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  1. Article ; Online: Current and future molecular diagnostics in colorectal cancer and colorectal adenoma.

    Tsang, Andy Hin-Fung / Cheng, Ka-Ho / Wong, Apple Siu-Ping / Ng, Simon Siu-Man / Ma, Brigette Buig-Yue / Chan, Charles Ming-Lok / Tsui, Nancy Bo-Yin / Chan, Lawrence Wing-Chi / Yung, Benjamin Yat-Ming / Wong, Sze-Chuen Cesar

    World journal of gastroenterology

    2014  Volume 20, Issue 14, Page(s) 3847–3857

    Abstract: Colorectal cancer (CRC) is one of the most prevalent cancers in developed countries. On the other hand, CRC is also one of the most curable cancers if it is detected in early stages through regular colonoscopy or sigmoidoscopy. Since CRC develops slowly ... ...

    Abstract Colorectal cancer (CRC) is one of the most prevalent cancers in developed countries. On the other hand, CRC is also one of the most curable cancers if it is detected in early stages through regular colonoscopy or sigmoidoscopy. Since CRC develops slowly from precancerous lesions, early detection can reduce both the incidence and mortality of the disease. Fecal occult blood test is a widely used non-invasive screening tool for CRC. Although fecal occult blood test is simple and cost-effective in screening CRC, there is room for improvement in terms of the accuracy of the test. Genetic dysregulations have been found to play an important role in CRC development. With better understanding of the molecular basis of CRC, there is a growing expectation on the development of diagnostic tests based on more sensitive and specific molecular markers and those tests may provide a breakthrough to the limitations of current screening tests for CRC. In this review, the molecular basis of CRC development, the characteristics and applications of different non-invasive molecular biomarkers, as well as the technologies available for the detection were discussed. This review intended to provide a summary on the current and future molecular diagnostics in CRC and its pre-malignant state, colorectal adenoma.
    MeSH term(s) Adenoma/diagnosis ; Adenoma/genetics ; Biomarkers, Tumor/metabolism ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/epidemiology ; Colorectal Neoplasms/genetics ; Cost-Benefit Analysis ; DNA, Neoplasm/metabolism ; Epigenesis, Genetic ; Feces ; Gene Silencing ; Humans ; MicroRNAs/metabolism ; Microsatellite Instability ; Occult Blood ; Pathology, Molecular/methods ; Pathology, Molecular/trends ; Reproducibility of Results
    Chemical Substances Biomarkers, Tumor ; DNA, Neoplasm ; MicroRNAs
    Language English
    Publishing date 2014-03-12
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2185929-2
    ISSN 2219-2840 ; 1007-9327
    ISSN (online) 2219-2840
    ISSN 1007-9327
    DOI 10.3748/wjg.v20.i14.3847
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: Application of NanoString technologies in companion diagnostic development.

    Eastel, Jennifer Mary / Lam, Ka Wai / Lee, Nga Lam / Lok, Wing Yan / Tsang, Andy Hin Fung / Pei, Xiao Meng / Chan, Amanda Kit Ching / Cho, William Chi Shing / Wong, Sze Chuen Cesar

    Expert review of molecular diagnostics

    2019  Volume 19, Issue 7, Page(s) 591–598

    Abstract: ... ...

    Abstract Introduction
    MeSH term(s) Biomarkers ; Gene Expression Profiling/methods ; Humans ; Molecular Diagnostic Techniques ; Nanotechnology ; Precision Medicine/methods ; Precision Medicine/standards ; Prognosis ; RNA, Messenger/genetics ; Theranostic Nanomedicine/methods ; Theranostic Nanomedicine/standards
    Chemical Substances Biomarkers ; RNA, Messenger
    Language English
    Publishing date 2019-06-05
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2112530-2
    ISSN 1744-8352 ; 1473-7159
    ISSN (online) 1744-8352
    ISSN 1473-7159
    DOI 10.1080/14737159.2019.1623672
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing.

