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Article ; Online: Effect of apigenin on dynamin-related protein 1 in type 1 diabetic rats with cardiovascular complications.

Gamal Sherif, Sara / Tarek, Marwa / Gamal Sabry, Yasmine / Hassan Abou Ghalia, Azza

2023 Volume 898, Page(s) 148107

Abstract: Background and objective: Cardiovascular complications cause increased mortality rates among diabetics. The molecular mechanisms of aberrant mitochondrial dynamics in diabetes mellitus (DM) are not fully understood. Dynamin-related protein 1 (Drp1) is ... ...

Abstract	Background and objective: Cardiovascular complications cause increased mortality rates among diabetics. The molecular mechanisms of aberrant mitochondrial dynamics in diabetes mellitus (DM) are not fully understood. Dynamin-related protein 1 (Drp1) is thought to be a major regulator of mitochondrial fission. There is lack of studies that examined the relationship between apigenin and Drp1 expression in DM. Thus, the current study aimed to explore the expression of Drp1 in diabetic rats with cardiovascular complications, as well as to appraise the role of apigenin in modulating this expression. Methods: Twenty-eight adult male albino Wister rats were randomly and equally allocated into four groups: naive, streptozotocin-induced type 1 diabetic control and two apigenin-injected diabetic groups (early and late). Body weight, heart weight, blood pressure and ECG were recorded. Evaluation of blood glucose level, lipid profile and cardiac functions were measured. Determination of Drp1 mRNA expression, and histological examination of cardiac tissues from the four groups were performed. Results: Diabetic control rats developed decrease of body weight, increase of blood pressure, deterioration of the normal ECG pattern and upregulation of Drp1 mRNA expression in cardiac tissues. There was a significant correlation between the relative expression of Drp1 and all examined parameters. Apigenin-injection improved fasting blood glucose, lipid profile and cardiac function indicators (i.e., ECG parameters, CK-MB and troponin) as well as the cardiac histological structure. The decrease of Drp1 expression was more evident with early than with late apigenin-injection, however, without statistical significance. Conclusions: Increased level of Drp1 expression in diabetic rats may be involved in the pathogenesis of diabetic cardiovascular complications. The changes that occurred in response to apigenin injection highlight its potential ameliorative effect on the diabetic cardiovascular complications and pave the route for further investigations.
MeSH term(s)	Rats ; Male ; Animals ; Apigenin/pharmacology ; Blood Glucose/metabolism ; Diabetes Mellitus, Type 1/complications ; Diabetes Mellitus, Type 1/drug therapy ; Diabetes Mellitus, Experimental/complications ; Diabetes Mellitus, Experimental/metabolism ; Dynamins/genetics ; RNA, Messenger/genetics ; Body Weight ; Lipids/adverse effects
Chemical Substances	Apigenin (7V515PI7F6) ; Blood Glucose ; Dynamins (EC 3.6.5.5) ; RNA, Messenger ; Lipids
Language	English
Publishing date	2023-12-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2023.148107
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Article ; Online: Clinical Utility of Novel Fractional Flow Reserve Pullback for Individual Lesion Contribution in Serial Disease.

Modi, Bhavik N / Demir, Ozan M / Rahman, Haseeb / Ryan, Matthew / Abou Sherif, Sara / Ellis, Howard / Colombo, Antonio / Perera, Divaka

The Journal of invasive cardiology

2021 Volume 33, Issue 7, Page(s) E491–E496

Abstract: Objectives: Fractional flow reserve (FFR) pullback is frequently used to assess serially diseased arteries, but has been shown to be inaccurate due to physiological interaction between individual lesions. We evaluated the clinical utility of a novel ... ...

