LIVIVO - Search results -

Search results

Result 1 - 10 of total 58546

Search options

Article ; Online: Re: Lui H, Galbraith JG, Meyers K, Bindra R, Lee SK. Biomechanical analysis of three techniques of suspensionplasty after trapeziectomy: a cadaveric study. J Hand Surg Eur. 2023, doi:10.1177/17531934231186495.

Ebner, Peggy / Kuschner, Stuart / Lui, Hayman / Galbraith, John G / Meyers, Kathleen / Bindra, Randy / Lee, Steve K

The Journal of hand surgery, European volume

2023 Volume 49, Issue 5, Page(s) 642–644

MeSH term(s)	Humans ; Trapezium Bone/surgery ; Biomechanical Phenomena ; Cadaver
Language	English
Publishing date	2023-12-06
Publishing country	England
Document type	Letter
ZDB-ID	2272801-6
ISSN	2043-6289 ; 1753-1934
ISSN (online)	2043-6289
ISSN	1753-1934
DOI	10.1177/17531934231217371
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1940: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Performance Evaluation of the KRYPTOR Compact PLUS Analyzer-Based B.R.A.H.M.S. CgA Ⅱ KRYPTOR Assay for Chromogranin A Measurement.

Choi, Yu Jeong / Roh, Juhye / Kim, Sinyoung / Lee, Kyung-A / Park, Younhee

Diagnostics (Basel, Switzerland)

2021 Volume 11, Issue 12

Abstract: Numerous immunoassays have been developed to measure the levels of chromogranin A (CgA), a useful biomarker for diagnosing and monitoring generally heterogeneous neuroendocrine tumors (NETs). Here, we evaluated the imprecision and linearity of three such ...

Abstract	Numerous immunoassays have been developed to measure the levels of chromogranin A (CgA), a useful biomarker for diagnosing and monitoring generally heterogeneous neuroendocrine tumors (NETs). Here, we evaluated the imprecision and linearity of three such assays: KRYPTOR (ThermoFisher Scientific), NEOLISA (EuroDiagnostica), and CgA-RIA (CisBio), using 123 samples for each assay. The correlation coefficients between the assays were 0.932 (CgA-RIA versus NEOLISA), 0.956 (KRYPTOR versus CgA-RIA), and 0.873 (NEOLISA versus KRYPTOR). KRYPTOR showed good precision, with percent coefficients of variation less than 5% for low and high concentration quality controls. Linearity was maintained over a wide concentration range. Comparison of CgA levels from three disease entities (NETs, non-NET pancreatic tumors, and prostate cancer) and healthy controls showed that patients with NETs had significantly higher CgA levels (
Language	English
Publishing date	2021-12-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics11122400
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Discovery of ( R)-8-(6-Methyl-4-oxo-1,4,5,6-tetrahydropyrrolo[3,4- b]pyrrol-2-yl)-3-(1-methylcyclopropyl)-2-((1-methylcyclopropyl)amino)quinazolin-4(3 H)-one, a Potent and Selective Pim-1/2 Kinase Inhibitor for Hematological Malignancies.

Wang, Hui-Ling / Andrews, Kristin L / Booker, Shon K / Canon, Jude / Cee, Victor J / Chavez, Frank / Chen, Yuping / Eastwood, Heather / Guerrero, Nadia / Herberich, Brad / Hickman, Dean / Lanman, Brian A / Laszlo, Jimmy / Lee, Matthew R / Lipford, J Russell / Mattson, Bethany / Mohr, Christopher / Nguyen, Yen / Norman, Mark H /

Pettus, Liping H / Powers, David / Reed, Anthony B / Rex, Karen / Sastri, Christine / Tamayo, Nuria / Wang, Paul / Winston, Jeffrey T / Wu, Bin / Wu, Qiong / Wu, Tian / Wurz, Ryan P / Xu, Yang / Zhou, Yihong / Tasker, Andrew S

Journal of medicinal chemistry

2019 Volume 62, Issue 3, Page(s) 1523–1540

Abstract: ... with significant inhibition of BAD phosphorylation in KMS-12-BM multiple myeloma tumors for 16 h postdose. In a 2 ...

