °C in the HLH cohort and 37.5 °C measured at the point of peak ferritin measurement in non-HLH groups. It was also positively correlated with HLH status (P = 0.001) and showed high retrospective sensitivity and specificity for HLH above 37.7 °C. Conclusion: These results demonstrate that a 12-hour mean temperature may add value and diagnostic certainty to the first-line investigations for HLH associated with LPD. The moderately high sensitivity and specificity achieved with this dataset supports the need for further research into whether the test retains validity in larger patient groups.
Language	English
Publishing date	2024-03-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	2540992-X
ISSN	2152-2669 ; 2152-2650
ISSN (online)	2152-2669
ISSN	2152-2650
DOI	10.1016/j.clml.2024.02.016
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Article ; Online: Gold nanoparticle-modified sustainable plastic sensor chip for voltammetric monitoring of Hg(II).

Karapa, Alexandra / Kokkinos, Christos / Fielden, Peter R / Baldock, Sara J / Goddard, Nickolas J / Economou, Anastasios

Talanta

2023 Volume 265, Page(s) 124850

Abstract: Mercury is a toxic environmental contaminant that can cause serious health problems. This work describes a new type of eco-friendly three-electrode plastic sensor chip for the determination of trace Hg(II) by means of anodic stripping voltammetry (ASV). ... ...

Abstract	Mercury is a toxic environmental contaminant that can cause serious health problems. This work describes a new type of eco-friendly three-electrode plastic sensor chip for the determination of trace Hg(II) by means of anodic stripping voltammetry (ASV). The sensor chip is entirely fabricated by injection moulding, which is a sustainable manufacturing method, and consists of three conductive carbon-based electrodes embedded in a plastic holder while the reference electrode is coated with Ag using e-beam evaporation. The sample is spiked with Au(III) which deposits on the working electrode in the form of gold nanoparicles during the analysis; the target Hg(II) co-deposits on the gold nanoparticles forming a Au(Hg) amalgam in situ. The accumulated Hg is stripped off the electrode and quantified by an anodic square wave potential scan. The relevant conditions and the potential interferences are investigated. The limit of detection for Hg(II) is 0.4 μg L
Language	English
Publishing date	2023-06-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2023.124850
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Article ; Online: Determination of antimony and barium in UK air quality samples as indicators of non-exhaust traffic emissions.

Goddard, S L / Williams, K R / Robins, C / Brown, R J C

Environmental monitoring and assessment

2019 Volume 191, Issue 11, Page(s) 641

Abstract: The aim of this study was to determine concentrations of antimony and barium in air quality samples and assess the potential suitability of these metals as tracer elements for non-exhaust traffic emissions sources. Air quality filters sampled at ... ...

Abstract	The aim of this study was to determine concentrations of antimony and barium in air quality samples and assess the potential suitability of these metals as tracer elements for non-exhaust traffic emissions sources. Air quality filters sampled at monitoring sites around the UK for the purposes of the UK Heavy Metals Monitoring Network were subjected to microwave digestion in hydrogen peroxide and nitric acid, then analysed by ICP-MS for a suite of metals including antimony and barium. The average antimony concentration found across all the network sites was 1.84 ng m
MeSH term(s)	Air Pollutants/analysis ; Air Pollution/analysis ; Antimony/analysis ; Barium/analysis ; Environmental Monitoring/methods ; United Kingdom ; Vehicle Emissions/analysis
Chemical Substances	Air Pollutants ; Vehicle Emissions ; Barium (24GP945V5T) ; Antimony (9IT35J3UV3)
Language	English
Publishing date	2019-10-05
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	782621-7
ISSN	1573-2959 ; 0167-6369
ISSN (online)	1573-2959
ISSN	0167-6369
DOI	10.1007/s10661-019-7774-8
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Article ; Online: Falling nickel concentrations in ambient air in South Wales - 50 years of progress.

