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  1. Article ; Online: Preclinical models of breast cancer: B6BC, a transplantable hormone receptor-positive C57BL/6 mouse cell line.

    Perez-Lanzon, Maria / Maiuri, Maria Chiara / Lopez-Otin, Carlos / Kroemer, Guido

    Genes and immunity

    2024  Volume 25, Issue 2, Page(s) 172–174

    MeSH term(s) Mice ; Animals ; Mice, Inbred C57BL ; Cell Line, Tumor ; Neoplasms
    Language English
    Publishing date 2024-01-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2060566-3
    ISSN 1476-5470 ; 1466-4879
    ISSN (online) 1476-5470
    ISSN 1466-4879
    DOI 10.1038/s41435-023-00241-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5592: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Correction: Preclinical models of breast cancer: B6BC, a transplantable hormone receptor-positive C57BL/6 mouse cell line.

    Perez-Lanzon, Maria / Maiuri, Maria Chiara / Lopez-Otin, Carlos / Kroemer, Guido

    Genes and immunity

    2024  Volume 25, Issue 2, Page(s) 175

    Language English
    Publishing date 2024-02-20
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2060566-3
    ISSN 1476-5470 ; 1466-4879
    ISSN (online) 1476-5470
    ISSN 1466-4879
    DOI 10.1038/s41435-024-00259-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5592: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Sub-50 fs Formation of Charge Transfer States Rules the Fate of Photoexcitations in Eumelanin-Like Materials.

    Petropoulos, Vasilis / Mavridi-Printezi, Alexandra / Menichetti, Arianna / Mordini, Dario / Kabacinski, Piotr / Gianneschi, Nathan C / Montalti, Marco / Maiuri, Margherita / Cerullo, Giulio

    The journal of physical chemistry letters

    2024  Volume 15, Issue 13, Page(s) 3639–3645

    Abstract: Eumelanins play a crucial role as photoprotective agents for living organisms, yet the nature of the stationary and transient species involved in the light absorption and deactivation processes remains controversial. Moreover, the critical sub-100 fs ... ...

    Abstract Eumelanins play a crucial role as photoprotective agents for living organisms, yet the nature of the stationary and transient species involved in the light absorption and deactivation processes remains controversial. Moreover, the critical sub-100 fs time scale, which is key to the characterization of the primary excited species, has remained unexplored. Here, we study the eumelanin analogue polydopamine (PDA) and employ a combination of steady-state and transient optical spectroscopies to reveal the presence of spectrally broad coupled electronic transitions with, at least partial, charge-transfer (CT) character. We monitor the CT state dynamics using tunable sub-20 fs pulses. We find that high photon energy excitation results in accelerated (sub-20 fs) CT formation times while activating pathways, which lead to long-lived (≫1 ns), possibly reactive CT species. On the other hand, visible light excitation results in a slower (≈45 fs) formation of bound CT states, which, however, recombine on the ultrafast sub-2 ps time scale.
    Language English
    Publishing date 2024-03-26
    Publishing country United States
    Document type Journal Article
    ISSN 1948-7185
    ISSN (online) 1948-7185
    DOI 10.1021/acs.jpclett.4c00170
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Microscopic investigation of intrinsic defects in CVD grown MoS

    Senkić, Ana / Supina, Antonio / Akturk, Mert / Gadermaier, Christoph / Maiuri, Margherita / Cerullo, Giulio / Vujičić, Nataša

    Nanotechnology

    2023  Volume 34, Issue 47

    Abstract: Defects in two-dimensional (2D) transition metal dichalcogenides (TMDs) greatly influence their electronic and optical properties by introducing localized in-gap states. Using different non-invasive techniques, we have investigated the spatial ... ...

    Abstract Defects in two-dimensional (2D) transition metal dichalcogenides (TMDs) greatly influence their electronic and optical properties by introducing localized in-gap states. Using different non-invasive techniques, we have investigated the spatial distribution of intrinsic defects in as-grown chemical vapor deposition (CVD) MoS
    Language English
    Publishing date 2023-09-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 1362365-5
    ISSN 1361-6528 ; 0957-4484
    ISSN (online) 1361-6528
    ISSN 0957-4484
    DOI 10.1088/1361-6528/acf29b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Isolation of Primary Mouse Hepatocytes and Non-Parenchymal Cells from a Liver with Precancerous Lesions.

    Lambertucci, Flavia / Motiño, Omar / Pérez-Lanzón, Maria / Li, Sijing / Plantureux, Céleste / Pol, Jonathan / Maiuri, Maria Chiara / Kroemer, Guido / Martins, Isabelle

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2769, Page(s) 109–128

    Abstract: In the early stages of liver carcinogenesis, rare hepatocytes and cholangiocytes are transformed into preneoplastic cells, which can progressively acquire a neoplastic phenotype, favored by the failure of natural antitumor immunosurveillance. The ... ...

