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Article ; Online: Factors associated with extent of COVID-19 outbreaks: A prospective study in a large hospital network.

Duverger, Clarisse / Monteil, Catherine / Souyri, Valérie / Fournier, Sandra

2024

Abstract: Background: The COVID-19 pandemic has generated numerous hospital outbreaks. This study aimed to identify factors related to the extent of nosocomial COVID-19 outbreaks in the largest French public health institution.: Methods: An observational study ...

Abstract	Background: The COVID-19 pandemic has generated numerous hospital outbreaks. This study aimed to identify factors related to the extent of nosocomial COVID-19 outbreaks in the largest French public health institution. Methods: An observational study was conducted from July 2020 to September 2021. Outbreaks were defined as at least 2 cases, patients and/or health care workers (HCWs), linked by time and geographic location. Logistic regression was performed to identify risk factors for large outbreaks among nine variables: variant, medical ward, COVID-19 vaccination rate and incidence among HCWs and Paris population, number of weekly COVID-19 tests among HCWs and the positivity rate, epidemic waves. Results: Within 14 months, 799 outbreaks were identified: 450 small ones (≤6 cases) and 349 large ones (≥7 cases), involving 3,260 patients and 3,850 HCWs. In univariate analysis, large outbreaks were positively correlated to geriatrics wards, COVID-19 incidence, and rate of weekly positive tests among HCWs; and negatively correlated to intensive care units, variant Delta, fourth wave, vaccination rates of the Paris region's population and that of the HCWs. In multivariate analysis, factors that remained significant were the type of medical ward and the vaccination rate among HCWs. Conclusions: Intensive care unit and high vaccination rates among HCWs were associated with a lower risk of large COVID-19 outbreaks, as opposed to geriatric wards, which are associated with a higher risk.
Language	English
Publishing date	2024-01-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	392362-9
ISSN	1527-3296 ; 0196-6553
ISSN (online)	1527-3296
ISSN	0196-6553
DOI	10.1016/j.ajic.2024.01.004
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Article ; Online: A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community.

Vallin, Elodie / Gallagher, Joseph / Granger, Laure / Martin, Edwige / Belougne, Jérôme / Maurizio, Julien / Duverger, Yohann / Scaglione, Sarah / Borrel, Caroline / Cortier, Elisabeth / Abouzid, Karima / Carre-Pierrat, Maité / Gieseler, Kathrin / Ségalat, Laurent / Kuwabara, Patricia E / Ewbank, Jonathan J

PloS one

2012 Volume 7, Issue 2, Page(s) e30482

Abstract: Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains ... insertion alleles would therefore be of general interest to the C. elegans research community. We describe ... for the engineered deletion of essentially all C. elegans genes and the modification of more than 40 ...

Abstract	Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains carrying specific insertions of the heterologous transposon Mos1. A large collection of known Mos1 insertion alleles would therefore be of general interest to the C. elegans research community. We describe here the optimization of a semi-automated methodology for the construction of a substantial collection of Mos1 insertion mutant strains. At peak production, more than 5,000 strains were generated per month. These strains were then subject to molecular analysis, and more than 13,300 Mos1 insertions characterized. In addition to targeting directly more than 4,700 genes, these alleles represent the potential starting point for the engineered deletion of essentially all C. elegans genes and the modification of more than 40% of them. This collection of mutants, generated under the auspices of the European NEMAGENETAG consortium, is publicly available and represents an important research resource.
MeSH term(s)	Animals ; Animals, Genetically Modified ; Caenorhabditis elegans/genetics ; DNA Transposable Elements ; DNA-Binding Proteins ; Genetic Engineering/methods ; Genome/genetics ; Homologous Recombination ; Mutagenesis, Insertional ; Recombination, Genetic ; Research ; Transposases
Chemical Substances	DNA Transposable Elements ; DNA-Binding Proteins ; mariner transposases ; Transposases (EC 2.7.7.-)
Language	English
Publishing date	2012-02-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0030482
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Article: Effect of mutations of N- and C-terminal charged residues on the activity of LCAT.

