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Article ; Online: Genome-wide 5'-C-phosphate-G-3' methylation patterns reveal the effect of heat stress on the altered semen quality in Bubalus bubalis.

Kumar, Gautam / Gurao, Ankita / Vasisth, Rashi / Chitkara, Meenakshi / Singh, Ravinder / Ranganatha Sriranga, Karpenahalli / Shivanand Dige, Mahesh / Mukesh, Manishi / Singh, Pawan / Singh Kataria, Ranjit

Gene

2024 Volume 906, Page(s) 148233

Abstract: Semen production and quality are closely correlated with different environmental factors in bovines, particularly for the buffalo (Bubalus bubalis) bulls reared under tropical and sub-tropical conditions. Factors including DNA methylation patterns, an ... ...

Abstract	Semen production and quality are closely correlated with different environmental factors in bovines, particularly for the buffalo (Bubalus bubalis) bulls reared under tropical and sub-tropical conditions. Factors including DNA methylation patterns, an intricate process in sperm cells, have an impact on the production of quality semen in buffalo bulls under abiotic stress conditions. The present study was conducted to identify DNA methylome signatures for semen quality in Murrah buffalo bulls, acclaimed as a major dairy breed globally, under summer heat stress. Based on semen quality parameters that significantly varied between the two groups over the seasons, the breeding bulls were classified into seasonally affected (SA = 6) and seasonally non-affected (SNA = 6) categories. DNA was isolated from purified sperm cells and sequenced using the RRBS (Reduced Representation Bisulfite Sequencing) technique for genome-wide methylome data generation. During the hot summer months, the physiological parameters such as scrotal surface temperature, rectal temperature, and respiration rate for both the SA and SNA bulls were significantly higher in the afternoon than in the morning. Whereas, the global CpG% of SA bulls was positively correlated with the afternoon's scrotal surface and rectal temperature. The RRBS results conveyed differentially methylated cytosines in the promoter region of the genes encoding the channels responsible for Ca
MeSH term(s)	Animals ; Male ; Cattle/genetics ; Semen Analysis ; Semen/physiology ; Buffaloes/genetics ; Phosphates ; Spermatozoa ; DNA Methylation ; Heat-Shock Response/genetics ; Sperm Motility
Chemical Substances	Phosphates
Language	English
Publishing date	2024-02-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2024.148233
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Article: Hydrophilic porous asymmetric ultrafiltration membranes of aramid-g-PEO copolymers

Molina, Serena / Ãngel E. Lozano / Javier de Abajo / JosÃ© G. de la Campa / Paula Carretero / Shivanand B. Teli

Journal of Membrane Science. 2014 Mar. 15, v. 454

2014

Abstract: A series of experimental aramid-g-PEO copolymers was employed to fabricate ultrafiltration (UF ... In fouling tests performed using a bovine serum albumin (BSA) solution, aramid-g-PEO UF membranes exhibited ... to polyamide UF membranes. The results of this work indicate that aramid-g-PEO copolymers are promising ...

Abstract	A series of experimental aramid-g-PEO copolymers was employed to fabricate ultrafiltration (UF) membranes by the conventional method of phase inversion, using N,N-dimethylformamide as the solvent and water as the coagulating medium. By adjusting the dope concentration for each polymer, porous membranes were attained which were suitable for UF operations and were tested using a laboratory-scale cross-flow test unit. The water flux of the membranes showed a strong dependency on the chemical composition, with water permeability increasing with increasing PEO content in the copolymer. Their separation potentials were investigated using standard solutions of poly(ethylene oxide) (PEO) as the feed, and the performance of the membranes in UF operations was systematically compared. A relationship between the dope concentration and the molecular weight cut-off (MWCO) could be observed, with higher concentrations resulting in a lower MWCO. In contrast, it was observed that the higher the content of PEO in the copolymer the greater the MWCO. In fouling tests performed using a bovine serum albumin (BSA) solution, aramid-g-PEO UF membranes exhibited very good antifouling properties compared to a commercially sourced polysulfone membrane and to polyamide UF membranes. The results of this work indicate that aramid-g-PEO copolymers are promising materials for the fabrication of fouling resistant membranes for biomacromolecules' separations.
Keywords	artificial membranes ; bovine serum albumin ; chemical composition ; composite polymers ; ethylene oxide ; fouling ; hydrophilicity ; molecular weight ; permeability ; polyamides ; polyethylene glycol ; solvents ; ultrafiltration
Language	English
Dates of publication	2014-0315
Size	p. 233-242.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	194516-6
ISSN	0376-7388
ISSN	0376-7388
DOI	10.1016/j.memsci.2013.11.025
Database	NAL-Catalogue (AGRICOLA)