Brown, Richard J C / Goddard, Sharon L / Williams, Katie R / Robins, Chris / Butterfield, David M / Brown, Andrew S

Environmental science. Processes & impacts

2022 Volume 24, Issue 10, Page(s) 1821–1829

Abstract: Measurement of the composition of ambient air has become increasingly widespread over the last 50 years as the detrimental health effects of some air pollutants have become clearer and requirements for these measurements has been embedded in national and ...

Abstract	Measurement of the composition of ambient air has become increasingly widespread over the last 50 years as the detrimental health effects of some air pollutants have become clearer and requirements for these measurements has been embedded in national and international legislation. The aim of this has been not only to assess exposure of the general population to air pollutants but also to assess the effectiveness of abatement strategies to reduce emissions of these pollutants at source. With a rich industrial heritage, the Swansea Valley (South Wales, UK) has long been associated with the refining and production of metal products, especially nickel. Despite a decline in output during the latter part of the twentieth century there is still sufficient activity to prompt a requirement for targeted air monitoring in the area. This is most important for nickel where there is a local history of measured concentrations exceeding legislative target values. This work demonstrates the effectiveness of nickel emissions abatement strategies over the last 50 years by tracking the falling air concentration of nickel over this period. It also demonstrates how the monitoring network in the Swansea Valley has expanded over this time and become significantly more sensitive to nickel emissions. The data presented represents a significant public health achievement - it is likely that the exposure to nickel in air of the population in the Swansea Valley has decreased more than 100-fold over the last 50 years - and reflects the progress in regulation, industrial efficiency, emissions abatement technology and air quality monitoring science achieved during this period.
MeSH term(s)	Humans ; Nickel ; Environmental Monitoring ; Air Pollution/analysis ; Air Pollutants/analysis ; Air
Chemical Substances	Nickel (7OV03QG267) ; Air Pollutants
Language	English
Publishing date	2022-10-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2703814-2
ISSN	2050-7895 ; 2050-7887
ISSN (online)	2050-7895
ISSN	2050-7887
DOI	10.1039/d2em00282e
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Article ; Online: COOLING BY DOUSING WITH COLD WATER DOES NOT ALTER THE PATHOPHYSIOLOGICAL BIOCHEMICAL CHANGES INDUCED BY CAPTURE IN BLESBOK (DAMALISCUS PYGARGUS PHILLIPSI).

Leiberich, Marion / Fitte, Agustina / Burroughs, Richard / Steyl, Johan / Goddard, Amelia / Haw, Anna / Boesch, Jordyn M / Kohn, Tertius A / Meyer, Leith C R

Journal of wildlife diseases

2023 Volume 59, Issue 2, Page(s) 288–298

Abstract: ... Animals in the C+C group were cooled with 10 L of cold water (4 C) for 10 min during immobilization ...

