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Article ; Online: Direct RNA sequencing reveals m

Price, Alexander M / Hayer, Katharina E / McIntyre, Alexa B R / Gokhale, Nandan S / Abebe, Jonathan S / Della Fera, Ashley N / Mason, Christopher E / Horner, Stacy M / Wilson, Angus C / Depledge, Daniel P / Weitzman, Matthew D

Nature communications

2020 Volume 11, Issue 1, Page(s) 6016

Abstract: ... methyladenosine (m ...

Abstract	Adenovirus is a nuclear replicating DNA virus reliant on host RNA processing machinery. Processing and metabolism of cellular RNAs can be regulated by METTL3, which catalyzes the addition of N6-methyladenosine (m
MeSH term(s)	A549 Cells ; Adenosine/analogs & derivatives ; Adenosine/metabolism ; Adenovirus Infections, Human/virology ; Adenoviruses, Human/genetics ; Adenoviruses, Human/pathogenicity ; DNA, Viral/genetics ; Gene Knockdown Techniques ; Gene Knockout Techniques ; HEK293 Cells ; Host-Pathogen Interactions/genetics ; Humans ; Methyltransferases/genetics ; Methyltransferases/metabolism ; RNA Splicing ; RNA, Small Interfering/metabolism ; RNA, Viral/genetics ; RNA, Viral/metabolism ; Sequence Analysis, RNA ; Virus Replication
Chemical Substances	DNA, Viral ; RNA, Small Interfering ; RNA, Viral ; N-methyladenosine (CLE6G00625) ; Methyltransferases (EC 2.1.1.-) ; METTL3 protein, human (EC 2.1.1.62) ; Adenosine (K72T3FS567)
Language	English
Publishing date	2020-11-26
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-020-19787-6
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Article ; Online: An integrated metabolomic and proteomic approach for the identification of covalent inhibitors of the main protease (M

Baron, Giovanna / Borella, Sofia / Della Vedova, Larissa / Vittorio, Serena / Vistoli, Giulio / Carini, Marina / Aldini, Giancarlo / Altomare, Alessandra

Talanta

2022 Volume 252, Page(s) 123824

Abstract: M ...

Abstract	M
MeSH term(s)	Animals ; SARS-CoV-2 ; Coronavirus 3C Proteases ; Peptide Hydrolases ; Proteomics ; Protease Inhibitors/pharmacology ; Protease Inhibitors/chemistry ; Protease Inhibitors/metabolism ; Molecular Docking Simulation ; Complex Mixtures ; Antiviral Agents/pharmacology ; Antiviral Agents/chemistry ; COVID-19 Drug Treatment
Chemical Substances	Coronavirus 3C Proteases (EC 3.4.22.28) ; Peptide Hydrolases (EC 3.4.-) ; Protease Inhibitors ; Complex Mixtures ; Antiviral Agents
Language	English
Publishing date	2022-08-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2022.123824
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Article ; Online: Novel M. tuberculosis specific IL-2 ELISpot assay discriminates adult patients with active or latent tuberculosis.

Della Bella, Chiara / Spinicci, Michele / Grassi, Alessia / Bartalesi, Filippo / Benagiano, Marisa / Truthmann, Katja / Tapinassi, Simona / Troilo, Arianna / D'Elios, Sofia / Alnwaisri, Heba / Shuralev, Eduard / Singh, Mahavir / Bartoloni, Alessandro / D'Elios, Mario Milco

PloS one

2018 Volume 13, Issue 6, Page(s) e0197825

Abstract: ... cases in 2016. Only 5-15% of people infected with M. tuberculosis develop TB disease while others remain ... response to M. tuberculosis antigens ESAT-6, CFP-10 and Ala-DH. Statistical analysis was performed ...

