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Article ; Online: Lactobacillus for ribosome peptide editing cancer.

Yue, Shousong / He, Qiuxia / Picimbon, Jean-François

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

2023 Volume 25, Issue 6, Page(s) 1522–1544

Abstract: This study reviews newly discovered insect peptide point mutations as new possible cancer research targets. To interpret newly discovered peptide point mutations in insects as new possible cancer research targets, we focused on the numerous peptide ... ...

Abstract	This study reviews newly discovered insect peptide point mutations as new possible cancer research targets. To interpret newly discovered peptide point mutations in insects as new possible cancer research targets, we focused on the numerous peptide changes found in the 'CSP' family on the sex pheromone gland of the female silkworm moth Bombyx mori. We predict that the Bombyx peptide modifications will have a significant effect on cancer CUP (cancers of unknown primary) therapy and that bacterial peptide editing techniques, specifically Lactobacillus combined to CRISPR, will be used to regulate ribosomes and treat cancer in humans.
MeSH term(s)	Animals ; Female ; Humans ; Bombyx/genetics ; Peptides ; Ribosomes/genetics ; Neoplasms/genetics ; Neoplasms/therapy
Chemical Substances	Peptides
Language	English
Publishing date	2023-01-24
Publishing country	Italy
Document type	Journal Article ; Review
ZDB-ID	2397359-6
ISSN	1699-3055 ; 1699-048X
ISSN (online)	1699-3055
ISSN	1699-048X
DOI	10.1007/s12094-022-03066-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Arbuscular mycorrhizal fungi increased peanut (

Kang, Jia / Yang, Wenlong / Liu, Shangwu / Xuan, Ning / Shao, Yahui / Geng, Yun / Afzal, Muhammad / Zhang, Yingxin / Yue, Shousong / Mushtaq, Rubina / Chen, Gao

Frontiers in microbiology

2023 Volume 14, Page(s) 1303979

Abstract: Arbuscular mycorrhizal fungi (AMF) have demonstrated the potential to enhance the saline-alkali tolerance in plants. Nevertheless, the extent to which AMF can ameliorate the tolerance of salt-sensitive plants to alkaline conditions necessitates further ... ...

Abstract	Arbuscular mycorrhizal fungi (AMF) have demonstrated the potential to enhance the saline-alkali tolerance in plants. Nevertheless, the extent to which AMF can ameliorate the tolerance of salt-sensitive plants to alkaline conditions necessitates further investigation. The current study is primarily centered on elucidating the impact of AMF on the growth of the Huayu22 (H22) when cultivated in saline-alkaline soil. We leveraged DNA of rhizosphere microorganisms extracted from saline-alkali soil subjected to AMF treatment and conducted high-throughput sequencing encompassing 16S rRNA gene and ITS sequencing. Our findings from high-throughput sequencing unveiled Proteobacteria and
Language	English
Publishing date	2023-12-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1303979
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Article ; Online: Curing piglets from diarrhea and preparation of a healthy microbiome with Bacillus treatment for industrial animal breeding

Shousong Yue / Zhentian Li / Fuli Hu / Jean-François Picimbon

Scientific Reports, Vol 10, Iss 1, Pp 1-

2020 Volume 18

Abstract: Abstract High-throughput farming of animals for an essential purpose such as large scale health and production of hogs is a challenge for the food industry in the modern world. The problem is that the breeding of livestock for fast growth or high yields ... ...