Abstract	Wild animals are commonly captured for conservation, research, and wildlife management purposes. However, capture is associated with a high risk of morbidity or mortality. Capture-induced hyperthermia is a commonly encountered complication believed to contribute significantly to morbidity and mortality. Active cooling of hyperthermic animals by dousing with water is believed to treat capture-induced pathophysiological effects, but remains untested. This study aimed to determine the pathophysiological effects of capture, and whether cooling by dousing with cold water effectively reduces these effects in blesbok (Damaliscus pygargus phillipsi). Thirty-eight blesbok were randomly allocated into three groups: a control group that was not chased (Ct, n=12), chased not cooled (CNC, n=14), and chased plus cooled group (C+C, n=12). The CNC and C+C groups were chased for 15 min prior to chemical immobilization on day 0. Animals in the C+C group were cooled with 10 L of cold water (4 C) for 10 min during immobilization. All animals were immobilized on days 0, 3, 16, and 30. During each immobilization, rectal and muscle temperatures were recorded, and arterial and venous blood samples collected. Blesbok in the CNC and C+C groups presented with capture-induced pathophysiological changes characterized by hyperthermia, hyperlactatemia, increased markers of liver, skeletal, and cardiac muscle damage, hypoxemia, and hypocapnia. Cooling effectively returned body temperatures to normothermic levels, but neither the magnitude nor the duration of the pathophysiological changes differed between the CNC and C+C groups. Therefore, at least in blesbok, capture-induced hyperthermia appears not to be the primary cause of the pathophysiological changes, but is more likely a clinical sign of the hypermetabolism resulting from capture-induced physical and psychological stress. Although cooling is still recommended to prevent the compounding cytotoxic effects of persistent hyperthermia, it is unlikely to prevent stress- and hypoxia-induced damage caused by the capture procedure.
MeSH term(s)	Animals ; Animals, Wild ; Body Temperature ; Water
Chemical Substances	Water (059QF0KO0R)
Language	English
Publishing date	2023-04-26
Publishing country	United States
Document type	Randomized Controlled Trial, Veterinary ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	410709-3
ISSN	1943-3700 ; 0090-3558
ISSN (online)	1943-3700
ISSN	0090-3558
DOI	10.7589/JWD-D-22-00059
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Article ; Online: Eco-friendly voltammetric platform for trace metal determination using a conductive polymer sensor modified with bismuth nanoparticles generated by spark discharge.

Karapa, Alexandra / Kokkinos, Christos / Fielden, Peter R / Baldock, Sara J / Goddard, Nickolas J / Economou, Anastasios / Prodromidis, Mamas I

Mikrochimica acta

2023 Volume 190, Issue 10, Page(s) 376

Abstract: The fabrication of a low-cost eco-friendly sensor platform for the voltammetric determination of trace metals by electrochemical stripping analysis is reported. Plastic conductive electrodes were manufactured via injection moulding from polysterene ... ...

Abstract	The fabrication of a low-cost eco-friendly sensor platform for the voltammetric determination of trace metals by electrochemical stripping analysis is reported. Plastic conductive electrodes were manufactured via injection moulding from polysterene reinforced with carbon fibres. The platform comprises a carbon counter electrode, a working electrode modified with bismuth nanoparticles generated by spark discharge and a reference electrode coated with AgCl. The sensor fabrication and modification procedures are simple, cost-effective and fast while the materials used are environment-friendly. The utility of the voltammetric platform is demonstrated for stripping analysis of Cd(II) and Pb(II); the limits of detection are 0.7 μg L
Language	English
Publishing date	2023-09-02
Publishing country	Austria
Document type	Journal Article
ZDB-ID	89-9
ISSN	1436-5073 ; 0026-3672
ISSN (online)	1436-5073
ISSN	0026-3672
DOI	10.1007/s00604-023-05929-2
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Article ; Online: A common regulatory haplotype doubles lactoferrin concentration in milk.

Lopdell, Thomas J / Trevarton, Alexander J / Moody, Janelle / Prowse-Wilkins, Claire / Knowles, Sarah / Tiplady, Kathryn / Chamberlain, Amanda J / Goddard, Michael E / Spelman, Richard J / Lehnert, Klaus / Snell, Russell G / Davis, Stephen R / Littlejohn, Mathew D

Genetics, selection, evolution : GSE

2024 Volume 56, Issue 1, Page(s) 22

Abstract: Background: Bovine lactoferrin (Lf) is an iron absorbing whey protein with antibacterial, antiviral, and antifungal activity. Lactoferrin is economically valuable and has an extremely variable concentration in milk, partly driven by environmental ... ...

