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  1. Article ; Online: Bacterial Outer Membrane Vesicles as Antibiotic Delivery Vehicles.

    Collins, Shannon M / Brown, Angela C

    Frontiers in immunology

    2021  Volume 12, Page(s) 733064

    Abstract: Bacterial outer membrane vesicles (OMVs) are nanometer-scale, spherical vehicles released by Gram-negative bacteria into their surroundings throughout growth. These OMVs have been demonstrated to play key roles in pathogenesis by delivering certain ... ...

    Abstract Bacterial outer membrane vesicles (OMVs) are nanometer-scale, spherical vehicles released by Gram-negative bacteria into their surroundings throughout growth. These OMVs have been demonstrated to play key roles in pathogenesis by delivering certain biomolecules to host cells, including toxins and other virulence factors. In addition, this biomolecular delivery function enables OMVs to facilitate intra-bacterial communication processes, such as quorum sensing and horizontal gene transfer. The unique ability of OMVs to deliver large biomolecules across the complex Gram-negative cell envelope has inspired the use of OMVs as antibiotic delivery vehicles to overcome transport limitations. In this review, we describe the advantages, applications, and biotechnological challenges of using OMVs as antibiotic delivery vehicles, studying both natural and engineered antibiotic applications of OMVs. We argue that OMVs hold great promise as antibiotic delivery vehicles, an urgently needed application to combat the growing threat of antibiotic resistance.
    MeSH term(s) Animals ; Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/metabolism ; Bacterial Outer Membrane/metabolism ; Drug Carriers ; Drug Compounding ; Extracellular Vesicles/genetics ; Extracellular Vesicles/metabolism ; Gram-Negative Aerobic Bacteria/genetics ; Gram-Negative Aerobic Bacteria/metabolism ; Humans
    Chemical Substances Anti-Bacterial Agents ; Drug Carriers
    Language English
    Publishing date 2021-09-20
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.733064
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A systematic meta-epidemiologic review on nonabstinence-inclusive interventions for substance use: inclusion of race/ethnicity and sex assigned at birth/gender.

    Goldstein, Silvi C / Newberger, Noam G / Schick, Melissa R / Ferguson, Jewelia J / Collins, Susan E / Haeny, Angela M / Weiss, Nicole H

    The American journal of drug and alcohol abuse

    2024  , Page(s) 1–15

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2024-02-27
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 193086-2
    ISSN 1097-9891 ; 0095-2990
    ISSN (online) 1097-9891
    ISSN 0095-2990
    DOI 10.1080/00952990.2024.2308087
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1137: Show issues Location:
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  3. Article ; Online: Heterogeneity of Size and Toxin Distribution in

    Nice, Justin B / Collins, Shannon M / Agro, Samuel M J / Sinani, Anxhela / Moros, Spencer D / Pasch, Leah M / Brown, Angela C

    Toxins

    2024  Volume 16, Issue 3

    Abstract: Aggregatibacter ... ...

    Abstract Aggregatibacter actinomycetemcomitans
    MeSH term(s) Aggregatibacter actinomycetemcomitans ; Toxins, Biological ; Cytosol ; Biological Transport ; Cell Movement
    Chemical Substances Toxins, Biological
    Language English
    Publishing date 2024-03-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins16030138
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing.

    Bonnycastle, Lori L / Swift, Amy J / Mansell, Erin C / Lee, Angela / Winnicki, Elizabeth / Li, Elizabeth S / Robertson, Catherine C / Parsons, Victoria A / Huynh, Trung / Krilow, Chad / Mohlke, Karen L / Erdos, Michael R / Narisu, Narisu / Collins, Francis S

    The CRISPR journal

    2024  Volume 7, Issue 1, Page(s) 53–67

    Abstract: We developed an efficient CRISPR prime editing protocol and generated isogenic-induced pluripotent stem cell (iPSC) lines carrying heterozygous or homozygous alleles for putatively causal single nucleotide variants at six type 2 diabetes loci ( ...

    Abstract We developed an efficient CRISPR prime editing protocol and generated isogenic-induced pluripotent stem cell (iPSC) lines carrying heterozygous or homozygous alleles for putatively causal single nucleotide variants at six type 2 diabetes loci (
    MeSH term(s) Humans ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Diabetes Mellitus, Type 2 ; Induced Pluripotent Stem Cells ; CRISPR-Cas Systems/genetics ; Gene Editing ; RNA, Guide, CRISPR-Cas Systems
    Chemical Substances RNA, Guide, CRISPR-Cas Systems
    Language English
    Publishing date 2024-02-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3017891-5
    ISSN 2573-1602 ; 2573-1599
    ISSN (online) 2573-1602
    ISSN 2573-1599
    DOI 10.1089/crispr.2023.0066
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 7195: Show issues Location:
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    Zs.MG 119: Show issues

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  5. Article ; Online: Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity.

