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  1. Article ; Online: Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids

    Yunjun Pan / Guobang Li / Ruxin Liu / Jiawei Guo / Yunjie Liu / Mingyu Liu / Xingwang Zhang / Luping Chi / Kangwei Xu / Ruibo Wu / Yuzhong Zhang / Yuezhong Li / Xiang Gao / Shengying Li

    Nature Communications, Vol 14, Iss 1, Pp 1-

    2023  Volume 14

    Abstract: The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant ... ...

    Abstract The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant mechanistic insights.
    Keywords Science ; Q
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Multilevel design and construction in nanomembrane rolling for three-dimensional angle-sensitive photodetection.

    Zhang, Ziyu / Wu, Binmin / Wang, Yang / Cai, Tianjun / Ma, Mingze / You, Chunyu / Liu, Chang / Jiang, Guobang / Hu, Yuhang / Li, Xing / Chen, Xiang-Zhong / Song, Enming / Cui, Jizhai / Huang, Gaoshan / Kiravittaya, Suwit / Mei, Yongfeng

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 3066

    Abstract: Releasing pre-strained two-dimensional nanomembranes to assemble on-chip three-dimensional devices is crucial for upcoming advanced electronic and optoelectronic applications. However, the release process is affected by many unclear factors, hindering ... ...

    Abstract Releasing pre-strained two-dimensional nanomembranes to assemble on-chip three-dimensional devices is crucial for upcoming advanced electronic and optoelectronic applications. However, the release process is affected by many unclear factors, hindering the transition from laboratory to industrial applications. Here, we propose a quasistatic multilevel finite element modeling to assemble three-dimensional structures from two-dimensional nanomembranes and offer verification results by various bilayer nanomembranes. Take Si/Cr nanomembrane as an example, we confirm that the three-dimensional structural formation is governed by both the minimum energy state and the geometric constraints imposed by the edges of the sacrificial layer. Large-scale, high-yield fabrication of three-dimensional structures is achieved, and two distinct three-dimensional structures are assembled from the same precursor. Six types of three-dimensional Si/Cr photodetectors are then prepared to resolve the incident angle of light with a deep neural network model, opening up possibilities for the design and manufacturing methods of More-than-Moore-era devices.
    Language English
    Publishing date 2024-04-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-47405-2
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  3. Article ; Online: Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids.

    Pan, Yunjun / Li, Guobang / Liu, Ruxin / Guo, Jiawei / Liu, Yunjie / Liu, Mingyu / Zhang, Xingwang / Chi, Luping / Xu, Kangwei / Wu, Ruibo / Zhang, Yuzhong / Li, Yuezhong / Gao, Xiang / Li, Shengying

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 1669

    Abstract: Cytochrome P450 enzymes play important roles in the biosynthesis of macrolide antibiotics by mediating a vast variety of regio- and stereoselective oxidative modifications, thus improving their chemical diversity, biological activities, and ... ...

    Abstract Cytochrome P450 enzymes play important roles in the biosynthesis of macrolide antibiotics by mediating a vast variety of regio- and stereoselective oxidative modifications, thus improving their chemical diversity, biological activities, and pharmaceutical properties. Tremendous efforts have been made on engineering the reactivity and selectivity of these useful biocatalysts. However, the 20 proteinogenic amino acids cannot always satisfy the requirement of site-directed/random mutagenesis and rational protein design of P450 enzymes. To address this issue, herein, we practice the semi-rational non-canonical amino acid mutagenesis for the pikromycin biosynthetic P450 enzyme PikC, which recognizes its native macrolide substrates with a 12- or 14-membered ring macrolactone linked to a deoxyamino sugar through a unique sugar-anchoring mechanism. Based on a semi-rationally designed substrate binding strategy, non-canonical amino acid mutagenesis at the His238 position enables the unnatural activities of several PikC mutants towards the macrolactone precursors without any sugar appendix. With the aglycone hydroxylating activities, the pikromycin biosynthetic pathway is rewired by the representative mutant PikC
    MeSH term(s) Amino Acids/genetics ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism ; Macrolides/chemistry ; Mutagenesis, Site-Directed ; Anti-Bacterial Agents ; Substrate Specificity
    Chemical Substances picromycin (FBM8G3Z439) ; Amino Acids ; Cytochrome P-450 Enzyme System (9035-51-2) ; Macrolides ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-03-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-37288-0
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  4. Article ; Online: Cerium-doped bimetal organic framework as a superhigh capacity cathode for rechargeable alkaline batteries.