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Article: In Memoriam: Warren G. Sanger, PhD (October 6, 1945-February 5, 2015).

Sanmann, Jennifer N / McGavran, Loris / Patil, Shivanand R

Cancer genetics

2015 Volume 208, Issue 4, Page(s) 105–106

MeSH term(s)	Cytogenetics/history ; History, 20th Century ; History, 21st Century ; Humans ; United States
Language	English
Publishing date	2015-06-30
Publishing country	United States
Document type	Biography ; Historical Article ; Journal Article ; Portrait
ZDB-ID	2599227-2
ISSN	2210-7762
ISSN	2210-7762
DOI	10.1016/j.cancergen.2015.03.002
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Article ; Online: A COMPARATIVE STUDY TO KNOW THE INCIDENCE OF POST-DURAL PUNCTURE HEADACHE FOLLOWING SUBARACHNOID BLOCK USING 27 G QUINCKE’S AND WHITACRE NEEDLES - A RANDOMIZED CLINICAL TRIAL

Shivanand Laxman / Basvaraj

Journal of Evolution of Medical and Dental Sciences, Vol 5, Iss 49, Pp 3136-

2016 Volume 3139

Abstract: ... this difference was statistically significant (p<0.005). CONCLUSION Overall, the Whitacre 27 G needle has better ...

Abstract	BACKGROUND The introduction of local anaesthetic solutions into subarachnoid space produces Spinal Anaesthesia (SA). Spinal anaesthesia can be used either alternative to the general anaesthesia or can be used simultaneously with general anaesthesia or afterwards for postoperative analgesia and for the management of acute and chronic pain disorder. OBJECTIVES The study is carried out to evaluate the following parameters on using 27G Quincke’s and Whitacre’s spinal needles and to know the incidence of post dural puncture headache. METHODOLOGY The present study was conducted in the Department of Anaesthesiology, BLDEU, Shri B.M. Patil Medical College, Vijaypur, from January 2015 to December 2015. Patients between the age of 20 to 60 years with ASA grade I and II undergoing lower abdominal and lower limb surgeries were included. RESULTS Females outnumbered males in both the groups (56.1% and 57.2%) with male-to-female ratio of 1:1.31 in Quincke group and 1:1.37 in Whitacre group. Majority (30.13%) of the patients had age between 20 to 29 years in Quincke group compared to 30.12% of the patients in the age ranging from 50 to 60 years in Whitacre group. The incidence of PDPH is 3.1% in Quincke group and 1.1% patients in Whitracre group and this difference was statistically significant (p<0.005). CONCLUSION Overall, the Whitacre 27 G needle has better results with respect to PDPH.
Keywords	Post Dural Puncture Headache; Sub-Arachnoid Block; Quincke Needle; Whitacre Needle. ; Dentistry ; RK1-715 ; Medicine ; R ; Medicine (General) ; R5-920
Subject code	610
Language	English
Publishing date	2016-06-01T00:00:00Z
Publisher	Akshantala Enterprises
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Experimental and theoretical investigation of Low-Frequency vibrational modes of 4-Amino 3,5 Dinitro Pyrazole in terahertz frequency domain.

