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  1. AU=Cao Yongsen
  2. AU="Mei, Guoliang"
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  1. Artikel ; Online: Conferring Resistance to Plant RNA Viruses with the CRISPR/CasRx System.

    Cao, Yongsen / Zhou, Huanbin / Zhou, Xueping / Li, Fangfang

    Virologica Sinica

    2021  Band 36, Heft 4, Seite(n) 814–817

    Mesh-Begriff(e) CRISPR-Cas Systems ; Clustered Regularly Interspaced Short Palindromic Repeats ; Plant Diseases ; Plant Viruses/genetics ; RNA Viruses/genetics ; RNA, Plant
    Chemische Substanzen RNA, Plant
    Sprache Englisch
    Erscheinungsdatum 2021-01-18
    Erscheinungsland China
    Dokumenttyp Letter
    ZDB-ID 1011219-4
    ISSN 1995-820X ; 1000-3223 ; 1003-5125
    ISSN (online) 1995-820X
    ISSN 1000-3223 ; 1003-5125
    DOI 10.1007/s12250-020-00338-8
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  2. Artikel: Control of Plant Viruses by CRISPR/Cas System-Mediated Adaptive Immunity.

    Cao, Yongsen / Zhou, Huanbin / Zhou, Xueping / Li, Fangfang

    Frontiers in microbiology

    2020  Band 11, Seite(n) 593700

    Abstract: Plant diseases caused by invading plant viruses pose serious threats to agricultural production in the world, and the antiviral engineering initiated by molecular biotechnology has been an effective strategy to prevent and control plant viruses. Recent ... ...

    Abstract Plant diseases caused by invading plant viruses pose serious threats to agricultural production in the world, and the antiviral engineering initiated by molecular biotechnology has been an effective strategy to prevent and control plant viruses. Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-mediated DNA or RNA editing/interference in plants make them very attractive tools applicable to the plant protection field. Here, we review the development of CRISPR/Cas systems and summarize their applications in controlling different plant viruses by targeting viral sequences or host susceptibility genes. We list some potential recessive resistance genes that can be utilized in antiviral breeding and emphasize the importance and promise of recessive resistance gene-based antiviral breeding to generate transgene-free plants without developmental defects. Finally, we discuss the challenges and opportunities for the application of CRISPR/Cas techniques in the prevention and control of plant viruses in the field.
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-10-26
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.593700
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Tonifying-Qi-and-Detoxification Decoction attenuated injuries of colon and lung tissues in ulcerative colitis rat model via regulating NF-κB and p38MAPK pathway.

    Yan, Xin / Yu, Xue / Jiang, Chunhua / Cao, Ying / Zhu, Liang / Du, Chenguang / Jia, Yongsen

    Annals of translational medicine

    2022  Band 10, Heft 8, Seite(n) 455

    Abstract: Background: Tonifying-Qi-and-Detoxification Decoction (TQDD) is a Chinese medicine compound. This research probed the possible protective effects of TQDD on injuries of the colon and lung tissues in ulcerative colitis (UC) rat model.: Methods: UC rat ...

    Abstract Background: Tonifying-Qi-and-Detoxification Decoction (TQDD) is a Chinese medicine compound. This research probed the possible protective effects of TQDD on injuries of the colon and lung tissues in ulcerative colitis (UC) rat model.
    Methods: UC rat model was established by colon mucosal tissue sensitization combined with TNBS-ethanol. Ninety-six rats were randomly divided into normal control (NC), model, sulfasalazine (SASP), and TQDD (low, middle, and high dosages) groups. After 4 weeks intervention, all rats were sacrificed. The microstructure of lung tissue was observed using hematoxylin-eosin (HE) staining. Transmission electron microscope (TEM) was utilized to assess the ultrastructure change of alveolar epithelial type II cells (AEC-II). The mRNA expressions of Bax, Caspase 3, nuclear factor kappa B (NF-κB) and NF-κB inhibitor α (IKBα) in tissues were measured via quantitative reverse transcription PCR (qRT-PCR) assay. Western blotting and immunohistochemistry (IHC) were used to test p38MAPK, activating transcription factor 2 (ATF2), c-jun and c-fos expressions in tissues.
    Results: TQDD alleviated microstructure change of lung tissues, lung cell apoptosis and ultrastructure alterations of AEC-II in UC rat model. Moreover, TQDD suppressed activation of NF-κB pathway in colon and lung tissues. Besides, TQDD inhibited p38MAPK pathway in colon and lung tissues, as well as reduced ATF2, c-jun, and c-fos expressions in colon and lung tissues.
    Conclusions: This research confirmed the beneficial effect of TQDD on injuries of colon and lung tissues in UC rat model. TQDD attenuated injuries of lung and colon tissues in colon mucosal tissue sensitization combined with TNBS-ethanol-caused UC model via regulating NF-κB and p38MAPK pathways.
    Sprache Englisch
    Erscheinungsdatum 2022-05-05
    Erscheinungsland China
    Dokumenttyp Journal Article
    ZDB-ID 2893931-1
    ISSN 2305-5847 ; 2305-5839
    ISSN (online) 2305-5847
    ISSN 2305-5839
    DOI 10.21037/atm-22-892
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Letrozole and the Traditional Chinese Medicine, Shaofu Zhuyu Decoction, Reduce Endometriotic Disease Progression in Rats