Abstract	Abstract High-throughput farming of animals for an essential purpose such as large scale health and production of hogs is a challenge for the food industry in the modern world. The problem is that the breeding of livestock for fast growth or high yields of meat is often associated with illness and microbial infection that develop under the breeding conditions. Piglet diarrhea is most common pig disease, leading to heavy mortality and thereby economic loss. We proved that chemical drugs can relieve the symptoms of diarrhea in ill piglets, but they do not treat the underlying cause, i.e. significantly altered bacterial gut flora. Using Illumina sequencing of fecal DNA, we showed that the bacterial gut flora of piglets treated with antibiotics remain close to the ill conditions. However, using Illumina sequencing of fecal DNA from piglets treated with a specific Bacillus (Bacillus subtilis Y-15, B. amyloliquefaciens DN6502 and B. licheniformis SDZD02) demonstrated the efficiency of natural bioproducts not only on curing diarrhea, but also on beneficial bacteria to re-establish in the piglet gut. We therefore propose a new natural “medicine” to be explored by the world farm animal agriculture industry, particularly for sustainable improvement of swine livestock production and health.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2020-11-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Curing piglets from diarrhea and preparation of a healthy microbiome with Bacillus treatment for industrial animal breeding.

Yue, Shousong / Li, Zhentian / Hu, Fuli / Picimbon, Jean-François

Scientific reports

2020 Volume 10, Issue 1, Page(s) 19476

Abstract: High-throughput farming of animals for an essential purpose such as large scale health and production of hogs is a challenge for the food industry in the modern world. The problem is that the breeding of livestock for fast growth or high yields of meat ... ...

Abstract	High-throughput farming of animals for an essential purpose such as large scale health and production of hogs is a challenge for the food industry in the modern world. The problem is that the breeding of livestock for fast growth or high yields of meat is often associated with illness and microbial infection that develop under the breeding conditions. Piglet diarrhea is most common pig disease, leading to heavy mortality and thereby economic loss. We proved that chemical drugs can relieve the symptoms of diarrhea in ill piglets, but they do not treat the underlying cause, i.e. significantly altered bacterial gut flora. Using Illumina sequencing of fecal DNA, we showed that the bacterial gut flora of piglets treated with antibiotics remain close to the ill conditions. However, using Illumina sequencing of fecal DNA from piglets treated with a specific Bacillus (Bacillus subtilis Y-15, B. amyloliquefaciens DN6502 and B. licheniformis SDZD02) demonstrated the efficiency of natural bioproducts not only on curing diarrhea, but also on beneficial bacteria to re-establish in the piglet gut. We therefore propose a new natural "medicine" to be explored by the world farm animal agriculture industry, particularly for sustainable improvement of swine livestock production and health.
MeSH term(s)	Animal Feed/analysis ; Animals ; Anti-Bacterial Agents/administration & dosage ; Bacillus/metabolism ; Bacillus/physiology ; Bacillus subtilis/metabolism ; Bacillus subtilis/physiology ; Bacteria/classification ; Bacteria/drug effects ; Bacteria/genetics ; Breeding/methods ; Diarrhea/microbiology ; Diarrhea/therapy ; Feces/microbiology ; High-Throughput Nucleotide Sequencing/methods ; Microbiota/genetics ; Probiotics/administration & dosage ; Probiotics/metabolism ; RNA, Ribosomal, 16S/genetics ; Swine ; Swine Diseases/microbiology ; Swine Diseases/therapy
Chemical Substances	Anti-Bacterial Agents ; RNA, Ribosomal, 16S
Keywords	covid19
Language	English
Publishing date	2020-11-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-75207-1
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Article ; Online: Alterations of Glucose Uptake and Protein Expression Related to the Insulin Signaling Pathway in the Brain of Phenobarbital-Addictive Rats by

Wang, Maolin / Pu, Xiaofeng / Feng, Bimin / Fan, Qingze / Dai, Yan / Chen, Yue / Li, Ying / Liu, Liang / Cao, Shousong / Wang, Guojun

Journal of proteome research

2020 Volume 20, Issue 1, Page(s) 950–959

Abstract: Drug addiction is a chronic relapsing brain disease. Alterations of glucose uptake and metabolism are found in the brain of drug addicts. Insulin mediates brain glucose metabolism and its abnormality could induce brain injury and cognitive impairment. ... ...