Abstract	Background: Tuberculosis (TB) still is a major worldwide health problem, with 10.4 million new cases in 2016. Only 5-15% of people infected with M. tuberculosis develop TB disease while others remain latently infected (LTBI) during their lifetime. Thus, the absence of tests able to distinguish between latent infection and active tuberculosis is one of the major limits of currently available diagnostic tools. Methods: A total of 215 patients were included in the study as active TB cases (n = 73), LTBI subjects (n = 88) and healthy persons (n = 54). Peripheral blood mononuclear cells (PBMCs) were isolated from each patient and the LIOSpot® TB anti-human IL-2 ELISpot assay was performed to test their proliferative response to M. tuberculosis antigens ESAT-6, CFP-10 and Ala-DH. Statistical analysis was performed to define the sensitivity and the specificity of the LIOSpot® TB kit for each antigen used and to set the best cut off value that enables discrimination between subjects with active TB or latent TB infection. Results: Comparing the LIOSpot® TB results for each tested antigen between uninfected and infected subjects and between people with latent infection and active TB disease, the differences were significant for each antigen (p< 0.0001) but the ROC analysis demonstrated a high accuracy for the Ala-DH test only, with a cut off value of 12.5 SFC per million PBMCs and the Ala-DH ROC curve conferred a 96% sensitivity and 100% specificity to the test. For the ESAT-6 antigen, with a best cut off value of 71.25 SFC per million PBMCs, a sensitivity of 86% and specificity of 36% was obtained. Finally, the best cut off value for CFP-10 was 231.25 SFC per million PBMCs, with a sensitivity of 80% and a specificity of 54%. Thus, as for IGRA assays such as Quantiferon and T-Spot TB tests, ESAT-6 and CFP-10 are unable to distinguish LTBI from active TB when IL-2 is measured. On the contrary, the IL-2 production induced by Ala-DH, measured by LIOSpot® TB kit, shows high sensitivity and specificity for active TB disease. Conclusions: This study demonstrates that the LIOSpot® TB test is a highly useful diagnostic tool to discriminate between latent TB infection and active tuberculosis in adults patients.
MeSH term(s)	Adult ; Aged ; Case-Control Studies ; Diagnosis, Differential ; Female ; Humans ; Immunoassay/methods ; Interleukin-2/immunology ; Latent Tuberculosis/diagnosis ; Male ; Middle Aged ; Mycobacterium tuberculosis/immunology ; ROC Curve ; Species Specificity
Chemical Substances	Interleukin-2
Language	English
Publishing date	2018-06-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0197825
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Article ; Online: Pola Bakteri Pasien Rinosinusitis Kronik di RSUP Dr. M. Djamil Padang 2016-2017

Della Reyhani Putri / Dolly Irfandi / Novita Ariani

Jurnal Ilmu Kesehatan Indonesia, Vol 1, Iss 2, Pp 36-

2020 Volume 42

Abstract: ... bakteri dan tes sensitivitas pada pasien rinosinusitis kronis polip dan non polip di RSUP Dr. M. Djamil ... dengan jumlah sampel 100 pasien RSK di RSUP Dr. M. Djamil Padang. Penelitian ini dilakukan dari bulan ...

Abstract	Latar Belakang. Rinosinusitis kronik (RSK) adalah peradangan mukosa yang melapisi hidung dan sinus paranasal lebih dari 12 minggu. RSK merupakan salah satu masalah kesehatan yang sering kita temukan di kehidupan masyarakat, penyakit ini dapat menurunkan kualitas hidup. Dalam pengobatan RSK, peranan antibiotik penting. Pola bakteri dan kepekaannya terhadap terapi antibiotik dapat berubah karena banyaknya bakteri yang resisten terhadap antibiotika tertentu. Objektif. Penelitian ini bertujuan untuk mengetahui pola bakteri dan tes sensitivitas pada pasien rinosinusitis kronis polip dan non polip di RSUP Dr. M. Djamil Padang periode Januari 2016-Desember 2017. Metode. Penelitian ini merupakan deskriptif retrospektif dengan jumlah sampel 100 pasien RSK di RSUP Dr. M. Djamil Padang. Penelitian ini dilakukan dari bulan November-Desember 2018. Teknik pengambilan sampel adalah total sampling selama tahun 2016-2017. Hasil. Prevalensi rinosinusitis kronik dengan polip lebih tinggi dibandingkan dengan rinosinusitis kronik non polip. Jenis bakteri yang ditemukan dengan persentase tertinggi pada RSK polip dan non polip adalah Staphylococcus aureus. Pada Rinosinusitis kronik, usia 41-50 memiliki prevalensi tertinggi yaitu 31 pasien. Kesimpulan. Sebagian besar jenis bakteri yang ditemukan resisten terhadap Ampicillin, dan sensitif terhadap Meropenem, Cefoperazone, dan Gentamisin. Usaha promotif dan preventif terhadap faktor risiko seperti merokok, polutan, dan lain-lain, perlu dilakukan karena prevalensi RSK yang tinggi pada kelompok usia tersebut. Kata kunci: rinosinusitis kronik polip dan non polip, pola bakteri, sensitivitas antibiotik.
Keywords	rinosinusitis ; polip ; antibiotik ; Medicine (General) ; R5-920
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	Fakultas Kedokteran Universitas Andalas
Document type	Article ; Online
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Article ; Online: Oil production by the marine microalgae Nannochloropsis sp. F&M-M24 and Tetraselmis suecica F&M-M33.