Abstract	Pim kinases are a family of constitutively active serine/threonine kinases that are partially redundant and regulate multiple pathways important for cell growth and survival. In human disease, high expression of the three Pim isoforms has been implicated in the progression of hematopoietic and solid tumor cancers, which suggests that Pim kinase inhibitors could provide patients with therapeutic benefit. Herein, we describe the structure-guided optimization of a series of quinazolinone-pyrrolodihydropyrrolone analogs leading to the identification of potent pan-Pim inhibitor 28 with improved potency, solubility, and drug-like properties. Compound 28 demonstrated on-target Pim activity in an in vivo pharmacodynamic assay with significant inhibition of BAD phosphorylation in KMS-12-BM multiple myeloma tumors for 16 h postdose. In a 2-week mouse xenograft model, daily dosing of compound 28 resulted in 33% tumor regression at 100 mg/kg.
MeSH term(s)	Animals ; Antineoplastic Agents/chemical synthesis ; Antineoplastic Agents/pharmacokinetics ; Antineoplastic Agents/therapeutic use ; Female ; Hematologic Neoplasms/drug therapy ; Humans ; Mice, SCID ; Molecular Structure ; Protein Kinase Inhibitors/chemical synthesis ; Protein Kinase Inhibitors/pharmacokinetics ; Protein Kinase Inhibitors/therapeutic use ; Proto-Oncogene Proteins c-pim-1/antagonists & inhibitors ; Pyrroles/chemical synthesis ; Pyrroles/pharmacokinetics ; Pyrroles/therapeutic use ; Quinazolinones/chemical synthesis ; Quinazolinones/pharmacokinetics ; Quinazolinones/therapeutic use ; Structure-Activity Relationship ; Swine ; Xenograft Model Antitumor Assays
Chemical Substances	Antineoplastic Agents ; Protein Kinase Inhibitors ; Pyrroles ; Quinazolinones ; Proto-Oncogene Proteins c-pim-1 (EC 2.7.11.1)
Language	English
Publishing date	2019-01-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	218133-2
ISSN	1520-4804 ; 0022-2623
ISSN (online)	1520-4804
ISSN	0022-2623
DOI	10.1021/acs.jmedchem.8b01733
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 151: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MB 1: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Performance Evaluation of the KRYPTOR Compact PLUS Analyzer-Based B.R.A.H.M.S. CgA Ⅱ KRYPTOR Assay for Chromogranin A Measurement

Yu Jeong Choi / Juhye Roh / Sinyoung Kim / Kyung-A Lee / Younhee Park

Diagnostics, Vol 11, Iss 2400, p

2021 Volume 2400

Abstract	Numerous immunoassays have been developed to measure the levels of chromogranin A (CgA), a useful biomarker for diagnosing and monitoring generally heterogeneous neuroendocrine tumors (NETs). Here, we evaluated the imprecision and linearity of three such assays: KRYPTOR (ThermoFisher Scientific), NEOLISA (EuroDiagnostica), and CgA-RIA (CisBio), using 123 samples for each assay. The correlation coefficients between the assays were 0.932 (CgA-RIA versus NEOLISA), 0.956 (KRYPTOR versus CgA-RIA), and 0.873 (NEOLISA versus KRYPTOR). KRYPTOR showed good precision, with percent coefficients of variation less than 5% for low and high concentration quality controls. Linearity was maintained over a wide concentration range. Comparison of CgA levels from three disease entities (NETs, non-NET pancreatic tumors, and prostate cancer) and healthy controls showed that patients with NETs had significantly higher CgA levels ( n = 57, mean: 1.82 ± 0.43 log ng/mL) than healthy individuals ( n = 20, mean: 1.51 ± 0.23 log ng/mL; p = 0.018). No other significant differences between groups were observed. All three immunoassays showed strong correlations in measured CgA levels. Because KRYPTOR operation uses a fully automated random-access system and requires shorter incubation times and smaller sample volumes, the KRYPTOR assay may improve laboratory workflow while maintaining satisfactory analytical performance.
Keywords	chromogranin A ; neuroendocrine tumor ; biomarker ; Medicine (General) ; R5-920
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Discovery of ( S)-3-(3-(3,5-Dimethyl-1 H-pyrazol-1-yl)phenyl)-4-(( R)-3-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)pyrrolidin-1-yl)butanoic Acid, a Nonpeptidic α

Journal of medicinal chemistry

2018 Volume 61, Issue 18, Page(s) 8417–8443

Abstract: ... via a rhodium catalyzed asymmetric 1,4-addition of arylboronic acids in the presence of ( R)-BINAP ...

