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  1. Article ; Online: Crowd control: Bacillus anthracis and quorum sensing.

    Rollins, Sean M / Schuch, Raymond

    Virulence

    2010  Volume 1, Issue 2, Page(s) 57–59

    Abstract: In 1965, Dr. Alexander Tomasz identified a critical component of the DNA uptake mechanism used by competent Streptococcus pneumoniae: the pneumococci secrete a polypeptide that induces the expression of proteins to allow foreign DNA to pass through the ... ...

    Abstract In 1965, Dr. Alexander Tomasz identified a critical component of the DNA uptake mechanism used by competent Streptococcus pneumoniae: the pneumococci secrete a polypeptide that induces the expression of proteins to allow foreign DNA to pass through the bacterium's cell wall. This hormone-like substance was the first of numerous "quorum-sensing" factors that have since been identified in many microbial processes.  Detailed insights into the molecular mechanisms of quorum-sensing are now emerging, owing largely to studies focusing on the ability of marine organisms like Vibrio fischeri and Vibrio harveyi to produce light at high cell densities.  The complexities of bioluminescence induction, and indeed that of an ever increasing group of other quorum-sensing phenotypes, show that such signaling pathways are not just an interesting phenomenon but rather represent a widespread mechanism by which bacterial populations can communicate, coordinate behavior and act in a cooperative manner in the environment.
    MeSH term(s) Bacillus anthracis/genetics ; Bacillus anthracis/physiology ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Gene Expression Regulation, Bacterial ; Quorum Sensing
    Chemical Substances Bacterial Proteins
    Language English
    Publishing date 2010-03
    Publishing country United States
    Document type Comment ; Editorial
    ZDB-ID 2657572-3
    ISSN 2150-5608 ; 2150-5594
    ISSN (online) 2150-5608
    ISSN 2150-5594
    DOI 10.4161/viru.1.2.11051
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A ridge-to-reef ecosystem microbial census reveals environmental reservoirs for animal and plant microbiomes.

    Amend, Anthony S / Swift, Sean O I / Darcy, John L / Belcaid, Mahdi / Nelson, Craig E / Buchanan, Joshua / Cetraro, Nicolas / Fraiola, Kauaoa M S / Frank, Kiana / Kajihara, Kacie / McDermot, Terrance G / McFall-Ngai, Margaret / Medeiros, Matthew / Mora, Camilo / Nakayama, Kirsten K / Nguyen, Nhu H / Rollins, Randi L / Sadowski, Peter / Sparagon, Wesley /
    Téfit, Mélisandre A / Yew, Joanne Y / Yogi, Danyel / Hynson, Nicole A

    Proceedings of the National Academy of Sciences of the United States of America

    2022  Volume 119, Issue 33, Page(s) e2204146119

    Abstract: Microbes are found in nearly every habitat and organism on the planet, where they are critical to host health, fitness, and metabolism. In most organisms, few microbes are inherited at birth; instead, acquiring microbiomes generally involves complicated ... ...

    Abstract Microbes are found in nearly every habitat and organism on the planet, where they are critical to host health, fitness, and metabolism. In most organisms, few microbes are inherited at birth; instead, acquiring microbiomes generally involves complicated interactions between the environment, hosts, and symbionts. Despite the criticality of microbiome acquisition, we know little about where hosts' microbes reside when not in or on hosts of interest. Because microbes span a continuum ranging from generalists associating with multiple hosts and habitats to specialists with narrower host ranges, identifying potential sources of microbial diversity that can contribute to the microbiomes of unrelated hosts is a gap in our understanding of microbiome assembly. Microbial dispersal attenuates with distance, so identifying sources and sinks requires data from microbiomes that are contemporary and near enough for potential microbial transmission. Here, we characterize microbiomes across adjacent terrestrial and aquatic hosts and habitats throughout an entire watershed, showing that the most species-poor microbiomes are partial subsets of the most species-rich and that microbiomes of plants and animals are nested within those of their environments. Furthermore, we show that the host and habitat range of a microbe within a single ecosystem predicts its global distribution, a relationship with implications for global microbial assembly processes. Thus, the tendency for microbes to occupy multiple habitats and unrelated hosts enables persistent microbiomes, even when host populations are disjunct. Our whole-watershed census demonstrates how a nested distribution of microbes, following the trophic hierarchies of hosts, can shape microbial acquisition.
    MeSH term(s) Animals ; Bacteria ; Ecosystem ; Microbiota ; Plants/microbiology
    Language English
    Publishing date 2022-08-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2204146119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Care Levels for Fetal Therapy Centers.

