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Book: Klar kann Alex schwimmen!

Umansky, Kaye / Chamberlain, Margaret / Trinker, Birgit

2002

Author's details	Kaye Umansky; Bilder von Margaret Chamberlain. [Aus dem Englischen von Birgit Trinker]
Keywords	Angst ; Bilderbuch ; Schwimmen
Size	32 Seiten
Publisher	Betz; Wien; München
Document type	Book
HBZ-ID	HT013273106
ISBN	3-219-10986-1 ; 978-3-219-10986-3
Database	Central Library of Sport Science of the German Sport University Cologne

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Article ; Online: Under the Skin - A Dermatologist's Fight to Save the NHS.

Chamberlain, Alex J

The Australasian journal of dermatology

2022 Volume 63, Issue 4, Page(s) 515–516

MeSH term(s)	Humans ; Dermatologists ; State Medicine ; Skin ; Dermatology ; Skin Diseases/diagnosis ; Skin Diseases/drug therapy
Language	English
Publishing date	2022-09-09
Publishing country	Australia
Document type	Letter
ZDB-ID	138052-7
ISSN	1440-0960 ; 0004-8380
ISSN (online)	1440-0960
ISSN	0004-8380
DOI	10.1111/ajd.13914
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Article ; Online: A systematic review of methodologies for studying behavioral imprinting

Chamberlain, Mac L. / Kacelnik, Alex / Bush, Autumn / Hauber, Mark E.

Ethology. 2024 Jan., v. 130, no. 1 p.e13416-

2024

Abstract: Behavioral imprinting is a learning phenomenon by which animals acquire preferences for stimuli through perceptual exposure during critical periods, without substantial external reinforcement. Since being acknowledged in 1516 by Sir Thomas More in ... ...

Abstract	Behavioral imprinting is a learning phenomenon by which animals acquire preferences for stimuli through perceptual exposure during critical periods, without substantial external reinforcement. Since being acknowledged in 1516 by Sir Thomas More in artificially incubated domestic chickens, imprinting has been reported in diverse species, across various sensory modalities, and during different life‐history stages. Due to this diversity, imprinting research uses highly varied methodologies, with distinctive differences between the methods employed for different types of imprinting. We systematically review relevant literature, identifying and describing the range of methodologies used to study imprinting across taxa and modalities. After compiling a representative dataset of 192 behavioral imprinting‐focused experiments, we categorize studies by imprinting sensory modality, focal species, ontogenetic stage addressed, and methods applied for both exposure and testing. The majority of studies in the sample focus on filial imprinting in precocial birds but nonfilial types, such as sexual or home range imprinting in altricial and non‐avian species, are also present, albeit at far lower proportions. Filial imprinting is studied across different sensory modalities, mostly through applying artificial stimuli, but nonfilial imprinting studies mainly use live animals as stimuli, without isolating the relevant sensory modalities. Most studies of filial imprinting measure preference by spatial proximity, following response, or the suppression of a fear response, whereas most studies of sexual imprinting employ the attempt frequency of sexual behaviors. Finally, we analyze the relative frequencies of methodological approaches in each imprinting category, to highlight potential biases due to uneven research effort rather than intrinsic biology. The patterns and biases in imprinting methodologies that we uncover hamper attempts to establish whether different forms of imprinting share mechanistic foundations, including whether imprinting constitutes a biologically meaningful learning category.
Keywords	animal behavior ; data collection ; fearfulness ; home range ; life history ; ontogeny ; systematic review
Language	English
Dates of publication	2024-01
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	REVIEW
ZDB-ID	633469-6
ISSN	0179-1613
ISSN	0179-1613
DOI	10.1111/eth.13416
Database	NAL-Catalogue (AGRICOLA)

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Article: Multiscale Fabrication Process Optimization of DFB Cavities for Organic Laser Diodes.

