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  1. Book ; Thesis: Definition of molecular pathways with importance for the interaction of leukemic blasts with mesenchymal stem cells

    Fierro, Fernando A.

    2007  

    Author's details von Fernando A. Fierro B
    Language English
    Size 82 Bl., Ill., graph. Darst., 31 cm
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dresden, Techn. Univ., Diss., 2008
    HBZ-ID HT015706007
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2008 E 1401 order for loan Magazin

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  2. Article ; Online: Effects of Spermine Synthase Deficiency in Mesenchymal Stromal Cells Are Rescued by Upstream Inhibition of Ornithine Decarboxylase.

    Cressman, Amin / Morales, David / Zhang, Zhenyang / Le, Bryan / Foley, Jackson / Murray-Stewart, Tracy / Genetos, Damian C / Fierro, Fernando A

    International journal of molecular sciences

    2024  Volume 25, Issue 5

    Abstract: Despite the well-known relevance of polyamines to many forms of life, little is known about how polyamines regulate osteogenesis and skeletal homeostasis. Here, we report a series of in vitro studies conducted with human-bone-marrow-derived pluripotent ... ...

    Abstract Despite the well-known relevance of polyamines to many forms of life, little is known about how polyamines regulate osteogenesis and skeletal homeostasis. Here, we report a series of in vitro studies conducted with human-bone-marrow-derived pluripotent stromal cells (MSCs). First, we show that during osteogenic differentiation, mRNA levels of most polyamine-associated enzymes are relatively constant, except for the catabolic enzyme spermidine/spermine N1-acetyltransferase 1 (SAT1), which is strongly increased at both mRNA and protein levels. As a result, the intracellular spermidine to spermine ratio is significantly reduced during the early stages of osteoblastogenesis. Supplementation of cells with exogenous spermidine or spermine decreases matrix mineralization in a dose-dependent manner. Employing N-cyclohexyl-1,3-propanediamine (CDAP) to chemically inhibit spermine synthase (SMS), the enzyme catalyzing conversion of spermidine into spermine, also suppresses mineralization. Intriguingly, this reduced mineralization is rescued with DFMO, an inhibitor of the upstream polyamine enzyme ornithine decarboxylase (ODC1). Similarly, high concentrations of CDAP cause cytoplasmic vacuolization and alter mitochondrial function, which are also reversible with the addition of DFMO. Altogether, these studies suggest that excess polyamines, especially spermidine, negatively affect hydroxyapatite synthesis of primary MSCs, whereas inhibition of polyamine synthesis with DFMO rescues most, but not all of these defects. These findings are relevant for patients with Snyder-Robinson syndrome (SRS), as the presenting skeletal defects-associated with SMS deficiency-could potentially be ameliorated by treatment with DFMO.
    MeSH term(s) Humans ; Spermidine/metabolism ; Spermine/metabolism ; Spermine Synthase/genetics ; Ornithine Decarboxylase/metabolism ; Osteogenesis ; Polyamines/metabolism ; Mesenchymal Stem Cells/metabolism ; RNA, Messenger
    Chemical Substances Spermidine (U87FK77H25) ; Spermine (2FZ7Y3VOQX) ; Spermine Synthase (EC 2.5.1.22) ; Ornithine Decarboxylase (EC 4.1.1.17) ; Polyamines ; RNA, Messenger
    Language English
    Publishing date 2024-02-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25052463
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Osteochondroma of the Rib: A Potentially Life-Threatening Benign Tumor.

    Morales, Laura C / Cardona Ortegón, Jose D / Pinzón Valderrama, Bibiana A / Jiménez Uribe, Ana M / Mora Bendeck, Nicolas G / Fierro Ávila, Fernando

    Cureus

    2023  Volume 15, Issue 9, Page(s) e45449

    Abstract: Osteochondroma is the most common benign bone tumor. It can be classified as isolated or multiple. While the majority of osteochondromas are asymptomatic and found incidentally, they can become symptomatic during adolescence or adulthood due to ... ...

    Abstract Osteochondroma is the most common benign bone tumor. It can be classified as isolated or multiple. While the majority of osteochondromas are asymptomatic and found incidentally, they can become symptomatic during adolescence or adulthood due to mechanical irritation, nerve compression, spinal cord compression, or vascular injury. In this article, we present a case of a 14-year-old patient who experienced spontaneous hemothorax caused by bleeding from a diaphragmatic laceration incurred by a costal exostosis on the right eighth rib. A preoperative chest CT scan revealed a bony projection from the rib and bloody effusion in the thoracic cavity, highlighting the possibility of bloody pleural effusion due to costal exostosis. It is important to note that costal osteochondromas are a rare cause of thoracic injury and can lead to laceration of the lung, diaphragm, and/or pericardium. Surgical intervention should be considered for symptomatic rib osteochondroma, and we advocate for prophylactic surgical removal of intrathoracic exostosis even in asymptomatic patients, in order to prevent potential complications.
    Language English
    Publishing date 2023-09-18
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.45449
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Reducing Sialylation Enhances Electrotaxis of Corneal Epithelial Cells.