Abstract	Background: Bovine lactoferrin (Lf) is an iron absorbing whey protein with antibacterial, antiviral, and antifungal activity. Lactoferrin is economically valuable and has an extremely variable concentration in milk, partly driven by environmental influences such as milking frequency, involution, or mastitis. A significant genetic influence has also been previously observed to regulate lactoferrin content in milk. Here, we conducted genetic mapping of lactoferrin protein concentration in conjunction with RNA-seq, ChIP-seq, and ATAC-seq data to pinpoint candidate causative variants that regulate lactoferrin concentrations in milk. Results: We identified a highly-significant lactoferrin protein quantitative trait locus (pQTL), as well as a cis lactotransferrin (LTF) expression QTL (cis-eQTL) mapping to the LTF locus. Using ChIP-seq and ATAC-seq datasets representing lactating mammary tissue samples, we also report a number of regions where the openness of chromatin is under genetic influence. Several of these also show highly significant QTL with genetic signatures similar to those highlighted through pQTL and eQTL analysis. By performing correlation analysis between these QTL, we revealed an ATAC-seq peak in the putative promotor region of LTF, that highlights a set of 115 high-frequency variants that are potentially responsible for these effects. One of the 115 variants (rs110000337), which maps within the ATAC-seq peak, was predicted to alter binding sites of transcription factors known to be involved in lactation-related pathways. Conclusions: Here, we report a regulatory haplotype of 115 variants with conspicuously large impacts on milk lactoferrin concentration. These findings could enable the selection of animals for high-producing specialist herds.
MeSH term(s)	Animals ; Female ; Haplotypes ; Lactation/genetics ; Lactoferrin/genetics ; Lactoferrin/analysis ; Lactoferrin/metabolism ; Milk/chemistry ; Milk/metabolism ; Cattle
Chemical Substances	Lactoferrin (EC 3.4.21.-)
Language	English
Publishing date	2024-03-28
Publishing country	France
Document type	Journal Article
ZDB-ID	1005838-2
ISSN	1297-9686 ; 0754-0264 ; 0999-193X
ISSN (online)	1297-9686
ISSN	0754-0264 ; 0999-193X
DOI	10.1186/s12711-024-00890-x
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Article ; Online: Most people share genetic test results with relatives even if the findings are normal: Family communication in a diverse population.

Hunter, Jessica Ezzell / Riddle, Leslie / Joseph, Galen / Amendola, Laura M / Gilmore, Marian J / Zepp, Jamilyn M / Shuster, Elizabeth / Bulkley, Joanna E / Muessig, Kristin R / Anderson, Katherine P / Goddard, Katrina A B / Wilfond, Benjamin S / Leo, Michael C

Genetics in medicine : official journal of the American College of Medical Genetics

2023 Volume 25, Issue 11, Page(s) 100923

Abstract: Purpose: With increasing utilization of genetic testing, sharing genetic information can become part of general family health communication while providing biological relatives with important information about their own genetic risk. Importantly, little ...

Abstract	Purpose: With increasing utilization of genetic testing, sharing genetic information can become part of general family health communication while providing biological relatives with important information about their own genetic risk. Importantly, little is known about motivations for and barriers to family communication of genetic information in historically underserved populations. Methods: Using mixed methods, we explored patient experiences with family communication in a study population of English- and Spanish-speaking adults aged 18 to 49 years, enriched for participants from historically underserved backgrounds. Risk screening for hereditary cancer guided genetic testing for cancer risk genes and other medically actionable findings. Results: Most participants overall (91%), including most with normal findings (89%), shared or planned to share their results with relatives. Common motivations for sharing results were to give relatives information about their genetic risk and because the participant thought the results were interesting. Reasons for not sharing were limited contact with relatives, perceptions of limited clinical utility for relatives, and concern that discussion of genetic information was stigmatized or taboo. Conclusion: Results demonstrate high rates of sharing genetic information, indicate motivations for sharing go beyond facilitating genetic testing for relatives, and suggest general willingness to share genetic information as part of family health communication.
MeSH term(s)	Adult ; Humans ; Genetic Testing/methods ; Communication ; Neoplasms/genetics ; Family ; Surveys and Questionnaires ; Genetic Predisposition to Disease
Language	English
Publishing date	2023-07-05
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	1455352-1
ISSN	1530-0366 ; 1098-3600
ISSN (online)	1530-0366
ISSN	1098-3600
DOI	10.1016/j.gim.2023.100923
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Article ; Online: Livestock microbial landscape patterns: Retail poultry microbiomes significantly vary by region and season.