    Baschat, Ahmet A / Blackwell, Sean B / Chatterjee, Debnath / Cummings, James J / Emery, Stephen P / Hirose, Shinjiro / Hollier, Lisa M / Johnson, Anthony / Kilpatrick, Sarah J / Luks, Francois I / Menard, M Kathryn / McCullough, Lawrence B / Moldenhauer, Julie S / Moon-Grady, Anita J / Mychaliska, George B / Narvey, Michael / Norton, Mary E / Rollins, Mark D / Skarsgard, Eric D /
    Tsao, KuoJen / Warner, Barbara B / Wilpers, Abigail / Ryan, Greg

    Obstetrics and gynecology

    2022  Volume 139, Issue 6, Page(s) 1027–1042

    Abstract: Fetal therapies undertaken to improve fetal outcome or to optimize transition to neonate life often entail some level of maternal, fetal, or neonatal risk. A fetal therapy center needs access to resources to carry out such therapies and to manage ... ...

    Abstract Fetal therapies undertaken to improve fetal outcome or to optimize transition to neonate life often entail some level of maternal, fetal, or neonatal risk. A fetal therapy center needs access to resources to carry out such therapies and to manage maternal, fetal, and neonatal complications that might arise, either related to the therapy per se or as part of the underlying fetal or maternal condition. Accordingly, a fetal therapy center requires a dedicated operational infrastructure and necessary resources to allow for appropriate oversight and monitoring of clinical performance and to facilitate multidisciplinary collaboration between the relevant specialties. Three care levels for fetal therapy centers are proposed to match the anticipated care complexity, with appropriate resources to achieve an optimal outcome at an institutional and regional level. A level I fetal therapy center should be capable of offering fetal interventions that may be associated with obstetric risks of preterm birth or membrane rupture but that would be very unlikely to require maternal medical subspecialty or intensive care, with neonatal risks not exceeding those of moderate prematurity. A level II center should have the incremental capacity to provide maternal intensive care and to manage extreme neonatal prematurity. A level III therapy center should offer the full range of fetal interventions (including open fetal surgery) and be able manage any of the associated maternal complications and comorbidities, as well as have access to neonatal and pediatric surgical intervention including indicated surgery for neonates with congenital anomalies.
    MeSH term(s) Child ; Female ; Fetal Membranes, Premature Rupture ; Fetal Therapies ; Humans ; Infant, Newborn ; Infant, Premature ; Pregnancy ; Premature Birth ; Prenatal Care
    Language English
    Publishing date 2022-05-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 207330-4
    ISSN 1873-233X ; 0029-7844
    ISSN (online) 1873-233X
    ISSN 0029-7844
    DOI 10.1097/AOG.0000000000004793
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Eculizumab for complement mediated thrombotic microangiopathy in sickle cell disease.

    Chonat, Satheesh / Graciaa, Sara / Shin, H Stella / Newton, Joanna G / Quarmyne, Maa-Ohui / Boudreaux, Jeanne / Tang, Amy / Zerra, Patricia E / Rollins, Margo R / Josephson, Cassandra D / Brown, Clark / Joiner, Clinton H / Fasano, Ross M / Stowell, Sean R

