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Article ; Online: Sex differences in places of home care.

Yamanaka, Takashi / Kidana, Kiwami / Mizuki, Maiko / Hattori, Yukari / Hamada, Shota / Akishita, Masahiro

2022 Volume 22, Issue 9, Page(s) 813–814

MeSH term(s)	Female ; Home Care Services ; Humans ; Male ; Sex Characteristics
Language	English
Publishing date	2022-07-25
Publishing country	Japan
Document type	Letter
ZDB-ID	2113849-7
ISSN	1447-0594 ; 1444-1586
ISSN (online)	1447-0594
ISSN	1444-1586
DOI	10.1111/ggi.14444
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Article ; Online: Improvement of the mismatch amplification mutation assay-PCR for discrimination between Streptococcus suis serotypes 2 and 1/2.

Okuhama-Yoshida, Erina / Nakayama, Mizuki / Hattori, Miki / Takamatsu, Daisuke / Okura, Masatoshi

Journal of microbiological methods

2023 Volume 214, Page(s) 106828

Abstract: A mismatch amplification mutation assay (MAMA)-PCR, which detects a single-nucleotide polymorphism contributed to serological difference between Streptococcus suis serotypes 2 and 1/2, is used to discriminate between these serotypes. The present study ... ...

Abstract	A mismatch amplification mutation assay (MAMA)-PCR, which detects a single-nucleotide polymorphism contributed to serological difference between Streptococcus suis serotypes 2 and 1/2, is used to discriminate between these serotypes. The present study reports unusual serotype 1/2 isolates untypable by the MAMA-PCR and improvement of the MAMA-PCR for typing such isolates.
MeSH term(s)	Humans ; Animals ; Swine ; Serogroup ; Serotyping ; Streptococcus suis/genetics ; Streptococcal Infections/diagnosis ; Polymerase Chain Reaction ; Mutation ; Swine Diseases/diagnosis
Language	English
Publishing date	2023-09-30
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2023.106828
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Article: Anatomical study of the bone morphology of the anterior talofibular ligament attachment.

Fujishiro, Hitomi / Nimura, Akimoto / Azumaya, Mizuki / Hattori, Soichi / Hoshi, Osamu / Akita, Keiichi

Anatomy & cell biology

2023 Volume 56, Issue 3, Page(s) 334–341

Abstract: Anterior talofibular ligament (ATFL) injuries are the most common cause of ankle sprains. To ensure anatomically accurate surgery and ultrasound imaging of the ATFL, anatomical knowledge of the bony landmarks around the ATFL attachment to the distal ... ...

Abstract	Anterior talofibular ligament (ATFL) injuries are the most common cause of ankle sprains. To ensure anatomically accurate surgery and ultrasound imaging of the ATFL, anatomical knowledge of the bony landmarks around the ATFL attachment to the distal fibula is required. The purpose of the present study was to anatomically investigate the ATFL attachment to the fibula with respect to bone morphology and attachment structures. First, we analyzed 36 feet using microcomputed tomography. After excluding 9 feet for deformities, the remaining 27 feet were used for chemically debrided bone analysis and macroscopic and histological observations. Ten feet of living specimens were observed using ultrasonography. We found that a bony ridge was present at the boundary between the attachments of the ATFL and calcaneofibular ligament (CFL) to the fibula. These two attachments could be distinguished based on a difference in fiber orientation. Histologically, the ATFL was attached to the anterodistal part of the fibula via fibrocartilage anterior to the bony ridge indicating the border with the CFL attachment. Using ultrasonography in living specimens, the bony ridge and hyperechoic fibrillar pattern of the ATFL could be visualized. We established that the bony ridge corresponded to the posterior margin of the ATFL attachment itself. The ridge was obvious, and the superior fibers of the ATFL have directly attached anteriorly to it. This bony ridge could become a valuable and easy-to-use landmark for ultrasound imaging of the ATFL attachment if combined with the identification of the fibrillar pattern of the ATFL.
Language	English
Publishing date	2023-07-24
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2590173-4
ISSN	2093-3673 ; 2093-3665
ISSN (online)	2093-3673
ISSN	2093-3665
DOI	10.5115/acb.23.002
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Article ; Online: Investigation of emission plane control in GaInN/GaN multiple-quantum shells for efficient nanowire-based LEDs.

