Article ; Online: Xiaoyu Xiezhuo Drink Protects against Ischemia-Reperfusion Acute Kidney Injury in Aged Mice through Inhibiting the TGF-
2021 Volume 2021, Page(s) 9963732
Abstract: ... that is especially troubling aged people. An effective self-made Chinese medicine formula, Xiaoyu Xiezhuo ...
| Abstract | Acute kidney injury (AKI) is responsible for significant mortality among hospitalized patients that is especially troubling aged people. An effective self-made Chinese medicine formula, Xiaoyu Xiezhuo Drink (XXD), displayed therapeutic effects on AKI. However, the compositions and underlying mechanisms of XXD remain to be elucidated. In this study, we used the ultra-high-performance liquid chromatography method coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) to investigate the chemical components in XXD. Then, the absorbable components of XXD were identified based on the five principles and inputted into the SwissTargetPrediction and STITCH databases to identify the drug targets. AKI-related targets were collected from the GenCLiP 3, GeneCards, and DisGeNET databases. The crossover genes of XXD and AKI were identified for functional enrichment analysis. The protein-protein interaction (PPI) network of crossover genes was constructed, followed by the identification of hub genes. Subsequently, the effects and potential mechanisms of XXD on AKI predicted by the network pharmacology and bioinformatics analyses were experimentally validated in ischemia-reperfusion (I/R) injury-induced AKI aged mouse models. A total of 122 components in XXD were obtained; among them, 58 components were found that could be absorbed in the blood. There were 800 potential drug targets predicted from the 58 absorbable components in AKI which shared 36 crossover genes with AKI-related targets. The results of functional enrichment analysis indicated that crossover genes mostly associated with the response to oxidative stress and the HIF1 signaling pathway. In the PPI network analysis, 12 hub genes were identified, including ALB, IL-6, TNF, TP53, VEGFA, PTGS2, TLR4, NOS3, EGFR, PPARG, HIF1A, and HMOX1. In AKI aged mice, XXD prominently alleviated I/R injury-induced renal dysfunction, abnormal renal pathological changes, and cellular senescence, inflammation, and oxidative damage with a reduction in the expression level of the inflammatory mediator, |
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| MeSH term(s) | Acute Kidney Injury/drug therapy ; Acute Kidney Injury/metabolism ; Acute Kidney Injury/physiopathology ; Animals ; China ; Chromatography, High Pressure Liquid/methods ; Disease Models, Animal ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/metabolism ; Drugs, Chinese Herbal/pharmacology ; Ischemia/pathology ; Kidney/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Protective Agents/pharmacology ; Reperfusion ; Reperfusion Injury/drug therapy ; Reperfusion Injury/metabolism ; Reperfusion Injury/prevention & control ; Signal Transduction/drug effects ; Tandem Mass Spectrometry/methods |
| Chemical Substances | Drugs, Chinese Herbal ; Protective Agents ; xiaoyu zhixue |
| Language | English |
| Publishing date | 2021-09-09 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2698540-8 |
| ISSN | 2314-6141 ; 2314-6133 |
| ISSN (online) | 2314-6141 |
| ISSN | 2314-6133 |
| DOI | 10.1155/2021/9963732 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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