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  1. Article ; Online: A Simian Immunodeficiency Virus-Infected Macaque Model of

    Singh, Inderpal / Tzipori, Saul

    AIDS research and human retroviruses

    2023  Volume 39, Issue 2, Page(s) 76–83

    Abstract: Microsporidiosis caused ... ...

    Abstract Microsporidiosis caused by
    MeSH term(s) Animals ; Simian Immunodeficiency Virus ; Enterocytozoon ; Simian Acquired Immunodeficiency Syndrome/complications ; Macaca mulatta ; HIV Infections ; Microsporidiosis/pathology ; CD4-Positive T-Lymphocytes
    Language English
    Publishing date 2023-01-25
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 639130-8
    ISSN 1931-8405 ; 0889-2229
    ISSN (online) 1931-8405
    ISSN 0889-2229
    DOI 10.1089/AID.2022.0091
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1928: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: A highly antigenic fragment within the zoonotic Cryptosporidium parvum Gp900 glycoprotein (Domain 3) is absent in human restricted Cryptosporidium species.

    Dayao, Denise Ann E / Jaskiewicz, Justyna J / Sheoran, Abhineet S / Widmer, Giovanni / Tzipori, Saul

    PloS one

    2023  Volume 18, Issue 8, Page(s) e0287997

    Abstract: We identified a fragment (Domain 3-D3) of the immunodominant sporozoite surface glycoprotein of the zoonotic parasite Cryptosporidium gp900, which is absent C. hominis and C. parvum anthroponosum. The fragment is highly antigenic and is able to ... ...

    Abstract We identified a fragment (Domain 3-D3) of the immunodominant sporozoite surface glycoprotein of the zoonotic parasite Cryptosporidium gp900, which is absent C. hominis and C. parvum anthroponosum. The fragment is highly antigenic and is able to effectively differentiate between zoonotic C. parvum and species/genotypes that infect preferentially humans. D3 detection provides a serological tool to determine whether the source of human cryptosporidiosis is of animal or human origin. We demonstrate this in experimentally challenged piglets, mice, rats, and alpaca. We speculate that the absence of this fragment from the C. hominis and C. parvum anthroponosum gp900 protein may play a key role in their host restriction.
    MeSH term(s) Humans ; Animals ; Mice ; Rats ; Swine ; Cryptosporidium parvum ; Cryptosporidium ; Cryptosporidiosis ; Glycoproteins ; Membrane Glycoproteins ; Camelids, New World ; Propionibacterium acnes
    Chemical Substances Glycoproteins ; Membrane Glycoproteins
    Language English
    Publishing date 2023-08-17
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0287997
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  3. Article ; Online: Identification and characterization of a new 34 kDa MORN motif-containing sporozoite surface-exposed protein, Cp-P34, unique to Cryptosporidium.

    Jaskiewicz, Justyna J / Tremblay, Jacqueline M / Tzipori, Saul / Shoemaker, Charles B

    International journal for parasitology

    2021  Volume 51, Issue 9, Page(s) 761–775

    Abstract: ... on critical externally exposed virulence factors expressed in the parasite s invasive stages. However, no ... Nexus (MORN) repeats, is found in excysted sporozoites as well as in the parasite s intracellular stages ...

    Abstract Despite the public health impact of childhood diarrhea caused by Cryptosporidium, effective drugs and vaccines against this parasite are unavailable. Efforts to identify vaccine targets have focused on critical externally exposed virulence factors expressed in the parasite s invasive stages. However, no single surface antigen has yet been found that can elicit a significant protective immune response and it is likely that pooling multiple immune targets will be necessary. Discovery of surface proteins on Cryptosporidium sporozoites is therefore vital to this effort to develop a multi-antigenic vaccine. In this study we applied a novel single-domain camelid antibody (VHH) selection method to identify immunogenic proteins expressed on the surface of Cryptosporidium parvum sporozoites. By this approach, VHHs were identified that recognize two sporozoite surface-exposed antigens, the previously identified gp900 and an unrecognized immunogenic protein, Cp-P34. This Cp-P34 antigen, which contains multiple Membrane Occupation and Recognition Nexus (MORN) repeats, is found in excysted sporozoites as well as in the parasite s intracellular stages. Cp-P34 appears to accumulate inside the parasite and transiently appears on the surface of sporozoites to be shed in trails. Identical or nearly identical orthologs of Cp-P34 are found in the Cryptosporidium hominis and Cryptosporidium tyzzeri genomes. Except for the conserved MORN motifs, the Cp-P34 gene shares no significant homology with genes of other protozoans and thus appears to be unique to Cryptosporidium spp. Cp-P34 elicits immune responses in naturally exposed alpacas and warrants further investigation as a potential vaccine candidate.
    MeSH term(s) Animals ; Cryptosporidiosis ; Cryptosporidium parvum/genetics ; Membrane Proteins/genetics ; Protozoan Proteins/genetics ; Sporozoites
    Chemical Substances Membrane Proteins ; Protozoan Proteins
    Language English
    Publishing date 2021-03-25
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 120518-3
    ISSN 1879-0135 ; 0020-7519
    ISSN (online) 1879-0135
    ISSN 0020-7519
    DOI 10.1016/j.ijpara.2021.01.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 789: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Cryptosporidium