    Xue, Vivian W / Cheung, Moon T / Chan, Pak T / Luk, Lewis L Y / Lee, Vivian H / Au, Thomas C / Yu, Allen C / Cho, William C S / Tsang, Hin Fung Andy / Chan, Amanda K / Wong, S C Cesar

    Scientific reports

    2019  Volume 9, Issue 1, Page(s) 12943

    Abstract: We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential ... ...

    Abstract We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed that GSK3A and RHOA were differential expressed genes identified by a cut-off of fold change >2 and adjusted P value < 0.05. More detailed analysis showed that the expression of both GSK3A (0.01-fold with adjusted P < 1 × 10
    MeSH term(s) Adenoma/blood ; Adenoma/genetics ; Adenoma/pathology ; Biomarkers, Tumor/blood ; Biomarkers, Tumor/genetics ; Case-Control Studies ; Colorectal Neoplasms/blood ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/pathology ; Gene Expression Profiling/methods ; Gene Expression Regulation, Neoplastic ; Humans ; Prognosis ; RNA, Messenger/blood ; RNA, Messenger/genetics
    Chemical Substances Biomarkers, Tumor ; RNA, Messenger
    Language English
    Publishing date 2019-09-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-019-49445-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Hotspot

    Wong, Hung Lai / Ng, Lawrence Po Wah / Koh, Su Pin / Chan, Lawrence Wing Chi / Wong, Evelyn Yin Kwan / Xue, Vivian Weiwen / Tsang, Hin Fung Andy / Chan, Amanda Kit Ching / Chiu, Ka Yue / Cheuk, Wah / Wong, Sze Chuen Cesar

    Oncotarget

    2018  Volume 9, Issue 29, Page(s) 20426–20438

    Abstract: Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer deaths worldwide. Recent studies have shown that cancer stem cells (CSCs) are an important cause of tumor recurrence and metastasis. We hypothesized that CSCs ... ...

    Abstract Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer deaths worldwide. Recent studies have shown that cancer stem cells (CSCs) are an important cause of tumor recurrence and metastasis. We hypothesized that CSCs marker CD166-positive CRC and colorectal adenoma (CAD) cells consist of more hotspot mutations than CD166-negative CRC and colorectal adenoma cells. To verify this, formalin fixed paraffin embedded tissue specimens from 42 patients each with CRC and CAD were recruited and CD166 immunohistochemical (IHC) staining followed by macrodissection was performed. DNA extracted was used for quantitative polymerase chain reaction detection on a somatic mutation array. Results showed that the immunoreactivity of CD166 protein had significant difference among CRC, CAD, and normal colorectal epithelial tissues (NCET) (
    Language English
    Publishing date 2018--17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2560162-3
    ISSN 1949-2553 ; 1949-2553
    ISSN (online) 1949-2553
    ISSN 1949-2553
    DOI 10.18632/oncotarget.24921
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: The Effect of Centrifugal Force in Quantification of Colorectal Cancer-Related mRNA in Plasma Using Targeted Sequencing.

    Xue, Vivian Weiwen / Ng, Simon Siu Man / Leung, Wing Wa / Ma, Brigette Buig Yue / Cho, William Chi Shing / Au, Thomas Chi Chuen / Yu, Allen Chi Shing / Tsang, Hin Fung Andy / Wong, Sze Chuen Cesar

    Frontiers in genetics

    2018  Volume 9, Page(s) 165

    Abstract: In our previous study, we detected the effects of centrifugal forces on plasma RNA quantification by quantitative reverse transcription PCR. The aims of this study were to perform targeted mRNA sequencing and data analysis in healthy donors' plasma ... ...