Schofield, B J / Andreani, N A / Günther, C S / Law, G R / McMahon, G / Swainson, M / Goddard, M R

Food microbiology

2021 Volume 101, Page(s) 103878

Abstract: Microbes play key roles in animal welfare and food safety but there is little understanding of whether microbiomes associated with livestock vary in space and time. Here we analysed the bacteria associated with the carcasses of the same breed of 28 ... ...

Abstract	Microbes play key roles in animal welfare and food safety but there is little understanding of whether microbiomes associated with livestock vary in space and time. Here we analysed the bacteria associated with the carcasses of the same breed of 28 poultry broiler flocks at different stages of processing across two climatically similar UK regions over two seasons with 16S metabarcode DNA sequencing. Numbers of taxa types did not differ by region, but did by season (P = 1.2 × 10
MeSH term(s)	Animals ; Chickens/microbiology ; Livestock/microbiology ; Microbiota ; Poultry/microbiology ; RNA, Ribosomal, 16S ; Seasons ; United Kingdom
Chemical Substances	RNA, Ribosomal, 16S
Language	English
Publishing date	2021-08-05
Publishing country	England
Document type	Journal Article
ZDB-ID	50892-5
ISSN	1095-9998 ; 0740-0020
ISSN (online)	1095-9998
ISSN	0740-0020
DOI	10.1016/j.fm.2021.103878
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Article: Determination of antimony and barium in UK air quality samples as indicators of non-exhaust traffic emissions

Goddard, S. L / Williams, K. R / Robins, C / Brown, R. J. C

Environmental monitoring and assessment. 2019 Nov., v. 191, no. 11

2019

Abstract	The aim of this study was to determine concentrations of antimony and barium in air quality samples and assess the potential suitability of these metals as tracer elements for non-exhaust traffic emissions sources. Air quality filters sampled at monitoring sites around the UK for the purposes of the UK Heavy Metals Monitoring Network were subjected to microwave digestion in hydrogen peroxide and nitric acid, then analysed by ICP-MS for a suite of metals including antimony and barium. The average antimony concentration found across all the network sites was 1.84 ng m⁻³; the average barium concentration was 6.33 ng m⁻³. The range of antimony concentrations observed was 0.13–8.02 ng m⁻³; barium concentrations ranged from levels below the detection limit of 0.18 to 39.9 ng m⁻³. There are no legislative limits for antimony and barium in ambient air, but the maximum concentrations found are well below the Workplace Exposure Limits specified by the UK Health and Safety Executive. The highest concentrations were found at roadside sites situated to monitor traffic environments, supporting the suitability of antimony and barium to be considered tracer elements for traffic emissions sources. Strong correlations were observed between antimony, barium and copper, indicating they share a common traffic-related source. Based on the strong correlation with copper at urban and traffic locations, indicative annual UK atmospheric emission estimates for antimony and barium in brake and tyre wear were calculated as 6 and 19 tonnes respectively.
Keywords	air ; air quality ; antimony ; barium ; copper ; detection limit ; emissions ; filters ; heavy metals ; hydrogen peroxide ; microwave treatment ; monitoring ; nitric acid ; roadsides ; tires ; traffic ; working conditions ; United Kingdom
Language	English
Dates of publication	2019-11
Size	p. 641.
Publishing place	Springer International Publishing
Document type	Article
ZDB-ID	782621-7
ISSN	1573-2959 ; 0167-6369
ISSN (online)	1573-2959
ISSN	0167-6369
DOI	10.1007/s10661-019-7774-8
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