    Haematologica

    2020  Volume 105, Issue 12, Page(s) 2887–2891

    MeSH term(s) Anemia, Sickle Cell/complications ; Anemia, Sickle Cell/drug therapy ; Antibodies, Monoclonal, Humanized/therapeutic use ; Complement System Proteins ; Humans ; Thrombotic Microangiopathies/drug therapy ; Thrombotic Microangiopathies/etiology
    Chemical Substances Antibodies, Monoclonal, Humanized ; Complement System Proteins (9007-36-7) ; eculizumab (A3ULP0F556)
    Language English
    Publishing date 2020-12-01
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 2333-4
    ISSN 1592-8721 ; 0017-6567 ; 0390-6078
    ISSN (online) 1592-8721
    ISSN 0017-6567 ; 0390-6078
    DOI 10.3324/haematol.2020.262006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Development of a Culturally Appropriate Smokeless Tobacco Cessation Program for American Indians.

    Rollins, Kathryn / Lewis, Charley / Edward Smith, T / Goeckner, Ryan T / Hale, Jason W / Nazir, Niaman / Faseru, Babalola / Allen Greiner, K / Daley, Sean M / Choi, Won S / Daley, Christine M

    Journal of health disparities research and practice

    2019  Volume 11, Issue 1, Page(s) 45–55

    Abstract: This study describes a multiphasic approach to the development of a smokeless tobacco cessation program targeted for American Indians (AI) of different tribal nations. The authors gathered formative data from a series of focus groups and interviews to ... ...

    Abstract This study describes a multiphasic approach to the development of a smokeless tobacco cessation program targeted for American Indians (AI) of different tribal nations. The authors gathered formative data from a series of focus groups and interviews to investigate the knowledge, attitudes, and beliefs of AI and smokeless tobacco (SLT) use. Predominant themes emerged from four major topic areas (SLT use, initiation and barriers, policy, and program development) across both studies. This study further assessed educational materials developed for the cessation program for scientific accuracy, readability, and cultural appropriateness. Program materials were scientifically accurate and culturally appropriate. The average corrected reading grade level was 6.3 using the Fry formula and 7.1 using the SMOG formula. Based on this research, a detailed approach to formative research can be used in combination with input from community members to develop health interventions that address health disparities for a specific population.
    Language English
    Publishing date 2019-02-15
    Publishing country United States
    Document type Journal Article
    ISSN 2166-5222
    ISSN 2166-5222
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: American Indian Tribal College Student's Knowledge, Attitudes and Beliefs about Recreational and Traditional Tobacco Use.

    Rollins, Kathryn / Jd, Christina M Pacheco / Daley, Sean M / Nazir, Niaman / Lewis, Charley / Choi, Won S / Daley, Christine M

    Juniper online journal of public health

    2017  Volume 2, Issue 1

    Abstract: Introduction: American Indians (AI) have the highest smoking rates of any racial/ethnic group in the U.S., in addition to low success rates of tobacco cessation. The substitution of commercial tobacco for traditional tobacco may have played a role in ... ...

    Abstract Introduction: American Indians (AI) have the highest smoking rates of any racial/ethnic group in the U.S., in addition to low success rates of tobacco cessation. The substitution of commercial tobacco for traditional tobacco may have played a role in the prevalence rates of recreational tobacco use among AI. The present study explored the impact of tribal college students' knowledge, attitudes and beliefs about traditional tobacco use on their recreational cigarette smoking behaviors.
    Methods: Multiple methods were used to recruit participants attending a tribal college. A total of 101 AI tribal college students completed a demographic survey and participated in focus groups or individual interviews assessing traditional and recreational tobacco use.
    Results: AI tribal college student's recreational smoking has an influence on various health behaviors, including poor eating habits, decreased physical activity, and elevated tobacco use in association with alcohol consumption. Differences between the use of and motivation behind smokeless tobacco and cigarette use were seen. In addition, participants reported differences between using tobacco for traditional purposes such as in ceremony or during prayer in comparison to recreational tobacco use. Conclusion: These findings highlight AI students' beliefs about recreational tobacco, smokeless tobacco, and traditional tobacco use. Differences related to behaviors associated with traditional tobacco use have important implications for future cessation efforts for AI smokers.
    Language English
    Publishing date 2017-05-26
    Publishing country United States
    Document type Journal Article
    ISSN 2573-2153
    ISSN (online) 2573-2153
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: An Assay to Study Intra-Chromosomal Deletions in Yeast.

