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Article ; Online: Comprehensive overview of COVID-19-related respiratory failure: focus on cellular interactions.

Zamani Rarani, Fahimeh / Zamani Rarani, Mohammad / Hamblin, Michael R / Rashidi, Bahman / Hashemian, Seyed Mohammad Reza / Mirzaei, Hamed

Cellular & molecular biology letters

2022 Volume 27, Issue 1, Page(s) 63

Abstract: The pandemic outbreak of coronavirus disease 2019 (COVID-19) has created health challenges in all parts of the world. Understanding the entry mechanism of this virus into host cells is essential for effective treatment of COVID-19 disease. This virus can ...

Abstract	The pandemic outbreak of coronavirus disease 2019 (COVID-19) has created health challenges in all parts of the world. Understanding the entry mechanism of this virus into host cells is essential for effective treatment of COVID-19 disease. This virus can bind to various cell surface molecules or receptors, such as angiotensin-converting enzyme 2 (ACE2), to gain cell entry. Respiratory failure and pulmonary edema are the most important causes of mortality from COVID-19 infections. Cytokines, especially proinflammatory cytokines, are the main mediators of these complications. For normal respiratory function, a healthy air-blood barrier and sufficient blood flow to the lungs are required. In this review, we first discuss airway epithelial cells, airway stem cells, and the expression of COVID-19 receptors in the airway epithelium. Then, we discuss the suggested molecular mechanisms of endothelial dysfunction and blood vessel damage in COVID-19. Coagulopathy can be caused by platelet activation leading to clots, which restrict blood flow to the lungs and lead to respiratory failure. Finally, we present an overview of the effects of immune and non-immune cells and cytokines in COVID-19-related respiratory failure.
MeSH term(s)	COVID-19 ; Cytokines ; Humans ; Peptidyl-Dipeptidase A ; Respiratory Insufficiency ; SARS-CoV-2
Chemical Substances	Cytokines ; Peptidyl-Dipeptidase A (EC 3.4.15.1)
Language	English
Publishing date	2022-07-30
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2108724-6
ISSN	1689-1392 ; 1689-1392
ISSN (online)	1689-1392
ISSN	1689-1392
DOI	10.1186/s11658-022-00363-3
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Article ; Online: Correlation between sperm motility and sperm chromatin/DNA damage before and after cryopreservation and the effect of folic acid and nicotinic acid on post-thaw sperm quality in normozoospermic men.

Rarani, Fahimeh Zamani / Golshan-Iranpour, Farhad / Dashti, Gholam Reza

Cell and tissue banking

2019 Volume 20, Issue 3, Page(s) 367–378

Abstract: Cryopreservation exposes sperm to physical and chemical stresses causing cell damages and impairs sperm functions. The aim of this study was to evaluate the association between motility and sperm chromatin/DNA damage before and after cryopreservation and ...