Abstract	A series of 3-aryl(pyrrolidin-1-yl)butanoic acids were synthesized using a diastereoselective route, via a rhodium catalyzed asymmetric 1,4-addition of arylboronic acids in the presence of ( R)-BINAP to a crotonate ester to provide the ( S) absolute configuration for the major product. A variety of aryl substituents including morpholine, pyrazole, triazole, imidazole, and cyclic ether were screened in cell adhesion assays for affinity against α
MeSH term(s)	Animals ; Antigens, Neoplasm ; Cell Adhesion ; Dogs ; Drug Discovery ; Humans ; Idiopathic Pulmonary Fibrosis/drug therapy ; Integrins/antagonists & inhibitors ; Lung/drug effects ; Lung/metabolism ; Male ; Mice ; Models, Molecular ; Molecular Structure ; Protein Conformation ; Pyrazoles/chemistry ; Rats ; Rats, Wistar ; Structure-Activity Relationship ; Tissue Distribution
Chemical Substances	Antigens, Neoplasm ; Integrins ; Pyrazoles ; integrin alphavbeta6
Language	English
Publishing date	2018-09-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218133-2
ISSN	1520-4804 ; 0022-2623
ISSN (online)	1520-4804
ISSN	0022-2623
DOI	10.1021/acs.jmedchem.8b00959
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 151: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MB 1: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The complete mitochondrial genome of an Antarctic moss Syntrichia filaris (Müll.Hal.) R.H. Zander.

Yoon, Young-Jun / Kang, Yoonjee / Kim, Mi-Kyeong / Lee, Jungeun / Park, Hyun / Kim, Ji Hee / Lee, Hyoungseok

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis

2016 Volume 27, Issue 4, Page(s) 2779–2780

Abstract: The mitogenome of the Syntrichia filaris (GenBank accession number KP984758) has a total length of 106,343 bp and consists of 40 protein-coding genes, 3 ribosomal RNA (rRNA) and 24 transfer RNA. The mitochondrial structure and gene order was similar to ... ...

Abstract	The mitogenome of the Syntrichia filaris (GenBank accession number KP984758) has a total length of 106,343 bp and consists of 40 protein-coding genes, 3 ribosomal RNA (rRNA) and 24 transfer RNA. The mitochondrial structure and gene order was similar to other Bryophytes. Phylogenetic tree based on the combined analysis of amino acid sequences of 31 mitochondrial genes common in S. filaris, 17 Bryophyta and 3 Marchantiophyta, was well congruent with traditional species relationship of the moss order Pottiales.
Language	English
Publishing date	2016-07
Publishing country	England
Document type	Journal Article
ISSN	2470-1408
ISSN (online)	2470-1408
DOI	10.3109/19401736.2015.1053062
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Emergence of novel and dominant acquired EGFR solvent-front mutations at Gly796 (G796S/R) together with C797S/R and L792F/H mutations in one EGFR (L858R/T790M) NSCLC patient who progressed on osimertinib.

Ou, Sai-Hong Ignatius / Cui, Jean / Schrock, Alexa B / Goldberg, Michael E / Zhu, Viola W / Albacker, Lee / Stephens, Philip J / Miller, Vincent A / Ali, Siraj M

Lung cancer (Amsterdam, Netherlands)

2017 Volume 108, Page(s) 228–231

Abstract: ... the emergence of novel EGFR solvent front mutations at Gly796 (G796S/R) in addition to a hinge pocket L792F/H ... with each other including distinct mutations at the same codon producing different amino acid changes: G796S/R (mutant ... allele frequency [MAF]; 14.4%), C797S/G (MAF: 2.26%), L792F/H (MAF: 0.36%), and V802F (MAF: 0.40%), in addition ...

