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  1. Article: Synergistic impact of Gui Zhi Shao Yao Zhi Mu Decoction and leflunomide on gut microbiota in rheumatoid arthritis: insights from 16S rDNA sequencing.

    Sun, Ying / Chen, Xiaoheng / Zhang, Ye / Chen, Xiaojun / Zhang, Chen / Zhao, Jing / Sun, Songge / Zhang, Yanzhen / Qiu, Xinping

    American journal of translational research

    2024  Volume 16, Issue 2, Page(s) 654–668

    Abstract: ... including alterations in the gut microbiota. Gui Zhi Shao Yao Zhi Mu Decoction (GSZD), a traditional Chinese ...

    Abstract Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease with complex pathogenesis, including alterations in the gut microbiota. Gui Zhi Shao Yao Zhi Mu Decoction (GSZD), a traditional Chinese herbal formula, has shown efficacy in RA treatment, but its impact on intestinal microflora remains unclear. This study aimed to investigate the effects of GSZD combined with leflunomide on the gut microbiota of RA patients.
    Methods: The study enrolled 48 RA patients who were randomly assigned to either a control group receiving leflunomide or a treatment group receiving GSZD combined with leflunomide for 12 weeks. Gut microbiota composition was analyzed pre- and post-intervention using 16S rDNA sequencing. Changes in microbial diversity, abundance, and metabolic functions were assessed.
    Results: Post-treatment, both groups exhibited significant alterations in gut microbiota composition. GSZD combined with leflunomide led to an increased Bacteroidetes/Firmicutes ratio and a reduction in Actinobacteria compared to leflunomide alone. This was associated with beneficial shifts in microbial genera and metabolic pathways, suggesting improved gut health and systemic immune modulation.
    Conclusion: GSZD combined with leflunomide significantly modulates the gut microbiota in RA patients. This study provides insights into the mechanisms underlying the therapeutic effects of GSZD and highlights the potential of integrating traditional Chinese medicine with conventional treatments in managing RA.
    Language English
    Publishing date 2024-02-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2471058-1
    ISSN 1943-8141
    ISSN 1943-8141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Investigation of the mechanisms and experimental verification of Shao yao gan cao decoction against Sphincter of Oddi Dysfunction via systems pharmacology.

    Hu, Yong-Hong / Wang, Xue-Ying / Zhang, Xi-Wen / Chen, Jian / Li, Fu

    Mathematical biosciences and engineering : MBE

    2023  Volume 19, Issue 12, Page(s) 13374–13398

    Abstract: This study explored the chemical and pharmacological mechanisms of Shao Yao Gan Cao decoction (SYGC ...

    Abstract This study explored the chemical and pharmacological mechanisms of Shao Yao Gan Cao decoction (SYGC) in the treatment of Sphincter of Oddi Dysfunction (SOD) through ultra-high-performance liquid chromatography coupled with Quadrupole Exactive-Orbitrap high-resolution mass spectrometry (UHPLC-Q Exactive-Orbitrap HR-MS), network pharmacology, transcriptomics, molecular docking and in vivo experiments. First, we identified that SYGC improves SOD in guinea pigs by increased c-kit expression and decreased inflammation infiltration and ring muscle disorders. Then, a total of 649 SOD differential genes were found through RNA sequencing and mainly enriched in complement and coagulation cascades, the B cell receptor signaling pathway and the NF-kappa B signaling pathway. By combining UHPLC-Q-Orbitrap-HRMS with a network pharmacology study, 111 chemicals and a total of 52 common targets were obtained from SYGC in the treatment of SOD, which is also involved in muscle contraction, the B cell receptor signaling pathway and the complement system. Next, 20 intersecting genes were obtained among the PPI network, MCODE and ClusterOne analysis. Then, the molecular docking results indicated that four active compounds (glycycoumarin, licoflavonol, echinatin and homobutein) and three targets (AURKB, KIF11 and PLG) exerted good binding interactions, which are also related to the B cell receptor signaling pathway and the complement system. Finally, animal experiments were conducted to confirm the SYGC therapy effects on SOD and verify the 22 hub genes using RT-qPCR. This study demonstrates that SYGC confers therapeutic effects against an experimental model of SOD via regulating immune response and inflammation, which provides a basis for future research and clinical applications.
    MeSH term(s) Animals ; Guinea Pigs ; Glycyrrhiza uralensis ; Network Pharmacology ; Tandem Mass Spectrometry/methods ; Molecular Docking Simulation ; Sphincter of Oddi Dysfunction ; Inflammation/drug therapy ; Receptors, Antigen, B-Cell
    Chemical Substances Receptors, Antigen, B-Cell
    Language English
    Publishing date 2023-01-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2265126-3
    ISSN 1551-0018 ; 1551-0018
    ISSN (online) 1551-0018
    ISSN 1551-0018
    DOI 10.3934/mbe.2022626
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book: MicroRNA protocols