    Greigert, Valentin / Saraav, Iti / Son, Juhee / Zhu, Yinxing / Dayao, Denise / Antia, Avan / Tzipori, Saul / Witola, William H / Stappenbeck, Thaddeus S / Ding, Siyuan / Sibley, L David

    Gut microbes

    2024  Volume 16, Issue 1, Page(s) 2297897

    Abstract: Cryptosporidiosis is a major cause of severe diarrheal disease in infants from resource poor settings. The majority of infections are caused by the human-specific ... ...

    Abstract Cryptosporidiosis is a major cause of severe diarrheal disease in infants from resource poor settings. The majority of infections are caused by the human-specific pathogen
    MeSH term(s) Infant ; Humans ; Cryptosporidiosis ; Interferon Lambda ; Rotavirus ; Cryptosporidium ; Gastrointestinal Microbiome ; Epithelial Cells ; Zea mays
    Chemical Substances Interferon Lambda
    Language English
    Publishing date 2024-01-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2575755-6
    ISSN 1949-0984 ; 1949-0984
    ISSN (online) 1949-0984
    ISSN 1949-0984
    DOI 10.1080/19490976.2023.2297897
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Scalable cryopreservation of infectious Cryptosporidium hominis oocysts by vitrification.

    Jaskiewicz, Justyna J / Dayao, Denise Ann E / Girouard, Donald / Sevenler, Derin / Widmer, Giovanni / Toner, Mehmet / Tzipori, Saul / Sandlin, Rebecca D

    PLoS pathogens

    2023  Volume 19, Issue 6, Page(s) e1011425

    Abstract: Cryptosporidium hominis is a serious cause of childhood diarrhea in developing countries. The development of therapeutics is impeded by major technical roadblocks including lack of cryopreservation and simple culturing methods. This impacts the ... ...

    Abstract Cryptosporidium hominis is a serious cause of childhood diarrhea in developing countries. The development of therapeutics is impeded by major technical roadblocks including lack of cryopreservation and simple culturing methods. This impacts the availability of optimized/standardized singular sources of infectious parasite oocysts for research and human challenge studies. The human C. hominis TU502 isolate is currently propagated in gnotobiotic piglets in only one laboratory, which limits access to oocysts. Streamlined cryopreservation could enable creation of a biobank to serve as an oocyst source for research and distribution to other investigators requiring C. hominis. Here, we report cryopreservation of C. hominis TU502 oocysts by vitrification using specially designed specimen containers scaled to 100 μL volume. Thawed oocysts exhibit ~70% viability with robust excystation and 100% infection rate in gnotobiotic piglets. The availability of optimized/standardized sources of oocysts may streamline drug and vaccine evaluation by enabling wider access to biological specimens.
    MeSH term(s) Animals ; Humans ; Swine ; Cryptosporidium ; Cryptosporidiosis/parasitology ; Vitrification ; Oocysts ; Cryopreservation ; Cryptosporidium parvum
    Language English
    Publishing date 2023-06-08
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1011425
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  6. Article ; Online: Co-administration of an effector antibody enhances the half-life and therapeutic potential of RNA-encoded nanobodies.

    Thran, Moritz / Pönisch, Marion / Danz, Hillary / Horscroft, Nigel / Ichtchenko, Konstantin / Tzipori, Saul / Shoemaker, Charles B

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 14632

    Abstract: The incidence of Clostridioides difficile infection (CDI) and associated mortality have increased rapidly worldwide in recent years. Therefore, it is critical to develop new therapies for CDI. Here we report on the development of mRNA-LNPs encoding ... ...