    Abstract In the early stages of liver carcinogenesis, rare hepatocytes and cholangiocytes are transformed into preneoplastic cells, which can progressively acquire a neoplastic phenotype, favored by the failure of natural antitumor immunosurveillance. The detailed study of both hepatic parenchymal (e.g., hepatocytes) and non-parenchymal cells (NPCs), such as immune cells, could help understand the cellular microenvironment surrounding these pre-cancerous and neoplastic lesions.Cultures of primary hepatocytes are of interest in various biomedical research disciplines, serving as an ex vivo model for liver physiology. Obtaining high viability and yield of primary mouse hepatocytes and other liver cell populations is technically challenging, thus limiting their use. In the first section of the current chapter, we introduce a protocol based on the two-step collagenase perfusion technique (by inferior vena cava) to isolate hepatocytes and, to a lower extent, NPCs and detailed the different considerations to take into account for a successful perfusion. The liver is washed by perfusion, hepatocytes are dissociated with collagenase, and different cell populations are separated by centrifugation. Various techniques have been described for the isolation of healthy and malignant hepatocytes; however, the viability and purity of the isolated cells is frequently not satisfactory. Here, we significantly optimized this protocol to reach improved yield and viability of the hepatocytes and concomitantly obtain preserved NPC populations of the liver.Within NPCs, tissue-resident or recruited immune cells are essential actors regulating hepatocarcinogenesis. However, simultaneous isolation of hepatic leukocytes together with other cell types generally yields low immune cell numbers hindering downstream application with these cells. In the second section of this chapter, as opposed to the first section primarily aiming to isolate hepatocytes, we present a tissue dissociation protocol adapted to efficiently recover leukocytes from non-perfused bulk (pre-)cancerous livers. This protocol has been optimized to be operator-friendly and fast compared to other liver processing methods, allowing easy simultaneous sample processing to retrieve hepatic (tumor-infiltrating) immune cells.
    MeSH term(s) Mice ; Animals ; Cell Separation/methods ; Liver ; Hepatocytes ; Precancerous Conditions ; Carcinogenesis ; Collagenases ; Tumor Microenvironment
    Chemical Substances Collagenases (EC 3.4.24.-)
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3694-7_9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Oncogene-Driven Induction of Orthotopic Cholangiocarcinoma in Mice.

    Plantureux, Céleste / Paillet, Juliette / Autret, Gwennhael / Pérez-Lanzón, Maria / Kroemer, Guido / Maiuri, Maria Chiara / Pol, Jonathan

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2769, Page(s) 99–108

    Abstract: Cholangiocarcinoma (CCA) is a malignancy affecting the epithelial cells that line the bile ducts. This cancer shows a poor prognosis and current treatments remain inefficient. Orthotopic CCA mouse models are useful for the development of innovative ... ...

    Abstract Cholangiocarcinoma (CCA) is a malignancy affecting the epithelial cells that line the bile ducts. This cancer shows a poor prognosis and current treatments remain inefficient. Orthotopic CCA mouse models are useful for the development of innovative therapeutic strategies. Here, we describe an orthotopic model of intrahepatic CCA that can be easily induced in mice within 5 weeks at a high incidence. It is achieved by expressing two oncogenes, namely, (i) the intracellular domain of the Notch1 receptor (NICD) and (ii) AKT, in hepatocytes by means of the sleeping beauty transposon system. These plasmid vectors are delivered by hydrodynamic injection into the tail vein. The present chapter also describes how to perform magnetic resonance imaging (MRI) of the livers to visualize intrahepatic CCA nodules.
    MeSH term(s) Mice ; Animals ; Bile Ducts, Intrahepatic ; Bile Duct Neoplasms/genetics ; Cholangiocarcinoma/genetics ; Oncogenes/genetics ; Liver/pathology
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3694-7_8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Flow Cytometry Assessment of Lymphocyte Populations Infiltrating Liver Tumors.

    Pérez-Lanzón, Maria / Plantureux, Céleste / Paillet, Juliette / Sotty, Jules / Soussan, Patrick / Kroemer, Guido / Maiuri, Maria Chiara / Pol, Jonathan

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2769, Page(s) 129–141

    Abstract: Tissue-resident and recruited immune cells are essential mediators of natural and therapy-induced immunosurveillance of liver neoplasia. This idea has been recently reinforced by the clinical approval of immune checkpoint inhibitors for the immunotherapy ...