    Ying, Shao-Yao

    edited by Shao-Yao Ying

    (Methods in molecular biology ; 1733 ; Springer protocols)

    2018  

    Series title Methods in molecular biology ; 1733
    Springer protocols
    Collection
    Keywords Non-coding RNAs ; miRNA ; High-Throughput Expression ; Cell Death ; Drosophila ; cell culture ; stem cells ; gene silencing
    Subject code 610
    Language English
    Size xi, 319 Seiten, Illustrationen, 25.4 cm x 17.8 cm
    Edition Third edition
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT019605546
    ISBN 978-1-4939-7600-3 ; 1-4939-7600-1 ; 9781493976010 ; 149397601X
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1733- verfügbar in Königswinter Königswinter

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  4. Book: MicroRNA protocols

    Ying, Shao-Yao

    (Methods in molecular biology ; 936)

    2013  

    Author's details ed. by Shao-Yao Ying
    Series title Methods in molecular biology ; 936
    Collection
    Keywords MicroRNAs ; Niedermolekulare RNS ; Labortechnik
    Subject Laboratorium ; Laboratoriumstechnik ; Labormethode ; Kurzkettige RNS
    Language English
    Size XI, 375 S. : Ill., graph. Darst.
    Edition 2. ed.
    Publisher Humana Press
    Publishing place New York
    Publishing country United States
    Document type Book
    HBZ-ID HT017427396
    ISBN 978-1-62703-082-3 ; 978-1-62703-083-0 ; 1-62703-082-4 ; 1-62703-083-2
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2012 A 4238 order for loan Magazin

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  5. Book: MicroRNA protocols

    Ying, Shao-Yao

    (Methods in molecular biology ; 342)

    2006  

    Author's details ed. by Shao-Yao Ying
    Series title Methods in molecular biology ; 342
    Collection
    Keywords MicroRNAs ; Niedermolekulare RNS ; Labortechnik
    Subject Laboratorium ; Laboratoriumstechnik ; Labormethode ; Kurzkettige RNS
    Language English
    Size XIII, 365 S. : Ill., graph. Darst.
    Publisher Humana Press
    Publishing place Totowa, NJ
    Publishing country United States
    Document type Book
    HBZ-ID HT014692064
    ISBN 1-58829-581-8 ; 978-1-58829-581-1
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2006 A 2601 order for loan Magazin

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  6. Article ; Online: MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302.

    Lin, Shi-Lung / Chen, Jack S / Ying, Shao-Yao

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2115, Page(s) 199–219

    Abstract: Human induced pluripotent stem cells (iPSCs) by four factors have the risks of teratoma formation and potential tumorigenicity. To overcome this major hurdle, we examined the mechanism(s) by which the cell cycle genes of embryonic cells were regulated. ... ...

    Abstract Human induced pluripotent stem cells (iPSCs) by four factors have the risks of teratoma formation and potential tumorigenicity. To overcome this major hurdle, we examined the mechanism(s) by which the cell cycle genes of embryonic cells were regulated. Naturally occurring embryonic stem cells (ESCs) possess two unique stemness properties: pluripotent differentiation into all cell types and self-renewal with no risk of tumor formation. Despite overwhelming reports describing iPSC pluripotency, there have been no observations of tumor prevention mechanism that suppresses tumor formation similar to that in naturally occurring ESCs. The ESC-specific microRNA (miRNA), miR-302, regulates human iPSC tumorigenicity through co-suppression of both cyclin E-CDK2 and cyclin D-CDK4/6 cell cycle pathways during G1-S phase transition. MiR-302 also silenced BMI-1, a cancer stem cell marker gene, to promote the expression of two senescence-associated tumor suppressor genes, p16Ink4a and p14/p19Arf. Together, the combinatory effect of reducing G1-S cell cycle transition and increasing p16/p14(p19) expression resulted in a relatively attenuated cell cycle rate similar to that of 2-to-8-cell-stage embryonic cells in early mammalian zygotes (20-24 h/cycle), as compared to the fast proliferation rate of iPSCs induced by four defined factors Oct4-Sox2-Klf4-c-Myc (12-16 h/cycle). In addition to the prevention of stem cell tumorigenicity, the mechanism underlying miR-302-mediated iPSCs also includes the initiation of global genomic DNA methylation, activation of ESC-specific gene expression, and inhibition of developmental signaling. Overall, we have established an effective protocol to express the intronic miR-302 cluster, according to its own natural biogenesis mechanism to generate tumor-free iPSCs for use in biology and therapy.
    MeSH term(s) Carcinogenesis/genetics ; Cellular Reprogramming ; Cellular Reprogramming Techniques/methods ; Electroporation/methods ; Genetic Vectors/genetics ; Humans ; Induced Pluripotent Stem Cells/metabolism ; Introns ; MicroRNAs/genetics ; Transfection/methods
    Chemical Substances MIRN302A microRNA, human ; MicroRNAs
    Language English
    Publishing date 2020-01-31
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0290-4_12
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: The microRNA and the perspectives of miR-302.

    Chen, Emily Yen Yu / Chen, Jack S / Ying, Shao-Yao

    Heliyon

    2019  Volume 5, Issue 1, Page(s) e01167

    Abstract: MiRNAs are naturally occurring, small, non-coding RNA molecules that post-transcriptionally regulate the expression of a large number of genes involved in various biological processes, either through mRNA degradation or through translation inhibition. ... ...

    Abstract MiRNAs are naturally occurring, small, non-coding RNA molecules that post-transcriptionally regulate the expression of a large number of genes involved in various biological processes, either through mRNA degradation or through translation inhibition. MiRNAs play important roles in many aspects of physiology and pathology throughout the body, particularly in cancer, which have made miRNAs attractive tools and targets for translational research. The types of non-coding RNAs, biogenesis of miRNAs, circulating miRNAs, and direct delivery of miRNA were briefly reviewed. As a case of point, the role and perspective of miR-302, a family of ES-specific miRNA, on cancer, iPSCs, heart disease were presented.
    Language English
    Publishing date 2019-01-25
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2019.e01167
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR-302 Induction.

    Lin, Shi-Lung / Ying, Shao-Yao

    Methods in molecular biology (Clifton, N.J.)

    2017  Volume 1733, Page(s) 265–282

    Abstract: Today's researchers generating induced pluripotent stem cells (iPS cells or iPSCs) usually consider their pluripotency rather than potential tumorigenicity. Oncogenic factors such as c-Myc and Klf4 are frequently used to boost the survival and ... ...

    Abstract Today's researchers generating induced pluripotent stem cells (iPS cells or iPSCs) usually consider their pluripotency rather than potential tumorigenicity. Oncogenic factors such as c-Myc and Klf4 are frequently used to boost the survival and proliferative rates of iPSCs, creating an inevitable problem of tumorigenicity that hinders the therapeutic usefulness of these iPSCs. To prevent stem cell tumorigenicity, we have examined mechanisms by which the cell cycle genes are regulated in embryonic stem cells (ESCs). Naturally, ESCs possess two unique stemness properties: pluripotent differentiation into almost all cell types and unlimited self-renewal without the risk of tumor formation. These two features are also important for the use of ESCs or iPSCs in therapy. Currently, despite overwhelming reports describing iPSC pluripotency, there is no report of any tumor prevention mechanism in either ESCs or iPSCs. To this, our studies have revealed for the first time that an ESC-specific microRNA (miRNA), miR-302, regulates human iPSC tumorigenicity through cosuppression of both cyclin E-CDK2 and cyclin D-CDK4/6 cell cycle pathways during G1-S phase transition. Moreover, miR-302 also silences BMI-1, a cancer stem cell gene marker, to promote the expression of two senescence-associated tumor suppressor genes, p16Ink4a and p14/p19Arf. Together, the combinatory effects of inhibiting G1-S cell cycle transition and increasing p16/p14(p19) expression result in an attenuated cell cycle rate similar to that of 2-to-8-cell-stage embryonic cells in early zygotes (20-24 h/cycle), which is however slower than the fast proliferation rate of iPSCs induced by the four defined factors Oct4-Sox2-Klf4-c-Myc (12-16 h/cycle). These findings provide a means to control iPSC tumorigenicity and improve the safety of iPSCs for the therapeutic use. In this chapter, we review the mechanism underlying miR-302-mediated tumor suppression and then demonstrate how to apply this mechanism to generate tumor-free iPSCs. The same strategy may also be used to prevent ESC tumorigenicity.
    MeSH term(s) Animals ; Biomarkers ; Cellular Reprogramming/genetics ; Embryonic Stem Cells/metabolism ; Gene Expression Regulation, Developmental ; Gene Silencing ; Genes, Reporter ; Genetic Vectors ; Humans ; Induced Pluripotent Stem Cells/metabolism ; Introns ; MicroRNAs/genetics ; Models, Biological ; Multigene Family ; Rats ; Transfection
    Chemical Substances Biomarkers ; MicroRNAs
    Language English
    Publishing date 2017-12-20
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7601-0_22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs.

    Lin, Shi-Lung / Ying, Shao-Yao

    Methods in molecular biology (Clifton, N.J.)

    2017  Volume 1733, Page(s) 107–126

    Abstract: MicroRNAs (miRNAs), small single-stranded regulatory RNAs capable of interfering with intracellular messenger RNAs (mRNAs) that contain either complete or partial complementarity, are useful for the design of new therapies against cancer polymorphism and ...

    Abstract MicroRNAs (miRNAs), small single-stranded regulatory RNAs capable of interfering with intracellular messenger RNAs (mRNAs) that contain either complete or partial complementarity, are useful for the design of new therapies against cancer polymorphism and viral mutation. Numerous miRNAs have been reported to induce RNA interference (RNAi), a post-transcriptional gene-silencing mechanism. Recent evidence also indicates that they are involved in the transcriptional regulation of genome activities. They were first discovered in Caenorhabditis elegans as native RNA fragments that modulate a wide range of genetic regulatory pathways during embryonic development, and are now recognized as small gene silencers transcribed from the noncoding regions of a genome. In humans, nearly 97% of the genome is noncoding DNA, which varies from one individual to another, and changes in these sequences are frequently noted to manifest in clinical and circumstantial malfunction; for example, type 2 myotonic dystrophy and fragile X syndrome were found to be associated with miRNAs derived from introns. Intronic miRNA is a new class of miRNAs derived from the processing of non-protein-coding regions of gene transcripts. The intronic miRNAs differ uniquely from previously described intergenic miRNAs in the requirement of RNA polymerase (Pol)-II and spliceosomal components for its biogenesis. Several kinds of intronic miRNAs have been identified in C. elegans, mouse, and human cells; however, their functions and applications have not been reported. Here, we show for the first time that intron-derived miRNA is not only able to induce RNAi in mammalian cells but also in fish, chicken embryos, and adult mice cells, demonstrating the evolutionary preservation of this gene regulation system in vivo. These miRNA-mediated animal models provide artificial means to reproduce the mechanisms of miRNA-induced disease in vivo and will shed further light on miRNA-related therapies.
    MeSH term(s) Cloning, Molecular ; Gene Expression Regulation ; Gene Silencing ; Genes, Reporter ; Genetic Vectors/genetics ; Introns ; MicroRNAs/genetics ; RNA Interference ; RNA-Induced Silencing Complex ; Transfection
    Chemical Substances MicroRNAs ; RNA-Induced Silencing Complex
    Language English
    Publishing date 2017-12-20
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7601-0_9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Book: MicroRNA protocols

    Ying, Shao-Yao

    (Methods in molecular biology, ; 936 ; Springer protocols)

    2013  

    Author's details edited by Shao-Yao Ying
    Series title Methods in molecular biology, ; 936
    Springer protocols
    MeSH term(s) MicroRNAs
    Language English
    Size xi, 375 p. :, ill. (some col.)
    Edition 2nd ed.
    Publisher Humana Press ; Springer
    Publishing place New York
    Document type Book
    ISBN 9781627030823 ; 1627030824 ; 9781627030830 ; 1627030832
    Database Catalogue of the US National Library of Medicine (NLM)

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