    Ying Cao / Chunhua Jiang / Yongsen Jia / Dingjie Xu / Yanyan Yu

    Evidence-Based Complementary and Alternative Medicine, Vol

    A Potential Role for Gut Microbiota

    2020  Band 2020

    Abstract: We previously showed that the Chinese herbal medicine, Shaofu Zhuyu decoction (SFZYD), shrank the size of endometriotic lesions in rats with endometriosis. We therefore conducted the present study to investigate the effects of letrozole and SFZYD on gut ... ...

    Abstract We previously showed that the Chinese herbal medicine, Shaofu Zhuyu decoction (SFZYD), shrank the size of endometriotic lesions in rats with endometriosis. We therefore conducted the present study to investigate the effects of letrozole and SFZYD on gut microbiota in endometriotic rats. Rats were divided into four groups: a blank group, model group, letrozole group, and SFZY group. Ectopic lesion size and COX-2 expression in the endometrium and endometriotic lesions were compared, and the community of gut microbiota was detected using 16S rRNA gene sequencing. Both letrozole and SFZYD reduced the size of ectopic lesions as well as lowered the expression of COX-2, thus reducing the inflammatory response. Compared with the blank group, the α-diversity of gut microbiota in endometriotic rats decreased, the Firmicutes/Bacteroidetes ratio increased, and the abundance of Ruminococcaceae was reduced. The α-diversity of gut microbiota in the letrozole group was similar to that in the model group, but the Firmicutes/Bacteroidetes ratio was diminished. The α-diversity in the SFZY group was similar to that in the blank group, the Firmicutes/Bacteroidetes ratio was attenuated, and the abundance of Ruminococcaceae was elevated compared with the model group. These results indicated that the therapeutic mechanisms of both letrozole and SFZYD were related to the restoration of gut microbiota.
    Schlagwörter Other systems of medicine ; RZ201-999
    Sprache Englisch
    Erscheinungsdatum 2020-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
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  5. Artikel: Letrozole and the Traditional Chinese Medicine, Shaofu Zhuyu Decoction, Reduce Endometriotic Disease Progression in Rats: A Potential Role for Gut Microbiota.

    Cao, Ying / Jiang, Chunhua / Jia, Yongsen / Xu, Dingjie / Yu, Yanyan

    Evidence-based complementary and alternative medicine : eCAM

    2020  Band 2020, Seite(n) 3687498

    Abstract: We previously showed that the Chinese herbal medicine, Shaofu Zhuyu decoction (SFZYD), shrank the size of endometriotic lesions in rats with endometriosis. We therefore conducted the present study to investigate the effects of letrozole and SFZYD on gut ... ...

    Abstract We previously showed that the Chinese herbal medicine, Shaofu Zhuyu decoction (SFZYD), shrank the size of endometriotic lesions in rats with endometriosis. We therefore conducted the present study to investigate the effects of letrozole and SFZYD on gut microbiota in endometriotic rats. Rats were divided into four groups: a blank group, model group, letrozole group, and SFZY group. Ectopic lesion size and COX-2 expression in the endometrium and endometriotic lesions were compared, and the community of gut microbiota was detected using 16S rRNA gene sequencing. Both letrozole and SFZYD reduced the size of ectopic lesions as well as lowered the expression of COX-2, thus reducing the inflammatory response. Compared with the blank group, the
    Sprache Englisch
    Erscheinungsdatum 2020-07-20
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2020/3687498
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  6. Artikel: Effects of lime amendment on the organic substances changes, antibiotics removal, and heavy metals speciation transformation during swine manure composting

    Chen, Zhiqiang / Fu, Qiqi / Cao, Yongsen / Wen, Qinxue / Wu, Yiqi

    Chemosphere. 2021 Jan., v. 262

    2021  

    Abstract: Aerobic composting has been used for a long time for bioconversion of manure wastes, however, its application has been limited due to slow transition of organic matters and influence of heavy metals and antibiotics residues. Compost with lime addition ... ...