    Lucas, Bailey E / McPherson, Matthew T / Hawk, Tila M / Wilson, Lexia N / Kroh, Jacob M / Hickman, Kyle G / Fitzgerald, Sean R / Disbennett, W Miguel / Rollins, P Daniel / Hylton, Hannah M / Baseer, Mohammed A / Montgomery, Paige N / Wu, Jian-Qiu / Petreaca, Ruben C

    Methods and protocols

    2019  Volume 2, Issue 3

    Abstract: An accurate DNA damage response pathway is critical for the repair of DNA double-strand breaks. Repair may occur by homologous recombination, of which many different sub-pathways have been identified. Some recombination pathways are conservative, meaning ...

    Abstract An accurate DNA damage response pathway is critical for the repair of DNA double-strand breaks. Repair may occur by homologous recombination, of which many different sub-pathways have been identified. Some recombination pathways are conservative, meaning that the chromosome sequences are preserved, and others are non-conservative, leading to some alteration of the DNA sequence. We describe an in vivo genetic assay to study non-conservative intra-chromosomal deletions at regions of non-tandem direct repeats in
    Language English
    Publishing date 2019-08-26
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2409-9279
    ISSN (online) 2409-9279
    DOI 10.3390/mps2030074
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Challenges in heart transplantation during COVID-19: A single-center experience.

    Singhvi, Aditi / Barghash, Maya / Lala, Anuradha / Mitter, Sumeet S / Parikh, Aditya / Oliveros, Estefania / Rollins, Brett M / Brunjes, Danielle L / Alvarez-Garcia, Jesus / Johnston, Erika / Ryan, Kieran / Itagaki, Shinobu / Moss, Noah / Pinney, Sean P / Anyanwu, Anelechi / Mancini, Donna

    The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation

    2020  Volume 39, Issue 9, Page(s) 894–903

    Abstract: Background: Orthotopic heart transplantation (OHT) recipients may be particularly vulnerable to coronavirus disease 2019 (COVID-19). OHT during the pandemic presents unique challenges in terms of feasibility and safety.: Methods: Chart review was ... ...

    Abstract Background: Orthotopic heart transplantation (OHT) recipients may be particularly vulnerable to coronavirus disease 2019 (COVID-19). OHT during the pandemic presents unique challenges in terms of feasibility and safety.
    Methods: Chart review was performed for consecutive OHT recipients with COVID-19 and waitlisted patients who underwent OHT from March 1, 2020 to May 15, 2020.
    Results: Of the approximately 400 OHT recipients followed at our institution, 22 acquired COVID-19. Clinical characteristics included median age 59 (range, 49-71) years, 14 (63.6%) were male, and median time from OHT to infection was 4.6 (2.5-20.6) years. Symptoms included fever (68.2%), gastrointestinal complaints (55%), and cough (46%). COVID-19 was severe or critical in 5 (23%). All patients had elevated inflammatory biomarkers. Immunosuppression was modified in 85% of patients. Most (n = 16, 86.4%) were hospitalized, 18% required intubation, and 14% required vasopressor support. Five patients (23%) expired. None of the patients requiring intubation survived. Five patients underwent OHT during the pandemic. They were all males, ranging from 30 to 59 years of age. Two were transplanted at United Network of Organ Sharing Status 1 or 2, 1 at Status 3, and 2 at Status 4. All were successfully discharged and are alive without allograft dysfunction or rejection. One contracted mild COVID-19 after the index hospitalization.
    Conclusion: OHT recipients with COVID-19 appear to have outcomes similar to the general population hospitalized with COVID-19. OHT during the pandemic is feasible when appropriate precautions are taken. Further study is needed to guide immunosuppression management in OHT recipients affected by COVID-19.
    MeSH term(s) Aged ; Betacoronavirus ; COVID-19 ; Coronavirus Infections/complications ; Coronavirus Infections/epidemiology ; Feasibility Studies ; Female ; Graft Rejection/prevention & control ; Heart Failure/complications ; Heart Failure/surgery ; Heart Transplantation/methods ; Humans ; Immunosuppression Therapy/methods ; Immunosuppressive Agents/therapeutic use ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/complications ; Pneumonia, Viral/epidemiology ; Retrospective Studies ; Risk Factors ; SARS-CoV-2 ; Transplantation, Homologous ; Treatment Outcome
    Chemical Substances Immunosuppressive Agents
    Keywords covid19
    Language English
    Publishing date 2020-06-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1062522-7
    ISSN 1557-3117 ; 1053-2498
    ISSN (online) 1557-3117
    ISSN 1053-2498
    DOI 10.1016/j.healun.2020.06.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Challenges in heart transplantation during COVID-19