    Collins, Shannon M / Nice, Justin B / Chang, En Hyung / Brown, Angela C

    Journal of visualized experiments : JoVE

    2021  , Issue 169

    Abstract: The cell wall of Gram-negative bacteria consists of an inner (cytoplasmic) and outer membrane (OM), separated by a thin peptidoglycan layer. Throughout growth, the outer membrane can bleb to form spherical outer membrane vesicles (OMVs). These OMVs are ... ...

    Abstract The cell wall of Gram-negative bacteria consists of an inner (cytoplasmic) and outer membrane (OM), separated by a thin peptidoglycan layer. Throughout growth, the outer membrane can bleb to form spherical outer membrane vesicles (OMVs). These OMVs are involved in numerous cellular functions including cargo delivery to host cells and communication with bacterial cells. Recently, the therapeutic potential of OMVs has begun to be explored, including their use as vaccines and drug delivery vehicles. Although OMVs are derived from the OM, it has long been appreciated that the lipid and protein cargo of the OMV differs, often significantly, from that of the OM. More recently, evidence that bacteria can release multiple types of OMVs has been discovered, and evidence exists that size can impact the mechanism of their uptake by host cells. However, studies in this area are limited by difficulties in efficiently separating the heterogeneously sized OMVs. Density gradient centrifugation (DGC) has traditionally been used for this purpose; however, this technique is time-consuming and difficult to scale-up. Size exclusion chromatography (SEC), on the other hand, is less cumbersome and lends itself to the necessary future scale-up for therapeutic use of OMVs. Here, we describe a SEC approach that enables reproducible separation of heterogeneously sized vesicles, using as a test case, OMVs produced by Aggregatibacter actinomycetemcomitans, which range in diameter from less than 150 nm to greater than 350 nm. We demonstrate separation of "large" (350 nm) OMVs and "small" (<150 nm) OMVs, verified by dynamic light scattering (DLS). We recommend SEC-based techniques over DGC-based techniques for separation of heterogeneously sized vesicles due to its ease of use, reproducibility (including user-to-user), and possibility for scale-up.
    MeSH term(s) Bacterial Outer Membrane Proteins/metabolism ; Chromatography, Gel/methods ; Genetic Heterogeneity ; Reproducibility of Results
    Chemical Substances Bacterial Outer Membrane Proteins
    Language English
    Publishing date 2021-03-31
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/62429
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Sequential, low-temperature aqueous synthesis of Ag-In-S/Zn quantum dots

    Ozdemir, Nur Koncuy / Cline, Joseph P / Sakizadeh, John / Collins, Shannon M / Brown, Angela C / McIntosh, Steven / Kiely, Christopher J / Snyder, Mark A

    Journal of materials chemistry. B

    2022  Volume 10, Issue 24, Page(s) 4529–4545

    Abstract: The development of high quality, non-toxic ( ...

    Abstract The development of high quality, non-toxic (
    MeSH term(s) Biomineralization ; Cations ; Indium/chemistry ; Quantum Dots/chemistry ; Sulfides/chemistry ; Temperature ; Water/chemistry ; Zinc/chemistry
    Chemical Substances Cations ; Sulfides ; Indium (045A6V3VFX) ; Water (059QF0KO0R) ; Zinc (J41CSQ7QDS)
    Language English
    Publishing date 2022-06-22
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2702241-9
    ISSN 2050-7518 ; 2050-750X
    ISSN (online) 2050-7518
    ISSN 2050-750X
    DOI 10.1039/d2tb00682k
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: The first report of the mobile colistin resistance gene,

    Kigen, Collins / Muraya, Angela / Wachira, James / Musila, Lillian

    Microbiology spectrum

    2024  Volume 12, Issue 2, Page(s) e0185523

    Abstract: This study describes the identification of ... ...

    Abstract This study describes the identification of the
    MeSH term(s) Animals ; Humans ; Colistin ; Enterobacter cloacae/genetics ; Kenya ; Anti-Bacterial Agents/pharmacology ; Plasmids ; Mutation ; Microbial Sensitivity Tests ; Drug Resistance, Bacterial/genetics
    Chemical Substances Colistin (Z67X93HJG1) ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-01-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.01855-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Isotope analysis of human dental calculus δ

    Chidimuro, Blessing / Mundorff, Amy / Speller, Camilla / Radini, Anita / Boudreault, Noémie / Lucas, Mary / Holst, Malin / Lamb, Angela / Collins, Matthew / Alexander, Michelle

    Rapid communications in mass spectrometry : RCM

    2022  Volume 36, Issue 11, Page(s) e9286

    Abstract: ... Multiprep device to measure carbon, we tested the potential of dental calculus carbonate to identify C ... carbonate as a new biomaterial to identify C ...

    Abstract Rationale: Dental calculus (mineralised dental plaque) is composed primarily of hydroxyapatite. We hypothesise that the carbonate component of dental calculus will reflect the isotopic composition of ingested simple carbohydrates. Therefore, dental calculus carbonates may be an indicator for sugar consumption, and an alternative to bone carbonate in isotopic palaeodiet studies.
    Methods: We utilised Fourier transform infrared attenuated total reflectance analysis to characterise the composition and crystallisation of bone and dental calculus before isotope analysis of carbonate. Using a Sercon 20-22 mass spectrometer coupled with a Sercon GSL sample preparation system and an IsoPrime 100 dual inlet mass spectrometer plus Multiprep device to measure carbon, we tested the potential of dental calculus carbonate to identify C
    Results: The modern population shows higher δ
    Conclusions: Our results support dental calculus carbonate as a new biomaterial to identify C
    MeSH term(s) Carbon ; Carbon Isotopes/analysis ; Carbonates/analysis ; Dental Calculus ; Dietary Sugars ; Humans ; Isotopes/analysis ; Sugars
    Chemical Substances Carbon Isotopes ; Carbonates ; Dietary Sugars ; Isotopes ; Sugars ; Carbon (7440-44-0)
    Language English
    Publishing date 2022-03-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 58731-x
    ISSN 1097-0231 ; 0951-4198
    ISSN (online) 1097-0231
    ISSN 0951-4198
    DOI 10.1002/rcm.9286
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3461: Show issues Location:
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  9. Article: Size exclusion chromatography to analyze bacterial outer membrane vesicle heterogeneity

    Collins, Shannon M. / Nice, Justin B. / Chang, En Hyung / Brown, Angela C.

    Journal of visualized experiments. 2021 Mar. 31, , no. 169

    2021  

    Abstract: The cell wall of Gram-negative bacteria consists of an inner (cytoplasmic) and outer membrane (OM), separated by a thin peptidoglycan layer. Throughout growth, the outer membrane can bleb to form spherical outer membrane vesicles (OMVs). These OMVs are ... ...

    Abstract The cell wall of Gram-negative bacteria consists of an inner (cytoplasmic) and outer membrane (OM), separated by a thin peptidoglycan layer. Throughout growth, the outer membrane can bleb to form spherical outer membrane vesicles (OMVs). These OMVs are involved in numerous cellular functions including cargo delivery to host cells and communication with bacterial cells. Recently, the therapeutic potential of OMVs has begun to be explored, including their use as vaccines and drug delivery vehicles. Although OMVs are derived from the OM, it has long been appreciated that the lipid and protein cargo of the OMV differs, often significantly, from that of the OM. More recently, evidence that bacteria can release multiple types of OMVs has been discovered, and evidence exists that size can impact the mechanism of their uptake by host cells. However, studies in this area are limited by difficulties in efficiently separating the heterogeneously sized OMVs. Density gradient centrifugation (DGC) has traditionally been used for this purpose; however, this technique is time-consuming and difficult to scale-up. Size exclusion chromatography (SEC), on the other hand, is less cumbersome and lends itself to the necessary future scale-up for therapeutic use of OMVs. Here, we describe a SEC approach that enables reproducible separation of heterogeneously sized vesicles, using as a test case, OMVs produced by Aggregatibacter actinomycetemcomitans, which range in diameter from less than 150 nm to greater than 350 nm. We demonstrate separation of "large" (350 nm) OMVs and "small" (<150 nm) OMVs, verified by dynamic light scattering (DLS). We recommend SEC-based techniques over DGC-based techniques for separation of heterogeneously sized vesicles due to its ease of use, reproducibility (including user-to-user), and possibility for scale-up.
    Keywords Actinobacillus actinomycetemcomitans ; bacterial outer membranes ; cell walls ; density gradient centrifugation ; gel chromatography ; lipids ; peptidoglycans
    Language English
    Dates of publication 2021-0331
    Size p. e62429.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/62429
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Injury metrics are altered in spica-casted versus non-casted child ATDs in side-impact collisions with door intrusion.

    Mead, Matthew / Caskey, Sean / Walter, Norman / Atkinson, Patrick / Atkinson, Theresa / Collins, Angela C

    Traffic injury prevention

    2020  Volume 21, Issue 6, Page(s) 341–346

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Accidents, Traffic/statistics & numerical data ; Biomechanical Phenomena ; Child Restraint Systems ; Child, Preschool ; Humans ; Manikins ; Wounds and Injuries/epidemiology
    Language English
    Publishing date 2020-05-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2089818-6
    ISSN 1538-957X ; 1538-9588
    ISSN (online) 1538-957X
    ISSN 1538-9588
    DOI 10.1080/15389588.2020.1755969
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6253: Show issues Location:
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