Bondioli, Paolo / Della Bella, Laura / Rivolta, Gabriele / Chini Zittelli, Graziella / Bassi, Niccolò / Rodolfi, Liliana / Casini, David / Prussi, Matteo / Chiaramonti, David / Tredici, Mario R

Bioresource technology

2012 Volume 114, Page(s) 567–572

Abstract: Nannochloropsis sp. F&M-M24 and Tetraselmis suecica F&M-M33 were cultivated outdoors in Green Wall ... attained average biomass and lipid productivities of 9.9 and 6.5 g m(-2) day(-1), respectively. Starved ... Tetraselmis cultures achieved a biomass productivity of about 7.6 g m(-2) day(-1) and a lipid productivity ...

Abstract	Nannochloropsis sp. F&M-M24 and Tetraselmis suecica F&M-M33 were cultivated outdoors in Green Wall Panels under nutrient deficiency to stimulate oil synthesis. Under nitrogen deprivation, Nannochloropsis attained average biomass and lipid productivities of 9.9 and 6.5 g m(-2) day(-1), respectively. Starved Tetraselmis cultures achieved a biomass productivity of about 7.6 g m(-2) day(-1) and a lipid productivity of 1.7 g m(-2) day(-1). Lipids represented 39.1% and 68.5% of non-starved and starved Nannochloropsis biomass, respectively. Starvation did not increase lipid content in Tetraselmis biomass. Important differences in lipid classes and in fatty acid composition were observed under the different cultivation conditions for both microalgae.
MeSH term(s)	Aquatic Organisms/microbiology ; Lipid Metabolism/physiology ; Microalgae/classification ; Microalgae/metabolism ; Oils/metabolism ; Species Specificity ; Water Microbiology
Chemical Substances	Oils
Language	English
Publishing date	2012-06
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2012.02.123
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Article: Electrical Conductivity of o-, m-, and p-Terphenyls

Bizzarri, P. Costa / C. Della Casa / S. Pietra

Zeitschrift für Naturforschung. 2014 June 2, v. 28, no. 5-6

2014

Abstract: ... as far as we know, only one on m-terphenyl¹⁰. In the present work, the d. c. electrical conductivities ...

Abstract	Many investigations have been carried out on the electrical properties of p-terphenyl¹⁻⁹, and, as far as we know, only one on m-terphenyl¹⁰. In the present work, the d. c. electrical conductivities of the three isomeric terphenyls are compared to establish the influence of the molecular structure on the electrical properties and to explain the mechanism of the energy transport in organic molecules.
Keywords	chemical compounds ; chemical reactions ; chemical structure ; electrical conductivity ; energy transfer
Language	English
Dates of publication	2014-0602
Size	p. 331-333.
Publishing place	Verlag der Zeitschrift für Naturforschung
Document type	Article
ZDB-ID	124635-5
ISSN	0340-5087 ; 0044-3174 ; 0932-0776 ; 0341-0447 ; 0341-0420
ISSN	0340-5087 ; 0044-3174 ; 0932-0776 ; 0341-0447 ; 0341-0420
DOI	10.1515/znb-1973-5-621
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Article ; Online: Bright radio emission from an ultraluminous stellar-mass microquasar in M 31.

Middleton, Matthew J / Miller-Jones, James C A / Markoff, Sera / Fender, Rob / Henze, Martin / Hurley-Walker, Natasha / Scaife, Anna M M / Roberts, Timothy P / Walton, Dominic / Carpenter, John / Macquart, Jean-Pierre / Bower, Geoffrey C / Gurwell, Mark / Pietsch, Wolfgang / Haberl, Frank / Harris, Jonathan / Daniel, Michael / Miah, Junayd / Done, Chris /

Morgan, John S / Dickinson, Hugh / Charles, Phil / Burwitz, Vadim / Della Valle, Massimo / Freyberg, Michael / Greiner, Jochen / Hernanz, Margarita / Hartmann, Dieter H / Hatzidimitriou, Despina / Riffeser, Arno / Sala, Gloria / Seitz, Stella / Reig, Pablo / Rau, Arne / Orio, Marina / Titterington, David / Grainge, Keith

Nature

2013 Volume 493, Issue 7431, Page(s) 187–190

Abstract: ... observations of a bright new X-ray source in the nearby galaxy M 31, whose peak luminosity exceeded 10(39) erg ...

Abstract	A subset of ultraluminous X-ray sources (those with luminosities of less than 10(40) erg s(-1); ref. 1) are thought to be powered by the accretion of gas onto black holes with masses of ∼5-20M cicled dot, probably by means of an accretion disk. The X-ray and radio emission are coupled in such Galactic sources; the radio emission originates in a relativistic jet thought to be launched from the innermost regions near the black hole, with the most powerful emission occurring when the rate of infalling matter approaches a theoretical maximum (the Eddington limit). Only four such maximal sources are known in the Milky Way, and the absorption of soft X-rays in the interstellar medium hinders the determination of the causal sequence of events that leads to the ejection of the jet. Here we report radio and X-ray observations of a bright new X-ray source in the nearby galaxy M 31, whose peak luminosity exceeded 10(39) erg s(-1). The radio luminosity is extremely high and shows variability on a timescale of tens of minutes, arguing that the source is highly compact and powered by accretion close to the Eddington limit onto a black hole of stellar mass. Continued radio and X-ray monitoring of such sources should reveal the causal relationship between the accretion flow and the powerful jet emission.
Language	English
Publishing date	2013-01-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/nature11697
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Article ; Online: Novel M. tuberculosis specific IL-2 ELISpot assay discriminates adult patients with active or latent tuberculosis.

Chiara Della Bella / Michele Spinicci / Alessia Grassi / Filippo Bartalesi / Marisa Benagiano / Katja Truthmann / Simona Tapinassi / Arianna Troilo / Sofia D'Elios / Heba Alnwaisri / Eduard Shuralev / Mahavir Singh / Alessandro Bartoloni / Mario Milco D'Elios

PLoS ONE, Vol 13, Iss 6, p e

2018 Volume 0197825

Abstract: ... in 2016. Only 5-15% of people infected with M. tuberculosis develop TB disease while others remain ... response to M. tuberculosis antigens ESAT-6, CFP-10 and Ala-DH. Statistical analysis was performed ...

Abstract	BACKGROUND:Tuberculosis (TB) still is a major worldwide health problem, with 10.4 million new cases in 2016. Only 5-15% of people infected with M. tuberculosis develop TB disease while others remain latently infected (LTBI) during their lifetime. Thus, the absence of tests able to distinguish between latent infection and active tuberculosis is one of the major limits of currently available diagnostic tools. METHODS:A total of 215 patients were included in the study as active TB cases (n = 73), LTBI subjects (n = 88) and healthy persons (n = 54). Peripheral blood mononuclear cells (PBMCs) were isolated from each patient and the LIOSpot® TB anti-human IL-2 ELISpot assay was performed to test their proliferative response to M. tuberculosis antigens ESAT-6, CFP-10 and Ala-DH. Statistical analysis was performed to define the sensitivity and the specificity of the LIOSpot® TB kit for each antigen used and to set the best cut off value that enables discrimination between subjects with active TB or latent TB infection. RESULTS:Comparing the LIOSpot® TB results for each tested antigen between uninfected and infected subjects and between people with latent infection and active TB disease, the differences were significant for each antigen (p< 0.0001) but the ROC analysis demonstrated a high accuracy for the Ala-DH test only, with a cut off value of 12.5 SFC per million PBMCs and the Ala-DH ROC curve conferred a 96% sensitivity and 100% specificity to the test. For the ESAT-6 antigen, with a best cut off value of 71.25 SFC per million PBMCs, a sensitivity of 86% and specificity of 36% was obtained. Finally, the best cut off value for CFP-10 was 231.25 SFC per million PBMCs, with a sensitivity of 80% and a specificity of 54%. Thus, as for IGRA assays such as Quantiferon and T-Spot TB tests, ESAT-6 and CFP-10 are unable to distinguish LTBI from active TB when IL-2 is measured. On the contrary, the IL-2 production induced by Ala-DH, measured by LIOSpot® TB kit, shows high sensitivity and specificity for active TB disease. ...
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article: Oil production by the marine microalgae Nannochloropsis sp. F&M-M24 and Tetraselmis suecica F&M-M33

Bondioli, Paolo / Della Bella, Laura / Rivolta, Gabriele / Chini Zittelli, Graziella / Bassi, Niccolò / Rodolfi, Liliana / Casini, David / Prussi, Matteo / Chiaramonti, David / Tredici, Mario R

Bioresource technology. 2012 June, v. 114

2012

Abstract: Nannochloropsis sp. F&M-M24 and Tetraselmis suecica F&M-M33 were cultivated outdoors in Green Wall ...

Abstract	Nannochloropsis sp. F&M-M24 and Tetraselmis suecica F&M-M33 were cultivated outdoors in Green Wall Panels under nutrient deficiency to stimulate oil synthesis. Under nitrogen deprivation, Nannochloropsis attained average biomass and lipid productivities of 9.9 and 6.5gm−2day−1, respectively. Starved Tetraselmis cultures achieved a biomass productivity of about 7.6gm−2day−1 and a lipid productivity of 1.7gm−2day−1. Lipids represented 39.1% and 68.5% of non-starved and starved Nannochloropsis biomass, respectively. Starvation did not increase lipid content in Tetraselmis biomass. Important differences in lipid classes and in fatty acid composition were observed under the different cultivation conditions for both microalgae.
Keywords	Tetraselmis suecica ; biomass ; fatty acid composition ; lipid content ; lipids ; microalgae ; nitrogen ; nutrient deficiencies ; oils ; starvation
Language	English
Dates of publication	2012-06
Size	p. 567-572.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2012.02.123
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: VATA-m: Visual-Analogue Test assessing Anosognosia for motor impairment.

Della Sala, S / Cocchini, G / Beschin, N / Cameron, A

The Clinical neuropsychologist

2009 Volume 23, Issue 3, Page(s) 406–427

Abstract: ... to develop a new tool, the VATA-m (Visual-Analogue Test for Anosognosia for motor impairment), to assess ... explicit anosognosia for motor impairments. The VATA-m is a questionnaire that compares a patient's ...

Abstract	There has been a growing interest in anosognosia in both clinical and research domains, yet relatively little attention has been paid to methods for evaluating it. Usually, the presence and severity of anosognosia is assessed by means of structured interviews or questionnaires. Both interviews and questionnaires can provide valuable information, but they rely heavily on self-evaluation and language, and are therefore prone to bias and pose more difficulty in the assessment of aphasic patients. The aim of this study was to develop a new tool, the VATA-m (Visual-Analogue Test for Anosognosia for motor impairment), to assess explicit anosognosia for motor impairments. The VATA-m is a questionnaire that compares a patient's self-evaluation with a caregivers' evaluation of the patient's abilities on a series of motor tasks. In addition, the test overcomes some of the limitations of the existing structured interviews and questionnaires, by enhancing reliability, improving data interpretation and diagnosis, and enabling assessment of patients with aphasia.
MeSH term(s)	Aged ; Agnosia/diagnosis ; Agnosia/physiopathology ; Female ; Humans ; Male ; Middle Aged ; Motor Skills ; Movement Disorders/diagnosis ; Movement Disorders/physiopathology ; Neuropsychological Tests/standards ; Reproducibility of Results ; Severity of Illness Index ; Surveys and Questionnaires ; Visual Perception
Language	English
Publishing date	2009-02-11
Publishing country	England
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639080-8
ISSN	1744-4144 ; 0920-1637 ; 1385-4046
ISSN (online)	1744-4144
ISSN	0920-1637 ; 1385-4046
DOI	10.1080/13854040802251393
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