    Singhvi, Aditi / Barghash, Maya / Lala, Anuradha / Mitter, Sumeet S. / Parikh, Aditya / Oliveros, Estefania / Rollins, Brett M. / Brunjes, Danielle L. / Alvarez-Garcia, Jesus / Johnston, Erika / Ryan, Kieran / Itagaki, Shinobu / Moss, Noah / Pinney, Sean P. / Anyanwu, Anelechi / Mancini, Donna

    The Journal of Heart and Lung Transplantation

    A single-center experience

    2020  Volume 39, Issue 9, Page(s) 894–903

    Keywords Surgery ; Pulmonary and Respiratory Medicine ; Transplantation ; Cardiology and Cardiovascular Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 1062522-7
    ISSN 1557-3117 ; 1053-2498
    ISSN (online) 1557-3117
    ISSN 1053-2498
    DOI 10.1016/j.healun.2020.06.015
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: A normative spatiotemporal MRI atlas of the fetal brain for automatic segmentation and analysis of early brain growth.

    Gholipour, Ali / Rollins, Caitlin K / Velasco-Annis, Clemente / Ouaalam, Abdelhakim / Akhondi-Asl, Alireza / Afacan, Onur / Ortinau, Cynthia M / Clancy, Sean / Limperopoulos, Catherine / Yang, Edward / Estroff, Judy A / Warfield, Simon K

    Scientific reports

    2017  Volume 7, Issue 1, Page(s) 476

    Abstract: Longitudinal characterization of early brain growth in-utero has been limited by a number of challenges in fetal imaging, the rapid change in size, shape and volume of the developing brain, and the consequent lack of suitable algorithms for fetal brain ... ...

    Abstract Longitudinal characterization of early brain growth in-utero has been limited by a number of challenges in fetal imaging, the rapid change in size, shape and volume of the developing brain, and the consequent lack of suitable algorithms for fetal brain image analysis. There is a need for an improved digital brain atlas of the spatiotemporal maturation of the fetal brain extending over the key developmental periods. We have developed an algorithm for construction of an unbiased four-dimensional atlas of the developing fetal brain by integrating symmetric diffeomorphic deformable registration in space with kernel regression in age. We applied this new algorithm to construct a spatiotemporal atlas from MRI of 81 normal fetuses scanned between 19 and 39 weeks of gestation and labeled the structures of the developing brain. We evaluated the use of this atlas and additional individual fetal brain MRI atlases for completely automatic multi-atlas segmentation of fetal brain MRI. The atlas is available online as a reference for anatomy and for registration and segmentation, to aid in connectivity analysis, and for groupwise and longitudinal analysis of early brain growth.
    MeSH term(s) Algorithms ; Brain/anatomy & histology ; Brain/diagnostic imaging ; Brain/growth & development ; Fetal Development ; Fetus/anatomy & histology ; Humans ; Image Interpretation, Computer-Assisted ; Image Processing, Computer-Assisted ; Magnetic Resonance Imaging/methods ; Spatio-Temporal Analysis
    Language English
    Publishing date 2017-03-28
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-00525-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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