Abstract	Cryopreservation exposes sperm to physical and chemical stresses causing cell damages and impairs sperm functions. The aim of this study was to evaluate the association between motility and sperm chromatin/DNA damage before and after cryopreservation and investigate the effects of folic acid and nicotinic acid on post-thaw sperm quality. Thirty semen samples were obtained from 30 normozoospermic men, aged between 25 and 45 years old. Each sample were divided into five aliquots to form the following groups: fresh, cryopreserved with sperm-freeze only (control), with nicotinic acid (10 mM), with folic acid (50 nM), and with a combination of folic acid (50 nM) + nicotinic acid (10 mM). Sperm viability and motility in each group were assessed by eosin-nigrosine staining and computer-aided sperm analysis respectively. Sperm chromatin quality was studied by aniline blue, toluidine blue, acridine orange staining methods and sperm chromatin dispersion test. Cryopreservation led to a significant reduction in sperm quality in comparison to fresh sample groups (p < 0.05). Sperm chromatin damage was negatively correlated with the percentage of progressively motile cells. Supplementation of the cryopreservation medium with folic acid or nicotinic acid induced a significant improvement in sperm parameters and chromatin quality, compared to control groups (p < 0.05). Meanwhile, the combination of folic acid + nicotinic acid showed a significant protective effect in post thaw sperm. In conclusion, cryopreservation generated oxidative stress, inducingsperm cryodamage, reducing progressive motility and sperm quality, as an indicator of significant chromatin/DNA damage. Folic acid and nicotinic acid exhibited a potential cryoprotective effect by enhancing sperm quality.
MeSH term(s)	Acrosome/drug effects ; Adult ; Chromatin/chemistry ; Cryopreservation ; Cryoprotective Agents/pharmacology ; DNA Damage ; Folic Acid/pharmacology ; Humans ; Male ; Middle Aged ; Niacin/pharmacology ; Semen Analysis/methods ; Semen Preservation/methods ; Sperm Motility ; Spermatozoa/drug effects
Chemical Substances	Chromatin ; Cryoprotective Agents ; Niacin (2679MF687A) ; Folic Acid (935E97BOY8)
Language	English
Publishing date	2019-05-28
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2170897-6
ISSN	1573-6814 ; 1389-9333
ISSN (online)	1573-6814
ISSN	1389-9333
DOI	10.1007/s10561-019-09775-6
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Zs.A 5972: Show issues

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Article ; Online: The role of microRNAs in the pathophysiology of human central nervous system: A focus on neurodegenerative diseases.

Rezaee, Delsuz / Saadatpour, Fatemeh / Akbari, Nayyereh / Zoghi, Anahita / Najafi, Sajad / Beyranvand, Parisa / Zamani-Rarani, Fahimeh / Rashidi, Mohammad Amin / Bagheri-Mohammadi, Saeid / Bakhtiari, Mohammad

Ageing research reviews

2023 Volume 92, Page(s) 102090

Abstract: microRNAs (miRNAs) are suggested to play substantial roles in regulating the development and various physiologic functions of the central nervous system (CNS). These include neurogenesis, cell fate and differentiation, morphogenesis, formation of ... ...

Abstract	microRNAs (miRNAs) are suggested to play substantial roles in regulating the development and various physiologic functions of the central nervous system (CNS). These include neurogenesis, cell fate and differentiation, morphogenesis, formation of dendrites, and targeting non-neural mRNAs. Notably, deregulation of an increasing number of miRNAs is associated with several neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, multiple sclerosis, amyotrophic lateral sclerosis and CNS tumors. They are particularly known to affect the amyloid β (Aβ) cleavage and accumulation, tau protein homeostasis, and expression of alpha-synuclein (α-syn), Parkin, PINK1, and brain-derived neurotrophic factor (BDNF) that play pivotal roles in the pathogenesis of neurodegenerative diseases. These include miR-16, miR-17-5p, miR-20a, miR-106a, miR-106b, miR-15a, miR-15b, miR-103, miR-107, miR-298, miR-328, miR-195, miR-485, and miR-29. In CNS tumors, several miRNAs, including miR-31, miR-16, and miR-21 have been identified to modulate tumorigenesis through impacting tumor invasion and apoptosis. In this review article, we have a look at the recent advances on our knowledge about the role of miRNAs in human brain development and functions, neurodegenerative diseases, and their clinical potentials.
MeSH term(s)	Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Neurodegenerative Diseases/metabolism ; Amyloid beta-Peptides ; Alzheimer Disease/genetics ; Alzheimer Disease/metabolism ; Central Nervous System/metabolism ; Neoplasms
Chemical Substances	MicroRNAs ; Amyloid beta-Peptides ; MIRN298 microRNA, human ; MIRN485 microRNA, human
Language	English
Publishing date	2023-10-11
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2075672-0
ISSN	1872-9649 ; 1568-1637
ISSN (online)	1872-9649
ISSN	1568-1637
DOI	10.1016/j.arr.2023.102090
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Zs.A 5579: Show issues

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Article: Cytokines and microRNAs in SARS-CoV-2: What do we know?

