LIVIVO - Search results -

Search results

Result 1 - 10 of total 11546

Search options

Article ; Online: Usefulness of the FilmArray meningitis/encephalitis (M/E) panel for the diagnosis of infectious meningitis and encephalitis in Taiwan.

Lee, Sze Hwei / Chen, Shey-Ying / Chien, Jung-Yien / Lee, Tai-Fen / Chen, Jong-Min / Hsueh, Po-Ren

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi

2019 Volume 52, Issue 5, Page(s) 760–768

Abstract: Background/purpose: Early recognition of causative pathogens is critical for the appropriate management of central nervous system infection and improved outcomes. The BioFire: Methods: In this preliminary study, we used the BioFire: Results: The ... ...

Abstract	Background/purpose: Early recognition of causative pathogens is critical for the appropriate management of central nervous system infection and improved outcomes. The BioFire Methods: In this preliminary study, we used the BioFire Results: The panel detected six positive samples, of which five were viral and one bacterial. We observed an overall agreement rate of 88% between the BioFire Conclusions: The BioFire
MeSH term(s)	Adult ; Aged ; Bacteria/isolation & purification ; Diagnostic Tests, Routine/instrumentation ; Diagnostic Tests, Routine/methods ; Emergency Service, Hospital ; Encephalitis/diagnosis ; Female ; Fungi/isolation & purification ; Hospitals, University ; Humans ; Male ; Meningitis/diagnosis ; Middle Aged ; Multiplex Polymerase Chain Reaction/methods ; Prevalence ; Prospective Studies ; Taiwan ; Viruses/isolation & purification
Language	English
Publishing date	2019-04-30
Publishing country	England
Document type	Journal Article
ZDB-ID	1497590-7
ISSN	1995-9133 ; 1684-1182 ; 0253-2662
ISSN (online)	1995-9133
ISSN	1684-1182 ; 0253-2662
DOI	10.1016/j.jmii.2019.04.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 2083: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: Prokaryotic soluble overexpression and purification of oncostatin M using a fusion approach and genetically engineered E. coli strains.

Nguyen, Minh Tan / Prima, Musharrat Jahan / Song, Jung-A / Kim, Julee / Do, Bich Hang / Yoo, Jiwon / Park, Sangsu / Jang, Jaepyeong / Lee, Sunju / Lee, Eunyoung / Novais, Michelle de Paula / Seo, Hyeon-Beom / Lee, Seon-Yeong / Cho, Mi-La / Kim, Chong Jai / Jang, Yeon Jin / Choe, Han

Scientific reports

2019 Volume 9, Issue 1, Page(s) 13706

Abstract: Human Oncostatin M (OSM), initially discovered as a tumour inhibitory factor secreted from U-937 ... the b'a' domain of PDI (PDIb'a'), were tested for soluble OSM expression in E. coli. The His6-OSM plasmid ...

Abstract	Human Oncostatin M (OSM), initially discovered as a tumour inhibitory factor secreted from U-937 cells, is a gp130 (IL-6/LIF) cytokine family member that exhibits pleiotropic effects in inflammation, haematopoiesis, skeletal tissue alteration, liver regeneration, cardiovascular and metabolic diseases. Cytoplasmic expression of OSM in Escherichia coli results in inclusion bodies, and complex solubilisation, refolding and purification is required to prepare bioactive protein. Herein, eight N-terminal fusion variants of OSM with hexahistidine (His6) tag and seven solubility-enhancing tags, including thioredoxin (Trx), small ubiquitin-related modifier (Sumo), glutathione S-transferase (GST), maltose-binding protein (MBP), N-utilisation substance protein A (Nusa), human protein disulphide isomerase (PDI) and the b'a' domain of PDI (PDIb'a'), were tested for soluble OSM expression in E. coli. The His6-OSM plasmid was also introduced into genetically engineered Origami 2 and SHuffle strains to test expression of the protein. At 18 °C, MBP-tagged OSM was highly expressed and solubility was dramatically enhanced. In addition, His6-OSM was more highly expressed and soluble in Origami 2 and SHuffle strains than in BL21(DE3). MBP-OSM and His6-OSM were purified more than 95% with yields of 11.02 mg and 3.27 mg from a 500 mL culture. Protein identity was confirmed by mass spectroscopy, and bioactivity was demonstrated by in vitro inhibition of Th17 cell differentiation.
MeSH term(s)	Escherichia coli ; Gene Expression ; Genetic Engineering ; Histidine ; Humans ; Maltose-Binding Proteins/metabolism ; Oligopeptides ; Oncostatin M/genetics ; Oncostatin M/metabolism ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Solubility
Chemical Substances	His-His-His-His-His-His ; Maltose-Binding Proteins ; Oligopeptides ; Recombinant Fusion Proteins ; Oncostatin M (106956-32-5) ; Histidine (4QD397987E)
Language	English
Publishing date	2019-09-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-50110-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Prokaryotic soluble overexpression and purification of oncostatin M using a fusion approach and genetically engineered E. coli strains