Inaba, Soma / Lu, Weifang / Shima, Ayaka / Ii, Shiori / Takahashi, Mizuki / Yamanaka, Yuki / Hattori, Yuta / Kubota, Kosei / Huang, Kai / Iwaya, Motoaki / Takeuchi, Tetsuya / Kamiyama, Satoshi

Nanoscale advances

2024 Volume 6, Issue 9, Page(s) 2306–2318

Abstract: Significant attention has been directed toward core-shell GaInN/GaN multiple-quantum shell (MQS) nanowires (NWs) in the context of high-efficiency micro light-emitting diodes (micro-LEDs). These independent three-dimensional NWs offer the advantage of ... ...

Abstract	Significant attention has been directed toward core-shell GaInN/GaN multiple-quantum shell (MQS) nanowires (NWs) in the context of high-efficiency micro light-emitting diodes (micro-LEDs). These independent three-dimensional NWs offer the advantage of reducing the impact of sidewall etching regions. Furthermore, the emitting plane on the sidewalls demonstrates either nonpolar or semipolar orientation, while the dislocation density is exceptionally low. In this study, we assessed how changes in the NW morphology are affected by GaInN/GaN superlattice (SL) structures grown at varying growth temperatures, as well as control of the emission plane
Language	English
Publishing date	2024-03-27
Publishing country	England
Document type	Journal Article
ISSN	2516-0230
ISSN (online)	2516-0230
DOI	10.1039/d3na01101a
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Article ; Online: Insights into the catalytic mechanism of Grimontia hollisae collagenase through structural and mutational analyses.

Ueshima, Saori / Yasumoto, Mizuki / Kitagawa, Yuto / Akazawa, Kaho / Takita, Teisuke / Tanaka, Keisuke / Hattori, Shunji / Mizutani, Kimihiko / Mikami, Bunzo / Yasukawa, Kiyoshi

FEBS letters

2023 Volume 597, Issue 19, Page(s) 2473–2483

Abstract: Grimontia hollisae collagenase (Ghcol) exhibits high collagen-degrading activity. To explore its catalytic mechanism, its substrate (Gly-Pro-Hyp-Gly-Pro-Hyp, GPOGPO)-complexed crystal structure was determined at 2.0 Å resolution. A water molecule was ... ...

Abstract	Grimontia hollisae collagenase (Ghcol) exhibits high collagen-degrading activity. To explore its catalytic mechanism, its substrate (Gly-Pro-Hyp-Gly-Pro-Hyp, GPOGPO)-complexed crystal structure was determined at 2.0 Å resolution. A water molecule was observed near the active-site zinc ion. Since this water was not observed in the product (GPO)-complexed Ghcol, it was hypothesized that the GPOGPO-complexed Ghcol structure reflects a Michaelis complex, providing a structural basis for understanding the catalytic mechanism. Analyses of the active-site geometry and site-directed mutagenesis of the active-site tyrosine residues revealed that Glu493 and Tyr564 were essential for catalysis, suggesting that Glu493 functions as an acid and base catalyst while Tyr564 stabilizes the tetrahedral complex in the transition state. These results shed light on the catalytic mechanism of bacterial collagenase.
Language	English
Publishing date	2023-09-25
Publishing country	England
Document type	Journal Article
ZDB-ID	212746-5
ISSN	1873-3468 ; 0014-5793
ISSN (online)	1873-3468
ISSN	0014-5793
DOI	10.1002/1873-3468.14732
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Article: Lower limb muscle strengthening exercises in patients with early-stage amyotrophic lateral sclerosis: a case series study.

Kato, Naoki / Hashida, Goichi / Kobayashi, Mizuki / Hattori, Noriaki

Journal of physical therapy science

2020 Volume 32, Issue 10, Page(s) 615–620

Abstract: Purpose] We investigated whether patients with early-stage amyotrophic lateral sclerosis can improve their voluntary strength with a physical therapy program. [Participants and Methods] This retrospective case series study at a single university ... ...