    Abstract In our previous study, we detected the effects of centrifugal forces on plasma RNA quantification by quantitative reverse transcription PCR. The aims of this study were to perform targeted mRNA sequencing and data analysis in healthy donors' plasma prepared by two centrifugation protocols and to investigate the effects of centrifugal forces on plasma mRNA quality and quantity. Targeted mRNA sequencing was performed using a custom panel with 108 colorectal cancer-related genes in 18 healthy donors' plasma that prepared by (1) 3,500 g for 10 min at 4°C and (2) 1,600 g for 10 min at 4°C followed by 16,000 g for 10 min at 4°C. Results showed that plasma ribosomal RNA was detected in 16/18 (88.9%) 3,500 g and 6/18 (33.3%) 1,600 g followed by 16,000 g centrifuged plasma. For targeted sequencing, 75/108 (69.4%) and 86/108 (79.6%) genes were detected in 3,500 and 1,600 g followed by 16,000 g, respectively, while 16/108 (14.8%) genes were not detected in both centrifugations. Detailed analysis showed that 2 of 108 (1.85%) genes showed lower expressions in 3,500 g than in 1,600 g followed by 16,000 g. The median expressions of genes in 3,500 g were positively correlated with the expressions in 1,600 g followed by 16,000 g (
    Language English
    Publishing date 2018-05-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2018.00165
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Prognostic significance of Cytokeratin 20-positive lymph node vascular endothelial growth factor A mRNA and chromodomain helicase DNA binding protein 4 in pN0 colorectal cancer patients.

    Wong, Sze Chuen Cesar / Cheung, Moon Tong / Luk, Lewis Lai Yin / Lee, Vivian Ha Man / Chan, Pak Tat / Tsang, Hin Fung Andy / Wong, Evelyn Yin Kwan / Xue, Vivian Weiwen / Chan, Amanda Kit Ching / Chan, John Kwok Cheung

    Oncotarget

    2018  Volume 9, Issue 6, Page(s) 6737–6751

    Abstract: Background: Cytokeratin 20-positive cells in lymph nodes from pN0 colorectal cancer (CRC) patients were detected previously by us. The aims of this study were to investigate which tumor metastasis-related genes were involved and their potential clinical ...

    Abstract Background: Cytokeratin 20-positive cells in lymph nodes from pN0 colorectal cancer (CRC) patients were detected previously by us. The aims of this study were to investigate which tumor metastasis-related genes were involved and their potential clinical significance.
    Results: Fourteen of 84 (17%) genes were differentially expressed by at least 2-fold. Among them, 10 genes were up-regulated whereas 4 genes were down-regulated. Those differential expressed genes were validated in the second cohort of specimens. Follow-up analysis for 60 months showed that patients with lymph node
    Conclusions: VEGF-A
    Methods: Two cohorts of lymph node specimens from pN0 CRC patients of each with and without CK20-positive cells were recruited. In the first cohort, tumor metastasis genes were profiled using gene expression arrays. Differential expressed genes were validated in the second cohort. Moreover, their prognostic significance was examined by following-up the second cohort of patients with CK20-positive cells for 60 months and all histopathological findings were correlated to recurrence.
    Language English
    Publishing date 2018-01-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2560162-3
    ISSN 1949-2553 ; 1949-2553
    ISSN (online) 1949-2553
    ISSN 1949-2553
    DOI 10.18632/oncotarget.23424
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing

    Vivian W. Xue / Moon T. Cheung / Pak T. Chan / Lewis L. Y. Luk / Vivian H. Lee / Thomas C. Au / Allen C. Yu / William C. S. Cho / Hin Fung Andy Tsang / Amanda K. Chan / S. C. Cesar Wong

    Scientific Reports, Vol 9, Iss 1, Pp 1-

    2019  Volume 10

    Abstract: Abstract We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find ... ...

    Abstract Abstract We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed that GSK3A and RHOA were differential expressed genes identified by a cut-off of fold change >2 and adjusted P value < 0.05. More detailed analysis showed that the expression of both GSK3A (0.01-fold with adjusted P < 1 × 10−6) and RHOA (0.35-fold with adjusted P < 0.01) in adenoma patients was significantly lower than those in normal healthy subjects. Based on the enrichment analysis of biological process for potential markers, we found that the regulation of programmed cell death (GO: 0043067; GO: 0043069), regulation of cell death (GO: 0010941; GO: 0060548) and cell differentiation (GO: 0021861) were the main processes involved in adenoma formation. In summary, this study is a cutting-edge research on the detection of plasma mRNA in colorectal adenoma patients and normal healthy subjects.
    Keywords Medicine ; R ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2019-09-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Machine learning model to predict recurrent ulcer bleeding in patients with history of idiopathic gastroduodenal ulcer bleeding.