Koalla, Rajesh / Mukherjee, Supratik / Mangali, Shivanand / Vaitheeswaran, G / Kumar Chaudhary, Anil

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2024 Volume 313, Page(s) 124092

Abstract: Pyrazoles have recently received significant attention due to their unique and potential applications in the medical field, agriculture and are also known to be highly stable explosives. The present work describes the terahertz time-domain spectroscopy ( ... ...

Abstract	Pyrazoles have recently received significant attention due to their unique and potential applications in the medical field, agriculture and are also known to be highly stable explosives. The present work describes the terahertz time-domain spectroscopy (THz-TDS) based study of 4-Amino 3,5 Dinitro Pyrazole(ADNP) in between the 0.1 and 3.0 THz ranges. A Toptica-Teraflash fibre-coupled handheld terahertz system has been employed in reflection mode configuration. We ascertained complex refractive index, absorption coefficients, and complex dielectric constants from 0.1 THz to 3.0 THz. The value of the refractive index and absorption coefficients are found to be 1.8 and 10---180 cm
Language	English
Publishing date	2024-02-28
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2024.124092
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Article ; Online: A Randomized Comparative Study between Dexmedetomidine and Fentanyl on Attenuating Stress Response and Airway Response to Tracheal Extubation

Nikhila T / D. G. Talikoti / Shivanand L. K

Journal of Krishna Institute of Medical Sciences University, Vol 10, Iss 01, Pp 84-

2021 Volume 92

Abstract: Background: Tracheal extubation and emergence is associated with major hemodynamic alterations and is poorly tolerated by patients of comorbid disorders. The efficacy of dexmedetomidine and fentanyl was contrasted in mitigating hemodynamic stress ... ...

Abstract	Background: Tracheal extubation and emergence is associated with major hemodynamic alterations and is poorly tolerated by patients of comorbid disorders. The efficacy of dexmedetomidine and fentanyl was contrasted in mitigating hemodynamic stress response in study groups and evaluated the quality of extubation. Aim and Objectives: To study the efficacy of dexmedetomidine and fentanyl on the attenuation of hemodynamic responses and airway reflexes during extubation following surgery under general anaesthesia. Material and Methods: A randomized comparative study was conducted in Shri B. M. Patil Medical College Hospital and Research Centre, Vijayapura. Total of 60 patients scheduled for various surgical procedures under general anaesthesia were allotted into two groups. Group D: Intravenous dexmedetomidine 0.4mcg/kg body weight. Group F: Intravenous fentanyl 0.5 mcg/kg body weight. Results: Peak rise in mean heart rate was 86.7 % in Group D and 117.5% in Group F, Peak rise in systolic blood pressure was 150.3% in Group F and 109.8% in Group D, peak rise in mean arterial blood pressure was 115.7% in Group F and 88.2% in Group D and diastolic blood pressure rise was 98.9% in Group F and 78.2% in Group D respectively. Dexmedetomidine group had better extubation quality than the fentanyl group. Conclusion: Intravenous injection of dexmedetomidine administered 15 minutes before tracheal extubation was better as compared to intravenous injection of fentanyl in attenuating airway and hemodynamic reflexes to a greater extent allowing smooth and easy tracheal extubation, thus providing comfortable recovery.
Keywords	dexmedetomidine ; fentanyl ; tracheal extubation ; hemodynamic response ; Medicine ; R ; Medicine (General) ; R5-920
Subject code	796 ; 610
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Krishna Institute of Medical Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Short Communication

DE-HWA KHOO / POOJA SHIVANAND / HUSSEIN TAHA

Biodiversitas, Vol 23, Iss

Determination of crude oil degradation efficiency of biofilm producing bacteria isolated from oil contaminated site

2022 Volume 8

Abstract: ... was also found to have the highest degradation rate (0.0047 g/day) with a significant degradation of 7 ... had a lower rate of degradation (0.0033 g/day), resulting in a non-significant degradation ... Abstract. Khoo DH, Shivanand P, Taha H. 2022. Short Communication: Determination of crude oil ...