Abstract	[Purpose] We investigated whether patients with early-stage amyotrophic lateral sclerosis can improve their voluntary strength with a physical therapy program. [Participants and Methods] This retrospective case series study at a single university hospital included 13 patients with amyotrophic lateral sclerosis (amyotrophic lateral sclerosis functional rating scale-revised ≥35, modified functional ambulation categories score ≥4). Physical therapy was performed for 3 weeks. We investigated knee extension muscle strength and modified functional ambulation categories scores at the start and end of the therapy and calculated the improvement rate of knee extension muscle strength. We performed a regression analysis of the relationship between knee extension muscle strength at the start of the study and the improvement rate. [Results] The knee extension muscle strength improved significantly; however, the effect size was small (0.13). The modified functional ambulation categories scores did not improve significantly. The knee extension muscle strength at the start of the therapy was negatively correlated with the improvement rate (logarithmic transformed linear regression: adjusted R
Language	English
Publishing date	2020-10-03
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2038898-6
ISSN	0915-5287
ISSN	0915-5287
DOI	10.1589/jpts.32.615
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Article ; Online: Insights into the catalytic mechanism of Grimontia hollisae collagenase through structural and mutational analyses

Ueshima, Saori / Yasumoto, Mizuki / Kitagawa, Yuto / Akazawa, Kaho / Takita, Teisuke / Tanaka, K. / Hattori, Shunji / Mizutani, Kimihiko / Mikami, Bunzo / Yasukawa, Kiyoshi

FEBS Letters. 2023 Oct., v. 597, no. 19 p.2473-2483

2023

Abstract: Grimontia hollisae collagenase (Ghcol) exhibits high collagen‐degrading activity. To explore its catalytic mechanism, its substrate (Gly‐Pro‐Hyp‐Gly‐Pro‐Hyp, GPOGPO)‐complexed crystal structure was determined at 2.0 Å resolution. A water molecule was ... ...

Abstract	Grimontia hollisae collagenase (Ghcol) exhibits high collagen‐degrading activity. To explore its catalytic mechanism, its substrate (Gly‐Pro‐Hyp‐Gly‐Pro‐Hyp, GPOGPO)‐complexed crystal structure was determined at 2.0 Å resolution. A water molecule was observed near the active‐site zinc ion. Since this water was not observed in the product (GPO)‐complexed Ghcol, it was hypothesized that the GPOGPO‐complexed Ghcol structure reflects a Michaelis complex, providing a structural basis for understanding the catalytic mechanism. Analyses of the active‐site geometry and site‐directed mutagenesis of the active‐site tyrosine residues revealed that Glu493 and Tyr564 were essential for catalysis, suggesting that Glu493 functions as an acid and base catalyst while Tyr564 stabilizes the tetrahedral complex in the transition state. These results shed light on the catalytic mechanism of bacterial collagenase.
Keywords	active sites ; catalysts ; catalytic activity ; collagenase ; crystal structure ; geometry ; site-directed mutagenesis ; tyrosine ; zinc
Language	English
Dates of publication	2023-10
Size	p. 2473-2483.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	212746-5
ISSN	1873-3468 ; 0014-5793
ISSN (online)	1873-3468
ISSN	0014-5793
DOI	10.1002/1873-3468.14732
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Article ; Online: Crystal structure of Grimontia hollisae collagenase provides insights into its novel substrate specificity toward collagen.

Ikeuchi, Takeaki / Yasumoto, Mizuki / Takita, Teisuke / Tanaka, Keisuke / Kusubata, Masashi / Hayashida, Osamu / Hattori, Shunji / Mizutani, Kimihiko / Mikami, Bunzo / Yasukawa, Kiyoshi

The Journal of biological chemistry

2022 Volume 298, Issue 8, Page(s) 102109

Abstract: Collagenase from the gram-negative bacterium Grimontia hollisae strain 1706B (Ghcol) degrades collagen more efficiently even than clostridial collagenase, the most widely used industrial collagenase. However, the structural determinants facilitating this ...

Abstract	Collagenase from the gram-negative bacterium Grimontia hollisae strain 1706B (Ghcol) degrades collagen more efficiently even than clostridial collagenase, the most widely used industrial collagenase. However, the structural determinants facilitating this efficiency are unclear. Here, we report the crystal structures of ligand-free and Gly-Pro-hydroxyproline (Hyp)-complexed Ghcol at 2.2 and 2.4 Å resolution, respectively. These structures revealed that the activator and peptidase domains in Ghcol form a saddle-shaped structure with one zinc ion and four calcium ions. In addition, the activator domain comprises two homologous subdomains, whereas zinc-bound water was observed in the ligand-free Ghcol. In the ligand-complexed Ghcol, we found two Gly-Pro-Hyp molecules, each bind at the active site and at two surfaces on the duplicate subdomains of the activator domain facing the active site, and the nucleophilic water is replaced by the carboxyl oxygen of Hyp at the P1 position. Furthermore, all Gly-Pro-Hyp molecules bound to Ghcol have almost the same conformation as Pro-Pro-Gly motif in model collagen (Pro-Pro-Gly)
MeSH term(s)	Bacterial Proteins/chemistry ; Collagen/chemistry ; Collagenases/chemistry ; Hydroxyproline/chemistry ; Substrate Specificity ; Vibrionaceae/enzymology ; Water/chemistry ; Zinc/chemistry
Chemical Substances	Bacterial Proteins ; Water (059QF0KO0R) ; Collagen (9007-34-5) ; Collagenases (EC 3.4.24.-) ; Zinc (J41CSQ7QDS) ; Hydroxyproline (RMB44WO89X)
Language	English
Publishing date	2022-06-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1016/j.jbc.2022.102109
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Article ; Online: Corrigendum to: Twinned/untwinned catalytic gold nanoparticles identified by applying a convolutional neural network to their Hough transformed Z-contrast images.

Yamamoto, Yuta / Hattori, Mizuki / Ohyama, Junya / Satsuma, Atsushi / Tanaka, Nobuo / Muto, Shunsuke

Microscopy (Oxford, England)

2019 Volume 68, Issue 2, Page(s) 194

Language	English
Publishing date	2019-02-21
Publishing country	England
Document type	Journal Article ; Published Erratum
ZDB-ID	2707496-1
ISSN	2050-5701 ; 2050-5698
ISSN (online)	2050-5701
ISSN	2050-5698
DOI	10.1093/jmicro/dfz008
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Article ; Online: Analysis of the reliability of rapid diagnostic tests for varicella, including breakthrough cases.

Higashimoto, Yuki / Hattori, Fumihiko / Kawamura, Yoshiki / Kozawa, Kei / Hamano, Aoi / Kato, Mizuki / Kato, Sayaka / Hosokawa, Asuka / Enya, Yasuko / Ihira, Masaru / Yoshikawa, Tetsushi

Journal of medical virology

2023 Volume 95, Issue 2, Page(s) e28569

Abstract: In the era of universal varicella vaccination, diagnosis of varicella is challenging, especially for breakthrough cases. We sought to clarify the reliability of direct varicella-zoster virus (VZV) loop-mediated isothermal amplification (LAMP) and ... ...

Abstract	In the era of universal varicella vaccination, diagnosis of varicella is challenging, especially for breakthrough cases. We sought to clarify the reliability of direct varicella-zoster virus (VZV) loop-mediated isothermal amplification (LAMP) and DermaQuick® VZV using the immunochromatography technique as rapid diagnostic tests for varicella. In addition, the usefulness of saliva as a sample type for direct LAMP was investigated. Among the 46 enrolled patients with suspected VZV infection, 31 patients (67.3%) were positive for the nucleic acid test based on real-time PCR from skin swab samples. Direct LAMP of skin swabs was positive in 29 (63.0%) of 46 patients. DermaQuick® VZV was positive in 25 (54.3%) of 46 patients. VZV DNA was detected in only 48.4% of oral swabs with the direct LAMP method. With real-time polymerase chain reaction (PCR) as the standard for diagnosing varicella, the sensitivity and specificity of DermaQuick® VZV were 80.7% and 100%, respectively. The sensitivity and specificity of direct LAMP from skin swabs were 93.6% and 100%, respectively. The sensitivity and specificity of real-time PCR for DNA extracted from oral swabs were 74.2% and 93.3%, respectively. Thus, oral swab samples are not suitable for breakthrough varicella diagnosis. Although DermaQuick® VZV is considered the most convenient point-of-care test for varicella, its sensitivity and specificity were lower than those of direct VZV LAMP.
MeSH term(s)	Humans ; Herpesvirus 3, Human/genetics ; Chickenpox ; Rapid Diagnostic Tests ; Reproducibility of Results ; DNA, Viral/genetics ; Herpes Zoster
Chemical Substances	DNA, Viral
Language	English
Publishing date	2023-02-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.28569
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