Article ; Online: Determinación de poblaciones circulantes en sangre periférica de linfocitos T, reguladores CD4
Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993)
2024 Volume 71, Issue 1, Page(s) 73
Abstract: ... Tregs was 11% of all CD4+ T cells, with a range of minimum 8,1% and maximum 17,7%. There was no ... the percentage of regulatory T cells using cell surface markers. ...
Title translation | Circulating populations of CD4+ CD25+ CD127- regulatory t lymphocytes in peripheral blood of allergic asthmatic children. |
---|---|
Abstract | Objective: To carry out a preliminary analysis on the Treg lymphocyte counts present in the peripheral blood of allergic asthmatic children from the city of Cartagena, Colombia, compared to healthy controls. Methods: We compared cytometry counts of ten asthmatic patients (age 7-16 years) and seven healthy controls (6-12 years), recruited in the city of Cartagena. Peripheral blood samples were stained using Cytek's 14-color cFluor Immunoprofiling kit (Cytek Results: The frequency of CD3+, CD4+, CD25+, CD127- Tregs was 11% of all CD4+ T cells, with a range of minimum 8,1% and maximum 17,7%. There was no significant difference in the proportion of Tregs between allergic asthmatic patients and healthy controls (P = 0,2). Conclusions: With this preliminary sample size, no significant differences were found in the Treg lymphocyte population between allergic asthmatic patients and healthy controls. The 14-color multiplexed panel is a useful tool not only to count CD3+ and CD4+ populations, but also to obtain the percentage of regulatory T cells using cell surface markers. |
MeSH term(s) | Humans ; Child ; Asthma/blood ; Asthma/immunology ; T-Lymphocytes, Regulatory/immunology ; Adolescent ; Male ; Female ; Interleukin-2 Receptor alpha Subunit/blood ; Interleukin-2 Receptor alpha Subunit/analysis ; Interleukin-7 Receptor alpha Subunit/analysis ; Interleukin-7 Receptor alpha Subunit/blood ; CD4 Antigens/analysis ; CD4 Antigens/blood ; Lymphocyte Count |
Chemical Substances | Interleukin-2 Receptor alpha Subunit ; Interleukin-7 Receptor alpha Subunit ; CD4 Antigens |
Language | Spanish |
Publishing date | 2024-02-01 |
Publishing country | Mexico |
Document type | English Abstract ; Journal Article |
ZDB-ID | 639125-4 |
ISSN | 2448-9190 ; 0002-5151 |
ISSN (online) | 2448-9190 |
ISSN | 0002-5151 |
DOI | 10.29262/ram.v71i1.1363 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
Zs.B 488: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.