Abstract	Objectives: Fractional flow reserve (FFR) pullback is frequently used to assess serially diseased arteries, but has been shown to be inaccurate due to physiological interaction between individual lesions. We evaluated the clinical utility of a novel solution that improves estimation of true FFR contribution of each stenosis in the presence of serial disease. Methods: Ten interventional cardiologists were presented with tiered information for 18 elective patients with serial coronary disease and submitted revascularization strategies and assessment of lesion significance. Operators were first shown clinical and angiographic information only (Angio); then, conventional practice FFR (FFRnorm); and finally, pullback with corrected FFR contributions of each stenosis (FFRpred). Results: The treatment strategy agreement between operators was k=0.39, k=0.64, and k=0.77 using Angio, FFRnorm, and FFRpred, respectively (P<.001). Lesion significance uncertainty was 26%, 28%, and 3%, respectively. The number of stents per patient was 1.49 ± 0.57, 1.50 ± 0.57, and 1.3 ± 0.5, respectively (P<.001). In total, percutaneous coronary intervention (PCI) strategy changed in over 50% of cases analyzed, with participants opting for shorter stent length with FFRpred (29.5 ± 15.2 mm) compared with FFRnorm (34.1 ± 14.4 mm; P<.001) and Angio (34.6 ± 14.3; P=.04). This was accompanied by significantly less interobserver variability. Conclusion: The ability to quantify the contribution of individual lesions with the novel FFR pullback-based solution significantly increases operator confidence regarding PCI strategy, reduces heterogeneity in practice, and can reduce the planned number of stents and total stent length.
MeSH term(s)	Coronary Angiography ; Coronary Artery Disease/diagnosis ; Coronary Artery Disease/surgery ; Coronary Stenosis/diagnosis ; Coronary Stenosis/surgery ; Fractional Flow Reserve, Myocardial ; Humans ; Percutaneous Coronary Intervention ; Treatment Outcome
Language	English
Publishing date	2021-06-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	1154372-3
ISSN	1557-2501 ; 1042-3931
ISSN (online)	1557-2501
ISSN	1042-3931
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Zs.A 3701: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Investigating the potential of clinical and biochemical markers to differentiate between functional hypothalamic amenorrhoea and polycystic ovarian syndrome: A retrospective observational study.

Abou Sherif, Sara / Newman, Rebecca / Haboosh, Sara / Al-Sharefi, Ahmed / Papanikolaou, Nikoleta / Dimakopoulou, Anastasia / Webber, Lisa J / Abbara, Ali / Franks, Stephen / Dhillo, Waljit S / Jayasena, Channa N

Clinical endocrinology

2021 Volume 95, Issue 4, Page(s) 618–627

Abstract: Objectives: Functional hypothalamic amenorrhoea (FHA) is a common cause of amenorrhoea, but diagnosis can be challenging. The aim of this study was to investigate the clinical and biochemical features of FHA, compared to that of polycystic ovarian ... ...

Abstract	Objectives: Functional hypothalamic amenorrhoea (FHA) is a common cause of amenorrhoea, but diagnosis can be challenging. The aim of this study was to investigate the clinical and biochemical features of FHA, compared to that of polycystic ovarian syndrome (PCOS) and assess the diagnostic performance of the different parameters for differentiating the two conditions. Design and patients: This was a retrospective observational study. We analysed clinical and biochemical parameters of women diagnosed with FHA and PCOS following specialist assessment at the reproductive endocrine gynaecology clinic, St Mary's Hospital. Results: Compared with PCOS, women with FHA had significantly lower body mass index (BMI; 20.1 ± 2.9 vs. 31.1 ± 7.8 kg/m Conclusions: Our data provides quantification for diagnostic accuracy of clinical parameters to differentiate FHA from PCOS, namely low BMI, estradiol, LH and LH:FSH ratio. These data could help clinicians more reliably diagnose FHA in women with secondary amenorrhoea.
MeSH term(s)	Amenorrhea/diagnosis ; Biomarkers ; Female ; Follicle Stimulating Hormone ; Humans ; Luteinizing Hormone ; Polycystic Ovary Syndrome/diagnosis
Chemical Substances	Biomarkers ; Luteinizing Hormone (9002-67-9) ; Follicle Stimulating Hormone (9002-68-0)
Language	English
Publishing date	2021-08-09
Publishing country	England
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ZDB-ID	121745-8
ISSN	1365-2265 ; 0300-0664
ISSN (online)	1365-2265
ISSN	0300-0664
DOI	10.1111/cen.14571
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Article ; Online: Intra-coronary Imaging for the Evaluation of Plaque Modifications Induced by Drug Therapies for Secondary Prevention.