Minh Tan Nguyen / Musharrat Jahan Prima / Jung-A. Song / Julee Kim / Bich Hang Do / Jiwon Yoo / Sangsu Park / Jaepyeong Jang / Sunju Lee / Eunyoung Lee / Michelle de Paula Novais / Hyeon-Beom Seo / Seon-yeong Lee / Mi-La Cho / Chong Jai Kim / Yeon Jin Jang / Han Choe

Scientific Reports, Vol 9, Iss 1, Pp 1-

2019 Volume 13

Abstract: Abstract Human Oncostatin M (OSM), initially discovered as a tumour inhibitory factor secreted ... the b‘a’ domain of PDI (PDIb‘a’), were tested for soluble OSM expression in E. coli. The His6-OSM ...

Abstract	Abstract Human Oncostatin M (OSM), initially discovered as a tumour inhibitory factor secreted from U-937 cells, is a gp130 (IL-6/LIF) cytokine family member that exhibits pleiotropic effects in inflammation, haematopoiesis, skeletal tissue alteration, liver regeneration, cardiovascular and metabolic diseases. Cytoplasmic expression of OSM in Escherichia coli results in inclusion bodies, and complex solubilisation, refolding and purification is required to prepare bioactive protein. Herein, eight N-terminal fusion variants of OSM with hexahistidine (His6) tag and seven solubility-enhancing tags, including thioredoxin (Trx), small ubiquitin-related modifier (Sumo), glutathione S-transferase (GST), maltose-binding protein (MBP), N-utilisation substance protein A (Nusa), human protein disulphide isomerase (PDI) and the b‘a’ domain of PDI (PDIb‘a’), were tested for soluble OSM expression in E. coli. The His6-OSM plasmid was also introduced into genetically engineered Origami 2 and SHuffle strains to test expression of the protein. At 18 °C, MBP-tagged OSM was highly expressed and solubility was dramatically enhanced. In addition, His6-OSM was more highly expressed and soluble in Origami 2 and SHuffle strains than in BL21(DE3). MBP-OSM and His6-OSM were purified more than 95% with yields of 11.02 mg and 3.27 mg from a 500 mL culture. Protein identity was confirmed by mass spectroscopy, and bioactivity was demonstrated by in vitro inhibition of Th17 cell differentiation.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2019-09-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-κB.

Soh, Hendrick / Venkatesan, Natarajan / Veena, Mysore S / Ravichandran, Sandhiya / Zinabadi, Alborz / Basak, Saroj K / Parvatiyar, Kislay / Srivastava, Meera / Liang, Li-Jung / Gjertson, David W / Torres, Jorge Z / Moatamed, Neda A / Srivatsan, Eri S

Molecular and cellular biology

2016 Volume 36, Issue 12, Page(s) 1776–1792

Abstract: We and others have shown that the cystatin E/M gene is inactivated in primary human tumors ... not yet understood. Using plasmid-directed cystatin E/M gene overexpression, a lentivirus-mediated ... of tumor necrosis factor alpha (TNF-α), confirming the role of cystatin E/M in the regulation of the NF-κB signaling pathway ...

Abstract	We and others have shown that the cystatin E/M gene is inactivated in primary human tumors, pointing to its role as a tumor suppressor gene. However, the molecular mechanism of tumor suppression is not yet understood. Using plasmid-directed cystatin E/M gene overexpression, a lentivirus-mediated tetracycline-inducible vector system, and human papillomavirus 16 (HPV 16) E6 and E7 gene-immortalized normal human epidermal keratinocytes, we demonstrated intracellular and non-cell-autonomous apoptotic growth inhibition of tumor cell lines and that growth inhibition is associated with cytoplasmic retention of NF-κB. We further demonstrated decreased phosphorylation of IκB kinase (IKKβ) and IκBα in the presence of tumor necrosis factor alpha (TNF-α), confirming the role of cystatin E/M in the regulation of the NF-κB signaling pathway. Growth suppression of nude mouse xenograft tumors carrying a tetracycline-inducible vector system was observed with the addition of doxycycline in drinking water, confirming that the cystatin E/M gene is a tumor suppressor gene. Finally, immunohistochemical analyses of cervical carcinoma in situ and primary tumors have shown a statistically significant inverse relationship between the expression of cystatin E/M and cathepsin L and a direct relationship between the loss of cystatin E/M expression and nuclear expression of NF-κB. We therefore propose that the cystatin E/M suppressor gene plays an important role in the regulation of NF-κB.
MeSH term(s)	Animals ; Cathepsin L/metabolism ; Cell Line, Tumor ; Cell Proliferation ; Cystatin M/genetics ; Cystatin M/metabolism ; Cytoplasm/metabolism ; Doxycycline/administration & dosage ; Female ; Gene Expression Regulation, Neoplastic ; Genetic Vectors/pharmacology ; HeLa Cells ; Humans ; I-kappa B Proteins/metabolism ; Lentivirus/genetics ; Mice ; Mice, Nude ; NF-kappa B/metabolism ; Neoplasm Transplantation ; Phosphorylation ; Signal Transduction ; Tumor Necrosis Factor-alpha/metabolism ; Uterine Cervical Neoplasms/genetics ; Uterine Cervical Neoplasms/metabolism ; Uterine Cervical Neoplasms/pathology
Chemical Substances	CST6 protein, human ; Cystatin M ; I-kappa B Proteins ; NF-kappa B ; TNF protein, human ; Tumor Necrosis Factor-alpha ; CTSL protein, human (EC 3.4.22.15) ; Cathepsin L (EC 3.4.22.15) ; Doxycycline (N12000U13O)
Language	English
Publishing date	2016-05-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	779397-2
ISSN	1098-5549 ; 0270-7306
ISSN (online)	1098-5549
ISSN	0270-7306
DOI	10.1128/MCB.00878-15
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1666: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-κB

Soh, Hendrick / Venkatesan, Natarajan / Veena, Mysore S. / Ravichandran, Sandhiya / Zinabadi, Alborz / Basak, Saroj K. / Parvatiyar, Kislay / Srivastava, Meera / Liang, Li-Jung / Gjertson, David W. / Torres, Jorge Z. / Moatamed, Neda A. / Srivatsan, Eri S.

Molecular and Cellular Biology. 2016 June 1, v. 36, no. 12 p.1776-1792

2016

Abstract	We and others have shown that the cystatin E/M gene is inactivated in primary human tumors, pointing to its role as a tumor suppressor gene. However, the molecular mechanism of tumor suppression is not yet understood. Using plasmid-directed cystatin E/M gene overexpression, a lentivirus-mediated tetracycline-inducible vector system, and human papillomavirus 16 (HPV 16) E6 and E7 gene-immortalized normal human epidermal keratinocytes, we demonstrated intracellular and non-cell-autonomous apoptotic growth inhibition of tumor cell lines and that growth inhibition is associated with cytoplasmic retention of NF-κB. We further demonstrated decreased phosphorylation of IκB kinase (IKKβ) and IκBα in the presence of tumor necrosis factor alpha (TNF-α), confirming the role of cystatin E/M in the regulation of the NF-κB signaling pathway. Growth suppression of nude mouse xenograft tumors carrying a tetracycline-inducible vector system was observed with the addition of doxycycline in drinking water, confirming that the cystatin E/M gene is a tumor suppressor gene. Finally, immunohistochemical analyses of cervical carcinoma in situ and primary tumors have shown a statistically significant inverse relationship between the expression of cystatin E/M and cathepsin L and a direct relationship between the loss of cystatin E/M expression and nuclear expression of NF-κB. We therefore propose that the cystatin E/M suppressor gene plays an important role in the regulation of NF-κB.
Keywords	Human papillomavirus type 16 ; apoptosis ; cathepsin L ; cell growth ; doxycycline ; gene overexpression ; growth retardation ; humans ; immunohistochemistry ; keratinocytes ; mice ; neoplasm cells ; phosphorylation ; suppressor genes ; tumor necrosis factor-alpha ; tumor suppressor genes ; uterine cervical neoplasms ; xenotransplantation
Language	English
Dates of publication	2016-0601
Size	p. 1776-1792.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	779397-2
ISSN	1098-5549 ; 0270-7306
ISSN (online)	1098-5549
ISSN	0270-7306
DOI	10.1128/MCB.00878-15
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 1666: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Resveratrol analogue (E)-8-acetoxy-2-[2-(3,4-diacetoxyphenyl)ethenyl]-quinazoline induces G₂/M cell cycle arrest through the activation of ATM/ATR in human cervical carcinoma HeLa cells.

Kim, Jong-Yun / Choi, Hye-Eun / Lee, Hwi-Ho / Shin, Ji-Sun / Shin, Dong-Hyun / Choi, Jung-Hye / Lee, Yong Sup / Lee, Kyung-Tae

Oncology reports

2015 Volume 33, Issue 5, Page(s) 2639–2647

Abstract: ... cytotoxic effects of various styrylquinazoline derivatives and found that (E)-8-acetoxy-2-[2-(3,4 ... a cell cycle arrest at the G₂/M phase by DNA flow cytometric analysis, which was accompanied by upregulation of cyclin ... p53- or ATM/ATR-specific siRNA significantly attenuated 8-ADEQ-induced G₂/M arrest. These results ...

Abstract	Styrylquinazolines are synthetic analogues of resveratrol and have been suggested to cause anti-inflammatory activity by modulating prostaglandin E₂ (PGE₂) production. In the present study, we evaluated cytotoxic effects of various styrylquinazoline derivatives and found that (E)-8-acetoxy-2-[2-(3,4-diacetoxyphenyl)ethenyl]-quinazoline (8-ADEQ) most potently inhibited the proliferation of the human cervical carcinoma HeLa cells. Exploring the growth-inhibitory mechanisms of 8-ADEQ, we found that it causes a cell cycle arrest at the G₂/M phase by DNA flow cytometric analysis, which was accompanied by upregulation of cyclin B1 expression and cyclin-dependent protein kinase 1 (Cdk1) phosphorylation. In addition, we observed that 8-ADEQ causes phosphorylation of the cell division cycle 25C (Cdc25C) protein through the activation of checkpoint kinases 1 (Chk1) and Chk2, which in turn were activated via ataxia telangiectasia mutated (ATM)/ataxia telangiectasia-Rad3-related (ATR) kinases in response to the DNA damage. Furthermore, ATM/ATR inhibitor caffeine, p53- or ATM/ATR-specific siRNA significantly attenuated 8-ADEQ-induced G₂/M arrest. These results suggest that the 8-ADEQ inhibits the proliferation of human cervical cancer HeLa cells by DNA damage-mediated G₂/M cell cycle arrest. 8-ADEQ‑induced G₂/M arrest is mediated by the activation of both Chk1/2-Cdc25 and p53-p21CIP1/WAF1 via ATM/ATR pathway, and indicates that 8-ADEQ appears to have potential in the treatment of cervical cancer.
MeSH term(s)	Ataxia Telangiectasia Mutated Proteins/genetics ; CDC2 Protein Kinase ; Carcinoma/genetics ; Cell Cycle Checkpoints/drug effects ; Cell Cycle Checkpoints/genetics ; Cell Division/drug effects ; Cell Division/genetics ; Checkpoint Kinase 1 ; Checkpoint Kinase 2/genetics ; Cyclin B1/genetics ; Cyclin-Dependent Kinase Inhibitor p21 ; Cyclin-Dependent Kinases/genetics ; DNA Damage/drug effects ; DNA Damage/genetics ; Female ; G2 Phase/drug effects ; G2 Phase/genetics ; HeLa Cells ; Humans ; Phosphorylation/drug effects ; Phosphorylation/genetics ; Protein Kinases/genetics ; Quinazolines/pharmacology ; Signal Transduction/drug effects ; Signal Transduction/genetics ; Stilbenes/pharmacology ; Tumor Suppressor Protein p53/drug effects ; Tumor Suppressor Protein p53/genetics ; Up-Regulation/drug effects ; Up-Regulation/genetics ; Uterine Cervical Neoplasms/genetics ; cdc25 Phosphatases/genetics
Chemical Substances	CDKN1A protein, human ; Cyclin B1 ; Cyclin-Dependent Kinase Inhibitor p21 ; Quinazolines ; Stilbenes ; TP53 protein, human ; Tumor Suppressor Protein p53 ; Protein Kinases (EC 2.7.-) ; Checkpoint Kinase 2 (EC 2.7.1.11) ; ATM protein, human (EC 2.7.11.1) ; ATR protein, human (EC 2.7.11.1) ; Ataxia Telangiectasia Mutated Proteins (EC 2.7.11.1) ; CHEK1 protein, human (EC 2.7.11.1) ; CHEK2 protein, human (EC 2.7.11.1) ; Checkpoint Kinase 1 (EC 2.7.11.1) ; CDC2 Protein Kinase (EC 2.7.11.22) ; CDK1 protein, human (EC 2.7.11.22) ; Cyclin-Dependent Kinases (EC 2.7.11.22) ; CDC25C protein, human (EC 3.1.3.48) ; cdc25 Phosphatases (EC 3.1.3.48) ; resveratrol (Q369O8926L)
Language	English
Publishing date	2015-05
Publishing country	Greece
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1222484-4
ISSN	1791-2431 ; 1021-335X
ISSN (online)	1791-2431
ISSN	1021-335X
DOI	10.3892/or.2015.3871
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4213: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Molecular characterization of HE, M, and E genes of winter dysentery bovine coronavirus circulated in Korea during 2002-2003.

Ko, Chin-Koo / Kang, Mun-Il / Lim, Geum-Ki / Kim, Gye-Yeop / Yoon, Soon-Seek / Park, Jong-Tae / Jeong, Cheol / Park, Sung-Hee / Park, Su-Jin / Kim, You-Jung / Jeong, Jae-Ho / Kim, Sang-Ki / Park, Sang-Ilk / Kim, Ha-Hyun / Kim, Kyoung-Yoon / Cho, Kyoung-Oh

Virus genes

2006 Volume 32, Issue 2, Page(s) 129–136

Abstract: ... esterase (HE) protein, the transmembrane (M) protein and the small membrane (E) protein to characterize 10 ... Korean WD strains had evolutionary distinct pathway. In contrast, the relative conservation of the M and ... E proteins of BCoV including Korean WD strains and the other coronaviruses suggested that structural ...

Abstract	The different bovine coronavirus (BCoV) strains or isolates exhibited various degrees of substitutions, resulting in altered antigenicity and pathogenicity of the virus. In the previous our study, we demonstrated that the spike glycoprotein gene of Korean winter dysentery (WD) BCoV had a genetic property of both enteric (EBCV) and respiratory BCoV (RBCV) and were significantly distinct from the ancestral enteric strains. In the present study, therefore, we analyzed the other structure genes, the hemagglutinin/esterase (HE) protein, the transmembrane (M) protein and the small membrane (E) protein to characterize 10 WD BCoV circulated in Korea during 2002-2003 and compared the nucleotide and deduced amino acid sequences with the other known BCoV. Phylogenetic analysis indicated that the HE gene among BCoV could be divided into three groups. The first group included only RBCV, while the second group contained calf diarrhea BCoV, RBCV, WD and EBCV, respectively. The third group possessed only all Korean WD strains which were more homologous to each other and were sharply distinct from the other known BCoV, suggesting Korean WD strains had evolutionary distinct pathway. In contrast, the relative conservation of the M and E proteins of BCoV including Korean WD strains and the other coronaviruses suggested that structural constraints on these proteins are rigid, resulting in more limited evolution of these proteins. In addition, BCoV and human coronavirus HCV-OC43 contained four potential O-glycosylation sites in the M gene. However, the M gene sequence of both BCoV and HCV-OC43 might not contain a signal peptide, suggesting the M protein might be unlikely to be exposed to the O-glycosylation machinery in vivo.
MeSH term(s)	Animals ; Cattle ; Cattle Diseases/virology ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Coronavirus M Proteins ; Coronavirus OC43, Human/genetics ; Coronavirus, Bovine/classification ; Coronavirus, Bovine/genetics ; Coronavirus, Bovine/isolation & purification ; Dysentery/veterinary ; Dysentery/virology ; Evolution, Molecular ; Glycosylation ; Hemagglutinins, Viral/genetics ; Korea ; Molecular Sequence Data ; Phylogeny ; Protein Sorting Signals/genetics ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; Viral Envelope Proteins/genetics ; Viral Fusion Proteins/genetics ; Viral Matrix Proteins/genetics
Chemical Substances	Coronavirus M Proteins ; Hemagglutinins, Viral ; M protein, Human coronavirus OC43 ; Protein Sorting Signals ; Viral Envelope Proteins ; Viral Fusion Proteins ; Viral Matrix Proteins ; hemagglutinin esterase
Keywords	covid19
Language	English
Publishing date	2006-01-18
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639496-6
ISSN	1572-994X ; 0920-8569
ISSN (online)	1572-994X
ISSN	0920-8569
DOI	10.1007/s11262-005-6867-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2397: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Identification of antigenic proteins from Neospora caninum recognized by bovine immunoglobulins M, E, A and G using immunoproteomics.

Shin, Yong-seung / Lee, Eung-goo / Shin, Gee-wook / Kim, Young-rim / Lee, Eun-young / Kim, Jae-hoon / Jang, Hwan / Gershwin, Laurel J / Kim, Dae-yong / Kim, Yong-hwan / Kim, Gon-sup / Suh, Myung-deuk / Jung, Tae-sung

Proteomics

2004 Volume 4, Issue 11, Page(s) 3600–3609

Abstract: Antigenic proteins of Neospora caninum (N. caninum) against bovine immunoglobulins M, E, A, and G ...

Abstract	Antigenic proteins of Neospora caninum (N. caninum) against bovine immunoglobulins M, E, A, and G were investigated by using immunoproteomics. Proteins of N. caninum (KBA-2) tachyzoite lysates separated by two-dimensional gel electrophoresis were transferred to polyvinylidene difluoride (PVDF) membranes, probed with different bovine immunoglobulin class and classified. Antigenic spots recognized were also identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis. 132, 84, 4, and 40 antigenic protein spots were recognized on N. caninum immunoblot profiles against bovine IgM, IgE, IgA, and IgG, respectively. Of these protein spots, the antigenic proteins recognized by either IgM, IgE, and IgG, or IgM and IgG were HSP70, pyruvate kinase, actin, NCDG-1, tubulin alpha-chain, and putative ribosomal protein S2. On the other hand, IgM, IgE, and IgA reacted with NTPase, HSP60, tubulin beta-chain, putative protein disulfide isomerase, enolase, lactate dehydrogenase, serine-threonine phosphatase, 14-3-3 protein homologue, and GRA2 protein. Most of the antigenic proteins identified were associated with the process of invasion, proliferation, and egression of apicomplexans. In our study, HSP70, actin, NTPase, HSP60, pyruvate kinase, enolase, putative ribosomal protein S2, NCDG-1, and GRA2 proteins were found to be immunodominant proteins, which may contribute to the development of diagnostic markers and vaccine.
MeSH term(s)	Animals ; Antigens, Protozoan/immunology ; Cattle ; Electrophoresis, Gel, Two-Dimensional ; Immunoblotting ; Immunoglobulins/immunology ; Neospora/immunology ; Proteome/immunology
Chemical Substances	Antigens, Protozoan ; Immunoglobulins ; Proteome
Language	English
Publishing date	2004-11
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2032093-0
ISSN	1615-9861 ; 1615-9853
ISSN (online)	1615-9861
ISSN	1615-9853
DOI	10.1002/pmic.200400963
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5386: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.A 1868: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Book ; Online: Sedimentology and gas hydrates of IODP Expedition 311 samples, supplementary data to: Torres, Marta E; Tréhu, Anne M; Cespedes, N; Kastner, Miriam; Wortmann, Ulrich G; Kim, Jung-Hyun; Long, Philip E; Malinverno, Alberto; Pohlman, John W; Riedel, Michael; Collett, Tim S (2008): Methane hydrate formation in turbidite sediments of northern Cascadia, IODP Expedition 311. Earth and Planetary Science Letters, 271(1-4), 170-180

Torres, Marta E / Cespedes, N / Kastner, Miriam / Kim, Jung-Hyun / Long, Philip E / Malinverno, Alberto / Pohlman, John W / Tréhu, Anne M / Wortmann, Ulrich G / al., et

2008

Abstract: Expedition 311 of the Integrated Ocean Drilling Program (IODP) to northern Cascadia recovered gas-hydrate bearing sediments along a SW-NE transect from the first ridge of the accretionary margin to the eastward limit of gas-hydrate stability. In this ... ...

Abstract	Expedition 311 of the Integrated Ocean Drilling Program (IODP) to northern Cascadia recovered gas-hydrate bearing sediments along a SW-NE transect from the first ridge of the accretionary margin to the eastward limit of gas-hydrate stability. In this study we contrast the gas gas-hydrate distribution from two sites drilled ~ 8 km apart in different tectonic settings. At Site U1325, drilled on a depositional basin with nearly horizontal sedimentary sequences, the gas-hydrate distribution shows a trend of increasing saturation toward the base of gas-hydrate stability, consistent with several model simulations in the literature. Site U1326 was drilled on an uplifted ridge characterized by faulting, which has likely experienced some mass wasting events. Here the gas hydrate does not show a clear depth-distribution trend, the highest gas-hydrate saturation occurs well within the gas-hydrate stability zone at the shallow depth of ~ 49 mbsf. Sediments at both sites are characterized by abundant coarse-grained (sand) layers up to 23 cm in thickness, and are interspaced within fine-grained (clay and silty clay) detrital sediments. The gas-hydrate distribution is punctuated by localized depth intervals of high gas-hydrate saturation, which preferentially occur in the coarse-grained horizons and occupy up to 60% of the pore space at Site U1325 and > 80% at Site U1326. Detailed analyses of contiguous samples of different lithologies show that when enough methane is present, about 90% of the variance in gas-hydrate saturation can be explained by the sand (> 63 æm) content of the sediments. The variability in gas-hydrate occupancy of sandy horizons at Site U1326 reflects an insufficient methane supply to the sediment section between 190 and 245 mbsf.
Language	English
Dates of publication	2008-9999
Size	Online-Ressource
Publisher	PANGAEA - Data Publisher for Earth & Environmental Science
Publishing place	Bremen/Bremerhaven
Document type	Book ; Online
Note	This dataset is supplement to doi:10.1016/j.epsl.2008.03.061
DOI	10.1594/PANGAEA.716589
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Book ; Online: Geochemistry and morphometry on planktonic foraminifera, supplementary data to: de Moel, Hans; Ganssen, Gerald M; Peeters, Frank J C; Jung, Simon J A; Kroon, Dick; Brummer, Geert-Jan A; Zeebe, Richard E (2009): Planktic foraminiferal shell thinning in the Arabian Sea due to anthropogenic ocean acidification?. Biogeosciences, 6, 1917-1925

de Moel, Hans / Brummer, Geert-Jan A / Ganssen, Gerald M / Jung, Simon J A / Kroon, Dick / Peeters, Frank J C / Zeebe, Richard E

2010

Abstract: About one third of the anthropogenic carbon dioxide (CO2) released into the atmosphere in the past two centuries has been taken up by the ocean. As CO2 invades the surface ocean, carbonate ion concentrations and pH are lowered. Laboratory studies ... ...

Abstract	About one third of the anthropogenic carbon dioxide (CO2) released into the atmosphere in the past two centuries has been taken up by the ocean. As CO2 invades the surface ocean, carbonate ion concentrations and pH are lowered. Laboratory studies indicate that this reduces the calcification rates of marine calcifying organisms, including planktic foraminifera. Such a reduction in calcification resulting from anthropogenic CO2 emissions has not been observed, or quantified in the field yet. Here we present the findings of a study in the Western Arabian Sea that uses shells of the surface water dwelling planktic foraminifer Globigerinoides ruber in order to test the hypothesis that anthropogenically induced acidification has reduced shell calcification of this species. We found that light, thin-walled shells from the surface sediment are younger (based on 14C and d13C measurements) than the heavier, thicker-walled shells. Shells in the upper, bioturbated, sediment layer were significantly lighter compared to shells found below this layer. These observations are consistent with a scenario where anthropogenically induced ocean acidification reduced the rate at which foraminifera calcify, resulting in lighter shells. On the other hand, we show that seasonal upwelling in the area also influences their calcification and the stable isotope (d13C and d18O) signatures recorded by the foraminifera shells. Plankton tow and sediment trap data show that lighter shells were produced during upwelling and heavier ones during non-upwelling periods. Seasonality alone, however, cannot explain the 14C results, or the increase in shell weight below the bioturbated sediment layer. We therefore must conclude that probably both the processes of acidification and seasonal upwelling are responsible for the presence of light shells in the top of the sediment and the age difference between thick and thin specimens.
Language	English
Dates of publication	2010-9999
Size	Online-Ressource
Publisher	PANGAEA - Data Publisher for Earth & Environmental Science
Publishing place	Bremen/Bremerhaven
Document type	Book ; Online
Note	This dataset is supplement to doi:10.5194/bg-6-1917-2009
DOI	10.1594/PANGAEA.746072
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED