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  1. Article: What about the Cytoskeletal and Related Proteins of Tapeworms in the Host's Immune Response? An Integrative Overview.

    Ríos-Valencia, Diana G / Ambrosio, Javier / Tirado-Mendoza, Rocío / Carrero, Julio César / Laclette, Juan Pedro

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 6

    Abstract: Recent advances have increased our understanding of the molecular machinery in the cytoskeleton of mammalian cells, in contrast to the case of tapeworm parasites, where cytoskeleton remains poorly characterized. The pertinence of a better knowledge of ... ...

    Abstract Recent advances have increased our understanding of the molecular machinery in the cytoskeleton of mammalian cells, in contrast to the case of tapeworm parasites, where cytoskeleton remains poorly characterized. The pertinence of a better knowledge of the tapeworm cytoskeleton is linked to the medical importance of these parasitic diseases in humans and animal stock. Moreover, its study could offer new possibilities for the development of more effective anti-parasitic drugs, as well as better strategies for their surveillance, prevention, and control. In the present review, we compile the results of recent experiments on the cytoskeleton of these parasites and analyze how these novel findings might trigger the development of new drugs or the redesign of those currently used in addition to supporting their use as biomarkers in cutting-edge diagnostic tests.
    Language English
    Publishing date 2023-06-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12060840
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  2. Article: Coupe de Grace: Autopsy in COVID-19 Pandemic.

    Rios-Valencia, Javier / Gamboa-Domínguez, Armando

    Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion

    2020  Volume 72, Issue 5

    Abstract: As the media informed the spread of a highly transmissible disease, our medical community faced the disappearance of a languishing ancient tool. Traditionally helping to explain unexplainable death, educate, audit, warn relatives, or contacts; autopsies ... ...

    Abstract As the media informed the spread of a highly transmissible disease, our medical community faced the disappearance of a languishing ancient tool. Traditionally helping to explain unexplainable death, educate, audit, warn relatives, or contacts; autopsies vanished in a reassigned COVID-19 teaching hospital (Fig. 1).
    Keywords covid19
    Language English
    Publishing date 2020-05-07
    Publishing country Mexico
    Document type Journal Article
    ZDB-ID 138348-6
    ISSN 0034-8376
    ISSN 0034-8376
    DOI 10.24875/RIC.20000262
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 288: Show issues Location:
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  3. Article ; Online: What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview

    Diana G. Ríos-Valencia / Javier Ambrosio / Rocío Tirado-Mendoza / Julio César Carrero / Juan Pedro Laclette

    Pathogens, Vol 12, Iss 840, p

    2023  Volume 840

    Abstract: Recent advances have increased our understanding of the molecular machinery in the cytoskeleton of mammalian cells, in contrast to the case of tapeworm parasites, where cytoskeleton remains poorly characterized. The pertinence of a better knowledge of ... ...

    Abstract Recent advances have increased our understanding of the molecular machinery in the cytoskeleton of mammalian cells, in contrast to the case of tapeworm parasites, where cytoskeleton remains poorly characterized. The pertinence of a better knowledge of the tapeworm cytoskeleton is linked to the medical importance of these parasitic diseases in humans and animal stock. Moreover, its study could offer new possibilities for the development of more effective anti-parasitic drugs, as well as better strategies for their surveillance, prevention, and control. In the present review, we compile the results of recent experiments on the cytoskeleton of these parasites and analyze how these novel findings might trigger the development of new drugs or the redesign of those currently used in addition to supporting their use as biomarkers in cutting-edge diagnostic tests.
    Keywords Cestoda ; tapeworm ; cytoskeleton ; diagnosis ; vaccine ; drug targets ; Medicine ; R
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Coupe de Grace: Autopsy in COVID-19 Pandemic

    Rios-Valencia, Javier / Gamboa-Domínguez, Armando

    Rev. invest. clin

    Abstract: As the media informed the spread of a highly transmissible disease, our medical community faced the disappearance of a languishing ancient tool. Traditionally helping to explain unexplainable death, educate, audit, warn relatives, or contacts; autopsies ... ...

    Abstract As the media informed the spread of a highly transmissible disease, our medical community faced the disappearance of a languishing ancient tool. Traditionally helping to explain unexplainable death, educate, audit, warn relatives, or contacts; autopsies vanished in a reassigned COVID-19 teaching hospital (Fig. 1).
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #869469
    Database COVID19

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  5. Article ; Online: Annexin in Taenia crassiceps ORF Strain is Localized in the Osmoregulatory System.

    Rios-Valencia, Diana G / Mompala-García, Yazmín / Marquez-Navarro, Adrián / Tirado-Mendoza, Rocío / Ambrosio, Javier

    Acta parasitologica

    2022  Volume 67, Issue 2, Page(s) 827–834

    Abstract: Purpose: Annexins are proteins with important roles in parasites, some of which are related to excretion-secretion processes, protein traffic, and microvesicle functionality. The participation of annexins in osmoregulation has been reported in tapeworms, ...

    Abstract Purpose: Annexins are proteins with important roles in parasites, some of which are related to excretion-secretion processes, protein traffic, and microvesicle functionality. The participation of annexins in osmoregulation has been reported in tapeworms, including Taenia solium. This study aimed to investigate the localization and expression of annexin in cysticerci of Taenia crassiceps, used as a model of cysticercosis.
    Methods: We used an antibody made with a protein, previously employed on Schistosoma bovis, to detect annexin in T. crassiceps proteins extracts used Western blot assay. The histological distribution of annexin was studied with immunofluorescence and confocal microscopy.
    Results: The antibody against annexin recognized a band at a molecular weight of 40.9 kDa. The histological distribution of annexin showed that the protein is mainly localized in the tegument and the protonephridia ducts.
    Conclusion: In our study, annexin was detected at a molecular weight similar to that described for Schistosoma bovis. In addition, its principal localization entailed structures of the osmoregulatory system one of the most important by the survival of the parasites. This confirms and solidifies previous reports concerning the role of annexins in T. crassiceps and this will be interesting by the development of new compounds against this protein.
    MeSH term(s) Animals ; Annexins/genetics ; Cysticercosis ; Cysticercus ; Mice ; Mice, Inbred BALB C ; Osmoregulation ; Parasites ; Taenia solium
    Chemical Substances Annexins
    Language English
    Publishing date 2022-02-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1132735-2
    ISSN 1896-1851 ; 0065-1478 ; 1230-2821
    ISSN (online) 1896-1851
    ISSN 0065-1478 ; 1230-2821
    DOI 10.1007/s11686-022-00526-4
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    In stock of ZB MED Cologne/Königswinter

    Uh II Zs.224: Show issues Location:
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  6. Article ; Online: Temperature-dependent secretion of Zika virus envelope and non-structural protein 1 in mammalian cells for clinical applications.

    Kim, Young Chan / Nettleship, Joanne E / García-Larragoiti, Nallely / Mar, Maria Antonieta / Mondragón-García, Ariadna Lorena / Ríos-Valencia, Javier / Figueroa-Aguilar, Gloria / Viveros-Sandoval, Martha Eva / Owens, Raymond J / Reyes-Sandoval, Arturo

    Journal of virological methods

    2021  Volume 294, Page(s) 114175

    Abstract: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus associated with congenital Zika syndrome and Guillain-Barré syndrome in adults. The recombinant ZIKV envelope (E) antigen can be useful for serodiagnosis of ZIKV infection and for monitoring ... ...

    Abstract Zika virus (ZIKV) is an emerging mosquito-borne flavivirus associated with congenital Zika syndrome and Guillain-Barré syndrome in adults. The recombinant ZIKV envelope (E) antigen can be useful for serodiagnosis of ZIKV infection and for monitoring immune responses during preclinical and clinical ZIKV vaccine development. In this study, we describe production of ZIKV E using the modified polyethyleneimine (PEI) transfection in HEK293 cells to improve cost-effective large-scale production. We show that the secretion of ZIKV E in HEK293 cells is dependent on cell culture incubation temperatures where incubation at a low temperature of 28 °C improved protein secretion of both, E-CD4 and E, whereas a substantial decrease in secretion was observed at 37 °C. The resulting E-CD4 produced at low temperature yielded similar binding profiles in ELISAs in comparison with a commercially available E protein using human seropositive sera to ZIKV. We also show that ZIKV NS1 and NS1 β-ladder antigens produced in HEK293 cells, have similar binding profiles in ELISA which suggests that both NS1 or NS1 β-ladder can be used for serodiagnosis of ZIKV. In conclusion, we propose a cost-effective production of the ZIKV E and NS1, suitable for both, clinical and research applications in endemic countries.
    MeSH term(s) Animals ; Antibodies, Viral ; HEK293 Cells ; Humans ; Temperature ; Viral Envelope ; Viral Nonstructural Proteins/genetics ; Zika Virus ; Zika Virus Infection/diagnosis
    Chemical Substances Antibodies, Viral ; Viral Nonstructural Proteins
    Language English
    Publishing date 2021-05-19
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2021.114175
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1565: Show issues Location:
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  7. Article: Temperature-dependent secretion of Zika virus envelope and non-structural protein 1 in mammalian cells for clinical applications

    Kim, Young Chan / Nettleship, Joanne E / García-Larragoiti, Nallely / Mar, Maria Antonieta / Mondragón-García, Ariadna Lorena / Ríos-Valencia, Javier / Figueroa-Aguilar, Gloria / Viveros-Sandoval, Martha Eva / Owens, Raymond J / Reyes-Sandoval, Arturo

    Journal of virological methods. 2021 Aug., v. 294

    2021  

    Abstract: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus associated with congenital Zika syndrome and Guillain-Barré syndrome in adults. The recombinant ZIKV envelope (E) antigen can be useful for serodiagnosis of ZIKV infection and for monitoring ... ...