Abstract	Drug addiction is a chronic relapsing brain disease. Alterations of glucose uptake and metabolism are found in the brain of drug addicts. Insulin mediates brain glucose metabolism and its abnormality could induce brain injury and cognitive impairment. Here, we established a rat model of phenobarbital addiction by 90 days of dose escalation and evaluated addiction-related symptoms. We also performed
MeSH term(s)	Animals ; Brain/diagnostic imaging ; Chromatography, Liquid ; Fluorodeoxyglucose F18 ; Glucose ; Insulin ; Phenobarbital/toxicity ; Positron Emission Tomography Computed Tomography ; Proteomics ; Rats ; Signal Transduction ; Tandem Mass Spectrometry
Chemical Substances	Insulin ; Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; Glucose (IY9XDZ35W2) ; Phenobarbital (YQE403BP4D)
Language	English
Publishing date	2020-10-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.0c00703
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Article ; Online: A comprehensive alanine-scanning mutagenesis study reveals roles for salt bridges in the structure and activity of Pseudomonas aeruginosa elastase.

Fei Bian / Shousong Yue / Zhenying Peng / Xiaowei Zhang / Gao Chen / Jinhui Yu / Ning Xuan / Yuping Bi

PLoS ONE, Vol 10, Iss 3, p e

2015 Volume 0121108

Abstract: The relationship between salt bridges and stability/enzymatic activity is unclear. We studied this relationship by systematic alanine-scanning mutation analysis using the typical M4 family metalloprotease Pseudomonas aeruginosa elastase (PAE, also known ... ...

Abstract	The relationship between salt bridges and stability/enzymatic activity is unclear. We studied this relationship by systematic alanine-scanning mutation analysis using the typical M4 family metalloprotease Pseudomonas aeruginosa elastase (PAE, also known as pseudolysin) as a model. Structural analysis revealed seven salt bridges in the PAE structure. We constructed ten mutants for six salt bridges. Among these mutants, six (Asp189Ala, Arg179Ala, Asp201Ala, Arg205Ala, Arg245Ala and Glu249Ala) were active and four (Asp168Ala, Arg198Ala, Arg253Ala, and Arg279Ala) were inactive. Five mutants were purified, and their catalytic efficiencies (kcat/Km), half-lives (t1/2) and thermal unfolding curves were compared with those of PAE. Mutants Asp189Ala and Arg179Ala both showed decreased thermal stabilities and increased activities, suggesting that the salt bridge Asp189-Arg179 stabilizes the protein at the expense of catalytic efficiency. In contrast, mutants Asp201Ala and Arg205Ala both showed slightly increased thermal stability and slightly decreased activity, suggesting that the salt bridge Asp201-Arg205 destabilizes the protein. Mutant Glu249Ala is related to a C-terminal salt bridge network and showed both decreased thermal stability and decreased activity. Furthermore, Glu249Ala showed a thermal unfolding curve with three discernable states [the native state (N), the partially unfolded state (I) and the unfolded state (U)]. In comparison, there were only two discernable states (N and U) in the thermal unfolding curve of PAE. These results suggest that Glu249 is important for catalytic efficiency, stability and unfolding cooperativity. This study represents a systematic mutational analyses of salt bridges in the model metalloprotease PAE and provides important insights into the structure-function relationship of enzymes.
Keywords	Medicine ; R ; Science ; Q
Subject code	540
Language	English
Publishing date	2015-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A comprehensive alanine-scanning mutagenesis study reveals roles for salt bridges in the structure and activity of Pseudomonas aeruginosa elastase.