    Li, Junpeng / Zhao, Guobang / Zhao, Hongyang / Zhao, Ningning / Lu, Leilei / Liu, Nailiang / Wang, Miao / Ma, Chunjie / Zhang, Qian / Du, Yaping

    Nanoscale

    2021  Volume 13, Issue 6, Page(s) 3581–3587

    Abstract: In this work, cerium (Ce)-doped NiCo-MOF (metal organic framework) was investigated for its application as a cathode material of alkaline batteries. Inert substitution of Ni/Co by Ce in MOF can make Ce to become part of the backbone of the framework and ... ...

    Abstract In this work, cerium (Ce)-doped NiCo-MOF (metal organic framework) was investigated for its application as a cathode material of alkaline batteries. Inert substitution of Ni/Co by Ce in MOF can make Ce to become part of the backbone of the framework and then ensure structure stability during redox reaction, which greatly improved charge and discharge cycle stability. With dopant mole fraction up to 5%, the redox potential of NiCo-MOF increased by 85%. Adequate Ce doping can potentially enhance rate capacity dramatically due to the large ion radius that provided an extra space for electrolyte ion shutting channel. 1% Ce-doped NiCo-MOF, having a capacity of 286 mA h g
    Language English
    Publishing date 2021-02-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2515664-0
    ISSN 2040-3372 ; 2040-3364
    ISSN (online) 2040-3372
    ISSN 2040-3364
    DOI 10.1039/d0nr08696g
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: AB loop engineered ferritin nanocages for drug loading under benign experimental conditions

    Wang, Wenming / Li, Guobang / Wang, Hongfei / Wang, Lele / Zhao, Guanghua / Zhao, Xuan

    Chemical communications. 2019 Oct. 10, v. 55, no. 82

    2019  

    Abstract: Human ferritin has been explored as a potential drug nanocarrier, but extreme conditions (pH ≤ 2.0) are required for the encapsulation of drugs. Here, by engineering the AB loop of ferritin, we obtained a new ferritin variant with no new pores, which can ...

    Abstract Human ferritin has been explored as a potential drug nanocarrier, but extreme conditions (pH ≤ 2.0) are required for the encapsulation of drugs. Here, by engineering the AB loop of ferritin, we obtained a new ferritin variant with no new pores, which can disassemble at pH 3.0 or 4.0 and reassemble at pH 7.0. Consequently, under mild conditions, drugs can be encapsulated within this new ferritin nanocage.
    Keywords chemical compounds ; chemical reactions ; drugs ; encapsulation ; engineering ; ferritin ; humans ; nanocarriers ; pH
    Language English
    Dates of publication 2019-1010
    Size p. 12344-12347.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/c9cc05247j
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  6. Article: Comprehensive Insights into the Catalytic Mechanism of Middle East Respiratory Syndrome 3C-Like Protease and Severe Acute Respiratory Syndrome 3C-Like Protease.

    Wang, Hao / He, Shuai / Deng, Weilong / Zhang, Ying / Li, Guobang / Sun, Jixue / Zhao, Wei / Guo, Yu / Yin, Zheng / Li, Dongmei / Shang, Luqing

    ACS catalysis

    2020  Volume 10, Page(s) 5871–5890

    Abstract: Coronavirus 3C-like protease ( ... ...

    Abstract Coronavirus 3C-like protease (3CL
    Keywords covid19
    Language English
    Publishing date 2020-04-28
    Publishing country United States
    Document type Journal Article
    ISSN 2155-5435
    ISSN 2155-5435
    DOI 10.1021/acscatal.0c00110
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  7. Article ; Online: AB loop engineered ferritin nanocages for drug loading under benign experimental conditions.

    Wang, Wenming / Wang, Lele / Li, Guobang / Zhao, Guanghua / Zhao, Xuan / Wang, Hongfei

    Chemical communications (Cambridge, England)

    2019  Volume 55, Issue 82, Page(s) 12344–12347

    Abstract: Human ferritin has been explored as a potential drug nanocarrier, but extreme conditions (pH ≤ 2.0) are required for the encapsulation of drugs. Here, by engineering the AB loop of ferritin, we obtained a new ferritin variant with no new pores, which can ...