    Wong, Grace Lai-Hung / Ma, Andy Jinhua / Deng, Huiqi / Ching, Jessica Yuet-Ling / Wong, Vincent Wai-Sun / Tse, Yee-Kit / Yip, Terry Cheuk-Fung / Lau, Louis Ho-Shing / Liu, Henry Hin-Wai / Leung, Chi-Man / Tsang, Steven Woon-Choy / Chan, Chun-Wing / Lau, James Yun-Wong / Yuen, Pong-Chi / Chan, Francis Ka-Leung

    Alimentary pharmacology & therapeutics

    2019  Volume 49, Issue 7, Page(s) 912–918

    Abstract: Background: Patients with a history of Helicobacter pylori-negative idiopathic bleeding ulcers have an increased risk of recurring ulcer complications.: Aim: To build a machine learning model to identify patients at high risk for recurrent ulcer ... ...

    Abstract Background: Patients with a history of Helicobacter pylori-negative idiopathic bleeding ulcers have an increased risk of recurring ulcer complications.
    Aim: To build a machine learning model to identify patients at high risk for recurrent ulcer bleeding.
    Methods: Data from a retrospective cohort of 22 854 patients (training cohort) diagnosed with peptic ulcer disease in 2007-2016 were analysed to build a model (IPU-ML) to predict recurrent ulcer bleeding. We tested the IPU-ML in all patients with a diagnosis of gastrointestinal bleeding (n = 1265) in 2008-2015 from a different catchment population (independent validation cohort). Any co-morbid conditions which had occurred in >1% of study population were eligible as predictors.
    Results: Recurrent ulcer bleeding developed in 4772 patients (19.5%) in the training cohort, during a median follow-up period of 2.7 years. IPU-ML model built on six parameters (age, baseline haemoglobin, and presence of gastric ulcer, gastrointestinal diseases, malignancies, and infections) identified patients with bleeding recurrence within 1 year with an area under the receiver operating characteristic curve (AUROC) of 0.648. When we set the IPU-ML cutoff value at 0.20, 27.5% of patients were classified as high risk for rebleeding with a sensitivity of 41.4%, specificity of 74.6%, and a negative predictive value of 91.1%. In the validation cohort, the IPU-ML identified patients with a recurrence ulcer bleeding within 1 year with an AUROC of 0.775, and 84.3% of overall accuracy.
    Conclusion: We developed a machine-learning model to identify those patients with a history of idiopathic gastroduodenal ulcer bleeding who are not at high risk for recurrent ulcer bleeding.
    MeSH term(s) Adult ; Aged ; Cohort Studies ; Duodenal Ulcer/diagnosis ; Duodenal Ulcer/epidemiology ; Female ; Follow-Up Studies ; Gastrointestinal Hemorrhage/diagnosis ; Gastrointestinal Hemorrhage/epidemiology ; Helicobacter Infections/diagnosis ; Helicobacter Infections/epidemiology ; Helicobacter pylori ; Humans ; Machine Learning ; Male ; Middle Aged ; Prospective Studies ; Recurrence ; Retrospective Studies ; Stomach Ulcer/diagnosis ; Stomach Ulcer/epidemiology
    Language English
    Publishing date 2019-02-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639012-2
    ISSN 1365-2036 ; 0269-2813 ; 0953-0673
    ISSN (online) 1365-2036
    ISSN 0269-2813 ; 0953-0673
    DOI 10.1111/apt.15145
    Database MEDical Literature Analysis and Retrieval System OnLINE

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