Bian, Fei / Yue, Shousong / Peng, Zhenying / Zhang, Xiaowei / Chen, Gao / Yu, Jinhui / Xuan, Ning / Bi, Yuping

PloS one

2015 Volume 10, Issue 3, Page(s) e0121108

Abstract	The relationship between salt bridges and stability/enzymatic activity is unclear. We studied this relationship by systematic alanine-scanning mutation analysis using the typical M4 family metalloprotease Pseudomonas aeruginosa elastase (PAE, also known as pseudolysin) as a model. Structural analysis revealed seven salt bridges in the PAE structure. We constructed ten mutants for six salt bridges. Among these mutants, six (Asp189Ala, Arg179Ala, Asp201Ala, Arg205Ala, Arg245Ala and Glu249Ala) were active and four (Asp168Ala, Arg198Ala, Arg253Ala, and Arg279Ala) were inactive. Five mutants were purified, and their catalytic efficiencies (kcat/Km), half-lives (t1/2) and thermal unfolding curves were compared with those of PAE. Mutants Asp189Ala and Arg179Ala both showed decreased thermal stabilities and increased activities, suggesting that the salt bridge Asp189-Arg179 stabilizes the protein at the expense of catalytic efficiency. In contrast, mutants Asp201Ala and Arg205Ala both showed slightly increased thermal stability and slightly decreased activity, suggesting that the salt bridge Asp201-Arg205 destabilizes the protein. Mutant Glu249Ala is related to a C-terminal salt bridge network and showed both decreased thermal stability and decreased activity. Furthermore, Glu249Ala showed a thermal unfolding curve with three discernable states [the native state (N), the partially unfolded state (I) and the unfolded state (U)]. In comparison, there were only two discernable states (N and U) in the thermal unfolding curve of PAE. These results suggest that Glu249 is important for catalytic efficiency, stability and unfolding cooperativity. This study represents a systematic mutational analyses of salt bridges in the model metalloprotease PAE and provides important insights into the structure-function relationship of enzymes.
MeSH term(s)	Alanine/chemistry ; Alanine/genetics ; Catalysis ; Enzyme Stability ; Mutagenesis, Site-Directed ; Pancreatic Elastase/chemistry ; Pancreatic Elastase/genetics ; Protein Conformation ; Pseudomonas aeruginosa/enzymology ; Pseudomonas aeruginosa/pathogenicity ; Salts/chemistry ; Structure-Activity Relationship
Chemical Substances	Salts ; Pancreatic Elastase (EC 3.4.21.36) ; Alanine (OF5P57N2ZX)
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0121108
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Article ; Online: Electroacupuncture improves cardiac function after myocardial infarction by regulating the mobilization and migration of endogenous stem cells.

Xuan, Shou-Song / Zhao, Yue / Zheng, Yan / Zhu, Jing / Li, Han / Lu, Ping-Ping / Shao, Shui-Jin / Guo, Hai-Dong / Mou, Fang-Fang

Acupuncture in medicine : journal of the British Medical Acupuncture Society

2023 Volume 41, Issue 6, Page(s) 354–363

Abstract: Objective: The aim of this study was to explore the role and mechanisms of electroacupuncture (EA) in the regulation of chemokines in endogenous stem cell mobilization and myocardial regeneration after myocardial infarction (MI).: Methods: An MI ... ...