    Le, Bryan / Zhu, Kan / Brown, Chelsea / Reid, Brian / Cressman, Amin / Zhao, Min / Fierro, Fernando A

    International journal of molecular sciences

    2023  Volume 24, Issue 18

    Abstract: Corneal wound healing is a complex biological process that integrates a host of different signals to coordinate cell behavior. Upon wounding, there is the generation of an endogenous wound electric field that serves as a powerful cue to guide cell ... ...

    Abstract Corneal wound healing is a complex biological process that integrates a host of different signals to coordinate cell behavior. Upon wounding, there is the generation of an endogenous wound electric field that serves as a powerful cue to guide cell migration. Concurrently, the corneal epithelium reduces sialylated glycoforms, suggesting that sialylation plays an important role during electrotaxis. Here, we show that pretreating human telomerase-immortalized corneal epithelial (hTCEpi) cells with a sialyltransferase inhibitor, P-3FAX-Neu5Ac (3F-Neu5Ac), improves electrotaxis by enhancing directionality, but not speed. This was recapitulated using Kifunensine, which inhibits cleavage of mannoses and therefore precludes sialylation on N-glycans. We also identified that 3F-Neu5Ac enhanced the responsiveness of the hTCEpi cell population to the electric field and that pretreated hTCEpi cells showed increased directionality even at low voltages. Furthermore, when we increased sialylation using N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz), hTCEpi cells showed a decrease in both speed and directionality. Importantly, pretreating enucleated eyes with 3F-Neu5Ac significantly improved re-epithelialization in an ex vivo model of a corneal injury. Finally, we show that in hTCEpi cells, sialylation is increased by growth factor deprivation and reduced by PDGF-BB. Taken together, our results suggest that during corneal wound healing, reduced sialylated glycoforms enhance electrotaxis and re-epithelialization, potentially opening new avenues to promote corneal wound healing.
    MeSH term(s) Humans ; Cornea ; Epithelium, Corneal/metabolism ; Epithelial Cells/metabolism ; Wound Healing ; Re-Epithelialization ; Corneal Injuries/therapy ; Corneal Injuries/metabolism
    Language English
    Publishing date 2023-09-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241814327
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Reducing Sialylation Enhances Electrotaxis of Corneal Epithelial Cells

    Bryan Le / Kan Zhu / Chelsea Brown / Brian Reid / Amin Cressman / Min Zhao / Fernando A. Fierro

    International Journal of Molecular Sciences, Vol 24, Iss 14327, p

    2023  Volume 14327

    Abstract: Corneal wound healing is a complex biological process that integrates a host of different signals to coordinate cell behavior. Upon wounding, there is the generation of an endogenous wound electric field that serves as a powerful cue to guide cell ... ...

    Abstract Corneal wound healing is a complex biological process that integrates a host of different signals to coordinate cell behavior. Upon wounding, there is the generation of an endogenous wound electric field that serves as a powerful cue to guide cell migration. Concurrently, the corneal epithelium reduces sialylated glycoforms, suggesting that sialylation plays an important role during electrotaxis. Here, we show that pretreating human telomerase-immortalized corneal epithelial (hTCEpi) cells with a sialyltransferase inhibitor, P-3FAX-Neu5Ac (3F-Neu5Ac), improves electrotaxis by enhancing directionality, but not speed. This was recapitulated using Kifunensine, which inhibits cleavage of mannoses and therefore precludes sialylation on N-glycans. We also identified that 3F-Neu5Ac enhanced the responsiveness of the hTCEpi cell population to the electric field and that pretreated hTCEpi cells showed increased directionality even at low voltages. Furthermore, when we increased sialylation using N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz), hTCEpi cells showed a decrease in both speed and directionality. Importantly, pretreating enucleated eyes with 3F-Neu5Ac significantly improved re-epithelialization in an ex vivo model of a corneal injury. Finally, we show that in hTCEpi cells, sialylation is increased by growth factor deprivation and reduced by PDGF-BB. Taken together, our results suggest that during corneal wound healing, reduced sialylated glycoforms enhance electrotaxis and re-epithelialization, potentially opening new avenues to promote corneal wound healing.
    Keywords electrotaxis ; corneal wound healing ; sialic acid ; sialylations ; corneal epithelial cells ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 500 ; 570
    Language English
    Publishing date 2023-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Post-death Vesicles of Senescent Bone Marrow Mesenchymal Stromal Polyploids Promote Macrophage Aging and Breast Cancer.