    Abstract Human ferritin has been explored as a potential drug nanocarrier, but extreme conditions (pH ≤ 2.0) are required for the encapsulation of drugs. Here, by engineering the AB loop of ferritin, we obtained a new ferritin variant with no new pores, which can disassemble at pH 3.0 or 4.0 and reassemble at pH 7.0. Consequently, under mild conditions, drugs can be encapsulated within this new ferritin nanocage.
    MeSH term(s) Antibiotics, Antineoplastic/chemistry ; Antibiotics, Antineoplastic/pharmacology ; Cell Proliferation/drug effects ; Doxorubicin/chemistry ; Doxorubicin/pharmacology ; Drug Carriers/chemistry ; Ferritins/chemistry ; Humans ; Hydrogen-Ion Concentration ; Models, Molecular ; Nanoparticles/chemistry ; Protein Engineering ; Protein Structure, Secondary
    Chemical Substances Antibiotics, Antineoplastic ; Drug Carriers ; Doxorubicin (80168379AG) ; Ferritins (9007-73-2)
    Language English
    Publishing date 2019-10-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/c9cc05247j
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  8. Article: Pollen assemblages and anthropogenic influences in the central and western regions of Yunnan Province

    Li, Yumei / Zhang, Yun / Tong, Guobang / Li, Chunhai / Zhao, Long / Kong, Zhaochen

    Science of the total environment. 2019 Jan. 01, v. 646

    2019  

    Abstract: The goal of this study was to provide information on the representation of pollen taxa and the human impact on vegetation by exploring the relationship between modern pollen and vegetation in the central and western regions of Yunnan Province of China. A ...

    Abstract The goal of this study was to provide information on the representation of pollen taxa and the human impact on vegetation by exploring the relationship between modern pollen and vegetation in the central and western regions of Yunnan Province of China. A total of 120 modern pollen samples were collected throughout the study area. The surface pollen assemblage was divided into eight pollen zones (Zones I–VIII) based on vegetation types. We analyzed the palynology and determined the hemeroby levels of each zone in the area. Our results showed that human activity is one of the most important factors that can result in the damage of natural vegetation and increase anthropochory and secondary plants. The hemeroby levels of the surface pollen samples were mainly euhemerobic except for Zones I, II, and VI, which indicates a strong and continuous anthropogenic influence in the central and western regions of Yunnan Province in recent decades. The lowest hemeroby values were observed in Zone I samples, which were collected from Mengla and Jinghong. These areas are in the Xishuangbanna region, which is the most biodiverse region in China and has been well preserved since 1959. Therefore, the extent of human influence of a region can be estimated from the degree of hemeroby established from pollen data, which can be used in the future for nature conservation.
    Keywords anthropogenic activities ; natural resources conservation ; palynology ; pollen ; vegetation types ; China
    Language English
    Dates of publication 2019-0101
    Size p. 368-376.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2018.07.222
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  9. Article ; Online: Crystal structure of the African swine fever virus structural protein p35 reveals its role for core shell assembly.

    Li, Guobang / Fu, Dan / Zhang, Guangshun / Zhao, Dongming / Li, Mingyu / Geng, Xue / Sun, Dongdong / Wang, Yuhui / Chen, Cheng / Jiao, Peng / Cao, Lin / Guo, Yu / Rao, Zihe

    Protein & cell

    2020  Volume 11, Issue 8, Page(s) 600–605

    MeSH term(s) African Swine Fever Virus/chemistry ; African Swine Fever Virus/metabolism ; Animals ; Crystallography, X-Ray ; Models, Molecular ; Protein Conformation ; Swine ; Viral Core Proteins/chemistry ; Viral Core Proteins/metabolism
    Chemical Substances Viral Core Proteins
    Language English
    Publishing date 2020-06-09
    Publishing country Germany
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 2543451-2
    ISSN 1674-8018 ; 1674-800X
    ISSN (online) 1674-8018
    ISSN 1674-800X
    DOI 10.1007/s13238-020-00730-w
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  10. Article ; Online: Distinct Mechanism for the Formation of the Ribonucleoprotein Complex of Tomato Spotted Wilt Virus.

    Guo, Yu / Liu, Baocheng / Ding, Zhenzhen / Li, Guobang / Liu, Meizi / Zhu, Dantong / Sun, Yuna / Dong, Shishang / Lou, Zhiyong

    Journal of virology

    2017  Volume 91, Issue 23

    Abstract: ... ...

    Abstract The
    Language English
    Publishing date 2017-12-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.00892-17
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    Zs.A 609: Show issues Location:
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