Abstract	Acquired epidermal growth factor receptor (EGFR) resistance mutations to osimertinib are common, including the EGFR C797S that abolishes the covalent binding of osimertinib to EGFR. Here we report the emergence of novel EGFR solvent front mutations at Gly796 (G796S/R) in addition to a hinge pocket L792F/H mutations, and C797S/G all in cis with T790M in a single patient on progression on osimertinib as detected by plasma circulating tumor DNA (ctDNA) assay in the course of clinical care. A 69-year-old Caucasian female former light-smoker presented with stage IV EGFR L858R positive adenocarcinoma who developed EGFR T790M mutation after 8 month treatment of erlotinib. The patient was initiated on osimertinib with disease shrinkage after 2 months, but tumor regrowth was observed after 5 months of osimertinib treatment. Assay of plasma ctDNA at this time revealed these different secondary resistance mutations all in trans with each other including distinct mutations at the same codon producing different amino acid changes: G796S/R (mutant allele frequency [MAF]; 14.4%), C797S/G (MAF: 2.26%), L792F/H (MAF: 0.36%), and V802F (MAF: 0.40%), in addition to the pre-existing L858R (MAF:17.9%) and T790M (MAF:18.2%) but all in cis with T790M. The G796S/R mutations are homologous with known reported solvent front mutations in ALK G1202R, ROS1 G2032R, TrkA G595R and TrkC G623R, all of which are associated with acquired resistance to type I TKIs. In silico modeling revealed mutation at G796 interferes with osimertinib binding to the EGFR kinase domain at the phenyl aromatic ring position as this residue forms a narrow "hydrophobic sandwich" with L718, while L792F/H mutation interferes with osimertinib binding at the methoxyl group on the phenyl ring. Multiple resistance mutations at differing allele frequencies including novel EGFR solvent front mutations can emerge in a single patient with progression on osimertinib potentially due to tumor hetereogeneity and definitely present a significant therapeutic and drug development challenge.
MeSH term(s)	Acrylamides ; Aged ; Alleles ; Amino Acid Substitution ; Aniline Compounds ; Animals ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/therapeutic use ; Carcinoma, Non-Small-Cell Lung/diagnosis ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Carcinoma, Non-Small-Cell Lung/genetics ; Disease Models, Animal ; ErbB Receptors/chemistry ; ErbB Receptors/genetics ; Female ; Humans ; Lung Neoplasms/diagnosis ; Lung Neoplasms/drug therapy ; Lung Neoplasms/genetics ; Models, Molecular ; Molecular Conformation ; Mutation ; Neoplasm Staging ; Piperazines/chemistry ; Piperazines/therapeutic use ; Protein Binding ; Protein Kinase Inhibitors/chemistry ; Protein Kinase Inhibitors/therapeutic use
Chemical Substances	Acrylamides ; Aniline Compounds ; Antineoplastic Agents ; Piperazines ; Protein Kinase Inhibitors ; osimertinib (3C06JJ0Z2O) ; ErbB Receptors (EC 2.7.10.1)
Language	English
Publishing date	2017-04-12
Publishing country	Ireland
Document type	Case Reports ; Journal Article
ZDB-ID	632771-0
ISSN	1872-8332 ; 0169-5002
ISSN (online)	1872-8332
ISSN	0169-5002
DOI	10.1016/j.lungcan.2017.04.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2135: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 423: Show issues

Order via subito

Details ▾

Article ; Online: A Fragment-Derived Clinical Candidate for Antagonism of X-Linked and Cellular Inhibitor of Apoptosis Proteins: 1-(6-[(4-Fluorophenyl)methyl]-5-(hydroxymethyl)-3,3-dimethyl-1 H,2 H,3 H-pyrrolo[3,2- b]pyridin-1-yl)-2-[(2 R,5 R)-5-methyl-2-([(3R)-3-methylmorpholin-4-yl]methyl)piperazin-1-yl]ethan-1-one (ASTX660).

Journal of medicinal chemistry

2018 Volume 61, Issue 16, Page(s) 7314–7329

Abstract: Inhibitor of apoptosis proteins (IAPs) are promising anticancer targets, given their roles in the evasion of apoptosis. Several peptidomimetic IAP antagonists, with inherent selectivity for cellular IAP (cIAP) over X-linked IAP (XIAP), have been tested ... ...

Abstract	Inhibitor of apoptosis proteins (IAPs) are promising anticancer targets, given their roles in the evasion of apoptosis. Several peptidomimetic IAP antagonists, with inherent selectivity for cellular IAP (cIAP) over X-linked IAP (XIAP), have been tested in the clinic. A fragment screening approach followed by structure-based optimization has previously been reported that resulted in a low-nanomolar cIAP1 and XIAP antagonist lead molecule with a more balanced cIAP-XIAP profile. We now report the further structure-guided optimization of the lead, with a view to improving the metabolic stability and cardiac safety profile, to give the nonpeptidomimetic antagonist clinical candidate 27 (ASTX660), currently being tested in a phase 1/2 clinical trial (NCT02503423).
MeSH term(s)	Administration, Oral ; Animals ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacokinetics ; Antineoplastic Agents/pharmacology ; Cell Line, Tumor ; Crystallography, X-Ray ; ERG1 Potassium Channel/antagonists & inhibitors ; Heterocyclic Compounds, 2-Ring/chemistry ; Heterocyclic Compounds, 2-Ring/pharmacokinetics ; Heterocyclic Compounds, 2-Ring/pharmacology ; Humans ; Inhibitor of Apoptosis Proteins/antagonists & inhibitors ; Macaca fascicularis ; Male ; Mice, Inbred BALB C ; Piperazines/chemistry ; Piperazines/pharmacokinetics ; Piperazines/pharmacology ; Rats, Sprague-Dawley ; Structure-Activity Relationship ; X-Linked Inhibitor of Apoptosis Protein/antagonists & inhibitors ; X-Linked Inhibitor of Apoptosis Protein/chemistry ; X-Linked Inhibitor of Apoptosis Protein/metabolism ; Xenograft Model Antitumor Assays
Chemical Substances	AT-IAP compound ; Antineoplastic Agents ; ERG1 Potassium Channel ; Heterocyclic Compounds, 2-Ring ; Inhibitor of Apoptosis Proteins ; KCNH2 protein, human ; Piperazines ; X-Linked Inhibitor of Apoptosis Protein ; XIAP protein, human
Language	English
Publishing date	2018-08-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	218133-2
ISSN	1520-4804 ; 0022-2623
ISSN (online)	1520-4804
ISSN	0022-2623
DOI	10.1021/acs.jmedchem.8b00900
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 151: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MB 1: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Erratum to "Emergence of novel and dominant acquired EGFR solvent-front mutations at Gly796 (G796S/R) together with C797S/G and L792F/H mutations in one EGFR (L858R/T790M) NSCLC patient who progressed on osimertinib" [Lung Cancer, 108 (June 2017) 228-231].

