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Article ; Online: Coronavirus Pseudotypes for All Circulating Human Coronaviruses for Quantification of Cross-Neutralizing Antibody Responses.

Sampson, Alexander Thomas / Heeney, Jonathan / Cantoni, Diego / Ferrari, Matteo / Sans, Maria Suau / George, Charlotte / Di Genova, Cecilia / Mayora Neto, Martin / Einhauser, Sebastian / Asbach, Benedikt / Wagner, Ralf / Baxendale, Helen / Temperton, Nigel / Carnell, George

Viruses

2021 Volume 13, Issue 8

Abstract: The novel coronavirus SARS-CoV-2 is the seventh identified human coronavirus. Understanding the extent of pre-existing immunity induced by seropositivity to endemic seasonal coronaviruses and the impact of cross-reactivity on COVID-19 disease progression ...

Abstract	The novel coronavirus SARS-CoV-2 is the seventh identified human coronavirus. Understanding the extent of pre-existing immunity induced by seropositivity to endemic seasonal coronaviruses and the impact of cross-reactivity on COVID-19 disease progression remains a key research question in immunity to SARS-CoV-2 and the immunopathology of COVID-2019 disease. This paper describes a panel of lentiviral pseudotypes bearing the spike (S) proteins for each of the seven human coronaviruses (HCoVs), generated under similar conditions optimized for high titre production allowing a high-throughput investigation of antibody neutralization breadth. Optimal production conditions and most readily available permissive target cell lines were determined for spike-mediated entry by each HCoV pseudotype: SARS-CoV-1, SARS-CoV-2 and HCoV-NL63 best transduced HEK293T/17 cells transfected with ACE2 and TMPRSS2, HCoV-229E and MERS-CoV preferentially entered HUH7 cells, and CHO cells were most permissive for the seasonal betacoronavirus HCoV-HKU1. Entry of ACE2 using pseudotypes was enhanced by ACE2 and TMPRSS2 expression in target cells, whilst TMPRSS2 transfection rendered HEK293T/17 cells permissive for HCoV-HKU1 and HCoV-OC43 entry. Additionally, pseudotype viruses were produced bearing additional coronavirus surface proteins, including the SARS-CoV-2 Envelope (E) and Membrane (M) proteins and HCoV-OC43/HCoV-HKU1 Haemagglutinin-Esterase (HE) proteins. This panel of lentiviral pseudotypes provides a safe, rapidly quantifiable and high-throughput tool for serological comparison of pan-coronavirus neutralizing responses; this can be used to elucidate antibody dynamics against individual coronaviruses and the effects of antibody cross-reactivity on clinical outcome following natural infection or vaccination.
MeSH term(s)	Animals ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; Broadly Neutralizing Antibodies/blood ; Broadly Neutralizing Antibodies/immunology ; COVID-19/immunology ; Cell Line ; Coronavirus/immunology ; Coronavirus 229E, Human/immunology ; Coronavirus 229E, Human/physiology ; Coronavirus NL63, Human/immunology ; Coronavirus NL63, Human/physiology ; Coronavirus OC43, Human/immunology ; Coronavirus OC43, Human/physiology ; Cross Reactions ; Humans ; Lentivirus/genetics ; Middle East Respiratory Syndrome Coronavirus/immunology ; Middle East Respiratory Syndrome Coronavirus/physiology ; Neutralization Tests ; Plasmids ; SARS-CoV-2/immunology ; SARS-CoV-2/physiology ; Spike Glycoprotein, Coronavirus/immunology ; Transfection ; Virus Internalization
Chemical Substances	Antibodies, Viral ; Broadly Neutralizing Antibodies ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2021-08-10
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v13081579
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Article: Coronavirus Pseudotypes for All Circulating Human Coronaviruses for Quantification of Cross-Neutralizing Antibody Responses

