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  1. Article ; Online: Correction: Martínez-Murcia et al. SARS-CoV-2 Variants Identification; A Fast and Affordable Strategy Based on Partial S-Gene Targeted PCR Sequencing.

    Martínez-Murcia, Antonio / Garcia-Sirera, Adrian / Navarro, Aaron / Pérez, Laura

    Viruses

    2023  Volume 15, Issue 3

    Abstract: In the original publication [ ... ]. ...

    Abstract In the original publication [...].
    Language English
    Publishing date 2023-03-17
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15030771
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: qPCR Detection of Candida auris Using the GPS™ CanAur MONODOSE dtec-qPCR Test.

    Martínez-Murcia, Antonio / Bru, Gema / Navarro, Aaron

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2517, Page(s) 43–51

    Abstract: Candida auris is a multidrug-resistant pathogenic ascomycete yeast of increasing health concern. C. auris colonizes patient's skin and can persist for weeks on surfaces, so it can be transmitted within and between hospitals. The most common diagnostic ... ...

    Abstract Candida auris is a multidrug-resistant pathogenic ascomycete yeast of increasing health concern. C. auris colonizes patient's skin and can persist for weeks on surfaces, so it can be transmitted within and between hospitals. The most common diagnostic platforms in microbiology use reference databases that have not yet incorporated C. auris, misidentifying it. This chapter describes how to detect C. auris by qPCR with the GPS™ CanAur MONODOSE dtec-qPCR Test (Alicante, Spain) in less than 45 min, using ready-to-use tubes with all the components dehydrated. This commercial kit was subjected to validation following the guidelines of the UNE-EN ISO/IEC 17025:2005 and French Standard NF T90-471:2010.
    MeSH term(s) Antifungal Agents ; Candida/genetics ; Candida auris ; Humans ; Real-Time Polymerase Chain Reaction
    Chemical Substances Antifungal Agents
    Language English
    Publishing date 2022-06-08
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2417-3_4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Internal Validation of the ASFV MONODOSE dtec-qPCR Kit for African Swine Fever Virus Detection under the UNE-EN ISO/IEC 17025:2005 Criteria.

    Bru, Gema / Martínez-Candela, Marta / Romero, Paloma / Navarro, Aaron / Martínez-Murcia, Antonio

    Veterinary sciences

    2023  Volume 10, Issue 9

    Abstract: African swine fever virus is considered an emerging virus that causes African swine fever, a disease characterised by high mortality and elevated transmission rates and that, as it is for most other viral diseases, cannot be treated with specific drugs. ... ...

    Abstract African swine fever virus is considered an emerging virus that causes African swine fever, a disease characterised by high mortality and elevated transmission rates and that, as it is for most other viral diseases, cannot be treated with specific drugs. Effective and reliable detection of the virus is relevant to prevent uncontrolled contagion among boar populations and to reduce economic losses. Moreover, animal health laboratories are demanding standardisation, optimisation and quality assurance of the available diagnostic assays. In the present study, the ASFV MONODOSE dtec-qPCR kit was validated following the UNE-EN ISO/IEC 17025:2005 guidelines. Analytical validation terms include in silico and in vitro specificity, sensitivity, efficiency and reliability (repeatability/reproducibility). Diagnostic validation of the method was assessed through the analysis of a total of 181 porcine samples originating from six different matrix types doped with African swine fever virus DNA received from the European reference laboratory for African Swine Fever (INIA-CISA, Madrid, Spain): whole blood, blood serum, kidney, heart, liver and tonsil. Results agreed with those obtained from a reference detection method also based on real-time PCR, endorsed by WOAH, but the ASFV MONODOSE dtec-qPCR kit incorporates some technical innovations and improvements which may benefit end-users. This kit, available worldwide with full analytical and diagnostic validation, can recognise all known ASFV genotypes and brings additional benefits to the current qPCR technology.
    Language English
    Publishing date 2023-09-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci10090564
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Internal Validation of a Real-Time qPCR Kit following the UNE/EN ISO/IEC 17025:2005 for Detection of the Re-Emerging

    Martínez-Murcia, Antonio / Navarro, Aaron / Garcia-Sirera, Adrian / Pérez, Laura / Bru, Gema

    Diagnostics (Basel, Switzerland)

    2023  Volume 13, Issue 9

    Abstract: Human mpox is caused by ... ...

    Abstract Human mpox is caused by the
    Language English
    Publishing date 2023-04-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics13091560
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  5. Article ; Online: SARS-CoV-2 Variants Identification; A Fast and Affordable Strategy Based on Partial S-Gene Targeted PCR Sequencing.

    Martínez-Murcia, Antonio / Garcia-Sirera, Adrian / Navarro, Aaron / Pérez, Laura

    Viruses

    2022  Volume 14, Issue 11

    Abstract: A considerable number of new SARS-CoV-2 lineages have emerged since the first COVID-19 cases were reported in Wuhan. As a few variants showed higher COVID-19 disease transmissibility and the ability to escape from immune responses, surveillance became ... ...

    Abstract A considerable number of new SARS-CoV-2 lineages have emerged since the first COVID-19 cases were reported in Wuhan. As a few variants showed higher COVID-19 disease transmissibility and the ability to escape from immune responses, surveillance became relevant at that time. Single-nucleotide mutation PCR-based protocols were not always specific, and consequently, determination of a high number of informative sites was needed for accurate lineage identification. A detailed in silico analysis of SARS-CoV-2 sequences retrieved from GISAID database revealed the S gene 921 bp-fragment, positions 22784-23705 of SARS-CoV-2 reference genome, as the most informative fragment (30 variable sites) to determine relevant SARS-CoV-2 variants. Consequently, a method consisting of the PCR-amplification of this fragment, followed by Sanger's sequencing and a "single-click" informatic program based on a reference database, was developed and validated. PCR-fragments obtained from clinical SARS-CoV-2 samples were compared with homologous variant-sequences and the resulting phylogenetic tree allowed the identification of Alpha, Delta, Omicron, Beta, Gamma, and other variants. The data analysis procedure was automatized and simplified to the point that it did not require specific technical skills. The method is faster and cheaper than current whole-genome sequencing methods; it is available worldwide, and it may help to enhance efficient surveillance in the fight against the COVID-19 pandemic.
    MeSH term(s) Humans ; SARS-CoV-2/genetics ; Phylogeny ; Genome, Viral ; COVID-19/diagnosis ; COVID-19/epidemiology ; Pandemics ; Polymerase Chain Reaction
    Language English
    Publishing date 2022-11-21
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14112588
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Current RT-qPCR to detect SARS-CoV-2 may give positive results for related coronaviruses.

    Martínez-Murcia, Antonio / García-Sirera, Adrián / Navarro, Aaron / Pérez, Laura

    Archives of microbiology

    2022  Volume 204, Issue 7, Page(s) 415

    Abstract: Some weeks after the first CoVID-19 outbreak, the World Health Organization published some real-time PCR (qPCR) protocols developed by different health reference centers. These qPCR designs are being used worldwide to detect SARS-CoV-2 in the population, ...

    Abstract Some weeks after the first CoVID-19 outbreak, the World Health Organization published some real-time PCR (qPCR) protocols developed by different health reference centers. These qPCR designs are being used worldwide to detect SARS-CoV-2 in the population, to monitor the prevalence of the virus during the pandemic. Moreover, some of these protocols to detect SARS-CoV-2 have widely been applied to environmental samples for epidemiological surveillance purposes. In the present work, the specificity of these currently used RT-qPCR designs was validated in vitro using SARS-CoV-2 and highly related coronaviral genomic sequences and compared to performance of the commercially available GPS™ CoVID-19 dtec-RT-qPCR Test. Assays performed with SARS-CoV-2-related genomes showed positive amplification when using some of these qPCR methods, indicating they may give SARS-CoV-2 false positives. This finding may be particularly relevant for SARS-CoV-2 monitoring of environmental samples, where an unknown pool of phylogenetically close-related viruses may exist.
    MeSH term(s) COVID-19/diagnosis ; Humans ; Pandemics ; Real-Time Polymerase Chain Reaction/methods ; SARS-CoV-2/genetics ; Sensitivity and Specificity
    Language English
    Publishing date 2022-06-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-022-03029-y
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    In stock of ZB MED Cologne/Königswinter

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    Jg. 1995 - 2021: Lesesall (1.OG)
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  7. Article: Current RT-qPCR to detect SARS-CoV-2 may give positive results for related coronaviruses

    Martínez-Murcia, Antonio / García-Sirera, Adrián / Navarro, Aaron / Pérez, Laura

    Archives of microbiology. 2022 July, v. 204, no. 7

    2022  

    Abstract: Some weeks after the first CoVID-19 outbreak, the World Health Organization published some real-time PCR (qPCR) protocols developed by different health reference centers. These qPCR designs are being used worldwide to detect SARS-CoV-2 in the population, ...