    Abstract Tissue-resident and recruited immune cells are essential mediators of natural and therapy-induced immunosurveillance of liver neoplasia. This idea has been recently reinforced by the clinical approval of immune checkpoint inhibitors for the immunotherapy of hepatocellular carcinoma and cholangiocarcinoma. Such research progress relies on the in-depth characterization of the immune populations that are present in pre-neoplastic and neoplastic hepatic lesions. A convenient technology for advancing along this path is high-dimensional cytometry.In this chapter, we present a protocol to assess the subtype and differentiation state of hepatic lymphocyte populations by multicolor immunofluorescence staining and flow cytometry. We detail the steps required for viability assessment and immune cell phenotyping of single-cell suspensions of liver cells by means of surface and intracellular staining of more than a dozen markers of interest. This protocol does not require prior removal of debris and dead cells and allows to process multiple samples in parallel. The procedure includes the use of a fixative-resistant viability dye that allows cell fixation and permeabilization after cell surface staining and before intracellular staining and data acquisition on a flow cytometer. Moreover, we provide a panel of fluorochrome-labeled antibodies designed for the characterization of lymphocytic subsets that can be adapted to distinct experimental settings. Finally, we present an overview of the post-staining pipeline, including data acquisition on a flow cytometer and tools for post-acquisition analyses.
    MeSH term(s) Humans ; Flow Cytometry/methods ; Lymphocyte Subsets ; Liver Neoplasms ; Bile Duct Neoplasms ; Bile Ducts, Intrahepatic
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Review ; Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3694-7_10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Mammary carcinoma: toward a realistic mouse model of incurable cancers.

    Pérez-Lanzón, Maria / Maiuri, Maria Chiara / Lopez-Otin, Carlos / Kroemer, Guido

    Oncoimmunology

    2023  Volume 12, Issue 1, Page(s) 2240613

    Abstract: As long as breast cancer (BC) stays under immunosurveillance, it can be controlled by treatments eliciting anticancer immune responses. However, once BC escapes immunosurveillance, it becomes therapeutically uncontrollable. A paper in ... ...

    Abstract As long as breast cancer (BC) stays under immunosurveillance, it can be controlled by treatments eliciting anticancer immune responses. However, once BC escapes immunosurveillance, it becomes therapeutically uncontrollable. A paper in the
    MeSH term(s) Mice ; Animals ; Mice, Inbred C57BL ; Immunotherapy ; Carcinoma
    Language English
    Publishing date 2023-08-03
    Publishing country United States
    Document type Editorial ; Research Support, Non-U.S. Gov't
    ZDB-ID 2645309-5
    ISSN 2162-402X ; 2162-402X
    ISSN (online) 2162-402X
    ISSN 2162-402X
    DOI 10.1080/2162402X.2023.2240613
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Editorial: Vibrationally-Mediated Chemical Dynamics.

    Dean, Jacob C / Bennett, Doran I G / Staniforth, Michael / Maiuri, Margherita

    Frontiers in chemistry

    2021  Volume 9, Page(s) 681457

    Language English
    Publishing date 2021-04-20
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2711776-5
    ISSN 2296-2646
    ISSN 2296-2646
    DOI 10.3389/fchem.2021.681457
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  10. Article ; Online: Ultrafast Photophysics of Regioisomeric Triphenylamine Dyes Having Dipolar D-π-A-A and Octupolar D-(π-A-A)

    Petropoulos, Vasilis / Georgoulis, Ioannis / Vourdaki, Chrisovalantou / Hrobárik, Peter / Sigmundová, Ivica / Nociarová, Jela / Maiuri, Margherita / Cerullo, Giulio / Fakis, Mihalis

    Chemphyschem : a European journal of chemical physics and physical chemistry

    2023  Volume 24, Issue 13, Page(s) e202300127

    Abstract: Benzothiazole is among prominent electron-withdrawing heteroarene moieties used in a variety of π-conjugated molecules. Its relative orientation with respect to the principal dipole vector(s) of chromophores derived thereof is crucial, affecting ... ...

    Abstract Benzothiazole is among prominent electron-withdrawing heteroarene moieties used in a variety of π-conjugated molecules. Its relative orientation with respect to the principal dipole vector(s) of chromophores derived thereof is crucial, affecting photophysical and nonlinear optical properties. Here we compare the photophysics and ultrafast dynamics of dipolar and octupolar molecules comprising a triphenylamine electron-donating core, ethynylene π-conjugated linker(s) and benzothiazole acceptor(s) having the matched or mismatched orientation (with respect to the direction of intramolecular charge transfer), while a carbaldehyde group is attached as an auxiliary acceptor. Among chromophores without the auxiliary acceptor, stronger fluorescence solvatochromism and faster excited state dynamics are exhibited for the derivatives with the mismatched geometry. On the contrary, introduction of the auxiliary acceptor to the benzothiazole unit enhances the intramolecular charge transfer ICT (featuring ultrafast dynamics of the excited state) for the matched geometry. The data confirm the crucial role of the relative orientation of asymmetric heteroaromatic unit (regioisomeric effect) in dipolar as well as in multipolar molecules in tuning linear and nonlinear optical properties as well as excited state dynamics.
    Language English
    Publishing date 2023-05-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2025223-7
    ISSN 1439-7641 ; 1439-4235
    ISSN (online) 1439-7641
    ISSN 1439-4235
    DOI 10.1002/cphc.202300127
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