    Abstract Aerobic composting has been used for a long time for bioconversion of manure wastes, however, its application has been limited due to slow transition of organic matters and influence of heavy metals and antibiotics residues. Compost with lime addition can speed up the composting process, while its effects on the evolution of organic matters, heavy metals and antibiotics need to be further investigated. In this research, the effects of lime amendment on organic substances changes was assessed by the spectroscopic characteristics. Besides, chlortetracycline (CTC) removal and Cu, Zn chemical speciation transformation were also evaluated. Results showed that the humic acid-like substances region of fluorescence regional integration (FRI-EEM) increased from 20.5% to 40.9% and 20.6%–32.6%, respectively, in lime addition treatment and control after 15 days of composting, indicating that the addition of lime remarkably improved the transition of organic matter and accelerated the maturity process. Besides, 94.04% of CTC in the manure was removed when lime was added, higher than 86.10% in the control group. The transformation of zinc from exchangeable and reducible into oxidizable and residual fractions was improved while the transformation of copper was affected slightly. Therefore, lime is a suitable amendment material for manure composting, which can accelerate the transition of organic matters due to the regulation of composting pH, as well as eliminate harmful CTC and bioavailable heavy metal, thus promoting the further utilizing of organic substance.
    Schlagwörter bioavailability ; biotransformation ; chemical speciation ; chlortetracycline ; composts ; copper ; evolution ; fluorescence ; heavy metals ; organic matter ; pH ; pig manure ; spectroscopy ; zinc
    Sprache Englisch
    Erscheinungsverlauf 2021-01
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    Anmerkung NAL-light
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2020.128342
    Datenquelle NAL Katalog (AGRICOLA)

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  7. Artikel ; Online: Effects of lime amendment on the organic substances changes, antibiotics removal, and heavy metals speciation transformation during swine manure composting.

    Chen, Zhiqiang / Fu, Qiqi / Cao, Yongsen / Wen, Qinxue / Wu, Yiqi

    Chemosphere

    2020  Band 262, Seite(n) 128342

    Abstract: Aerobic composting has been used for a long time for bioconversion of manure wastes, however, its application has been limited due to slow transition of organic matters and influence of heavy metals and antibiotics residues. Compost with lime addition ... ...

    Abstract Aerobic composting has been used for a long time for bioconversion of manure wastes, however, its application has been limited due to slow transition of organic matters and influence of heavy metals and antibiotics residues. Compost with lime addition can speed up the composting process, while its effects on the evolution of organic matters, heavy metals and antibiotics need to be further investigated. In this research, the effects of lime amendment on organic substances changes was assessed by the spectroscopic characteristics. Besides, chlortetracycline (CTC) removal and Cu, Zn chemical speciation transformation were also evaluated. Results showed that the humic acid-like substances region of fluorescence regional integration (FRI-EEM) increased from 20.5% to 40.9% and 20.6%-32.6%, respectively, in lime addition treatment and control after 15 days of composting, indicating that the addition of lime remarkably improved the transition of organic matter and accelerated the maturity process. Besides, 94.04% of CTC in the manure was removed when lime was added, higher than 86.10% in the control group. The transformation of zinc from exchangeable and reducible into oxidizable and residual fractions was improved while the transformation of copper was affected slightly. Therefore, lime is a suitable amendment material for manure composting, which can accelerate the transition of organic matters due to the regulation of composting pH, as well as eliminate harmful CTC and bioavailable heavy metal, thus promoting the further utilizing of organic substance.
    Mesh-Begriff(e) Aerobiosis ; Animals ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/metabolism ; Biodegradation, Environmental ; Calcium Compounds ; Chlortetracycline/chemistry ; Chlortetracycline/metabolism ; Composting/methods ; Copper/chemistry ; Humic Substances ; Manure ; Metals, Heavy/chemistry ; Oxides ; Soil/chemistry ; Spectroscopy, Fourier Transform Infrared ; Swine ; Temperature ; Zinc/chemistry
    Chemische Substanzen Anti-Bacterial Agents ; Calcium Compounds ; Humic Substances ; Manure ; Metals, Heavy ; Oxides ; Soil ; Copper (789U1901C5) ; lime (C7X2M0VVNH) ; Zinc (J41CSQ7QDS) ; Chlortetracycline (WCK1KIQ23Q)
    Sprache Englisch
    Erscheinungsdatum 2020-09-14
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2020.128342
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel: Analysis of the Anti-Tumour Effect of Xuefu Zhuyu Decoction Based on Network Pharmacology and Experimental Verification in

    Wang, Sitong / Wu, Chenxi / Li, Yinghong / Ye, Bin / Wang, Shuai / Li, Guowang / Wu, Jiawei / Liu, Shengnan / Zhang, Menglong / Jia, Yongsen / Cao, Huijuan / Jiang, Chunhua / Wu, Fanwu

    Frontiers in pharmacology

    2022  Band 13, Seite(n) 922457

    Abstract: Background: ...

