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  1. Article ; Online: Assessment of Immunologically Potent Carbohydrates.

    Wang, Denong

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2612, Page(s) 129–139

    Abstract: Carbohydrates have a great potential in generating structural and immunological diversities. Microbial pathogens often decorate their outmost surfaces with specific carbohydrate signatures. Carbohydrate antigens also differ significantly from protein ... ...

    Abstract Carbohydrates have a great potential in generating structural and immunological diversities. Microbial pathogens often decorate their outmost surfaces with specific carbohydrate signatures. Carbohydrate antigens also differ significantly from protein antigens in physiochemical properties, especially in surface display of antigenic determinants in aqueous solutions. Technical optimization or modifications are often needed when we apply standard procedures for protein-based enzyme-linked immunosorbent assay (ELISA) to assess immunologically potent carbohydrates. We present here our laboratory protocols for performing carbohydrate ELISA and discuss several assay platforms that may be applied complementarily to explore the carbohydrate moieties that are critical for host immune recognition and induction of glycan-specific antibody responses.
    MeSH term(s) Carbohydrates/chemistry ; Polysaccharides/chemistry ; Antibodies/metabolism ; Antigens ; Proteins ; Enzyme-Linked Immunosorbent Assay
    Chemical Substances Carbohydrates ; Polysaccharides ; Antibodies ; Antigens ; Proteins
    Language English
    Publishing date 2023-02-14
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2903-1_10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Coronaviruses' sugar shields as vaccine candidates.

    Wang, Denong

    Current trends in immunology

    2020  Volume 21, Page(s) 17–23

    Abstract: A successful global healthcare response relies on versatile vaccines and production of broadly virus-neutralizing antibodies by the immune system to protect us from emerging infectious diseases. The present 2019 severe acute respiratory syndrome ... ...

    Abstract A successful global healthcare response relies on versatile vaccines and production of broadly virus-neutralizing antibodies by the immune system to protect us from emerging infectious diseases. The present 2019 severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic highlights the urgent need for development of anti-viral biodefense. Due to the genetic and proteomic diversities of viral pathogens, establishing versatile anti-viral vaccines or therapeutic agents is highly challenging. Carbohydrate antigens represent an important class of immunological targets for vaccine development and immunotherapy against microbial infections. In this mini review, some concepts and strategies for exploring the potential of immunogenic sugar moieties as CoV vaccine candidates are presented.
    Keywords covid19
    Language English
    Publishing date 2020-06-29
    Publishing country India
    Document type Journal Article
    ZDB-ID 2121311-2
    ISSN 0972-4567
    ISSN 0972-4567
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Se 2042: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Unraveling Sugar Chain Signatures of the "Seeds" of Tumor Metastasis.

    Wang, Denong

    Journal of proteomics & bioinformatics

    2017  Volume 10, Issue 1

    Language English
    Publishing date 2017-01-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2451945-5
    ISSN 0974-276X
    ISSN 0974-276X
    DOI 10.4172/jpb.1000e31
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  4. Article: The "Janus-like" RNA-editing machinery in innate antiviral immunity.

    Wu, Lisa / Wang, Denong

    Current trends in immunology

    2022  Volume 23, Page(s) 23–32

    Abstract: Our innate immune systems are evolved to provide the first line of immune defense against microbial infections. A key effector component is the adenosine deaminase acting on the RNA-1 (ADAR-1)/interferon (IFN) pathway of the innate cytoplasmic immunity ... ...

    Abstract Our innate immune systems are evolved to provide the first line of immune defense against microbial infections. A key effector component is the adenosine deaminase acting on the RNA-1 (ADAR-1)/interferon (IFN) pathway of the innate cytoplasmic immunity that mounts rapid responses to many viral pathogens. As an RNA-editing enzyme, ADAR-1 targets viral RNA intermediates in the cytoplasmic compartment to interfere with the infection. However, ADAR-1 may also edit characteristic RNA structures of certain host genes, notably, the 5-hydroxytryptamine (serotonin) receptor 2C (5-HT
    Language English
    Publishing date 2022-10-12
    Publishing country India
    Document type Journal Article
    ZDB-ID 2121311-2
    ISSN 0972-4567
    ISSN 0972-4567
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Se 2042: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Differential Expression of Blood Group Precursor Antigen in Human Breast Cancer Tissue.

    Jiang, Yi / Wang, Denong

    International journal of cancer science & therapy

    2020  Volume 2, Issue 1

    Abstract: There is a pressing need for biomarkers for targeted immunotherapy against breast cancer (BCA), the leading cause of cancer death in women. Previously, a blood group precursor O-core epitope ... ...

    Abstract There is a pressing need for biomarkers for targeted immunotherapy against breast cancer (BCA), the leading cause of cancer death in women. Previously, a blood group precursor O-core epitope gp
    Language English
    Publishing date 2020-08-14
    Publishing country United States
    Document type Journal Article
    DOI 10.31487/j.ijcst.2020.01.04
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  6. Article: Glyco-epitope Diversity: An Evolving Area of Glycomics Research and Biomarker Discovery.

    Wang, Denong

    Journal of proteomics & bioinformatics

    2014  Volume 7, Issue 2

    Keywords covid19
    Language English
    Publishing date 2014-06-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2451945-5
    ISSN 0974-276X
    ISSN 0974-276X
    DOI 10.4172/jpb.10000e24
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  7. Article ; Online: A Liposome-Based Approach to the Integrated Multi-Component Antigen Microarrays

    Denong Wang

    Microarrays, Vol 4, Iss 4, Pp 618-

    2015  Volume 629

    Abstract: This report describes an experimental procedure for constructing integrated lipid, carbohydrate, and protein microarrays. In essence, it prints liposomes on nitrocellulose-coated micro-glass slides, a biochip substrate for spotting protein and ... ...

    Abstract This report describes an experimental procedure for constructing integrated lipid, carbohydrate, and protein microarrays. In essence, it prints liposomes on nitrocellulose-coated micro-glass slides, a biochip substrate for spotting protein and carbohydrate microarrays, and the substances that can form liposomes (homo-liposomes) or can be incorporated into liposomes (hetero-liposomes) are suitable for microarray construction using existing microarray spotting devices. Importantly, this technology allows simultaneous detection of serum antibody activities among the three major classes of antigens, i.e., lipids, carbohydrates, and proteins. The potential of this technology is illustrated by its use in revealing a broad-spectrum of pre-existing anti-lipid antibodies in blood circulation and monitoring the epitope spreading of autoantibody reactivities among protein, carbohydrate, and lipid antigens in experimental autoimmune encephalomyelitis (EAE).
    Keywords lipids ; liposome ; liposome microarrays ; antigen microarrays ; natural antibodies ; autoantibodies ; high-throughput serology ; encephalomyelitis ; Analytical chemistry ; QD71-142 ; Chemistry ; QD1-999 ; Science ; Q
    Subject code 500
    Language English
    Publishing date 2015-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: A Liposome-Based Approach to the Integrated Multi-Component Antigen Microarrays.

    Wang, Denong

    Microarrays (Basel, Switzerland)

    2015  Volume 4, Issue 4, Page(s) 618–629

    Abstract: This report describes an experimental procedure for constructing integrated lipid, carbohydrate, and protein microarrays. In essence, it prints liposomes on nitrocellulose-coated micro-glass slides, a biochip substrate for spotting protein and ... ...

    Abstract This report describes an experimental procedure for constructing integrated lipid, carbohydrate, and protein microarrays. In essence, it prints liposomes on nitrocellulose-coated micro-glass slides, a biochip substrate for spotting protein and carbohydrate microarrays, and the substances that can form liposomes (homo-liposomes) or can be incorporated into liposomes (hetero-liposomes) are suitable for microarray construction using existing microarray spotting devices. Importantly, this technology allows simultaneous detection of serum antibody activities among the three major classes of antigens, i.e., lipids, carbohydrates, and proteins. The potential of this technology is illustrated by its use in revealing a broad-spectrum of pre-existing anti-lipid antibodies in blood circulation and monitoring the epitope spreading of autoantibody reactivities among protein, carbohydrate, and lipid antigens in experimental autoimmune encephalomyelitis (EAE).
    Language English
    Publishing date 2015-11-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662253-1
    ISSN 2076-3905
    ISSN 2076-3905
    DOI 10.3390/microarrays4040618
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  9. Article: N

    Wang, Denong

    Journal of proteomics & bioinformatics

    2014  Volume 5, Issue 4, Page(s) 90–95

    Abstract: Although tumor-associated abnormal glycosylation has been recognized for decades, information regarding host recognition of the evolving tumor glycome remains elusive. We report here a carbohydrate microarray analysis of a number of tumor-associated ... ...

    Abstract Although tumor-associated abnormal glycosylation has been recognized for decades, information regarding host recognition of the evolving tumor glycome remains elusive. We report here a carbohydrate microarray analysis of a number of tumor-associated carbohydrates for their serum antibody reactivities and potential immunogenicity in humans. These are the precursors, cores and internal sequences of
    Keywords covid19
    Language English
    Publishing date 2014-09-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2451945-5
    ISSN 0974-276X
    ISSN 0974-276X
    DOI 10.4172/jpb.1000218
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  10. Article: Liquid Biopsy in the OMICS Era of Tumor Medicine.

    Jiang, Yi / Wang, Denong

    Open access journal of biomedical engineering and its applications

    2018  Volume 1, Issue 3

    Abstract: Liquid biopsy uses noninvasive blood test to assess tumor heterogeneity and evolution in real time. It looks for tumor components in the blood circulation, such as circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA), to provide tumor- ... ...

    Abstract Liquid biopsy uses noninvasive blood test to assess tumor heterogeneity and evolution in real time. It looks for tumor components in the blood circulation, such as circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA), to provide tumor-specific information. By detecting multiplex tumor biomarkers, including nucleic acids, proteins, carbohydrates, and other tumor-derived substances, liquid biopsy helps with early tumor diagnosis, tumor evolution monitoring, and prognosis prediction. With the development of high-throughput OMICS tools like carbohydrate microarray and high-speed fiber-optic array scanning technology (FAST scan), it is now practical to identify glycan markers of CTCs and cancer stem cells (CSCs), especially those that are cell-surface exposed and readily accessible for immune recognition and targeting. Potential of this class of biomarkers in tumor subtyping and targeted immunotherapy is yet to be explored.
    Language English
    Publishing date 2018-03-07
    Publishing country United States
    Document type Journal Article
    Database MEDical Literature Analysis and Retrieval System OnLINE

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