Abstract	Abstract. Khoo DH, Shivanand P, Taha H. 2022. Short Communication: Determination of crude oil degradation efficiency of biofilm producing bacteria isolated from oil contaminated site. Biodiversitas 23: 4138-4143. Microbes have gained much attention for their application in the bioremediation of petroleum contaminated areas, and biofilm producing bacteria are considered potential candidates for this purpose. The aim of this study was to screen six oil degrading bacterial strains for their ability to form biofilm, and to measure the crude oil degradation efficiency of selected strains. It was found that only two strains, Micrococcus sp. UBDBH15 and Pseudomonas sp. UBDBH26 were categorized as strong biofilm producers, with the latter showing the highest amount of biofilm formed. Under the conditions tested, Pseudomonas sp. UBDBH26 was also found to have the highest degradation rate (0.0047 g/day) with a significant degradation of 7.07 ± 3.02 and 11.38 ± 2.93 % of crude oil after 7 and 14 days respectively. Micrococcus sp. UBDBH15 had a lower rate of degradation (0.0033 g/day), resulting in a non-significant degradation. However, in comparison with a non-biofilm producer, Enterobacter sp. UBDBH06, this study suggested that biofilm might enhance the degradation of oil, but further studies are needed to confirm this assumption.
Keywords	bacteria ; biodegradation ; biofilm ; bioremediation ; petroleum ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	MBI & UNS Solo
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Stochastic Modelling of Symmetric Positive Definite Material Tensors

Shivanand, Sharana Kumar / Rosić, Bojana / Matthies, Hermann G.

2021

Abstract: Spatial symmetries and invariances play an important role in the behaviour of materials and should be respected in the description and modelling of material properties. The focus here is the class of physically symmetric and positive definite tensors, as ...

Abstract	Spatial symmetries and invariances play an important role in the behaviour of materials and should be respected in the description and modelling of material properties. The focus here is the class of physically symmetric and positive definite tensors, as they appear often in the description of materials, and one wants to be able to prescribe certain classes of spatial symmetries and invariances for each member of the whole ensemble, while at the same time demanding that the mean or expected value of the ensemble be subject to a possibly 'higher' spatial invariance class. We formulate a modelling framework which not only respects these two requirements$-$positive definiteness and invariance$-$but also allows a fine control over orientation on one hand, and strength/size on the other. As the set of positive definite tensors is not a linear space, but rather an open convex cone in the linear space of physically symmetric tensors, we consider it advantageous to widen the notion of mean to the so-called Fr\'echet mean on a metric space, which is based on distance measures or metrics between positive definite tensors other than the usual Euclidean one. It is shown how the random ensemble can be modelled and generated, independently in its scaling and orientational or directional aspects, with a Lie algebra representation via a memoryless transformation. The parameters which describe the elements in this Lie algebra are then to be considered as random fields on the domain of interest. As an example, a 2D and a 3D model of steady-state heat conduction in a human proximal femur, a bone with high material anisotropy, is modelled with a random thermal conductivity tensor, and the numerical results show the distinct impact of incorporating into the constitutive model different material uncertainties$-$scaling, orientation, and prescribed material symmetry$-$on the desired quantities of interest.
Keywords	Mathematics - Numerical Analysis ; Computer Science - Computational Engineering ; Finance ; and Science
Publishing date	2021-09-16
Publishing country	us
Document type	Book ; Online
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Article ; Online: Transcriptomic Insight into Aroma Pathway Genes and Effect of Ripening Difference on Expression of Aroma Genes in Different Mango Cultivars

Pathak, Garima / Dudhagi, Shivanand Suresh / Raizada, Saumya / Sane, Vidhu A.

Plant Mol Biol Rep. 2023 Mar., v. 41, no. 1 p.145-163

2023

Abstract: Mango is flavour-rich fruit and considered as king of fruit in India. About 578 volatile compounds have so far been identified in various cultivars. These are believed to accumulate differentially during the course of ripening. In order to understand the ...