    Abstract Some weeks after the first CoVID-19 outbreak, the World Health Organization published some real-time PCR (qPCR) protocols developed by different health reference centers. These qPCR designs are being used worldwide to detect SARS-CoV-2 in the population, to monitor the prevalence of the virus during the pandemic. Moreover, some of these protocols to detect SARS-CoV-2 have widely been applied to environmental samples for epidemiological surveillance purposes. In the present work, the specificity of these currently used RT-qPCR designs was validated in vitro using SARS-CoV-2 and highly related coronaviral genomic sequences and compared to performance of the commercially available GPS™ CoVID-19 dtec-RT-qPCR Test. Assays performed with SARS-CoV-2-related genomes showed positive amplification when using some of these qPCR methods, indicating they may give SARS-CoV-2 false positives. This finding may be particularly relevant for SARS-CoV-2 monitoring of environmental samples, where an unknown pool of phylogenetically close-related viruses may exist.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; World Health Organization ; genome ; genomics ; monitoring ; pandemic ; phylogeny ; quantitative polymerase chain reaction ; viruses
    Language English
    Dates of publication 2022-07
    Size p. 415.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-022-03029-y
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    In stock of ZB MED Bonn / Germany

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  8. Article: Assessment of ISO Method 15216 to Quantify Hepatitis E Virus in Bottled Water.

    Cuevas-Ferrando, Enric / Martínez-Murcia, Antonio / Pérez-Cataluña, Alba / Sánchez, Gloria / Randazzo, Walter

    Microorganisms

    2020  Volume 8, Issue 5

    Abstract: Hepatitis E virus (HEV) is one of the causative agents of water-borne human viral hepatitis and considered in Europe an emerging zoonotic pathogen. Analysis of bottled water through a standard method validated for HEV can contribute towards the risk ... ...

    Abstract Hepatitis E virus (HEV) is one of the causative agents of water-borne human viral hepatitis and considered in Europe an emerging zoonotic pathogen. Analysis of bottled water through a standard method validated for HEV can contribute towards the risk management of this hazard. Putting some recent reports by the European Food Safety Authority in place, this study aimed to assess the performance of the concentration and extraction procedures described in ISO 15216-1:2017 for norovirus and hepatitis A virus on HEV detection. Following the ISO recommendation, the bottled water samples were spiked using serially diluted HEV fecal suspensions together with mengovirus as process control and concentrated by filtration via positively charged nylon membranes. In order to extract viral RNA from the resulting concentrates, two different methods were compared in this study: The one recommended in the ISO norm, NucliSens® MiniMag® system (NS), and an alternative commercially available kit NucleoSpin®RNA virus kit (MN). Finally, three reverse transcription quantitative PCR (RT-qPCR) assays were used to quantify HEV titers. The evaluated procedures resulted in average HEV recoveries of 14.08 ± 4.90% and 3.58 ± 0.30% for the MN and NS methods, respectively. The limit of detection (LoD
    Language English
    Publishing date 2020-05-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms8050730
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: First Record of the Rare Species

    Fernández-Bravo, Ana / Vega-Sánchez, Vicente / Pérez-Cataluña, Alba / Latif-Eugenín, Fadua / Beaz-Hidalgo, Roxana / Martínez-Murcia, Antonio / Soriano-Vargas, Edgardo / Cabrero-Martínez, Omar Alejandro / Castro-Escarpulli, Graciela / Figueras, Maria José

    Pathogens (Basel, Switzerland)

    2022  Volume 11, Issue 11

    Abstract: ... The ... ...

    Abstract The species
    Language English
    Publishing date 2022-11-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens11111299
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Internal validation of GPS

    Martínez-Murcia, Antonio / Navarro, Aaron / Bru, Gema / Chowdhary, Anuradha / Hagen, Ferry / Meis, Jacques F

    Mycoses

    2018  Volume 61, Issue 11, Page(s) 877–884

    Abstract: Candida auris is an emerging multidrug resistant pathogenic fungus that causes candidaemia with high mortality rates and exhibits the ability to persist within the hospital environment. Candida auris is phylogenetically closely related to Candida ... ...

    Abstract Candida auris is an emerging multidrug resistant pathogenic fungus that causes candidaemia with high mortality rates and exhibits the ability to persist within the hospital environment. Candida auris is phylogenetically closely related to Candida haemulonii, C. lusitaniae, C. pseudohaemulonii and C. duobushaemulonii and is frequently misidentified by commercial identification methods. In the present study, the GPS
    MeSH term(s) Africa ; Candida/classification ; Candida/genetics ; Candida/isolation & purification ; Candidiasis/diagnosis ; Candidiasis/microbiology ; Europe ; Humans ; Mycological Typing Techniques ; Real-Time Polymerase Chain Reaction/methods ; Reproducibility of Results
    Language English
    Publishing date 2018-08-20
    Publishing country Germany
    Document type Journal Article ; Validation Studies
    ZDB-ID 392487-7
    ISSN 1439-0507 ; 0933-7407
    ISSN (online) 1439-0507
    ISSN 0933-7407
    DOI 10.1111/myc.12834
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.176: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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