    Abstract The incidence of Clostridioides difficile infection (CDI) and associated mortality have increased rapidly worldwide in recent years. Therefore, it is critical to develop new therapies for CDI. Here we report on the development of mRNA-LNPs encoding camelid-derived V
    MeSH term(s) Swine ; Animals ; Mice ; Single-Domain Antibodies ; RNA ; Clostridioides difficile ; Half-Life ; Antibodies ; RNA, Messenger
    Chemical Substances Single-Domain Antibodies ; RNA (63231-63-0) ; Antibodies ; RNA, Messenger
    Language English
    Publishing date 2023-09-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-41092-7
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  7. Article: Cryptosporidium

    Greigert, Valentin / Saraav, Iti / Son, Juhee / Dayao, Denise / Antia, Avan / Tzipori, Saul / Witola, William H / Stappenbeck, Thaddeus S / Ding, Siyuan / Sibley, L David

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Cryptosporidiosis is a major cause of severe diarrheal disease in infants from resource poor settings. The majority of infections are caused by the human-specific ... ...

    Abstract Cryptosporidiosis is a major cause of severe diarrheal disease in infants from resource poor settings. The majority of infections are caused by the human-specific pathogen
    Language English
    Publishing date 2023-09-06
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.08.30.555581
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The piglet acute diarrhea model for evaluating efficacy of treatment and control of cryptosporidiosis.

    Lee, Sangun / Beamer, Gillian / Tzipori, Saul

    Human vaccines & immunotherapeutics

    2018  Volume 15, Issue 6, Page(s) 1445–1452

    Abstract: ... ...

    Abstract Cryptosporidium
    MeSH term(s) Age Factors ; Animals ; Cryptosporidiosis/immunology ; Cryptosporidiosis/prevention & control ; Cryptosporidium ; Diarrhea/microbiology ; Disease Models, Animal ; Feces/microbiology ; Germ-Free Life ; Humans ; Swine
    Language English
    Publishing date 2018-08-27
    Publishing country United States
    Document type Evaluation Study ; Journal Article
    ZDB-ID 2664176-8
    ISSN 2164-554X ; 2164-5515
    ISSN (online) 2164-554X
    ISSN 2164-5515
    DOI 10.1080/21645515.2018.1498436
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6967: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  9. Article: A Multi-Specific DARPin Potently Neutralizes Shiga Toxin 2 via Simultaneous Modulation of Both Toxin Subunits.

    Zeng, Yu / Jiang, Mengqiu / Robinson, Sally / Peng, Zeyu / Chonira, Vikas / Simeon, Rudo / Tzipori, Saul / Zhang, Junjie / Chen, Zhilei

    Bioengineering (Basel, Switzerland)

    2022  Volume 9, Issue 10

    Abstract: Shiga toxin- ... ...

    Abstract Shiga toxin-producing
    Language English
    Publishing date 2022-09-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2746191-9
    ISSN 2306-5354
    ISSN 2306-5354
    DOI 10.3390/bioengineering9100511
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  10. Article ; Online: Pregnant sows immunized with Cryptosporidium parvum significantly reduced infection in newborn piglets challenged with C. parvum but not with C. hominis.

    Sheoran, Abhineet / Carvalho, Alison / Mimbela, Ruby Pina / South, Adam / Major, Samuel / Ginese, Melanie / Girouard, Donald / Tzipori, Saul

    PLoS neglected tropical diseases

    2022  Volume 16, Issue 7, Page(s) e0010690

    Abstract: Background: The piglet is the only model to investigate the immunogenic relationship between Cryptosporidium hominis and C. parvum, the species responsible for diarrhea in humans. Despite being indistinguishable antigenically, and high genetic homology ... ...

    Abstract Background: The piglet is the only model to investigate the immunogenic relationship between Cryptosporidium hominis and C. parvum, the species responsible for diarrhea in humans. Despite being indistinguishable antigenically, and high genetic homology between them, they are only moderately cross protective after an active infection.
    Methodology/principal findings: Here we examined the degree of passive protection conferred to piglets suckling sows immunized during pregnancy with C. parvum. After birth suckling piglets were challenged orally with either C. parvum or C. hominis at age 5 days. Animals challenged with C. parvum had significant reduction of infection rate, while piglets challenged with C. hominis showed no reduction despite high C. parvum serum and colostrum IgG and IgA antibody.
    Conclusions/significance: We add these data to earlier studies where we described that infection derived immunity provides partial cross-protection. Together, it appears that for full protection, vaccines against human cryptosporidiosis must contain antigenic elements derived from both species.
    MeSH term(s) Animals ; Animals, Newborn ; Child, Preschool ; Colostrum ; Cryptosporidiosis/prevention & control ; Cryptosporidium/genetics ; Cryptosporidium parvum ; Female ; Humans ; Pregnancy ; Swine
    Language English
    Publishing date 2022-07-29
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2735
    ISSN (online) 1935-2735
    ISSN 1935-2735
    DOI 10.1371/journal.pntd.0010690
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