Ou, Sai-Hong Ignatius / Cui, Jean / Schrock, Alexa B / Goldberg, Michael E / Zhu, Viola W / Albacker, Lee / Stephens, Philip J / Miller, Vincent A / Ali, Siraj M

Lung cancer (Amsterdam, Netherlands)

2019 Volume 138, Page(s) 141

Language	English
Publishing date	2019-08-22
Publishing country	Ireland
Document type	Published Erratum
ZDB-ID	632771-0
ISSN	1872-8332 ; 0169-5002
ISSN (online)	1872-8332
ISSN	0169-5002
DOI	10.1016/j.lungcan.2019.08.013
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2135: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 423: Show issues

Order via subito

Details ▾

Article ; Online: Rapid Authentication of the Herbal Medicine Plant Species Aralia continentalis Kitag. and Angelica biserrata C.Q. Yuan and R.H. Shan Using ITS2 Sequences and Multiplex-SCAR Markers.

Kim, Wook Jin / Moon, Byeong Cheol / Yang, Sungyu / Han, Kyeong Suk / Choi, Goya / Lee, A Yeong

Molecules (Basel, Switzerland)

2016 Volume 21, Issue 3, Page(s) 270

Abstract: Accurate identification of the plant species that are present in herbal medicines is important for quality control. Although the dried roots of Aralia continentalis (Araliae Continentalis Radix) and Angelica biserrata (Angelicae Pubescentis Radix) are ... ...

Abstract	Accurate identification of the plant species that are present in herbal medicines is important for quality control. Although the dried roots of Aralia continentalis (Araliae Continentalis Radix) and Angelica biserrata (Angelicae Pubescentis Radix) are used in the same traditional medicine, namely Dok-Hwal in Korean and Du-Huo in Chinese, the medicines are described differently in the national pharmacopeia. Further confusion arises from the distribution of dried Levisticum officinale and Heracleum moellendorffii roots as the same medicine. Medicinal ingredients from all four plants are morphologically similar, and discrimination is difficult using conventional methods. Molecular identification methods offer rapidity and accuracy. The internal transcribed spacer 2 (ITS2) region of the nuclear ribosomal RNA gene (rDNA) was sequenced in all four plant species, and the sequences were used to design species-specific primers. Primers for each species were then combined to allow sample analysis in a single PCR reaction. Commercial herbal medicine samples were obtained from Korea and China and analyzed using the multiplex assay. The assay successfully identified authentic medicines and also identified inauthentic or adulterated samples. The multiplex assay will be a useful tool for identification of authentic Araliae Continentalis Radix and/or Angelicae Pubescentis Radix preparations in Korea and China.
MeSH term(s)	Angelica/classification ; Angelica/genetics ; Aralia/classification ; Aralia/genetics ; DNA Fingerprinting/methods ; DNA Primers/genetics ; DNA, Plant/analysis ; DNA, Ribosomal Spacer/analysis ; Genetic Markers/genetics ; Multiplex Polymerase Chain Reaction/methods ; Phylogeny ; Plants, Medicinal/classification ; Plants, Medicinal/genetics ; Sequence Analysis, DNA ; Species Specificity
Chemical Substances	DNA Primers ; DNA, Plant ; DNA, Ribosomal Spacer ; Genetic Markers
Language	English
Publishing date	2016-02-29
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules21030270
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.MO 81: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito