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Article ; Online: Reproducing the scalp microbiota community: co-colonization of a 3D reconstructed human epidermis with C. acnes and M. restricta.

Meloni, Marisa / Balzaretti, Silvia / Collard, Nathalie / Desaint, Stephane / Laperdrix, Celine

International journal of cosmetic science

2021 Volume 43, Issue 2, Page(s) 235–245

Abstract: ... of the physiological barrier integrity and viability, though it shows aggregates. M. restricta formed large aggregates ... in barrier depletion, but the overall damage was less severe, respecting the single colonization with M ...

Abstract	Objective: A 3D reconstructed human epidermis (RHE) model colonized with specific microbial strains was developed to model the complex interactions between strains of the human scalp hair. Methods: Reconstructed human epidermis was colonized with Cutibacterium acnes and Malassezia restricta for 72 h. The epidermal model was characterized in terms of morphology, using immune-labelling targeting biomarkers for barrier structure, proliferation, differentiation and anti-microbial defence. The barrier function was assessed by transepithelial electrical eesistance (TEER) measurements. In order to study the microorganisms on the epidermal model, viable counts and phenotype ultrastructure analysis were performed by scanning electron microscopy (SEM). Results: The RHE colonized with C. acnes did not lead to severe modifications of the physiological barrier integrity and viability, though it shows aggregates. M. restricta formed large aggregates by a close interaction with the RHE, thus causing both a strong decrease in barrier function and structure degradation and an increased human beta defensin 2 (HBD2) expression. The co-colonized model resulted in barrier depletion, but the overall damage was less severe, respecting the single colonization with M. restricta. The developed 'scalp model' allowed to identify morphological modifications leading to uncontrolled epidermal renewal. Conclusion: This study shows a pre-clinical model that recapitulates the interactions that can occur between site-specific microbial strains and keratinocytes in dandruff condition. The model can be applied to assess ingredients and products' mechanism of action.
MeSH term(s)	Malassezia/isolation & purification ; Microbiota ; Propionibacteriaceae/isolation & purification ; Scalp/microbiology
Language	English
Publishing date	2021-03-11
Publishing country	England
Document type	Journal Article
ZDB-ID	198917-0
ISSN	1468-2494 ; 0142-5463
ISSN (online)	1468-2494
ISSN	0142-5463
DOI	10.1111/ics.12688
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Article ; Online: Rapid in vitro detection of CTX-M groups 1, 2, 8, 9 resistance genes by LAMP assays.

Rivoarilala, Odile Lalainasoa / Garin, Benoît / Andriamahery, Felamboahangy / Collard, Jean Marc

PloS one

2018 Volume 13, Issue 7, Page(s) e0200421

Abstract: Background: The prevalence of bacteria producing CTX-M Extended-Spectrum β-lactamases (ESBLs) has ... sensitive detection of the four most common CTX-M groups, namely CTX-M groups 1, 2, 8 and 9.: Methods ... LAMP primers targeting the four ESBLs CTX-M groups were designed using the Primer Explorer V4 software ...

Abstract	Background: The prevalence of bacteria producing CTX-M Extended-Spectrum β-lactamases (ESBLs) has increased around the world and some of them became a major cause of infections such as bloodstream or urinary tract infections (UTI). We developed a loop-mediated isothermal amplification (LAMP) assay for a simple, rapid and sensitive detection of the four most common CTX-M groups, namely CTX-M groups 1, 2, 8 and 9. Methods: LAMP primers targeting the four ESBLs CTX-M groups were designed using the Primer Explorer V4 software. The detection limit of the method was tested by serial dilution of reference DNAs. The primer specificity of the LAMP reaction was tested on DNA extracted from six strains producing various group of CTX-M and validated using DNA extracted from CTX-M-resistant clinical isolates (isolated from pus, urine, or blood). Results were compared with those of conventional PCR. Results: We were able to detect down to 0.1 pg/ul of DNA using the newly developed LAMP assays whereas the minimal amount detectable for conventional PCR was 50 to 100pg/ul, indicating that the LAMP assay was found to have a detection limit at least 500 to 1000 times lower than the PCR. Additionally, representative genes from the CTX-M groups 1, 2, 8 and 9 were amplified using the designed assay and no cross amplification was observed between the four CTX-M groups, demonstrating the specificity of the LAMP assay. Of the 37 clinical strains tested, the four LAMP assays showed 100% sensitivity and 87%, 97%, 100%, 100% specificity for the CTX-M groups 1, 2, 8 and 9 respectively. Conclusion: Being sensitive, specific, rapid and standard methods, the LAMP assays developed in this study have a potential to be beneficial tools in molecular epidemiology and surveillance studies of the four prevalent EBSLs CTX-M groups even in low cost laboratory.
MeSH term(s)	Bacterial Proteins/analysis ; Bacterial Proteins/genetics ; Enterobacteriaceae Infections/diagnosis ; Escherichia coli ; Humans ; Nucleic Acid Amplification Techniques/methods ; Salmonella ; Sensitivity and Specificity ; Time Factors ; beta-Lactamases/analysis ; beta-Lactamases/genetics
Chemical Substances	Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2018-07-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Validation Studies
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0200421
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Article: Hate in the time of the Covid-19 pandemic: dehumanisation as a side effect;

Collard, Melanie

Crime, law, and social change

2023 , Page(s) 1–19

Abstract: This article is about denouncing the dehumanisation process that took place in the time of Covid-19. It recognises that governments have a vital role to play in setting national directions to tackle racist violence and that the value of having hate crime ...

Abstract	This article is about denouncing the dehumanisation process that took place in the time of Covid-19. It recognises that governments have a vital role to play in setting national directions to tackle racist violence and that the value of having hate crime laws should not be underestimated. However, it argues that a broader approach is needed to embark upon a
Language	English
Publishing date	2023-01-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2015096-9
ISSN	1573-0751 ; 0925-4994
ISSN (online)	1573-0751
ISSN	0925-4994
DOI	10.1007/s10611-022-10073-8
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Article ; Online: Contributions of anesthesiology to the surgical treatment of cerebrovascular disease: the role of Arthur S. Keats, M.D.

Collard, Charles D / Anton, James M / Cooper, John R / Giesecke, N Martin

Anesthesiology

2008 Volume 108, Issue 4, Page(s) 756–758

Abstract: Increased tolerance to cerebral ischemia produced by general anesthesia during temporary carotid occlusion. By B. A. Wells, A. S. Keats, and D. A. Cooley. Surgery 1963; 54:216-23. Local anesthesia with little or no preoperative sedation is currently ... ...

Abstract	Increased tolerance to cerebral ischemia produced by general anesthesia during temporary carotid occlusion. By B. A. Wells, A. S. Keats, and D. A. Cooley. Surgery 1963; 54:216-23. Local anesthesia with little or no preoperative sedation is currently recommended as the anesthetic of choice for temporary carotid occlusion during carotid endarterectomy. Purported advantages include minimal circulatory and respiratory changes from the local anesthetic, and constant verbal contact can be maintained with the patient so that neurologic changes are promptly recognized. However, local anesthesia may not be satisfactory in uncooperative or semiconscious patients. We therefore undertook a trial of general anesthesia in 56 consecutive patients undergoing carotid endarterectomy. Patients were induced in standardized fashion using intravenous thiopental (100-400 mg), atropine (0.2 mg), and succinylcholine (40-80 mg). Cyclopropane, along with deliberate hypercapnia and hypertension, was used for anesthesia maintenance. All patients tolerated carotid occlusion for periods of up to 30 min during general anesthesia without shunt, bypass, or hypothermia. Except for one patient, electroencephalogram evidence of cerebral ischemia was not apparent during occlusion, and no patient suffered postoperative neurologic sequela. Twenty percent of patients who had their carotid arteries occluded preoperatively for 30-60 s without general anesthesia suffered convulsions. These data suggest that general anesthesia increased the tolerance to cerebral ischemia. Potential mechanisms involved might include: 1) decreased cerebral metabolic rate for oxygen; 2) increased cerebral blood flow from hypercapnia; 3) increased arterial oxygen tension; and 4) recruitment of new routes of collateral circulation.
MeSH term(s)	Anesthesiology/history ; Anesthesiology/methods ; Animals ; Cerebrovascular Disorders/history ; Cerebrovascular Disorders/surgery ; History, 19th Century ; History, 20th Century ; Humans
Language	English
Publishing date	2008-04
Publishing country	United States
Document type	Historical Article ; Journal Article
ZDB-ID	269-0
ISSN	1528-1175 ; 0003-3022
ISSN (online)	1528-1175
ISSN	0003-3022
DOI	10.1097/ALN.0b013e3181672648
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Article: Detection and characterization of tet(M) in tetracycline-resistant Listeria strains from human and food-processing origins in Belgium and France.

Bertrand, Sophie / Huys, Geert / Yde, Marc / D'Haene, Klaas / Tardy, Florence / Vrints, Martine / Swings, Jean / Collard, Jean-Marc

Journal of medical microbiology

2005 Volume 54, Issue Pt 12, Page(s) 1151–1156

Abstract: ... to tetracycline (TC) due to the presence of the tet(M) gene. Through sequence analysis, it was shown that tet(M ... chromosomally encoded tet(M) genes previously found in Staphylococcus aureus and in lactobacilli. The tet(M ... of conjugative transposons and were closely related to SHG III, which harbours enterococcal tet(M) genes ...

Abstract	In the present study, three Listeria monocytogenes strains and one Listeria innocua strain out of a collection of 241 Listeria isolates from human and food-processing sources were found to display resistance to tetracycline (TC) due to the presence of the tet(M) gene. Through sequence analysis, it was shown that tet(M) genes in two of the isolates belong to sequence homology group (SHG) II, a group comprising chromosomally encoded tet(M) genes previously found in Staphylococcus aureus and in lactobacilli. The tet(M) genes of the two other L. monocytogenes strains were associated with a member of the Tn916-Tn1545 family of conjugative transposons and were closely related to SHG III, which harbours enterococcal tet(M) genes associated with Tn916. One of these transposon-containing strains was able to transfer the tet(M) gene to Enterococcus faecalis recipient strain JH2-2. Collectively, these sequence and conjugation data indicate that the acquisition of tet(M) by Listeria strains may be triggered by successive transfers between other Gram-positive organisms.
MeSH term(s)	Base Sequence ; Belgium ; DNA Primers ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Food Microbiology ; France ; Humans ; Listeria/classification ; Listeria/drug effects ; Listeria/genetics ; Listeria/isolation & purification ; Listeriosis/epidemiology ; Molecular Sequence Data ; Polymerase Chain Reaction ; Tetracycline/pharmacology ; Tetracycline Resistance/genetics
Chemical Substances	DNA Primers ; DNA, Bacterial ; Tetracycline (F8VB5M810T)
Language	English
Publishing date	2005-11-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218356-0
ISSN	1473-5644 ; 0022-2615
ISSN (online)	1473-5644
ISSN	0022-2615
DOI	10.1099/jmm.0.46142-0
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Article: Clonal emergence of extended-spectrum beta-lactamase (CTX-M-2)-producing Salmonella enterica serovar Virchow isolates with reduced susceptibilities to ciprofloxacin among poultry and humans in Belgium and France (2000 to 2003).

Bertrand, Sophie / Weill, François-Xavier / Cloeckaert, Axel / Vrints, Martine / Mairiaux, Eric / Praud, Karine / Dierick, Katlijne / Wildemauve, Christa / Godard, Claudine / Butaye, Patrick / Imberechts, Hein / Grimont, Patrick A D / Collard, Jean-Marc

Journal of clinical microbiology

2006 Volume 44, Issue 8, Page(s) 2897–2903

Abstract: ... found to produce an extended-spectrum beta-lactamase, CTX-M-2, concomitantly with a TEM-1 beta-lactamase ... The bla(CTX-M-2) gene was located on a large conjugative plasmid (>100 kb ... susceptibility to ciprofloxacin (MICs of between 0.5 and 1 mug/ml). CTX-M-2-producing Salmonella with a reduced ...

Abstract	Antibiotic treatment is not required in cases of Salmonella enterica gastroenteritis but is essential in cases of enteric fever or invasive salmonellosis or in immunocompromised patients. Although fluoroquinolones and extended-spectrum cephalosporins are the drugs of choice to treat invasive Salmonella, resistance to these antibiotics is increasing worldwide. During the period 2000 to 2003, 90 Salmonella enterica serovar Virchow poultry and poultry product isolates and 11 serovar Virchow human isolates were found to produce an extended-spectrum beta-lactamase, CTX-M-2, concomitantly with a TEM-1 beta-lactamase. The bla(CTX-M-2) gene was located on a large conjugative plasmid (>100 kb). Pulsed-field gel electrophoresis indicated a clonal relationship between the poultry and human isolates. All these isolates displayed additional resistance to trimethoprim-sulfamethoxazole and tetracycline as well as a reduced susceptibility to ciprofloxacin (MICs of between 0.5 and 1 mug/ml). CTX-M-2-producing Salmonella with a reduced susceptibility to fluoroquinolones constitutes a major concern, since such strains could disseminate on a large scale and jeopardize classical antibiotic therapy in immunocompromised patients.
MeSH term(s)	Animals ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/analysis ; Bacterial Proteins/genetics ; Bacterial Typing Techniques ; Belgium ; Chromosomes, Bacterial/genetics ; Ciprofloxacin/pharmacology ; Cluster Analysis ; DNA Fingerprinting ; DNA, Bacterial/genetics ; Drug Resistance, Multiple, Bacterial/genetics ; Electrophoresis, Gel, Pulsed-Field ; France ; Genome, Bacterial/genetics ; Genotype ; Humans ; Meat Products/microbiology ; Microbial Sensitivity Tests ; Plasmids ; Polymorphism, Restriction Fragment Length ; Poultry ; Poultry Diseases/microbiology ; Salmonella Infections/microbiology ; Salmonella Infections, Animal/microbiology ; Salmonella enterica/classification ; Salmonella enterica/drug effects ; Salmonella enterica/enzymology ; Salmonella enterica/isolation & purification ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; DNA, Bacterial ; Ciprofloxacin (5E8K9I0O4U) ; beta-lactamase CTX-2 (EC 3.5.2.-) ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2006-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.02549-05
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Article: Apport de l'I.R.M. au diagnostic topographique des troubles oculomoteurs dans la sclérose en plaques.

Rumbach, L / Eber, A M / Dietemann, J L / Bataillard, M / Tranchant, C / Warter, J M / Collard, M

Revue neurologique

1990 Volume 146, Issue 1, Page(s) 30–35

Abstract: Magnetic resonance imaging (MRI) was performed in 20 patients with multiple sclerosis and abnormal electro-oculographic examination. All but 2 patients showed MRI abnormalities in the infratentorial region: hypersignal on T2-weighted sequences and/or ... ...

Title translation	Contribution of MRI to the topography of oculomotor disorders in multiple sclerosis.
Abstract	Magnetic resonance imaging (MRI) was performed in 20 patients with multiple sclerosis and abnormal electro-oculographic examination. All but 2 patients showed MRI abnormalities in the infratentorial region: hypersignal on T2-weighted sequences and/or images of atrophy. Usually, each patient had multiple abnormalities, which could prevent anatomico-oculographic correlations. With oculomotor disorders of cerebellar origin, correlations between clinical findings and MRI images were satisfactory, but with disorders due to brainstem lesions correlations were not so good, as shown by the results in 9 patients with internuclear ophthalmoplegia.
MeSH term(s)	Adult ; Brain/pathology ; Electrooculography ; Female ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Multiple Sclerosis/complications ; Ocular Motility Disorders/diagnosis ; Ocular Motility Disorders/etiology ; Retrospective Studies
Language	French
Publishing date	1990
Publishing country	France
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	4593-7
ISSN	2213-0004 ; 0035-3787
ISSN (online)	2213-0004
ISSN	0035-3787
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Article ; Online: Power, intersectionality and stigma: Informing a gender- and spatially-sensitive public health approach to women and gambling in Great Britain.

Fannin, Maria / Collard, Sharon / Davies, Sara

Health & place

2024 Volume 86, Page(s) 103186

Abstract: In Britain more men participate in gambling than women, although the gender gap is narrowing; and online gambling is increasing among women and men. Gambling practices differ between men and women but also between different groups of women, with evidence ...