    Xie, Bowen / Fan, Ming / Wang, Charles X / Zhang, Yanhong / Xu, Shanxiu / Mizenko, Rachel / Lin, Tzu-Yin / Duan, Yixin / Zhang, Yanyan / Huang, Jie / Berg, Jonathan I / Wu, Douglas / Li, Anna / Hao, Dake / Gao, Kewa / Sun, Yaohui / Tepper, Clifford G / Carney, Randy / Li, Yuanpei /
    Wang, Aijun / Gong, Qizhi / Daly, Magen / Jao, Li-En / Monjazeb, Arta M / Fierro, Fernando A / Li, Jian Jian

    bioRxiv : the preprint server for biology

    2024  

    Language English
    Publishing date 2024-03-08
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.06.583755
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Antifibrotic Drugs against Idiopathic Pulmonary Fibrosis and Pulmonary Fibrosis Induced by COVID-19: Therapeutic Approaches and Potential Diagnostic Biomarkers.

    Perez-Favila, Aurelio / Garza-Veloz, Idalia / Hernandez-Marquez, Lucia Del Socorro / Gutierrez-Vela, Edgar Fernando / Flores-Morales, Virginia / Martinez-Fierro, Margarita L

    International journal of molecular sciences

    2024  Volume 25, Issue 3

    Abstract: The COVID-19 pandemic has had a significant impact on the health and economy of the global population. Even after recovery from the disease, post-COVID-19 symptoms, such as pulmonary fibrosis, continue to be a concern. This narrative review aims to ... ...

    Abstract The COVID-19 pandemic has had a significant impact on the health and economy of the global population. Even after recovery from the disease, post-COVID-19 symptoms, such as pulmonary fibrosis, continue to be a concern. This narrative review aims to address pulmonary fibrosis (PF) from various perspectives, including the fibrotic mechanisms involved in idiopathic and COVID-19-induced pulmonary fibrosis. On the other hand, we also discuss the current therapeutic drugs in use, as well as those undergoing clinical or preclinical evaluation. Additionally, this article will address various biomarkers with usefulness for PF prediction, diagnosis, treatment, prognosis, and severity assessment in order to provide better treatment strategies for patients with this disease.
    MeSH term(s) Humans ; Pandemics ; COVID-19/complications ; Idiopathic Pulmonary Fibrosis/diagnosis ; Idiopathic Pulmonary Fibrosis/drug therapy ; Idiopathic Pulmonary Fibrosis/etiology ; Fibrosis ; Biomarkers ; COVID-19 Testing
    Chemical Substances Biomarkers
    Language English
    Publishing date 2024-01-26
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25031562
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: An immune deficient mouse model for mucopolysaccharidosis IIIA (Sanfilippo syndrome).

    Pollock, Kari / Noritake, Sabrina / Imai, Denise M / Pastenkos, Gabrielle / Olson, Marykate / Cary, Whitney / Yang, Sheng / Fierro, Fernando A / White, Jeannine / Graham, Justin / Dahlenburg, Heather / Johe, Karl / Nolta, Jan A

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 18439

    Abstract: Mucopolysaccharidosis III (MPSIII, Sanfilippo syndrome) is a devastating lysosomal storage disease that primarily affects the central nervous system. MPSIIIA is caused by loss-of-function mutations in the gene coding for sulfamidase (N-sulfoglucosamine ... ...

    Abstract Mucopolysaccharidosis III (MPSIII, Sanfilippo syndrome) is a devastating lysosomal storage disease that primarily affects the central nervous system. MPSIIIA is caused by loss-of-function mutations in the gene coding for sulfamidase (N-sulfoglucosamine sulfohydrolase/SGSH) resulting in SGSH enzyme deficiency, a buildup of heparin sulfate and subsequent neurodegeneration. There is currently no cure or disease modifying treatment for MPSIIIA. A mouse model for MPSIIIA was characterized in 1999 and later backcrossed onto the C57BL/6 background. In the present study, a novel immune deficient MPSIIIA mouse model (MPSIIIA-TKO) was created by backcrossing the immune competent, C57BL/6 MPSIIIA mouse to an immune deficient mouse model lacking Rag2, CD47 and Il2rg genes. The resulting mouse model has undetectable SGSH activity, exhibits histological changes consistent with MPSIIIA and lacks T cells, B cells and NK cells. This new mouse model has the potential to be extremely useful in testing human cellular therapies in an animal model as it retains the MPSIIIA disease phenotype while tolerating xenotransplantation.
    MeSH term(s) Animals ; Humans ; Mice ; Mucopolysaccharidosis III/genetics ; Mucopolysaccharidosis III/pathology ; Mice, Inbred C57BL ; Hydrolases/genetics ; Phenotype ; Disease Models, Animal
    Chemical Substances Hydrolases (EC 3.-)
    Language English
    Publishing date 2023-10-27
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-45178-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: An immune deficient mouse model for mucopolysaccharidosis IIIA (Sanfilippo syndrome)