Viruses. 2021 Aug. 10, v. 13, no. 8

2021

Abstract	The novel coronavirus SARS-CoV-2 is the seventh identified human coronavirus. Understanding the extent of pre-existing immunity induced by seropositivity to endemic seasonal coronaviruses and the impact of cross-reactivity on COVID-19 disease progression remains a key research question in immunity to SARS-CoV-2 and the immunopathology of COVID-2019 disease. This paper describes a panel of lentiviral pseudotypes bearing the spike (S) proteins for each of the seven human coronaviruses (HCoVs), generated under similar conditions optimized for high titre production allowing a high-throughput investigation of antibody neutralization breadth. Optimal production conditions and most readily available permissive target cell lines were determined for spike-mediated entry by each HCoV pseudotype: SARS-CoV-1, SARS-CoV-2 and HCoV-NL63 best transduced HEK293T/17 cells transfected with ACE2 and TMPRSS2, HCoV-229E and MERS-CoV preferentially entered HUH7 cells, and CHO cells were most permissive for the seasonal betacoronavirus HCoV-HKU1. Entry of ACE2 using pseudotypes was enhanced by ACE2 and TMPRSS2 expression in target cells, whilst TMPRSS2 transfection rendered HEK293T/17 cells permissive for HCoV-HKU1 and HCoV-OC43 entry. Additionally, pseudotype viruses were produced bearing additional coronavirus surface proteins, including the SARS-CoV-2 Envelope (E) and Membrane (M) proteins and HCoV-OC43/HCoV-HKU1 Haemagglutinin-Esterase (HE) proteins. This panel of lentiviral pseudotypes provides a safe, rapidly quantifiable and high-throughput tool for serological comparison of pan-coronavirus neutralizing responses; this can be used to elucidate antibody dynamics against individual coronaviruses and the effects of antibody cross-reactivity on clinical outcome following natural infection or vaccination.
Keywords	Betacoronavirus 1 ; COVID-19 infection ; Severe acute respiratory syndrome coronavirus ; Severe acute respiratory syndrome coronavirus 2 ; broadly neutralizing antibodies ; cross reaction ; disease progression ; humans ; immunopathology ; neutralization ; seroprevalence ; transfection ; vaccination
Language	English
Dates of publication	2021-0810
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v13081579
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Article ; Online: Prehistoric baseline reveals substantial decline of oyster reef condition in a Gulf of Mexico conservation priority area.

Hesterberg, Stephen G / Herbert, Gregory S / Pluckhahn, Thomas J / Harke, Ryan M / Al-Qattan, Nasser M / Duke, C Trevor / Moore, Evan W / Smith, Megan E / Delgado, Alexander C / Sampson, Christina P

Biology letters

2020 Volume 16, Issue 2, Page(s) 20190865

Abstract: The Gulf of Mexico (GoM) is home to the world's largest remaining wild oyster fisheries, but baseline surveys needed to assess habitat condition are recent and may represent an already-shifted reference state. Here, we use prehistoric oysters from ... ...

Abstract	The Gulf of Mexico (GoM) is home to the world's largest remaining wild oyster fisheries, but baseline surveys needed to assess habitat condition are recent and may represent an already-shifted reference state. Here, we use prehistoric oysters from archaeological middens to show that oyster size, an indicator of habitat function and population resilience, declined prior to the earliest assessments of reef condition in an area of the GoM previously considered pristine. Stable isotope sclerochronlogy reveals extirpation of colossal oysters occurred through truncated life history and slowed growth. More broadly, our study suggests that management strategies affected by shifting baselines may overestimate resilience and perpetuate practices that risk irreversible decline.
MeSH term(s)	Animals ; Crassostrea ; Ecosystem ; Fisheries ; Gulf of Mexico ; Mexico
Language	English
Publishing date	2020-02-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2135022-X
ISSN	1744-957X ; 1744-9561
ISSN (online)	1744-957X
ISSN	1744-9561
DOI	10.1098/rsbl.2019.0865
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Article ; Online: Risk factors for the transition from suicide ideation to suicide attempt: Results from the Army Study to Assess Risk and Resilience in Servicemembers (Army STARRS).

Nock, Matthew K / Millner, Alexander J / Joiner, Thomas E / Gutierrez, Peter M / Han, Georges / Hwang, Irving / King, Andrew / Naifeh, James A / Sampson, Nancy A / Zaslavsky, Alan M / Stein, Murray B / Ursano, Robert J / Kessler, Ronald C

Journal of abnormal psychology

2018 Volume 127, Issue 2, Page(s) 139–149

Abstract: Prior research has shown that most known risk factors for suicide attempts in the general population actually predict suicide ideation rather than attempts among ideators. Yet clinical interest in predicting suicide attempts often involves the evaluation ...

Abstract	Prior research has shown that most known risk factors for suicide attempts in the general population actually predict suicide ideation rather than attempts among ideators. Yet clinical interest in predicting suicide attempts often involves the evaluation of risk among patients with ideation. We examined a number of characteristics of suicidal thoughts hypothesized to predict incident attempts in a retrospective analysis of lifetime ideators (N = 3,916) drawn from a large (N = 29,982), representative sample of United States Army soldiers. The most powerful predictors of first nonfatal lifetime suicide attempt in a multivariate model controlling for previously known predictors (e.g., demographics, mental disorders) were: recent onset of ideation, presence and recent onset of a suicide plan, low controllability of suicidal thoughts, extreme risk-taking or "tempting fate," and failure to answer questions about the characteristics of one's suicidal thoughts. A predictive model using these risk factors had strong accuracy (area under the curve [AUC] = .93), with 66.2% of all incident suicide attempts occurring among the 5% of soldiers with highest composite predicted risk. This high concentration of risk in this retrospective study suggests that a useful clinical decision support model could be constructed from prospective data to identify those with highest risk of subsequent suicide attempt. (PsycINFO Database Record
MeSH term(s)	Female ; Humans ; Male ; Military Personnel/psychology ; ROC Curve ; Retrospective Studies ; Risk Factors ; Suicidal Ideation ; Suicide, Attempted/prevention & control ; Suicide, Attempted/psychology ; Suicide, Attempted/statistics & numerical data ; United States
Language	English
Publishing date	2018-03-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	3127-6
ISSN	1939-1846 ; 0021-843X ; 0096-851X ; 0145-2339 ; 0145-2347
ISSN (online)	1939-1846
ISSN	0021-843X ; 0096-851X ; 0145-2339 ; 0145-2347
DOI	10.1037/abn0000317
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Article ; Online: Patterns and predictors of persistence of suicide ideation: Results from the Army Study to Assess Risk and Resilience in Servicemembers (Army STARRS).

Nock, Matthew K / Han, Georges / Millner, Alexander J / Gutierrez, Peter M / Joiner, Thomas E / Hwang, Irving / King, Andrew / Naifeh, James A / Sampson, Nancy A / Zaslavsky, Alan M / Stein, Murray B / Ursano, Robert J / Kessler, Ronald C

Journal of abnormal psychology

2018 Volume 127, Issue 7, Page(s) 650–658

Abstract: Persistent suicide ideation (SI) is known to be a risk factor for subsequent suicidal behaviors. Reducing SI persistence among people with a history of SI consequently might be a useful target for preventive intervention; however, basic information is ... ...

Abstract	Persistent suicide ideation (SI) is known to be a risk factor for subsequent suicidal behaviors. Reducing SI persistence among people with a history of SI consequently might be a useful target for preventive intervention; however, basic information is lacking about patterns and predictors of SI persistence. We report preliminary retrospective data on annual SI persistence in a representative sample of 3,501 U.S. Army soldiers with lifetime SI from the Army Study to Assess Risk and Resilience in Servicemembers (Army STARRS). Reports about age-of-onset and number of years with SI were used to estimate two definitions of persistence: persistence beyond year-of-onset and proportional annual persistence (i.e., percentage of years with SI since year-of-onset). Results revealed that for 47.8% of respondents with lifetime SI, their SI did not persist beyond the year-of-onset. For the 52.2% whose SI did persist beyond the year-of-onset, the median (interquartile range) proportional annual persistence was 33% (17-67%). Significant predictors of increased persistence were different for respondents with preenlistment SI onset (prior histories of attention-deficit/hyperactivity disorder [ADHD], bipolar disorder, and panic disorder) and postenlistment SI onset (male, combat support military occupation specialty, prior histories of ADHD, panic disorder, and posttraumatic stress disorder). These predictors of persistence are different from the predictors of SI onset, suggesting that secondary preventive interventions to reduce SI persistence may need to focus on different factors than primary preventive interventions to reduce SI onset. (PsycINFO Database Record (c) 2018 APA, all rights reserved).
MeSH term(s)	Adult ; Age Factors ; Attention Deficit Disorder with Hyperactivity/psychology ; Female ; Humans ; Male ; Military Personnel/psychology ; Panic Disorder/psychology ; Resilience, Psychological ; Retrospective Studies ; Risk Assessment ; Risk Factors ; Sex Factors ; Stress Disorders, Post-Traumatic/psychology ; Suicidal Ideation
Language	English
Publishing date	2018-10-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	3127-6
ISSN	1939-1846 ; 0021-843X ; 0096-851X ; 0145-2339 ; 0145-2347
ISSN (online)	1939-1846
ISSN	0021-843X ; 0096-851X ; 0145-2339 ; 0145-2347
DOI	10.1037/abn0000379
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Article ; Online: Updated International Tuberous Sclerosis Complex Diagnostic Criteria and Surveillance and Management Recommendations.

Northrup, Hope / Aronow, Mary E / Bebin, E Martina / Bissler, John / Darling, Thomas N / de Vries, Petrus J / Frost, Michael D / Fuchs, Zoë / Gosnell, Elizabeth S / Gupta, Nishant / Jansen, Anna C / Jóźwiak, Sergiusz / Kingswood, J Chris / Knilans, Timothy K / McCormack, Francis X / Pounders, Ashley / Roberds, Steven L / Rodriguez-Buritica, David F / Roth, Jonathan /

Sampson, Julian R / Sparagana, Steven / Thiele, Elizabeth Anne / Weiner, Howard L / Wheless, James W / Towbin, Alexander J / Krueger, Darcy A

Pediatric neurology

2021 Volume 123, Page(s) 50–66

Abstract: Background: Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disease affecting multiple body systems with wide variability in presentation. In 2013, Pediatric Neurology published articles outlining updated diagnostic criteria and ... ...

Abstract	Background: Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disease affecting multiple body systems with wide variability in presentation. In 2013, Pediatric Neurology published articles outlining updated diagnostic criteria and recommendations for surveillance and management of disease manifestations. Advances in knowledge and approvals of new therapies necessitated a revision of those criteria and recommendations. Methods: Chairs and working group cochairs from the 2012 International TSC Consensus Group were invited to meet face-to-face over two days at the 2018 World TSC Conference on July 25 and 26 in Dallas, TX, USA. Before the meeting, working group cochairs worked with group members via e-mail and telephone to (1) review TSC literature since the 2013 publication, (2) confirm or amend prior recommendations, and (3) provide new recommendations as required. Results: Only two changes were made to clinical diagnostic criteria reported in 2013: "multiple cortical tubers and/or radial migration lines" replaced the more general term "cortical dysplasias," and sclerotic bone lesions were reinstated as a minor criterion. Genetic diagnostic criteria were reaffirmed, including highlighting recent findings that some individuals with TSC are genetically mosaic for variants in TSC1 or TSC2. Changes to surveillance and management criteria largely reflected increased emphasis on early screening for electroencephalographic abnormalities, enhanced surveillance and management of TSC-associated neuropsychiatric disorders, and new medication approvals. Conclusions: Updated TSC diagnostic criteria and surveillance and management recommendations presented here should provide an improved framework for optimal care of those living with TSC and their families.
MeSH term(s)	Child ; Consensus ; Humans ; Practice Guidelines as Topic ; Tuberous Sclerosis/diagnosis ; Tuberous Sclerosis/therapy
Language	English
Publishing date	2021-07-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	639164-3
ISSN	1873-5150 ; 0887-8994
ISSN (online)	1873-5150
ISSN	0887-8994
DOI	10.1016/j.pediatrneurol.2021.07.011
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Article ; Online: G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Prentzell, Mirja Tamara / Rehbein, Ulrike / Cadena Sandoval, Marti / De Meulemeester, Ann-Sofie / Baumeister, Ralf / Brohée, Laura / Berdel, Bianca / Bockwoldt, Mathias / Carroll, Bernadette / Chowdhury, Suvagata Roy / von Deimling, Andreas / Demetriades, Constantinos / Figlia, Gianluca / de Araujo, Mariana Eca Guimaraes / Heberle, Alexander M / Heiland, Ines / Holzwarth, Birgit / Huber, Lukas A / Jaworski, Jacek /

Kedra, Magdalena / Kern, Katharina / Kopach, Andrii / Korolchuk, Viktor I / van 't Land-Kuper, Ineke / Macias, Matylda / Nellist, Mark / Palm, Wilhelm / Pusch, Stefan / Ramos Pittol, Jose Miguel / Reil, Michèle / Reintjes, Anja / Reuter, Friederike / Sampson, Julian R / Scheldeman, Chloë / Siekierska, Aleksandra / Stefan, Eduard / Teleman, Aurelio A / Thomas, Laura E / Torres-Quesada, Omar / Trump, Saskia / West, Hannah D / de Witte, Peter / Woltering, Sandra / Yordanov, Teodor E / Zmorzynska, Justyna / Opitz, Christiane A / Thedieck, Kathrin

Cell

2021 Volume 184, Issue 3, Page(s) 655–674.e27

Abstract: Ras GTPase-activating protein-binding proteins 1 and 2 (G3BP1 and G3BP2, respectively) are widely recognized as core components of stress granules (SGs). We report that G3BPs reside at the cytoplasmic surface of lysosomes. They act in a non-redundant ... ...

Abstract	Ras GTPase-activating protein-binding proteins 1 and 2 (G3BP1 and G3BP2, respectively) are widely recognized as core components of stress granules (SGs). We report that G3BPs reside at the cytoplasmic surface of lysosomes. They act in a non-redundant manner to anchor the tuberous sclerosis complex (TSC) protein complex to lysosomes and suppress activation of the metabolic master regulator mechanistic target of rapamycin complex 1 (mTORC1) by amino acids and insulin. Like the TSC complex, G3BP1 deficiency elicits phenotypes related to mTORC1 hyperactivity. In the context of tumors, low G3BP1 levels enhance mTORC1-driven breast cancer cell motility and correlate with adverse outcomes in patients. Furthermore, G3bp1 inhibition in zebrafish disturbs neuronal development and function, leading to white matter heterotopia and neuronal hyperactivity. Thus, G3BPs are not only core components of SGs but also a key element of lysosomal TSC-mTORC1 signaling.
MeSH term(s)	Adaptor Proteins, Signal Transducing/metabolism ; Amino Acid Sequence ; Animals ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Cell Movement/drug effects ; Cytoplasmic Granules/drug effects ; Cytoplasmic Granules/metabolism ; DNA Helicases/chemistry ; DNA Helicases/metabolism ; Evolution, Molecular ; Female ; Humans ; Insulin/pharmacology ; Lysosomal Membrane Proteins/metabolism ; Lysosomes/drug effects ; Lysosomes/metabolism ; Mechanistic Target of Rapamycin Complex 1/metabolism ; Neurons/drug effects ; Neurons/metabolism ; Phenotype ; Poly-ADP-Ribose Binding Proteins/chemistry ; Poly-ADP-Ribose Binding Proteins/metabolism ; RNA Helicases/chemistry ; RNA Helicases/metabolism ; RNA Recognition Motif Proteins/chemistry ; RNA Recognition Motif Proteins/metabolism ; RNA-Binding Proteins/metabolism ; Rats, Wistar ; Signal Transduction/drug effects ; Tuberous Sclerosis/metabolism ; Zebrafish/metabolism ; Rats
Chemical Substances	Adaptor Proteins, Signal Transducing ; G3BP2 protein, human ; Insulin ; Lysosomal Membrane Proteins ; Poly-ADP-Ribose Binding Proteins ; RNA Recognition Motif Proteins ; RNA-Binding Proteins ; Mechanistic Target of Rapamycin Complex 1 (EC 2.7.11.1) ; DNA Helicases (EC 3.6.4.-) ; G3BP1 protein, human (EC 3.6.4.12) ; RNA Helicases (EC 3.6.4.13)
Language	English
Publishing date	2021-01-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	187009-9
ISSN	1097-4172 ; 0092-8674
ISSN (online)	1097-4172
ISSN	0092-8674
DOI	10.1016/j.cell.2020.12.024
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Article ; Online: Endothelial Expression of Scavenger Receptor Class B, Type I Protects against Development of Atherosclerosis in Mice.

Vaisman, Boris L / Vishnyakova, Tatyana G / Freeman, Lita A / Amar, Marcelo J / Demosky, Stephen J / Liu, Chengyu / Stonik, John A / Sampson, Maureen L / Pryor, Milton / Bocharov, Alexander V / Eggerman, Thomas L / Patterson, Amy P / Remaley, Alan T

BioMed research international

2015 Volume 2015, Page(s) 607120

Abstract: The role of scavenger receptor class B, type I (SR-BI) in endothelial cells (EC) was examined in several novel transgenic mouse models expressing SR-BI in endothelium of mice with normal C57Bl6/N, apoE-KO, or Scarb1-KO backgrounds. Mice were also created ...