Peelman, F / Vanloo, B / Verschelde, J L / Labeur, C / Caster, H / Taveirne, J / Verhee, A / Duverger, N / Vandekerckhove, J / Tavernier, J / Rosseneu, M

Journal of lipid research

2001 Volume 42, Issue 4, Page(s) 471–479

Abstract: ... conservation in the N-terminal region of LCAT, we investigated the contribution of the N- and C-terminal ... conserved among LCAT proteins from different species, were mutated in the N-terminal (residues 1;-210) and C ... that mutations of N-terminal conserved basic residues affect LCAT activity more than those in the C-terminal ...

Abstract	On the basis of structural homology calculations, we previously showed that lecithin:cholesterol acyltransferase (LCAT), like lipases, belongs to the alpha/beta hydrolase fold family. As there is higher sequence conservation in the N-terminal region of LCAT, we investigated the contribution of the N- and C-terminal conserved basic residues to the catalytic activity of this enzyme. Most basic, and some acidic residues, conserved among LCAT proteins from different species, were mutated in the N-terminal (residues 1;-210) and C-terminal (residues 211;-416) regions of LCAT. Measurements of LCAT-specific activity on a monomeric substrate, on low density lipoprotein (LDL), and on reconstituted high density lipoprotein (rHDL) showed that mutations of N-terminal conserved basic residues affect LCAT activity more than those in the C-terminal region. This agrees with the highest conservation of the alpha/beta hydrolase fold and structural homology with pancreatic lipase observed for the N-terminal region, and with the location of most of the natural mutants reported for human LCAT. The structural homology between LCAT and pancreatic lipase further suggests that residues R80, R147, and D145 of LCAT might correspond to residues R37, K107, and D105 of pancreatic lipase, which form the salt bridges D105-K107 and D105-R37. Natural and engineered mutations at residues R80, D145, and R147 of LCAT are accompanied by a substantial decrease or loss of activity, suggesting that salt bridges between these residues might contribute to the structural stability of the enzyme.
MeSH term(s)	Amino Acid Sequence ; Animals ; COS Cells ; Catalysis ; Humans ; Lipase/genetics ; Lipase/metabolism ; Models, Molecular ; Molecular Sequence Data ; Phosphatidylcholine-Sterol O-Acyltransferase/chemistry ; Phosphatidylcholine-Sterol O-Acyltransferase/genetics ; Phosphatidylcholine-Sterol O-Acyltransferase/metabolism ; Point Mutation ; Protein Conformation ; Protein Structure, Tertiary ; Sequence Alignment
Chemical Substances	Phosphatidylcholine-Sterol O-Acyltransferase (EC 2.3.1.43) ; Lipase (EC 3.1.1.3)
Language	English
Publishing date	2001-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	80154-9
ISSN	1539-7262 ; 0022-2275
ISSN (online)	1539-7262
ISSN	0022-2275
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Article: Vaccine-induced early control of hepatitis C virus infection in chimpanzees fails to impact on hepatic PD-1 and chronicity.

Rollier, Christine S / Paranhos-Baccala, Glaucia / Verschoor, Ernst J / Verstrepen, Babs E / Drexhage, Joost A R / Fagrouch, Zahra / Berland, Jean-Luc / Komurian-Pradel, Florence / Duverger, Blandine / Himoudi, Nourredine / Staib, Caroline / Meyr, Marcus / Whelan, Mike / Whelan, Joseph A / Adams, Victoria C / Adams, Victoria A / Larrea, Esther / Riezu, José I / Lasarte, Juan J /

Lasarte, Juan José / Bartosch, Birke / Cosset, Francois-L / Spaan, Willy J M / Diepolder, Helmut M / Pape, Gerd R / Sutter, Gerd / Inchauspe, Genevieve / Heeney, Jonathan L

Hepatology (Baltimore, Md.)