Kilic, Ismail Dogu / Fabris, Enrico / Kedhi, Elvin / Ghilencea, Liviu-Nicolae / Caiazzo, Gianluca / Sherif, Sara Abou / Di Mario, Carlo

Current atherosclerosis reports

2020 Volume 22, Issue 12, Page(s) 76

Abstract: Purpose of review: Patients diagnosed with coronary artery disease are at a high risk of subsequent cardiovascular events; therefore, secondary prevention in the form of therapeutic lifestyle changes, and drug therapies is vital. This article aims to ... ...

Abstract	Purpose of review: Patients diagnosed with coronary artery disease are at a high risk of subsequent cardiovascular events; therefore, secondary prevention in the form of therapeutic lifestyle changes, and drug therapies is vital. This article aims to review potential application of intra-coronary imaging for the evaluation of plaque modifications, induced by medications for secondary prevention for CAD. Recent findings: Intra-coronary imaging provides detailed information on the atherosclerotic plaque which is the primary pathological substrate for the recurrent ischemic cardiovascular events. These modalities can detect features associated with high risk and allow serial in vivo imaging of lesions. Therefore, intravascular imaging tools have been used in landmark studies and played a role in improving our understanding of the disease processes. Changes in size and plaque composition over time can be evaluated by these tools and may help understanding the impact of a treatment. Moreover, surrogate imaging end points can be used when testing new drugs for secondary prevention.
MeSH term(s)	Blood Component Removal/methods ; Cholesterol, HDL/blood ; Coronary Artery Disease/complications ; Coronary Artery Disease/diagnostic imaging ; Coronary Artery Disease/drug therapy ; Coronary Artery Disease/prevention & control ; Humans ; Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use ; PCSK9 Inhibitors ; Plaque, Atherosclerotic/complications ; Plaque, Atherosclerotic/diagnostic imaging ; Plaque, Atherosclerotic/drug therapy ; Plaque, Atherosclerotic/prevention & control ; Protease Inhibitors/therapeutic use ; Secondary Prevention ; Ultrasonography, Interventional/methods
Chemical Substances	Cholesterol, HDL ; Hydroxymethylglutaryl-CoA Reductase Inhibitors ; PCSK9 Inhibitors ; Protease Inhibitors ; PCSK9 protein, human (EC 3.4.21.-)
Language	English
Publishing date	2020-10-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2057369-8
ISSN	1534-6242 ; 1523-3804
ISSN (online)	1534-6242
ISSN	1523-3804
DOI	10.1007/s11883-020-00890-4
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Zs.A 5534: Show issues

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Article ; Online: Improvements in Sperm Motility Following Low- or High-Intensity Dietary Interventions in Men With Obesity.

The Journal of clinical endocrinology and metabolism

2023 Volume 109, Issue 2, Page(s) 449–460

Abstract: Introduction: Obesity increases risks of male infertility, but bariatric surgery does not improve semen quality. Recent uncontrolled studies suggest that a low-energy diet (LED) improves semen quality. Further evaluation within a randomized, controlled ... ...