Abstract	The role of scavenger receptor class B, type I (SR-BI) in endothelial cells (EC) was examined in several novel transgenic mouse models expressing SR-BI in endothelium of mice with normal C57Bl6/N, apoE-KO, or Scarb1-KO backgrounds. Mice were also created expressing SR-BI exclusively in endothelium and liver. Endothelial expression of the Tie2-Scarb1 transgene had no significant effect on plasma lipoprotein levels in mice on a normal chow diet but on an atherogenic diet, significantly decreased plasma cholesterol levels, increased plasma HDL cholesterol (HDL-C) levels, and protected mice against atherosclerosis. In 8-month-old apoE-KO mice fed a normal chow diet, the Tie2-Scarb1 transgene decreased aortic lesions by 24%. Mice expressing SR-BI only in EC and liver had a 1.5 ± 0.1-fold increase in plasma cholesterol compared to mice synthesizing SR-BI only in liver. This elevation was due mostly to increased HDL-C. In EC culture studies, SR-BI was found to be present in both basolateral and apical membranes but greater cellular uptake of cholesterol from HDL was found in the basolateral compartment. In summary, enhanced expression of SR-BI in EC resulted in a less atherogenic lipoprotein profile and decreased atherosclerosis, suggesting a possible role for endothelial SR-BI in the flux of cholesterol across EC.
MeSH term(s)	Animals ; Aorta/chemistry ; Aorta/cytology ; Aorta/metabolism ; Atherosclerosis/metabolism ; Atherosclerosis/prevention & control ; Cholesterol/blood ; Endothelium, Vascular/chemistry ; Endothelium, Vascular/metabolism ; Female ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Models, Biological ; Scavenger Receptors, Class B/analysis ; Scavenger Receptors, Class B/genetics ; Scavenger Receptors, Class B/metabolism
Chemical Substances	Scarb1 protein, mouse ; Scavenger Receptors, Class B ; Cholesterol (97C5T2UQ7J)
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2015/607120
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Article ; Online: Remotely Sensed Winds and Wind Stresses for Marine Forecasting and Ocean Modeling

Mark A. Bourassa / Thomas Meissner / Ivana Cerovecki / Paul S. Chang / Xiaolong Dong / Giovanna De Chiara / Craig Donlon / Dmitry S. Dukhovskoy / Jocelyn Elya / Alexander Fore / Melanie R. Fewings / Ralph C. Foster / Sarah T. Gille / Brian K. Haus / Svetla Hristova-Veleva / Heather M. Holbach / Zorana Jelenak / John A. Knaff / Sven A. Kranz /

Andrew Manaster / Matthew Mazloff / Carl Mears / Alexis Mouche / Marcos Portabella / Nicolas Reul / Lucrezia Ricciardulli / Ernesto Rodriguez / Charles Sampson / Daniel Solis / Ad Stoffelen / Michael R. Stukel / Bryan Stiles / David Weissman / Frank Wentz

Frontiers in Marine Science, Vol

2019 Volume 6

Abstract: Strengths and weakness of remotely sensed winds are discussed, along with the current capabilities for remotely sensing winds and stress. Future missions are briefly mentioned. The observational needs for a wide range of wind and stress applications are ... ...

Abstract	Strengths and weakness of remotely sensed winds are discussed, along with the current capabilities for remotely sensing winds and stress. Future missions are briefly mentioned. The observational needs for a wide range of wind and stress applications are provided. These needs strongly support a short list of desired capabilities of future missions and constellations.
Keywords	satellite ; wind ; stress ; ocean ; requirements ; Science ; Q ; General. Including nature conservation ; geographical distribution ; QH1-199.5
Language	English
Publishing date	2019-08-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Immunogenicity of standard and extended dosing intervals of BNT162b2 mRNA vaccine

Payne, Rebecca P. / Longet, Stephanie / Austin, James A. / Skelly, Donal T. / Dejnirattisai, Wanwisa / Adele, Sandra / Meardon, Naomi / Faustini, Sian / Al-Taei, Saly / Moore, Shona C. / Tipton, Tom / Hering, Luisa M. / Angyal, Adrienn / Brown, Rebecca / Nicols, Alexander R. / Gillson, Natalie / Dobson, Susan L. / Amini, Ali / Supasa, Piyada /