2007 Volume 45, Issue 3, Page(s) 602–613

Abstract: Unlabelled: Broad T cell and B cell responses to multiple HCV antigens are observed early in individuals who control or clear HCV infection. The prevailing hypothesis has been that similar immune responses induced by prophylactic immunization would ... ...

Abstract	Unlabelled: Broad T cell and B cell responses to multiple HCV antigens are observed early in individuals who control or clear HCV infection. The prevailing hypothesis has been that similar immune responses induced by prophylactic immunization would reduce acute virus replication and protect exposed individuals from chronic infection. Here, we demonstrate that immunization of naïve chimpanzees with a multicomponent HCV vaccine induced robust HCV-specific immune responses, and that all vaccinees exposed to heterologous chimpanzee-adapted HCV 1b J4 significantly reduced viral RNA in serum by 84%, and in liver by 99% as compared to controls (P=0.024 and 0.028, respectively). However, despite control of HCV in plasma and liver in the acute period, in the chronic phase, 3 of 4 vaccinated animals developed persistent infection. Analysis of expression levels of proinflammatory cytokines in serial hepatic biopsies failed to reveal an association with vaccine outcome. However, expression of IDO, CTLA-4 [corrected] and PD-1 levels in liver correlated with clearance or chronicity. Conclusion: Despite early control of virus load, a virus-associated tolerogenic-like state can develop in certain individuals independent of vaccination history.
MeSH term(s)	Animals ; Antigens, CD/metabolism ; Antigens, Viral/immunology ; Apoptosis Regulatory Proteins/metabolism ; B-Lymphocytes/immunology ; B-Lymphocytes/metabolism ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/metabolism ; Chronic Disease/prevention & control ; Cytokines/metabolism ; DNA, Viral/genetics ; Hepacivirus/genetics ; Hepacivirus/immunology ; Hepatitis C/immunology ; Hepatitis C/prevention & control ; Pan troglodytes ; Programmed Cell Death 1 Receptor ; Viral Hepatitis Vaccines/therapeutic use ; Viral Load
Chemical Substances	Antigens, CD ; Antigens, Viral ; Apoptosis Regulatory Proteins ; Cytokines ; DNA, Viral ; PDCD1 protein, human ; Programmed Cell Death 1 Receptor ; Viral Hepatitis Vaccines
Language	English
Publishing date	2007-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604603-4
ISSN	1527-3350 ; 0270-9139
ISSN (online)	1527-3350
ISSN	0270-9139
DOI	10.1002/hep.21573
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Article: Presentation of four patients operated on corneal transplants.

DUVERGER, C

Gazette medicale limousine

2014 Volume 42, Issue 9, Page(s) 433

Title translation	Présentation de quatre malades opérés de greffes de la cornée.
MeSH term(s)	Cornea
Language	French
Publishing date	2014-01-04
Publishing country	France
Document type	Journal Article
ISSN	0980-4463
ISSN	0980-4463
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Article ; Online: Decrease of carbapenemase-producing Enterobacteriaceae incidence during the first year of the COVID-19 pandemic.

Duverger, Clarisse / Monteil, Catherine / Souyri, Valérie / Fournier, Sandra

The Journal of infection

2022 Volume 85, Issue 1, Page(s) 90–122

MeSH term(s)	Anti-Bacterial Agents ; Bacterial Proteins/genetics ; COVID-19 ; Carbapenem-Resistant Enterobacteriaceae ; Enterobacteriaceae ; Enterobacteriaceae Infections/epidemiology ; Humans ; Incidence ; Microbial Sensitivity Tests ; Pandemics/prevention & control ; beta-Lactamases
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2022-04-01
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	424417-5
ISSN	1532-2742 ; 0163-4453
ISSN (online)	1532-2742
ISSN	0163-4453
DOI	10.1016/j.jinf.2022.03.024
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Article ; Online: Controlling healthcare-associated transmission of SARS-CoV-2 variant of concern 202012/01 in a large hospital network.

Duverger, C / Souyri, V / Monteil, C / Fournier, S

The Journal of hospital infection

2021 Volume 114, Page(s) 182–184

MeSH term(s)	COVID-19 ; Delivery of Health Care ; Hospitals ; Humans ; SARS-CoV-2
Language	English
Publishing date	2021-05-07
Publishing country	England
Document type	Letter
ZDB-ID	779366-2
ISSN	1532-2939 ; 0195-6701
ISSN (online)	1532-2939
ISSN	0195-6701
DOI	10.1016/j.jhin.2021.04.031
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Article: Memory T-cell-mediated immune responses specific to an alternative core protein in hepatitis C virus infection.

Bain, Christine / Parroche, Peggy / Lavergne, Jean Pierre / Duverger, Blandine / Vieux, Claude / Dubois, Valérie / Komurian-Pradel, Florence / Trépo, Christian / Gebuhrer, Lucette / Paranhos-Baccala, Glaucia / Penin, François / Inchauspé, Geneviève

Journal of virology

2004 Volume 78, Issue 19, Page(s) 10460–10469

Abstract: ... of the hepatitis C virus (HCV) core protein that was named F protein or ARFP (alternative reading frame protein) and ...

Abstract	In vitro studies have described the synthesis of an alternative reading frame form of the hepatitis C virus (HCV) core protein that was named F protein or ARFP (alternative reading frame protein) and includes a domain coded by the +1 open reading frame of the RNA core coding region. The expression of this protein in HCV-infected patients remains controversial. We have analyzed peripheral blood from 47 chronically or previously HCV-infected patients for the presence of T lymphocytes and antibodies specific to the ARFP. Anti-ARFP antibodies were detected in 41.6% of the patients infected with various HCV genotypes. Using a specific ARFP 99-amino-acid polypeptide as well as four ARFP predicted class I-restricted 9-mer peptides, we show that 20% of the patients display specific lymphocytes capable of producing gamma interferon, interleukin-10, or both cytokines. Patients harboring three different viral genotypes (1a, 1b, and 3) carried T lymphocytes reactive to genotype 1b-derived peptides. In longitudinal analysis of patients receiving therapy, both core and ARFP-specific T-cell- and B-cell-mediated responses were documented. The magnitude and kinetics of the HCV antigen-specific responses differed and were not linked with viremia or therapy outcome. These observations provide strong and new arguments in favor of the synthesis, during natural HCV infection, of an ARFP derived from the core sequence. Moreover, the present data provide the first demonstration of the presence of T-cell-mediated immune responses directed to this novel HCV antigen.
MeSH term(s)	Adult ; Aged ; Amino Acid Sequence ; Antigens, Viral/biosynthesis ; Antigens, Viral/immunology ; Antiviral Agents/therapeutic use ; B-Lymphocytes/immunology ; Hepacivirus/drug effects ; Hepacivirus/genetics ; Hepacivirus/immunology ; Hepatitis C/drug therapy ; Hepatitis C/immunology ; Hepatitis C/virology ; Hepatitis C Antibodies/blood ; Humans ; Immunologic Memory ; Interferon-alpha/therapeutic use ; Interferon-gamma/biosynthesis ; Interleukin-10/biosynthesis ; Middle Aged ; Molecular Sequence Data ; Ribavirin/therapeutic use ; Sequence Homology ; T-Lymphocytes/immunology ; Treatment Outcome ; Viral Core Proteins/biosynthesis ; Viral Core Proteins/genetics ; Viral Core Proteins/immunology ; Viremia
Chemical Substances	Antigens, Viral ; Antiviral Agents ; Hepatitis C Antibodies ; Interferon-alpha ; Viral Core Proteins ; hepatitis C protein F, Hepatitis C virus ; Interleukin-10 (130068-27-8) ; Ribavirin (49717AWG6K) ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2004-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.78.19.10460-10469.2004
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Article ; Online: Protective factors of suicidal behaviors in children and adolescents/young adults: A literature review.

Nielassoff, Emilie / Le Floch, Marine / Avril, Clémence / Gohier, Bénédicte / Duverger, Philippe / Riquin, Elise

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie

2023 Volume 30, Issue 8, Page(s) 607–616

Abstract: Background: Suicidal behaviors present a public health challenge in children and adolescents. Although the risk factors have long been well documented, protective factors have only been documented for a few years, and there has not been a literature ... ...

Abstract	Background: Suicidal behaviors present a public health challenge in children and adolescents. Although the risk factors have long been well documented, protective factors have only been documented for a few years, and there has not been a literature review concerning the suicidal behaviors of children and adolescents since 2006. Methods: Relevant articles were collected using the Medline/PubMed, Web of Science, and ScienceDirect databases. Studies meeting the following inclusion criteria were included: age of participants from 6 to 19 years, qualitative and quantitative cohort or case-control studies, multivariate analysis studies, and studies with significant results for at least one protective factor. The methodology used in this review is based on the PRISMA criteria. Results: A total of 26 studies were included in this review, which highlights various individual and environmental protective factors. The results were too heterogeneous to perform a meta-analysis, and therefore the discussion is in the form of a narrative summary. High-quality relationships with family and in the school environment were the most frequently found protective factors. The presence of positive links with peers, with other adults, and with the culture of origin was also noted. On an individual level, self-esteem, emotional intelligence, and particular coping abilities were found to be the most important protective factors. Conclusion: There are numerous important protective factors for suicidal behaviors in children and adolescents and also for adapting care to their needs. A future challenge will be to determine the best protective factors to be consolidated or strengthened using self-assessment tools that are already in use or being developed.
MeSH term(s)	Adolescent ; Adult ; Child ; Humans ; Young Adult ; Adaptation, Psychological ; Protective Factors ; Risk Factors ; Self Concept ; Suicidal Ideation
Language	English
Publishing date	2023-09-29
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	1181947-9
ISSN	1769-664X ; 0929-693X
ISSN (online)	1769-664X
ISSN	0929-693X
DOI	10.1016/j.arcped.2023.07.006
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Article ; Online: Risk factors of post-traumatic stress disorder after hospitalization in a pediatric intensive care unit: a systematic literature review.

de Pellegars, Alice / Cariou, Cindy / Le Floch, Marine / Duverger, Philippe / Boussicault, Gérald / Riquin, Elise

European child & adolescent psychiatry

2023

Abstract: The number of studies on post-traumatic stress disorder after hospitalization in a pediatric intensive care unit raised since 2004. The objective of this systematic review was to summarize and critically examine the literature about risk factors for ... ...

Abstract	The number of studies on post-traumatic stress disorder after hospitalization in a pediatric intensive care unit raised since 2004. The objective of this systematic review was to summarize and critically examine the literature about risk factors for these children to develop post-traumatic stress disorder following admission to an intensive care unit. The data sources were PubMed, Cochrane, Web of Science, PsycInfo, SUDOC, Scopus, and ScienceDirect. Studies were selected if they were in English or French and published between 01/01/2004 and 31/01/2022. Studies were excluded if patients were less than 1 month old and if no post-traumatic stress disorder was found. The internal validity and risk of bias were assessed using the National Institutes of Health Study Quality Assessment Tools for observational studies and the Ottawa Scale was used for the interventional study. The search yielded 523 results and 22 articles met inclusion criteria. Three common risk factors were identified from the data: parental post-traumatic stress disorder (especially in mothers), severity of illness and delusional memories. Internalizing behavior in children, acute parent and child stress, emergency admission and sepsis are also potential risk factors that require further investigation. The prevalence of this pathology is substantial (between 14 and 36%) and increasing awareness among pediatricians and psychologists seems necessary. Prevention programs are being studied to reduce the incidence of post-traumatic stress disorder in this population. Child and adolescent psychiatry liaison should collaborate with pediatric teams to support this objective.
Language	English
Publishing date	2023-02-05
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	1118299-4
ISSN	1435-165X ; 1018-8827 ; 1433-5719
ISSN (online)	1435-165X
ISSN	1018-8827 ; 1433-5719
DOI	10.1007/s00787-023-02141-8
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