Rarani, Fahimeh Zamani / Rashidi, Bahman / Jafari Najaf Abadi, Mohammad Hassan / Hamblin, Michael R / Reza Hashemian, Seyed Mohammad / Mirzaei, Hamed

Molecular therapy. Nucleic acids

2022 Volume 29, Page(s) 219–242

Abstract: The coronavirus disease 2019 (COVID-19) pandemic constitutes a global health emergency. Currently, there are no completely effective therapeutic medications for the management of this outbreak. The cytokine storm is a hyperinflammatory medical condition ... ...

Abstract	The coronavirus disease 2019 (COVID-19) pandemic constitutes a global health emergency. Currently, there are no completely effective therapeutic medications for the management of this outbreak. The cytokine storm is a hyperinflammatory medical condition due to excessive and uncontrolled release of pro-inflammatory cytokines in patients suffering from severe COVID-19, leading to the development of acute respiratory distress syndrome (ARDS) and multiple organ dysfunction syndrome (MODS) and even mortality. Understanding the pathophysiology of COVID-19 can be helpful for the treatment of patients. Evidence suggests that the levels of tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1 and IL-6 are dramatically different between mild and severe patients, so they may be important contributors to the cytokine storm. Several serum markers can be predictors for the cytokine storm. This review discusses the cytokines involved in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, focusing on interferons (IFNs) and ILs, and whether they can be used in COVID-19 treatment. Moreover, we highlight several microRNAs that are involved in these cytokines and their role in the cytokine storm caused by COVID-19.
Language	English
Publishing date	2022-06-25
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2662631-7
ISSN	2162-2531
ISSN	2162-2531
DOI	10.1016/j.omtn.2022.06.017
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Article ; Online: Endometrial pinopode biomarkers: Molecules and microRNAs.

Rarani, Fahimeh Zamani / Borhani, Fatemeh / Rashidi, Bahman

Journal of cellular physiology

2018 Volume 233, Issue 12, Page(s) 9145–9158

Abstract: Ultrastructural changes on the apical surface of the luminal epithelium of the uterus are known as pinopodes. Their morphology in species and in special species is associated with different results about size, duration, and percentage of surface area ... ...

Abstract	Ultrastructural changes on the apical surface of the luminal epithelium of the uterus are known as pinopodes. Their morphology in species and in special species is associated with different results about size, duration, and percentage of surface area covered by pinopodes. The content of pinopodes is different in rodents and humans. In mice and rats pinopodes have many vacuoles and no organelle that extends to the actin stalk above the microvilli. Human pinopodes do not have a large vacuole and contain the golgi complex, a rough endoplasmic reticulum, secretory vesicles, and mitochondria that extend from the entire cell surface. It has been suggested that pinopodes are good markers of endometrial receptivity and implantation window. There are several molecular markers related to the presence of pinopodes, including integrins, leukemia inhibiting factor (LIF), l-selectin, HOXA10, glutaredoxin, glycodelinA, heparin-binding epidermal growth factor, mucins, and microRNAs (miRNAs). Multiple lines of evidence have indicated that miRNAs could affect the expression of LIF and pinopodes in the endometrium and these molecules play key roles in implantation window processes. Here, we have summarized the morphology and function of pinopodes. Moreover, we have highlighted several molecules in relation to pinopodes that could be used as biomarkers.
MeSH term(s)	Animals ; Biomarkers/metabolism ; Endoplasmic Reticulum, Rough/genetics ; Endoplasmic Reticulum, Rough/ultrastructure ; Epithelium/metabolism ; Epithelium/ultrastructure ; Female ; Golgi Apparatus/genetics ; Golgi Apparatus/ultrastructure ; Homeodomain Proteins/genetics ; Humans ; L-Selectin/genetics ; Leukemia Inhibitory Factor/genetics ; Mice ; MicroRNAs/genetics ; Rats ; Uterus/metabolism ; Uterus/ultrastructure
Chemical Substances	Biomarkers ; Homeodomain Proteins ; Leukemia Inhibitory Factor ; MicroRNAs ; L-Selectin (126880-86-2) ; HOXA10 protein, human (140441-81-2)
Language	English
Publishing date	2018-07-03
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	3116-1
ISSN	1097-4652 ; 0021-9541
ISSN (online)	1097-4652
ISSN	0021-9541
DOI	10.1002/jcp.26852
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Article: microRNA-184 in the landscape of human malignancies: a review to roles and clinical significance.