Abstract	Mango is flavour-rich fruit and considered as king of fruit in India. About 578 volatile compounds have so far been identified in various cultivars. These are believed to accumulate differentially during the course of ripening. In order to understand the molecular mechanism of aroma in mango, de novo transcriptome assembly and analysis of Mangifera indica (Dashehari) were performed by Illumina sequencing. Mining of transcriptome data led to identification of major genes related to most of the genes of terpenoid, carotenoid, flavonoid, lactone, lipoxygenase, aromatic amino acid, alkaloid, and phenylpropanoid pathways, which are potentially involved in aroma biosynthesis. Comparative mRNA expression analysis in five Mango varieties (Dashehari, Banganpalli, Ratna, Mallika, and Alphonso) revealed varietal and ripening-related differences. To gain further insight into the terpenoid pathway, these genes were further studied in different tissues and developmental stages in Dashehari mango. This study is stepping stone to understand aroma pathways in different varieties of mango fruit.
Keywords	Mangifera indica ; alkaloids ; amino acids ; biosynthesis ; carotenoids ; cultivars ; flavonoids ; fruits ; gene expression ; lipoxygenases ; mangoes ; odors ; transcriptome ; transcriptomics ; India
Language	English
Dates of publication	2023-03
Size	p. 145-163.
Publishing place	Springer US
Document type	Article ; Online
ZDB-ID	2018592-3
ISSN	1572-9818 ; 0735-9640
ISSN (online)	1572-9818
ISSN	0735-9640
DOI	10.1007/s11105-022-01355-5
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Article ; Online: Genetic evidence for a single founding population of the Lakshadweep Islands.

Kumar, Sachin / Singh, Prajjval Pratap / Pasupuleti, Nagarjuna / Shendre, Shivanand S / Sequeira, Jaison Jeevan / Babu, Idrees / Mutak, Mohammed S / Rai, Niraj / Chaubey, Gyaneshwer

Molecular genetics and genomics : MGG

2024 Volume 299, Issue 1, Page(s) 8

Abstract: Lakshadweep is an archipelago of 36 islands located in the Southeastern Arabian Sea. In the absence of a detailed archaeological record, the human settlement timing of this island is vague. Previous genetic studies on haploid DNA makers suggested sex- ... ...

Abstract	Lakshadweep is an archipelago of 36 islands located in the Southeastern Arabian Sea. In the absence of a detailed archaeological record, the human settlement timing of this island is vague. Previous genetic studies on haploid DNA makers suggested sex-biased ancestry linked to North and South Indian populations. Maternal ancestry suggested a closer link with the Southern Indian, while paternal ancestry advocated the Northern Indian genetic affinity. Since the haploid markers are more sensitive to genetic drift, which is evident for the Island populations, we have used the biparental high-resolution single-nucleotide polymorphic markers to reconstruct the population history of Lakshadweep Islands. Using the fine-scaled analyses, we specifically focused on (A) the ancestry components of Lakshadweep Islands populations; (B) their relation with East, West Eurasia and South Asia; (C) the number of founding lineages and (D) the putative migration from Northern India as the paternal ancestry was closer to the North Indian populations. Our analysis of ancestry components confirmed relatively higher North Indian ancestry among the Lakshadweep population. These populations are closely related to the South Asian populations. We identified mainly a single founding population for these Islands, geographically divided into two sub-clusters. By examining the population's genetic composition and analysing the gene flow from different source populations, this study contributes to our understanding of Lakshadweep Island's evolutionary history and population dynamics. These findings shed light on the complex interactions between ethnic groups and their genetic contributions in making the Lakshadweep population.
MeSH term(s)	Humans ; Genetics, Population ; Ethnicity/genetics ; Asian People/genetics ; India ; Biological Evolution
Language	English
Publishing date	2024-02-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2044817-X
ISSN	1617-4623 ; 1617-4615
ISSN (online)	1617-4623
ISSN	1617-4615
DOI	10.1007/s00438-024-02110-z
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