Abstract	Introduction: Obesity increases risks of male infertility, but bariatric surgery does not improve semen quality. Recent uncontrolled studies suggest that a low-energy diet (LED) improves semen quality. Further evaluation within a randomized, controlled setting is warranted. Methods: Men with obesity (18-60 years) with normal sperm concentration (normal count) (n = 24) or oligozoospermia (n = 43) were randomized 1:1 to either 800 kcal/day LED for 16 weeks or control, brief dietary intervention (BDI) with 16 weeks' observation. Semen parameters were compared at baseline and 16 weeks. Results: Mean age of men with normal count was 39.4 ± 6.4 in BDI and 40.2 ± 9.6 years in the LED group. Mean age of men with oligozoospermia was 39.5 ± 7.5 in BDI and 37.7 ± 6.6 years in the LED group. LED caused more weight loss than BDI in men with normal count (14.4 vs 6.3 kg; P < .001) and men with oligozoospermia (17.6 vs 1.8 kg; P < .001). Compared with baseline, in men with normal count total motility (TM) increased 48 ± 17% to 60 ± 10% (P < .05) after LED, and 52 ± 8% to 61 ± 6% (P < .0001) after BDI; progressive motility (PM) increased 41 ± 16% to 53 ± 10% (P < .05) after LED, and 45 ± 8% to 54 ± 65% (P < .001) after BDI. In men with oligozoospermia compared with baseline, TM increased 35% [26] to 52% [16] (P < .05) after LED, and 43% [28] to 50% [23] (P = .0587) after BDI; PM increased 29% [23] to 46% [18] (P < .05) after LED, and 33% [25] to 44% [25] (P < .05) after BDI. No differences in postintervention TM or PM were observed between LED and BDI groups in men with normal count or oligozoospermia. Conclusion: LED or BDI may be sufficient to improve sperm motility in men with obesity. The effects of paternal dietary intervention on fertility outcomes requires investigation.
MeSH term(s)	Male ; Humans ; Semen Analysis ; Sperm Motility ; Oligospermia ; Semen ; Sperm Count ; Infertility, Male/etiology ; Spermatozoa ; Obesity/complications ; Obesity/surgery
Language	English
Publishing date	2023-09-01
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article
ZDB-ID	3029-6
ISSN	1945-7197 ; 0021-972X
ISSN (online)	1945-7197
ISSN	0021-972X
DOI	10.1210/clinem/dgad523
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Article ; Online: Fibromatosis of the Sigmoid Colon With CTNNB1 (β-Catenin) Gene Mutation, Arising at the Site of Ileocolic Anastomosis for Resection of Gastrointestinal Stromal Tumor.

Thway, Khin / Abou Sherif, Sara / Riddell, Angela M / Mudan, Satvinder

International journal of surgical pathology

2016 Volume 24, Issue 3, Page(s) 264–268

Abstract: We describe a case of intra-abdominal fibromatosis, which occurred in a 44-year-old woman who had a previous history of gastrointestinal stromal tumor (GIST) of the sigmoid mesocolon, which was treated with imatinib and resection. A mass was detected at ... ...

Abstract	We describe a case of intra-abdominal fibromatosis, which occurred in a 44-year-old woman who had a previous history of gastrointestinal stromal tumor (GIST) of the sigmoid mesocolon, which was treated with imatinib and resection. A mass was detected at the site of ileocolic anastomosis of the previous small bowel resection and sigmoid colectomy, nearly 3 years later. Clinically, this was suspected to represent recurrent GIST and was excised, but histology and mutational analysis showed desmoid-type fibromatosis with a mutation in codon 41 of exon 3 of the CTNNB1 (β-catenin) gene. The occurrence of fibromatosis at the site of excision of GIST is very rare, but its recognition is important as the treatment of the two neoplasms differs significantly. As imaging cannot reliably distinguish between these 2 entities, histological diagnosis is crucial for correct clinical management.
MeSH term(s)	Adult ; Anastomosis, Surgical/adverse effects ; DNA Mutational Analysis ; Female ; Fibroma/genetics ; Fibroma/pathology ; Gastrointestinal Stromal Tumors/surgery ; Humans ; Mutation ; Neoplasms, Second Primary/etiology ; Neoplasms, Second Primary/pathology ; Sigmoid Neoplasms/genetics ; Sigmoid Neoplasms/pathology ; beta Catenin/genetics
Chemical Substances	CTNNB1 protein, human ; beta Catenin
Language	English
Publishing date	2016-05
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	1336393-1
ISSN	1940-2465 ; 1066-8969
ISSN (online)	1940-2465
ISSN	1066-8969
DOI	10.1177/1066896915620012
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Article: Coronary Artery Aneurysms: A Review of the Epidemiology, Pathophysiology, Diagnosis, and Treatment.

Abou Sherif, Sara / Ozden Tok, Ozge / Taşköylü, Özgür / Goktekin, Omer / Kilic, Ismail Dogu

Frontiers in cardiovascular medicine

2017 Volume 4, Page(s) 24

Abstract: Coronary artery aneurysms (CAAs) are uncommon and describe a localized dilatation of a coronary artery segment more than 1.5-fold compared with adjacent normal segments. The incidence of CAAs varies from 0.3 to 5.3%. Ever since the dawn of the ... ...

Abstract	Coronary artery aneurysms (CAAs) are uncommon and describe a localized dilatation of a coronary artery segment more than 1.5-fold compared with adjacent normal segments. The incidence of CAAs varies from 0.3 to 5.3%. Ever since the dawn of the interventional era, CAAs have been increasingly diagnosed on coronary angiography. Causative factors include atherosclerosis, Takayasu arteritis, congenital disorders, Kawasaki disease (KD), and percutaneous coronary intervention. The natural history of CAAs remains unclear; however, several recent studies have postulated the underlying molecular mechanisms of CAAs, and genome-wide association studies have revealed several genetic predispositions to CAA. Controversies persist regarding the management of CAAs, and emerging findings support the importance of an early diagnosis in patients predisposed to CAAs, such as in children with KD. This review aims to summarize the present knowledge of CAAs and collate the recent advances regarding the epidemiology, etiology, pathophysiology, diagnosis, and treatment of this disease.
Language	English
Publishing date	2017-05-05
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2781496-8
ISSN	2297-055X
ISSN	2297-055X
DOI	10.3389/fcvm.2017.00024
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Article ; Online: Is heart rate response a reliable marker of adenosine-induced coronary hyperemia?

Modi, Bhavik N / Rahman, Haseeb / Sherif, Sara Abou / Ellis, Howard / Eruslanova, Kseniia / Chiribiri, Amedeo / Perera, Divaka

The international journal of cardiovascular imaging

2018 Volume 34, Issue 7, Page(s) 1117–1125

Abstract: Introduction Growing evidence supports ischemia-guided management of chest pain, with invasive and non-invasive tests reliant upon achieving adenosine-induced coronary hyperemia (defined as increased blood flow to an organ's perfusion bed). In the non- ... ...

Abstract	Introduction Growing evidence supports ischemia-guided management of chest pain, with invasive and non-invasive tests reliant upon achieving adenosine-induced coronary hyperemia (defined as increased blood flow to an organ's perfusion bed). In the non-invasive setting, surrogate markers of hyperemia, such as increases in heart rate, are often used, despite not being formally validated. We tested whether heart rate and other non-invasive indices are reliable markers of coronary hyperemia. Methods The first part involved Doppler flow-based validation of the best pressure-wire markers of hyperemia in 53 patients. Subsequently, using these validated pressure-derived parameters, 265 pressure-wire traces were analysed to determine whether heart rate and other non-invasive parameters correlated with hyperemia. Results In the flow derivation cohort, the best determinant of hyperemia came from having 2 out of 3 of: (1) Ventriculisation of the distal pressure waveform, (2) disappearance of distal dicrotic pressure notch, (3) separation of mean aortic and distal pressures. Within the 244 patients demonstrating hyperemia, non-invasive markers of hyperemia, such as change in heart rate (p = 0.77), blood pressure (p = 0.60) and rate-pressure product (p = 0.86), were poor correlates of coronary hyperemia, with only 37.3% demonstrating a ≥ 10% increase in heart rate that is commonly used to adjudge adenosine-induced hyperemia in the non-invasive setting. Conclusions We demonstrate, by correlation with Doppler-flow data, a validated method of identifying coronary hyperemia within the catheter laboratory using the pressure-wire. We subsequently show that non-invasive parameters, such as heart rate change, are poor predictors of coronary hyperemia during stress imaging protocols that rely upon achieving adenosine-induced hyperemia.
MeSH term(s)	Adenosine ; Aged ; Biomarkers ; Cardiac Imaging Techniques ; Coronary Circulation/drug effects ; Coronary Circulation/physiology ; Coronary Disease/complications ; Coronary Disease/diagnosis ; Coronary Disease/physiopathology ; Coronary Vessels/diagnostic imaging ; Coronary Vessels/drug effects ; Coronary Vessels/physiopathology ; Echocardiography, Doppler, Color/methods ; Echocardiography, Stress/methods ; Female ; Heart Rate/drug effects ; Heart Rate/physiology ; Humans ; Hyperemia/chemically induced ; Hyperemia/diagnostic imaging ; Hyperemia/physiopathology ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Myocardial Ischemia/diagnosis ; Myocardial Ischemia/etiology ; Myocardial Ischemia/physiopathology ; Predictive Value of Tests ; Retrospective Studies ; Tachycardia/chemically induced ; Vasodilator Agents
Chemical Substances	Biomarkers ; Vasodilator Agents ; Adenosine (K72T3FS567)
Language	English
Publishing date	2018-02-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2055311-0
ISSN	1875-8312 ; 1573-0743 ; 1569-5794 ; 0167-9899
ISSN (online)	1875-8312 ; 1573-0743
ISSN	1569-5794 ; 0167-9899
DOI	10.1007/s10554-018-1309-1
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Zs.A 2104: Show issues

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Article ; Online: Coronary covered stents.

Kilic, Ismail Dogu / Fabris, Enrico / Serdoz, Roberta / Caiazzo, Gianluca / Foin, Nicolas / Abou-Sherif, Sara / Di Mario, Carlo

EuroIntervention : journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology

2016 Volume 12, Issue 10, Page(s) 1288–1295

Abstract: Covered stents offer an effective bail-out strategy in vessel perforations, are an alternative to surgery for the exclusion of coronary aneurysms, and have a potential role in the treatment of friable embolisation-prone plaques. The aim of this ... ...

Abstract	Covered stents offer an effective bail-out strategy in vessel perforations, are an alternative to surgery for the exclusion of coronary aneurysms, and have a potential role in the treatment of friable embolisation-prone plaques. The aim of this manuscript is to offer an overview of currently available platforms and to report results obtained in prior studies.
MeSH term(s)	Angioplasty, Balloon, Coronary/methods ; Coronary Aneurysm/surgery ; Coronary Vessels/surgery ; Humans ; Stents ; Treatment Outcome ; Vascular System Injuries/surgery
Language	English
Publishing date	2016--20
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	2457174-X
ISSN	1969-6213 ; 1774-024X
ISSN (online)	1969-6213
ISSN	1774-024X
DOI	10.4244/EIJV12I10A210
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Article: TAVI: New trials and registries offer further welcome evidence - U.S. CoreValve, CHOICE, and GARY.

Pighi, Michele / Serdoz, Roberta / Kilic, Ismail Dogu / Sherif, Sara Abou / Lindsay, Alistair / Di Mario, Carlo

Global cardiology science & practice

2014 Volume 2014, Issue 1, Page(s) 78–87

Abstract: The introduction of transcatheter aortic valve implantation (TAVI) has resulted in a paradigm shift in the treatment of patients with severe aortic stenosis. Data from the recent U.S CoreValve Trial suggest, for the first time, that TAVI is associated ... ...

Abstract	The introduction of transcatheter aortic valve implantation (TAVI) has resulted in a paradigm shift in the treatment of patients with severe aortic stenosis. Data from the recent U.S CoreValve Trial suggest, for the first time, that TAVI is associated with a significantly higher rate of survival at one year compared to surgical aortic valve replacement (SAVR) in the treatment of high-risk patients affected by severe aortic stenosis. The present review discusses this study and the current evidence about TAVI, for the treatment of severe aortic stenosis, from major trials and real world registries.
Language	English
Publishing date	2014-01-29
Publishing country	Qatar
Document type	Journal Article
ZDB-ID	2738381-7
ISSN	2305-7823
ISSN	2305-7823
DOI	10.5339/gcsp.2014.12
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