Fattahi, Mehdi / Rezaee, Delsuz / Fakhari, Fatemeh / Najafi, Sajad / Aghaei-Zarch, Seyed Mohsen / Beyranvand, Parisa / Rashidi, Mohammad Amin / Bagheri-Mohammadi, Saeid / Zamani-Rarani, Fahimeh / Bakhtiari, Mohammad / Bakhtiari, Abbas / Falahi, Shahab / Kenarkoohi, Azra / Majidpoor, Jamal / Nguyen, P U

Cell death discovery

2023 Volume 9, Issue 1, Page(s) 423

Abstract: MicroRNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) with a short length of 19-22 nucleotides. miRNAs are posttranscriptional regulators of gene expression involved in various biological processes like cell growth, apoptosis, and angiogenesis. miR- ... ...

Abstract	MicroRNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) with a short length of 19-22 nucleotides. miRNAs are posttranscriptional regulators of gene expression involved in various biological processes like cell growth, apoptosis, and angiogenesis. miR-184 is a well-studied miRNA, for which most studies report its downregulation in cancer cells and tissues and experiments support its role as a tumor suppressor inhibiting malignant biological behaviors of cancer cells in vitro and in vivo. To exert its functions, miR-184 affects some signaling pathways involved in tumorigenesis like Wnt and β-catenin, and AKT/mTORC1 pathway, oncogenic factors (e.g., c-Myc) or apoptotic proteins, such as Bcl-2. Interestingly, clinical investigations have shown miR-184 with good performance as a prognostic/diagnostic biomarker for various cancers. Additionally, exogenous miR-184 in cell and xenograft animal studies suggest it as a therapeutic anticancer target. In this review, we outline the studies that evaluated the roles of miR-184 in tumorigenesis as well as its clinical significance.
Language	English
Publishing date	2023-11-24
Publishing country	United States
Document type	Journal Article ; Review
ISSN	2058-7716
ISSN	2058-7716
DOI	10.1038/s41420-023-01718-1
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Article ; Online: Effect of chromatin condensation on frozen-thawed sperm DNA integrity in normozoospermic men

farhad Golshan-Iranpour / Fahimeh Zamani Rarani / Gholam Reza Dashti

مجله علمی دانشگاه علوم پزشکی کردستان, Vol 24, Iss 3, Pp 34-

2019 Volume 42

Abstract: Background and Aim: Sperm cryopreservation is a common technique used for management of male infertility. But this method has detrimental effects on sperm DNA and chromatin quality. Evaluation of different markers associated with the health of genetic ... ...

Abstract	Background and Aim: Sperm cryopreservation is a common technique used for management of male infertility. But this method has detrimental effects on sperm DNA and chromatin quality. Evaluation of different markers associated with the health of genetic material of sperm is beneficial for determination of the fertility of sperm. The aim of this study was to assess the effect of sperm chromatin condensation on frozen-thawed sperm DNA integrity in normozoospermic men. Material and Methods: In this experimental study, 30 semen samples from normozospermia men were cryopreserved for two weeks with a common technique used in most infertility centers, at -196 ° C and then thawed. Samples before and after freezing were evaluated for abnormal chromatin condensation, DNA denaturation and DNA fragmentation by toluidine blue (TB) staining, acridine orange (AO) staining and sperm chromatin dispersion (SCD) test respectively. Results: Before freezing, we found a significant correlation only between abnormal chromatin condensation (evaluated by TB) and DNA denaturation (assessed by AO) (p < 0.05). While after cryopreservation correlation was found between abnormal chromatin condensation and DNA denaturation and fragmentation (p < 0.05). Conclusion: Abnormal chromatin condensation can make DNA susceptible to denaturation and fragmentation.
Keywords	sperm ; dna ; chromatin ; normozoospermic ; Medicine ; R ; Medicine (General) ; R5-920
Subject code	571
Language	Persian
Publishing date	2019-07-01T00:00:00Z
Publisher	Kurdistan University of Medical Sciences
Document type	Article ; Online
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Article ; Online: Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma

Fahimeh Zamani-Rarani / Zeinolabedin Shrifian-Dastjerdi / Ali Valiani / Mohammad Zamani-Rarani / Elias Kargar-Abargouei / Ebrahim Eftekhar / Majid Pourentezari / Javad Mohajer-Ansari

مجله دانشکده پزشکی اصفهان, Vol 38, Iss 601, Pp 875-

2021 Volume 881

Abstract: Background: Improper prognosis in brain cancers requires new treatments. Using family of purinergic receptors with confirmed apoptotic effect can be beneficial. As the role of type A1 receptor in multiform glioblastoma in relation to the P53 gene and ... ...

Abstract	Background: Improper prognosis in brain cancers requires new treatments. Using family of purinergic receptors with confirmed apoptotic effect can be beneficial. As the role of type A1 receptor in multiform glioblastoma in relation to the P53 gene and apoptotic pathways is nor reported, we studied the role of agonist (N6-cyclopentyladenosine or CPA) and antagonist (8-cyclopentyl-1,3-dipropylxanthine or DPCPX) of this receptor on cell apoptosis and also expression of P53 genes and caspases 7, 8, and 9. Methods: In this study, MTT assay was used to investigate the rate of cellular proliferation, and flowcytometry method with annexin and Pi was also used to investigate early and late cell apoptosis. To evaluate the internal and external apoptotic pathways expression of P53 genes and caspases 7, 8, and 9, real-time reverse transcription polymerase chain reaction (real-time RT PCR) was used. Findings: The treatment of U87Mg cells with DPCPX increased the expression of P53 gene. Expression of caspase 7 as an executive caspase and caspase 9 as a caspase of the mitochondrial pathway of apoptosis increased, but no expression change was observed in the caspase 8 gene. Conclusion: The results of MTT and flowcytometry showed that DPCPX, in addition to suppressing cell proliferation, stimulated apoptosis in U87Mg cells. Inhibition of adenosine A1 receptors by stimulating the expression of genes involved in apoptotic pathways, especially mitochondrial pathway genes, suppressed cell proliferation and induced apoptosis in U87Mg cells.
Keywords	receptor ; adenosine a1 ; apoptosis ; p53 genes ; Medicine ; R ; Medicine (General) ; R5-920
Subject code	570
Language	Persian
Publishing date	2021-02-01T00:00:00Z
Publisher	Vesnu Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Evaluation of Lomustine-Loaded Iron Nanoparticles on Caspase-6 Gene Expression and Cell Viability in U87Mg Cell Line

Zeinolabedin Sharifian Dastjerdi / Elyas Kargar Abargouei / Salman Jafari / Ebrahim Eftekhar / Majid Pourentezari / Fahimeh Zamani Rarani / Reza Afzalipour / Javad Mohajer Ansari / Mohammad Zamani Rarani

Disease and Diagnosis, Vol 9, Iss 3, Pp 124-

2020 Volume 129

Abstract: Background: Every year, many people around the world die from cancers. Among all types of cancers, brain cancer has been recognized as one of the most deadly cancers due to the late detection and limitations of current therapies, and thus it remains an ... ...