    Abstract Zika virus (ZIKV) is an emerging mosquito-borne flavivirus associated with congenital Zika syndrome and Guillain-Barré syndrome in adults. The recombinant ZIKV envelope (E) antigen can be useful for serodiagnosis of ZIKV infection and for monitoring immune responses during preclinical and clinical ZIKV vaccine development. In this study, we describe production of ZIKV E using the modified polyethyleneimine (PEI) transfection in HEK293 cells to improve cost-effective large-scale production. We show that the secretion of ZIKV E in HEK293 cells is dependent on cell culture incubation temperatures where incubation at a low temperature of 28 °C improved protein secretion of both, E-CD4 and E, whereas a substantial decrease in secretion was observed at 37 °C. The resulting E-CD4 produced at low temperature yielded similar binding profiles in ELISAs in comparison with a commercially available E protein using human seropositive sera to ZIKV. We also show that ZIKV NS1 and NS1 β-ladder antigens produced in HEK293 cells, have similar binding profiles in ELISA which suggests that both NS1 or NS1 β-ladder can be used for serodiagnosis of ZIKV. In conclusion, we propose a cost-effective production of the ZIKV E and NS1, suitable for both, clinical and research applications in endemic countries.
    Keywords Zika virus ; antigens ; cell culture ; cost effectiveness ; humans ; polyethyleneimine ; protein secretion ; serodiagnosis ; seroprevalence ; temperature ; transfection ; vaccine development ; viral nonstructural proteins
    Language English
    Dates of publication 2021-08
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2021.114175
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1565: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Article: In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci

    Rios-Valencia, Diana G / López-Villegas, Edgar O / Diaz Chiguer, Dylan / Marquez Navarro, Adrian / Díaz-Martín, Ruben D / Nogueda-Torres, Benjamin / Ambrosio, Javier R

    Journal of parasitology research. 2019 Mar. 3, v. 2019

    2019  

    Abstract: Cytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. The aim of this work was ...

    Abstract Cytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. The aim of this work was to determine the presence of a septin-like protein in cysticerci of Taenia crassiceps and Taenia solium using the deduced amino acid sequence of T. solium septin 4 (SEPT4_Tsm), to design and synthesize a derived immunogenic peptide (residues 88 to 103), to prepare a specific rabbit polyclonal antibody, and to examine the effects of FCF at different concentrations and exposure times on an in vitro culture of T. crassiceps cysticerci. In vitro, FCF altered the morphology and motility of T. crassiceps cysticerci, and its effects were reversible under specific concentrations. In addition, we observed by ultrastructural observation that FCF alters the cellular subunit of the protonephridial system of cestodes, where disruption of the axoneme pattern of flame cells was observed. The rabbit polyclonal antibody prepared against the synthetic peptide recognized a major band of 41 kDa in both parasites. Our results establish the importance of SEPT4_Tsm in the dynamics and survival of taeniid cysticerci, as well as their susceptibility to FCF. This is also the first report that a septin is present in the cytoskeleton of taeniids.
    Keywords Taenia crassiceps ; Taenia solium ; cysticerci ; cytokinins ; cytoskeleton ; exposure duration ; forchlorfenuron ; genomics ; in vitro culture ; parasites ; polyclonal antibodies ; rabbits ; synthetic peptides
    Language English
    Dates of publication 2019-0303
    Publishing place Hindawi
    Document type Article
    ZDB-ID 2563542-6
    ISSN 2090-0031 ; 2090-0023
    ISSN (online) 2090-0031
    ISSN 2090-0023
    DOI 10.1155/2019/8578936
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  9. Article: In Vitro

    Rios-Valencia, Diana G / López-Villegas, Edgar O / Diaz Chiguer, Dylan / Marquez Navarro, Adrian / Díaz-Martín, Ruben D / Nogueda-Torres, Benjamin / Ambrosio, Javier R

    Journal of parasitology research

    2019  Volume 2019, Page(s) 8578936

    Abstract: Cytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. The aim of this work was ...

    Abstract Cytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. The aim of this work was to determine the presence of a septin-like protein in cysticerci of
    Language English
    Publishing date 2019-03-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2563542-6
    ISSN 2090-0031 ; 2090-0023
    ISSN (online) 2090-0031
    ISSN 2090-0023
    DOI 10.1155/2019/8578936
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  10. Article ; Online: An Ethical Imperative to Ensure Uninterrupted HIV Care Following Therapeutic Trials: One Experience in Peru.

    Garcia-Rosales, Katherine / Sosa Barbaran, Karin / Rios, Jessica / Pinto-Santini, Delia / Del Rosario Leon, Maria / Gallardo-Cartagena, Jorge A / MacRae, John / Leon, Mey / Valencia-Huamaní, Javier / Montalban, Esmelda / Gonzales, Pedro / Duerr, Ann / Lama, Javier R / Bender Ignacio, Rachel

    AIDS patient care and STDs

    2022  Volume 36, Issue 6, Page(s) 205–207

    MeSH term(s) HIV Infections/drug therapy ; HIV Infections/epidemiology ; Humans ; Peru/epidemiology
    Language English
    Publishing date 2022-06-10
    Publishing country United States
    Document type Letter
    ZDB-ID 1326868-5
    ISSN 1557-7449 ; 0893-5068 ; 1087-2914
    ISSN (online) 1557-7449
    ISSN 0893-5068 ; 1087-2914
    DOI 10.1089/apc.2022.0075
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2328: Show issues Location:
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