Article: RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence Notch target genes.
Molecular and cellular biology
2005 Volume 25, Issue 23, Page(s) 10379–10390
Abstract: Notch is a transmembrane receptor that determines cell fates and pattern formation in all animal species. After ligand binding, proteolytic cleavage steps occur and the intracellular part of Notch translocates to the nucleus, where it targets the DNA- ... ...
| Abstract | Notch is a transmembrane receptor that determines cell fates and pattern formation in all animal species. After ligand binding, proteolytic cleavage steps occur and the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. We and others have identified SHARP as a component of this complex. Here, we functionally demonstrate that the SHARP repression domain is necessary and sufficient to repress transcription and that the absence of this domain causes a dominant negative Notch-like phenotype. We identify the CtIP and CtBP corepressors as novel components of the human RBP-Jkappa/SHARP-corepressor complex and show that CtIP binds directly to the SHARP repression domain. Functionally, CtIP and CtBP augment SHARP-mediated repression. Transcriptional repression of the Notch target gene Hey1 is abolished in CtBP-deficient cells or after the functional knockout of CtBP. Furthermore, the endogenous Hey1 promoter is derepressed in CtBP-deficient cells. We propose that a corepressor complex containing CtIP/CtBP facilitates RBP-Jkappa/SHARP-mediated repression of Notch target genes. |
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| MeSH term(s) | Alcohol Oxidoreductases ; Animals ; Basic Helix-Loop-Helix Transcription Factors/chemistry ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Basic Helix-Loop-Helix Transcription Factors/metabolism ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Cell Line ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Embryo, Nonmammalian/embryology ; Embryo, Nonmammalian/metabolism ; Gene Silencing ; Humans ; Immunoglobulin J Recombination Signal Sequence-Binding Protein/genetics ; Immunoglobulin J Recombination Signal Sequence-Binding Protein/metabolism ; Mice ; Multiprotein Complexes/chemistry ; Multiprotein Complexes/metabolism ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism ; Phosphoproteins/genetics ; Phosphoproteins/metabolism ; Receptors, Notch/genetics ; Receptors, Notch/metabolism ; Repressor Proteins/genetics ; Repressor Proteins/metabolism ; Xenopus laevis/embryology ; Xenopus laevis/genetics ; Xenopus laevis/metabolism |
| Chemical Substances | BHLHE41 protein, human ; Basic Helix-Loop-Helix Transcription Factors ; Carrier Proteins ; DNA-Binding Proteins ; Immunoglobulin J Recombination Signal Sequence-Binding Protein ; Multiprotein Complexes ; Nuclear Proteins ; Phosphoproteins ; RBPJ protein, human ; Receptors, Notch ; Repressor Proteins ; Alcohol Oxidoreductases (EC 1.1.-) ; C-terminal binding protein (EC 1.1.1.-) ; RBBP8 protein, human (EC 3.1.-) |
| Language | English |
| Publishing date | 2005-10-06 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 779397-2 |
| ISSN | 1098-5549 ; 0270-7306 |
| ISSN (online) | 1098-5549 |
| ISSN | 0270-7306 |
| DOI | 10.1128/MCB.25.23.10379-10390.2005 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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