Cross, Andrew / Bridges-Webb, Alice / Reyes, Laura Silva / Linder, Aline / Sandhar, Gurjinder / Kilby, Jonathan A. / Tyerman, Jessica K. / Altmann, Thomas / Hornsby, Hailey / Whitham, Rachel / Phillips, Eloise / Malone, Tom / Hargreaves, Alexander / Shields, Adrian / Saei, Ayoub / Foulkes, Sarah / Stafford, Lizzie / Johnson, Sile / Wootton, Daniel G. / Conlon, Christopher P. / Jeffery, Katie / Matthews, Philippa C. / Frater, John / Deeks, Alexandra S. / Pollard, Andrew J. / Brown, Anthony / Rowland-Jones, Sarah L. / Mongkolsapaya, Juthathip / Barnes, Eleanor / Hopkins, Susan / Hall, Victoria / Dold, Christina / Duncan, Christopher J.A. / Richter, Alex / Carroll, Miles / Screaton, Gavin / de Silva, Thushan I. / Turtle, Lance / Klenerman, Paul / Dunachie, Susanna / Abuelgasim, Hibatullah / Adland, Emily / Adlou, Syed / Akther, Hossain Delowar / Alhussni, Ahmed / Ali, Mohammad / Ansari, M. Azim / Arancibia-Cárcamo, Carolina V. / Bayley, Martin / Brown, Helen / Chalk, Jeremy / Chand, Meera / Chawla, Anu / Chinnakannan, Senthil / Cutteridge, Jospeh / de Lara, Catherine / Denly, Lucy / Diffey, Ben / Dimitriadis, Stavros / Drake, Thomas M. / Donnison, Timothy / Dupont, Maeva / Eyre, David / Fairman, Alex / Gardiner, Siobhan / Gilbert-Jarmillo, Javier / Goulder, Philip / Hackstein, Carl-Philipp / Hambleton, Sophie / Haniffa, Muzlifah / Haworth, Jenny / Holmes, Jennifer / Horner, Emily / Jämsén, Anni / Jones, Chris / Kasanyinga, Mwila / Kelly, Sinead / Kirk, Rosemary / Knight, Michael L. / Lawrie, Allan / Lee, Lian / Lett, Lauren / Lillie, Katy / Lim, Nicholas / Mehta, Hema / Mentzer, Alexander J. / O’Donnell, Denise / Ogbe, Ane / Pace, Matthew / Payne, Brendan A.I. / Platt, Gareth / Poolan, Sonia / Provine, Nicholas / Ramamurthy, Narayan / Robinson, Nichola / Romaniuk, Leigh / Rongkard, Patpong / Sampson, Oliver L. / Simmons, Beatrice / Spegarova, Jarmila S. / Stephenson, Emily / Subramaniam, Kris / Thaventhiran, James / Thomas, Sarah / Travis, Simon / Tucker, Stephanie / Turton, Helena / Watson, Adam / Watson, Lisa / Weeks, Esme / Wilson, Robert / Wood, Steven / Wright, Rachel / Xiao, Huiyuan / Zawia, Amira A.T.

Cell. 2021 Nov. 11, v. 184, no. 23

2021

Abstract: Extension of the interval between vaccine doses for the BNT162b2 mRNA vaccine was introduced in the United Kingdom to accelerate population coverage with a single dose. At this time, trial data were lacking, and we addressed this in a study of United ... ...

Institution	on behalf of the PITCH Consortium
Abstract	Extension of the interval between vaccine doses for the BNT162b2 mRNA vaccine was introduced in the United Kingdom to accelerate population coverage with a single dose. At this time, trial data were lacking, and we addressed this in a study of United Kingdom healthcare workers. The first vaccine dose induced protection from infection from the circulating alpha (B.1.1.7) variant over several weeks. In a substudy of 589 individuals, we show that this single dose induces severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibody (NAb) responses and a sustained B and T cell response to the spike protein. NAb levels were higher after the extended dosing interval (6–14 weeks) compared with the conventional 3- to 4-week regimen, accompanied by enrichment of CD4⁺ T cells expressing interleukin-2 (IL-2). Prior SARS-CoV-2 infection amplified and accelerated the response. These data on dynamic cellular and humoral responses indicate that extension of the dosing interval is an effective immunogenic protocol.
Keywords	Severe acute respiratory syndrome coronavirus 2 ; health services ; immunogenicity ; interleukin-2 ; vaccines ; United Kingdom
Language	English
Dates of publication	2021-1111
Size	p. 5699-5714.e11.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	187009-9
ISSN	1097-4172 ; 0092-8674
ISSN (online)	1097-4172
ISSN	0092-8674
DOI	10.1016/j.cell.2021.10.011
Database	NAL-Catalogue (AGRICOLA)

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