Abstract	Objective: The aim of this study was to explore the role and mechanisms of electroacupuncture (EA) in the regulation of chemokines in endogenous stem cell mobilization and myocardial regeneration after myocardial infarction (MI). Methods: An MI model was constructed in adult male Sprague-Dawley rats by ligating the left anterior descending coronary artery. After 4 weeks of treatment, echocardiography was used to detect changes in cardiac function, and Masson's trichrome staining was used to detect collagen deposition. In addition, immunofluorescence staining was applied to examine von Willebrand factor (vWF)-positive vessels, the expression of cardiac troponin T (cTnT) and proliferation marker Ki67, and the number of c-kit-positive, C-X-C chemokine receptor type 4 (CXCR4)-positive, and Sca-1-positive endogenous stem cells in the infarcted area. In addition, the expression of stromal cell-derived factor (SDF)-1 and stem cell factor (SCF) was detected. Results: EA increased the ejection fraction after MI, reduced collagen deposition and cellular apoptosis, and increased the number of blood vessels compared with an untreated model group. EA significantly promoted cellular proliferation, except for myocardial cells, and significantly increased the number of c-kit-, CXCR4- and Sca-1-positive stem cells. Moreover, the expression of SDF-1 and SCF in myocardial tissue in the EA group was significantly higher than that in the (untreated) MI group. Conclusions: EA appears to promote angiogenesis and reduce collagen deposition, thus improving the cardiac function of rats with MI. The underlying mechanism of action may involve endogenous stem cell mobilization mediated by SDF-1/CXCR4 and SCF/c-kit.
MeSH term(s)	Rats ; Male ; Animals ; Rats, Sprague-Dawley ; Electroacupuncture ; Myocardial Infarction/therapy ; Stem Cells/metabolism ; Collagen
Chemical Substances	Collagen (9007-34-5)
Language	English
Publishing date	2023-06-19
Publishing country	England
Document type	Journal Article
ZDB-ID	1360533-1
ISSN	1759-9873 ; 0964-5284
ISSN (online)	1759-9873
ISSN	0964-5284
DOI	10.1177/09645284231169485
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Zs.A 4628: Show issues

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Article: [Expression and bioactivity assay of mature chicken interleukin-18 protein mutant using Pichia pastoris expression system].

Wang, Xihui / Yue, Shousong / Hu, Jingdong / Huang, Yijun / Chen, Jinlong / Wang, Tingting / Fan, Zhongling / Zhao, Hongkun

Wei sheng wu xue bao = Acta microbiologica Sinica

2010 Volume 50, Issue 9, Page(s) 1258–1263

Abstract: Objective: We expressed mature chicken interleukin-18 (mChIL-18) in Pichia pastoris.: Methods: The mChIL-18 gen was reconstructed by using site-specific mutagenesis based on the Pichia pastoris-preferred codons. The recombinant plasmid pPIC9K/mChIL- ... ...

Abstract	Objective: We expressed mature chicken interleukin-18 (mChIL-18) in Pichia pastoris. Methods: The mChIL-18 gen was reconstructed by using site-specific mutagenesis based on the Pichia pastoris-preferred codons. The recombinant plasmid pPIC9K/mChIL-18 was constructed and transformed to Pichia pastoris GS115 by electroperation. Multi-copy recombinant strains were screened by Geneticin (G418). The expression of mChIL-18 protein was induced by methanol. SDS-PAGE and Western-blot were used to analyze the expressed products. The bioactivity of mChIL-18 was measured by methyl thiazolyl tetrazolium assays and chicken embryo fibroblasts-vesicular stomatitis virus (CEF-VSV) system. Results: The protein of mChIL-18 could be secreted by GS115. The optimum expression conditions, a rate of 480 mg/L,were obtained as follows:temperature 28 degrees C, pH 6.5, methanol concentration 2% and expression time 120 h. The obtained mChIL-18 protein could stimulate T lymphocytes proliferation. IFN-gamma induced by mChIL-18 could directly inhibit the growth of VSV in CEF,and its antiviral activity was about 1.7 x 10(4) U/mL which was produced by 400 microg/L of mChIL-18. Conclusion: The high expression of bioactive recombinant mature chicken interleukin-18 (mChiL-18) in Pichia pastoris had been achieved.
MeSH term(s)	Animals ; Blotting, Western ; Chickens ; Electrophoresis, Polyacrylamide Gel ; Interleukin-18/genetics ; Interleukin-18/metabolism ; Mutant Proteins/genetics ; Mutant Proteins/metabolism ; Pichia/genetics ; Pichia/metabolism
Chemical Substances	Interleukin-18 ; Mutant Proteins
Language	Chinese
Publishing date	2010-09
Publishing country	China
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	412683-x
ISSN	0001-6209 ; 0098-9150
ISSN	0001-6209 ; 0098-9150
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