Ouirimi, Amani / Chime, Alex Chamberlain / Loganathan, Nixson / Chakaroun, Mahmoud / Gaimard, Quentin / Fischer, Alexis P A

Micromachines

2024 Volume 15, Issue 2

Abstract: In the context of the quest for the Organic Laser Diode, we present the multiscale fabrication process optimization of mixed-order distributed-feedback micro-cavities integrated in nanosecond-short electrical pulse-ready organic light-emitting diodes ( ... ...

Abstract	In the context of the quest for the Organic Laser Diode, we present the multiscale fabrication process optimization of mixed-order distributed-feedback micro-cavities integrated in nanosecond-short electrical pulse-ready organic light-emitting diodes (OLEDs). We combine ultra-short pulsed electrical excitation and laser micro-cavities. This requires the integration of a highly resolved DFB micro-cavity with an OLED stack and with microwave electrodes. In a second challenge, we tune the cavity resonance precisely to the electroluminescence peak of the organic laser gain medium. This requires precise micro-cavity fabrication performed using e-beam lithography to pattern gratings with a precision in the nanometer scale. Optimal DFB micro-cavities are obtained with 300 nm thick hydrogen silsesquioxane negative-tone e-beam resist on 50 nm thin indium tin oxide anode exposed with a charge quantity per area (i.e., dose) of 620 µC/cm
Language	English
Publishing date	2024-02-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2620864-7
ISSN	2072-666X
ISSN	2072-666X
DOI	10.3390/mi15020260
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Article ; Online: Translation initiation from sequence variants of the bacteriophage T7 g10RBS in Escherichia coli and Agrobacterium fabrum.

Benedict, Alex B / Chamberlain, Joshua D / Calvopina, Diana G / Griffitts, Joel S

Molecular biology reports

2021 Volume 49, Issue 1, Page(s) 833–838

Abstract: Background: The bacteriophage T7 gene 10 ribosome binding site (g10RBS) has long been used for robust expression of recombinant proteins in Escherichia coli. This RBS consists of a Shine-Dalgarno (SD) sequence augmented by an upstream translational " ... ...

Abstract	Background: The bacteriophage T7 gene 10 ribosome binding site (g10RBS) has long been used for robust expression of recombinant proteins in Escherichia coli. This RBS consists of a Shine-Dalgarno (SD) sequence augmented by an upstream translational "enhancer" (Enh) element, supporting protein production at many times the level seen with simple synthetic SD-containing sequences. The objective of this study was to dissect the g10RBS to identify simpler derivatives that exhibit much of the original translation efficiency. Methods and results: Twenty derivatives of g10RBS were tested using multiple promoter/reporter gene contexts. We have identified one derivative (which we call "CON_G") that maintains 100% activity in E. coli and is 33% shorter. Further minimization of CON_G results in variants that lose only modest amounts of activity. Certain nucleotide substitutions in the spacer region between the SD sequence and initiation codon show strong decreases in translation. When testing these 20 derivatives in the alphaproteobacterium Agrobacterium fabrum, most supported strong reporter protein expression that was not dependent on the Enh. Conclusions: The g10RBS derivatives tested in this study display a range of observed activity, including a minimized version (CON_G) that retains 100% activity in E. coli while being 33% shorter. This high activity is evident in two different promoter/reporter sequence contexts. The array of RBS sequences presented here may be useful to researchers in need of fine-tuned expression of recombinant proteins of interest.
MeSH term(s)	Agrobacterium/genetics ; Agrobacterium/metabolism ; Agrobacterium/virology ; Bacteriophage T7/genetics ; Binding Sites ; Codon, Initiator/genetics ; Enhancer Elements, Genetic/genetics ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli/virology ; Genes, Reporter ; Genetic Engineering/methods ; Plasmids ; Promoter Regions, Genetic/genetics ; Protein Biosynthesis/genetics ; Recombinant Proteins/metabolism ; Ribosomes/metabolism
Chemical Substances	Codon, Initiator ; Recombinant Proteins
Language	English
Publishing date	2021-11-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-021-06891-z
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ab Jg. 2022: Lesesaal (EG)

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Article: Translation initiation from sequence variants of the bacteriophage T7 g10RBS in Escherichia coli and Agrobacterium fabrum

Benedict, Alex B. / Chamberlain, Joshua D. / Calvopina, Diana G. / Griffitts, Joel S.

Molecular biology reports. 2022 Jan., v. 49, no. 1

2022

Abstract: BACKGROUND: The bacteriophage T7 gene 10 ribosome binding site (g10RBS) has long been used for robust expression of recombinant proteins in Escherichia coli. This RBS consists of a Shine–Dalgarno (SD) sequence augmented by an upstream translational “ ... ...

Abstract	BACKGROUND: The bacteriophage T7 gene 10 ribosome binding site (g10RBS) has long been used for robust expression of recombinant proteins in Escherichia coli. This RBS consists of a Shine–Dalgarno (SD) sequence augmented by an upstream translational “enhancer” (Enh) element, supporting protein production at many times the level seen with simple synthetic SD-containing sequences. The objective of this study was to dissect the g10RBS to identify simpler derivatives that exhibit much of the original translation efficiency. METHODS AND RESULTS: Twenty derivatives of g10RBS were tested using multiple promoter/reporter gene contexts. We have identified one derivative (which we call “CON_G”) that maintains 100% activity in E. coli and is 33% shorter. Further minimization of CON_G results in variants that lose only modest amounts of activity. Certain nucleotide substitutions in the spacer region between the SD sequence and initiation codon show strong decreases in translation. When testing these 20 derivatives in the alphaproteobacterium Agrobacterium fabrum, most supported strong reporter protein expression that was not dependent on the Enh. CONCLUSIONS: The g10RBS derivatives tested in this study display a range of observed activity, including a minimized version (CON_G) that retains 100% activity in E. coli while being 33% shorter. This high activity is evident in two different promoter/reporter sequence contexts. The array of RBS sequences presented here may be useful to researchers in need of fine-tuned expression of recombinant proteins of interest.
Keywords	Agrobacterium ; Escherichia coli ; bacteriophages ; molecular biology ; protein synthesis ; reporter genes ; ribosomes ; start codon
Language	English
Dates of publication	2022-01
Size	p. 833-838.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-021-06891-z
Database	NAL-Catalogue (AGRICOLA)

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Article: Investigating the Effects of Physical Therapy Timing, Intensity and Duration on Post-Traumatic Joint Contracture in a Rat Elbow Model.

Reiter, Alex J / Castile, Ryan M / Schott, Hayden R / Kivitz, Griffin J / Chamberlain, Aaron M / Lake, Spencer P

Muscles, ligaments and tendons journal

2022 Volume 11, Issue 3, Page(s) 547–553

Abstract: Background: Post-traumatic joint contracture (PTJC), characterized by loss of motion and permanent stiffness, affects up to 50% of patients following elbow joint dislocation or fracture. Mechanisms governing successful conservative treatment methods ... ...

Abstract	Background: Post-traumatic joint contracture (PTJC), characterized by loss of motion and permanent stiffness, affects up to 50% of patients following elbow joint dislocation or fracture. Mechanisms governing successful conservative treatment methods aimed at preventing elbow PTJC and avoiding operative treatments (e.g., physical therapy) are poorly understood. Using a previously established rat model of elbow PTJC, the purpose of this study was to explore the effect of varying timing, intensity and duration of active, functional exercise on joint motion outcomes. Methods: Following a surgically-induced unilateral elbow dislocation in rats, injured limbs were immobilized in bandages for 42 days followed by free mobilization for 42 additional days producing long-term PTJC. This work summarizes several studies (Phases I-III) that investigated the effects of early versus delayed therapy (timing), free mobilization versus forced treadmill walking (intensity), and limited-time versus unlimited use (duration) on elbow PTJC. Results: Joint motion outcomes in therapy groups showed no improvements compared to non-treated injured animals when therapy began day 14 post-injury or later regardless of timing, intensity or duration. Improved joint range-of-motion was only achieved when bandages were permanently removed at day 3 post-injury, regardless of whether added treadmill walking was performed. Conclusion: Early motion is essential to preserving range-of-motion following traumatic elbow injury in a rat model.
Language	English
Publishing date	2022-03-09
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2702144-0
ISSN	2240-4554
ISSN	2240-4554
DOI	10.32098/mltj.03.2021.20
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Article ; Online: Early Joint Use Following Elbow Dislocation Limits Range-of-Motion Loss and Tissue Pathology in Posttraumatic Joint Contracture.

Reiter, Alex J / Schott, Hayden R / Castile, Ryan M / Cannon, Paul C / Havlioglu, Necat / Chamberlain, Aaron M / Lake, Spencer P

The Journal of bone and joint surgery. American volume

2022 Volume 105, Issue 3, Page(s) 223–230

Abstract: Background: Simple elbow dislocation occurs at an incidence of 2.9 to 5.21 dislocations per 100,000 person-years, with as many as 62% of these patients experiencing long-term elbow joint contracture, stiffness, and/or pain. Poor outcomes and the need ... ...

Abstract	Background: Simple elbow dislocation occurs at an incidence of 2.9 to 5.21 dislocations per 100,000 person-years, with as many as 62% of these patients experiencing long-term elbow joint contracture, stiffness, and/or pain. Poor outcomes and the need for secondary surgical intervention can often be prevented nonoperatively with early or immediate active mobilization and physical therapy. However, immobilization or limited mobilization may be necessary following trauma, and it is unknown how different periods of immobilization affect pathological changes in elbow joint tissue and how these changes relate to range of motion (ROM). The purpose of this study was to investigate the effects of varying the initiation of free mobilization on elbow ROM and histological features in an animal model of elbow posttraumatic joint contracture. Methods: Traumatic elbow dislocation was surgically induced unilaterally in rats. Injured forelimbs were immobilized in bandages for 3, 7, 14, or 21 days; free mobilization was then allowed until 42 days after injury. Post-mortem joint ROM testing and histological analysis were performed. One-way analysis of variance was used to compare ROM data between control and injured groups, and Pearson correlations were performed between ROM parameters and histological outcomes. Results: Longer immobilization periods resulted in greater ROM reductions. The anterior and posterior capsule showed increases in cellularity, fibroblasts, adhesions, fibrosis, and thickness, whereas the measured outcomes in cartilage were mostly unaffected. All measured histological characteristics of the capsule were negatively correlated with ROM, indicating that higher degrees of pathology corresponded with less ROM. Conclusions: Longer immobilization periods resulted in greater ROM reductions, which correlated with worse histological outcomes in the capsule in an animal model of posttraumatic elbow contracture. The subtle differences in the timing of ROM and capsule tissue changes revealed in the present study provide new insight into the distinct timelines of biomechanical changes as well as regional tissue pathology. Clinical relevance: This study showed that beginning active mobilization 3 days after injury minimized posttraumatic joint contracture, thereby supporting an immediate-motion clinical treatment strategy (when possible). Furthermore, uninjured but pathologically altered periarticular tissues near the injury location may contribute to more severe contracture during longer immobilization periods as the disease state progresses.
MeSH term(s)	Rats ; Animals ; Elbow ; Joint Dislocations/complications ; Contracture/etiology ; Elbow Joint ; Physical Therapy Modalities ; Range of Motion, Articular
Language	English
Publishing date	2022-12-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	220625-0
ISSN	1535-1386 ; 0021-9355
ISSN (online)	1535-1386
ISSN	0021-9355
DOI	10.2106/JBJS.22.00064
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Article ; Online: Exploring the Reported Strengths and Limitations of Aboriginal and Torres Strait Islander Health Research: A Narrative Review of Intervention Studies.

McGuffog, Romany / Bryant, Jamie / Booth, Kade / Collis, Felicity / Brown, Alex / Hughes, Jaquelyne T / Chamberlain, Catherine / McGhie, Alexandra / Hobden, Breanne / Kennedy, Michelle

International journal of environmental research and public health

2023 Volume 20, Issue 5

Abstract: High quality intervention research is needed to inform evidence-based practice and policy for Aboriginal and Torres Strait Islander communities. We searched for studies published from 2008-2020 in the PubMed database. A narrative review of intervention ... ...

Abstract	High quality intervention research is needed to inform evidence-based practice and policy for Aboriginal and Torres Strait Islander communities. We searched for studies published from 2008-2020 in the PubMed database. A narrative review of intervention literature was conducted, where we identified researcher reported strengths and limitations of their research practice. A total of 240 studies met inclusion criteria which were categorised as evaluations, trials, pilot interventions or implementation studies. Reported strengths included community engagement and partnerships; sample qualities; Aboriginal and Torres Strait Islander involvement in research; culturally appropriate and safe research practice; capacity building efforts; providing resources or reducing costs for services and communities; understanding local culture and context; and appropriate timelines for completion. Reported limitations included difficulties achieving the target sample size; inadequate time; insufficient funding and resources; limited capacity of health workers and services; and inadequate community involvement and communication issues. This review highlights that community consultation and leadership coupled with appropriate time and funding, enables Aboriginal and Torres Strait Islander health intervention research to be conducted. These factors can enable effective intervention research, and consequently can help improve health and wellbeing outcomes for Aboriginal and Torres Strait Islander people.
MeSH term(s)	Humans ; Australian Aboriginal and Torres Strait Islander Peoples ; Health Workforce ; Health Services, Indigenous
Language	English
Publishing date	2023-02-23
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20053993
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Article: Dermoscopic Findings in Intraepidermal Carcinoma: an Interobserver Agreement Study.

Fougelberg, Julia / Luong, Alfred / Bowling, Jonathan / Chamberlain, Alex / Lallas, Aimilios / Marghoob, Ashfaq / Polesie, Sam / Salerni, Gabriel / Tanaka, Masaru / Zaar, Oscar / Zalaudek, Iris / Claeson, Magdalena / Paoli, John

Dermatology practical & conceptual

2023 Volume 13, Issue 1

Abstract: Introduction: A wide range of descriptive terms have been used for dermoscopic findings in intraepidermal carcinoma (IEC) and the clinical diagnostic accuracy of IEC can be challenging. Furthermore, dermoscopic findings in IEC have only rarely been ... ...

Abstract	Introduction: A wide range of descriptive terms have been used for dermoscopic findings in intraepidermal carcinoma (IEC) and the clinical diagnostic accuracy of IEC can be challenging. Furthermore, dermoscopic findings in IEC have only rarely been evaluated in fair-skinned populations. Objectives: To measure the interobserver agreement between dermatologists for dermoscopic findings in IEC. Furthermore, to describe the frequency of these findings in a predominantly fair-skinned population. Methods: One hundred dermoscopic images of histopathologically verified IECs were collected. The 11 most common dermoscopic findings described in previous studies were re-defined in a new terminology in a pre-study consensus meeting. Images were assessed by eight experienced international dermoscopists. The frequency of findings and the interobserver agreement was analyzed. Results: Scales (83%), dotted/glomerular vessels (77%), pinkish-white areas (73%) and hemorrhage (46%) were the most commonly present dermoscopic findings. Pigmented structures were found in 32% and shiny white structures (follicular or stromal) in 54% of the IEC. Vascular structures (vessels and/or hemorrhage) could be seen in 89% of the lesions. Overall, the interobserver agreement for the respective dermoscopic findings was poor to moderate, with the highest kappa values noted for scales (0.55) and hemorrhage (0.54) and the lowest for pinkish-white areas (0.015). Conclusion: Our results confirm those of previous studies on dermoscopy in IEC, including the frequency of pigmented structures despite the fair-skinned population. The interobserver agreement was relatively low. The proposed new terminology and our findings can hopefully serve as a guideline for researchers, teachers and students on how to identify IEC.
Language	English
Publishing date	2023-01-01
Publishing country	Austria
Document type	Journal Article
ZDB-ID	2685397-8
ISSN	2160-9381
ISSN	2160-9381
DOI	10.5826/dpc.1301a114
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