Abstract	Background: Every year, many people around the world die from cancers. Among all types of cancers, brain cancer has been recognized as one of the most deadly cancers due to the late detection and limitations of current therapies, and thus it remains an unresolved problem. Glioblastoma occurs in different parts of the central nervous system and is one of the most important causes of cancer death in people. In addition, there are many problems for the treatment of cancer cells. One of the limiting factors is the resistance of cancer cells to chemotherapy drugs. In this regard, the use of nanoparticles (NPs) is an effective method for overcoming this problem. Materials and Methods: In this study, iron oxide-NPs were synthesized and loaded on the folic and lomustine. Further, the size and morphology of NPs were assessed by transmission electron microscopy, X-ray photoelectron spectroscopy, and dynamic light scattering. Then, the U87-MG cell line was cultured in the Dulbecco’s Modified Eagle Medium and treated with nano, nano-folic, nano-lomustine (LUM), LUM, and complex, followed by evaluating 50% inhibitory concentration, tetrazolium assay, and caspase-6 activity. Results: Our results showed that cell viability decreased in LUM container groups by increasing the incubation time. Based on the caspase-6 activity analysis, the mortality rate increased in LUM container groups after 3 days. These findings indicated that LUM, complex, and nano-LUM increase cell death in U87MG. Conclusion: Finally, the results suggested that LUM in NPs could be applied as a safer form of drug delivery for targeting cancer.
Keywords	targeted therapy ; nanoparticle ; apoptosis ; caspase ; u87mg ; Medicine ; R
Subject code	610
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	Hormozgan University of Medical Sciences
Document type	Article ; Online
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Article ; Online: Adenosine A1 Receptor Antagonist Up-regulates Casp3 and Stimulates Apoptosis Rate in Breast Cancer Cell Line T47D

Mohammad Zamani Rarani / Fahimeh Zamani Rarani / Ali Valiani / Zeinolabedin Shrifian Dastjerdi / Elias Kargar Abargouei / Ebrahim Eftekhar / Asghar Taheri Kafran / Majid Pourentezari / Malihe Saghebray / Farnoosh Razavi / Sanaz Hadizadeh / Mehdi Nikbakht Dastjerdi

Disease and Diagnosis, Vol 9, Iss 1, Pp 14-

2020 Volume 20

Abstract: Background: Adenosine receptor family, especially A1 type is-overexpressed in breast-derived tumor cells and the P53 gene is mutant in some of these cells while the casps gene is of wild type as well. The aim of this study was to evaluate the effect of ... ...

Abstract	Background: Adenosine receptor family, especially A1 type is-overexpressed in breast-derived tumor cells and the P53 gene is mutant in some of these cells while the casps gene is of wild type as well. The aim of this study was to evaluate the effect of the A1 receptor function on cell programmed death or proliferation, as well as the relationship between this receptor stimulation/inhibition and caspase 3 (casp3) expression in T47D cell line that has a mutant and non-functional P53 gene. Materials and Methods: The expression of casps3 was measured by real-time polymerase chain reaction and then flow cytometery and MTT assay were used to assess the apoptotic and proliferation cell rate after the treatment of T47D cells with specific agonist N6-cyclopentyladenosine (CPA) and antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) of this receptor 24, 48, and 72 hours after treatment. Result: Our results indicated that DPCPX significantly induces apoptosis in T47D cells and the rate of survival cell after the reduction of this treatment, especially 72 hours after treatment. Finally, the expression of casp3 was up-regulated by DPCPX treatment, especially in 72 hours while CPA treatment had opposite results (P>0.05). Conclusion: In general, DPCPX could up-regulate casp3 gene expression and subsequently increase the apoptosis rate in T47D cells with casp3 expression without the P53 gene interference. Therefore, adenosine A1 receptor antagonists may be introduced as anti-cancer agents.
Keywords	receptor ; adenosine a1 ; apoptosis ; genes ; casp3 ; t47d cells ; Medicine ; R
Subject code	616 ; 610
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	Hormozgan University of Medical Sciences
Document type	Article ; Online
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