Abstract	In Britain more men participate in gambling than women, although the gender gap is narrowing; and online gambling is increasing among women and men. Gambling practices differ between men and women but also between different groups of women, with evidence that younger women are diversifying to gamble in different ways from older women. Complex and powerful spatial, socio-cultural and economic forces shape women's experiences of gambling, the problems gambling may cause, and wider societal efforts to minimise these harms. This paper presents the findings of a Rapid Evidence Assessment (REA) of research on women and gambling and conceptual reflections on these findings to argue for greater attention to the gendered and spatial dimensions of gambling in three ways. First, we discuss the geographical focus and scope of the research we reviewed on women and gambling; second, we analyse the changing geographies of where women gamble; and finally, we consider how qualitative notions of space as experiential and co-constitutive can offer a more nuanced conceptual framework for understanding women's lived experience of gambling and gambling harm. We identify areas for further research on gambling and gambling harm that attends to gendered and spatial dimensions of gambling, including online spaces; the intersectional dynamics that shape gambling practice and gambling harms; and the experiences of those affected by others' gambling.
MeSH term(s)	Male ; Humans ; Female ; Aged ; Public Health ; Gambling ; United Kingdom ; Intersectional Framework ; Social Stigma
Language	English
Publishing date	2024-02-29
Publishing country	England
Document type	Review ; Journal Article
ZDB-ID	1262540-1
ISSN	1873-2054 ; 1353-8292
ISSN (online)	1873-2054
ISSN	1353-8292
DOI	10.1016/j.healthplace.2024.103186
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Article ; Online: Mental training is crucial to preparedness for athletes, special forces, musicians… and surgeons.

Collard, M K / Honoré, C

Journal of visceral surgery

2022 Volume 159, Issue 2, Page(s) 87–88

MeSH term(s)	Humans ; Surgeons ; Surveys and Questionnaires
Language	English
Publishing date	2022-06-14
Publishing country	France
Document type	Editorial
ISSN	1878-7886
ISSN (online)	1878-7886
DOI	10.1016/j.jviscsurg.2022.03.001
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Article ; Online: An assessment of the impact of cross-cultural variation in plant macronutrients on the recommendations of the Paleo Diet.

Ruffett, Amalea / Collard, Mark

The American journal of clinical nutrition

2022 Volume 117, Issue 4, Page(s) 777–784

Abstract: Background: One of the main recommendations of the Paleo Diet is that individuals replicate the whole-diet macronutrient ranges of hunter-gatherer diets. These are suggested to be 19%-35% protein, 22%-40% carbohydrate, and 28%-58% fat, by energy. ... ...

Abstract	Background: One of the main recommendations of the Paleo Diet is that individuals replicate the whole-diet macronutrient ranges of hunter-gatherer diets. These are suggested to be 19%-35% protein, 22%-40% carbohydrate, and 28%-58% fat, by energy. However, the plant food contribution to these ranges was estimated exclusively from Australian data, which is a potential problem. Objectives: We investigated whether estimates of the contribution of protein, carbohydrate, and fat to hunter-gatherer diets are impacted by using plant data from other regions of the world. Methods: The values that form the basis of the Paleo Diet' s recommended macronutrient ranges were generated with a set of equations. We combined these equations with published plant macronutrient data from a multi-region sample of 5 hunter-gatherer groups to generate new estimated macronutrient percentages and then statistically compared the old and new estimates. Subsequently, we collated plant macronutrient data for a sample of 10 hunter-gatherer groups from several regions and repeated the exercise. Results: The whole-diet macronutrient percentages we calculated are significantly different from those that underpin the Paleo Diet's recommendations. Additionally, the ranges derived from our whole-diet macronutrient percentages (14%-35% protein, 21%-55% carbohydrate, 12%-58% fat) are markedly wider than those recommended by the Paleo Diet. Conclusions: The estimated whole-diet macronutrient percentages that form the basis of the Paleo Diet's macronutrient recommendations are not robust. Using plant data from multiple regions leads to significantly different estimates. Additionally, the macronutrient ranges derived from our whole-diet macronutrient percentages overlap with those recommended by the US Department of Agriculture and the WHO. This undercuts one of the main justifications for adopting the Paleo Diet-namely that because it is vastly different from Western diets, it can reduce the probability of experiencing noncommunicable diseases. There may still be reasons for adopting the Paleo Diet rather than a conventional diet, but healthier macronutrient percentages is not one of them.
MeSH term(s)	Humans ; Diet, Paleolithic ; Cross-Cultural Comparison ; Australia ; Diet ; Nutrients ; Diet, Western ; Carbohydrates
Chemical Substances	Carbohydrates
Language	English
Publishing date	2022-12-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	280048-2
ISSN	1938-3207 ; 0002-9165
ISSN (online)	1938-3207
ISSN	0002-9165
DOI	10.1016/j.ajcnut.2022.12.003
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