    Abstract Background:
    Sprache Englisch
    Erscheinungsdatum 2022-07-12
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2022.922457
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel: Anticancer Activity of

    Jia, Yongsen / Yan, Xin / Cao, Ying / Song, Wei / Zhang, Guangji / Hu, Xueqin

    Evidence-based complementary and alternative medicine : eCAM

    2020  Band 2020, Seite(n) 3053506

    Abstract: Background: To explore the activity of : Methods: Herbal compounds were prepared by ethanol extraction, and 6 herbs composing into MTD were dipped in water-free ethanol and filtered. The filtrate was collected and centrifuged. The remains were ... ...

    Abstract Background: To explore the activity of
    Methods: Herbal compounds were prepared by ethanol extraction, and 6 herbs composing into MTD were dipped in water-free ethanol and filtered. The filtrate was collected and centrifuged. The remains were concentrated into a paste which was adjusted to 5000mg/mL concentration with DMSO. PBS was used to dilute the herbal solution to the half maximal inhibitory concentration. A hypoxic microenvironment was induced with CoCl
    Results: The fifty percent inhibitory dose of MTD was 1410 
    Conclusion: MTD downregulated HIF-1
    Sprache Englisch
    Erscheinungsdatum 2020-09-29
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2020/3053506
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Anticancer Activity of Modified Tongyou Decoction on Eca109 Esophageal Cancer Cell Invasion and Metastasis through Regulation of the Epithelial-Mesenchymal Transition Mediated by the HIF-1α-Snail Axis

    Yongsen Jia / Xin Yan / Ying Cao / Wei Song / Guangji Zhang / Xueqin Hu

    Evidence-Based Complementary and Alternative Medicine, Vol

    2020  Band 2020

    Abstract: Background. To explore the activity of Modified Tongyou Decoction (MTD) against Eca109 esophageal cancer (EC) cell invasion and metastasis and to ascertain the mechanism of its anticancer activity during the epithelial-mesenchymal transition (EMT) as ... ...

    Abstract Background. To explore the activity of Modified Tongyou Decoction (MTD) against Eca109 esophageal cancer (EC) cell invasion and metastasis and to ascertain the mechanism of its anticancer activity during the epithelial-mesenchymal transition (EMT) as mediated by the HIF-1α-Snail axis. Methods. Herbal compounds were prepared by ethanol extraction, and 6 herbs composing into MTD were dipped in water-free ethanol and filtered. The filtrate was collected and centrifuged. The remains were concentrated into a paste which was adjusted to 5000mg/mL concentration with DMSO. PBS was used to dilute the herbal solution to the half maximal inhibitory concentration. A hypoxic microenvironment was induced with CoCl2 in RPMI 1640 medium, in which Eca109 cells were cultured. The cytotoxicity of MTD was determined with CCK-8 assay. The activity of MTD against cell invasion and metastasis was explored with scratch assay and transwell assay. Western blot analysis was conducted to analyze the anticancer effects of MTD on the expression of HIF-1α-Snail axis- and EMT-related proteins. Quantitative RT-PCR was used to assess the mRNA expression of Snail. Immunofluorescence labeling was performed to examine how MTD affected the coexpression of Snail and HIF-1α. Results. The fifty percent inhibitory dose of MTD was 1410 μg/mL in the normoxic environment and 1823 μg/mL in the hypoxic environment based on the CCK-8 assay. The scratch assay showed that MTD significantly inhibited cell migration in both the normoxic and hypoxic microenvironments compared with the control groups (P < 0.05). The transwell assay showed that MTD significantly inhibited cell invasion in both the normoxic and hypoxic environments compared with the control groups (P < 0.05). Western blot showed that MTD significantly inhibited the expression of the HIF-1α, Snail, Vimentin, MMP-2, MMP-9, and VE-cadherin proteins and significantly induced the expression of E-cadherin in both the normoxic and hypoxic microenvironments compared with the control groups (P < ...
    Schlagwörter Other systems of medicine ; RZ201-999
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2020-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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