    Kari Pollock / Sabrina Noritake / Denise M. Imai / Gabrielle Pastenkos / Marykate Olson / Whitney Cary / Sheng Yang / Fernando A. Fierro / Jeannine White / Justin Graham / Heather Dahlenburg / Karl Johe / Jan A. Nolta

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Volume 11

    Abstract: Abstract Mucopolysaccharidosis III (MPSIII, Sanfilippo syndrome) is a devastating lysosomal storage disease that primarily affects the central nervous system. MPSIIIA is caused by loss-of-function mutations in the gene coding for sulfamidase (N- ... ...

    Abstract Abstract Mucopolysaccharidosis III (MPSIII, Sanfilippo syndrome) is a devastating lysosomal storage disease that primarily affects the central nervous system. MPSIIIA is caused by loss-of-function mutations in the gene coding for sulfamidase (N-sulfoglucosamine sulfohydrolase/SGSH) resulting in SGSH enzyme deficiency, a buildup of heparin sulfate and subsequent neurodegeneration. There is currently no cure or disease modifying treatment for MPSIIIA. A mouse model for MPSIIIA was characterized in 1999 and later backcrossed onto the C57BL/6 background. In the present study, a novel immune deficient MPSIIIA mouse model (MPSIIIA-TKO) was created by backcrossing the immune competent, C57BL/6 MPSIIIA mouse to an immune deficient mouse model lacking Rag2, CD47 and Il2rg genes. The resulting mouse model has undetectable SGSH activity, exhibits histological changes consistent with MPSIIIA and lacks T cells, B cells and NK cells. This new mouse model has the potential to be extremely useful in testing human cellular therapies in an animal model as it retains the MPSIIIA disease phenotype while tolerating xenotransplantation.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2023-10-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Concise Review: Stem Cells in Osteoimmunology.

    Fierro, Fernando A / Nolta, Jan A / Adamopoulos, Iannis E

    Stem cells (Dayton, Ohio)

    2017  Volume 35, Issue 6, Page(s) 1461–1467

    Abstract: Bone remodeling is a lifelong process in which mature bone tissue is removed from the skeleton by bone resorption and is replenished by new during ossification or bone formation. The remodeling cycle requires both the differentiation and activation of ... ...

    Abstract Bone remodeling is a lifelong process in which mature bone tissue is removed from the skeleton by bone resorption and is replenished by new during ossification or bone formation. The remodeling cycle requires both the differentiation and activation of two cell types with opposing functions; the osteoclast, which orchestrates bone resorption, and the osteoblast, which orchestrates bone formation. The differentiation of these cells from their respective precursors is a process which has been overshadowed by enigma, particularly because the precise osteoclast precursor has not been identified and because the identification of skeletal stem cells, which give rise to osteoblasts, is very recent. Latest advances in the area of stem cell biology have enabled us to gain a better understanding of how these differentiation processes occur in physiological and pathological conditions. In this review we postulate that modulation of stem cells during inflammatory conditions is a necessary prerequisite of bone remodeling and therefore an essential new component to the field of osteoimmunology. In this context, we highlight the role of transcription factor nuclear factor of activated T cells cytoplasmic 1 (NFATc1), because it directly links inflammation with differentiation of osteoclasts and osteoblasts. Stem Cells 2017;35:1461-1467.
    MeSH term(s) Animals ; Bone Resorption ; Bone and Bones/cytology ; Bone and Bones/immunology ; Humans ; Models, Biological ; Osteoclasts/cytology ; Osteogenesis ; Stem Cells/cytology
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 1143556-2
    ISSN 1549-4918 ; 1066-5099
    ISSN (online) 1549-4918
    ISSN 1066-5099
    DOI 10.1002/stem.2625
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    In stock of ZB MED Cologne/Königswinter

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    Jg. 1995 - 2021: Lesesall (1.OG)
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