Article ; Online: Distinctive Effects of GM-CSF and M-CSF on Proliferation and Polarization of Two Major Pulmonary Macrophage Populations.
Journal of immunology (Baltimore, Md. : 1950)
2019 Volume 202, Issue 9, Page(s) 2700–2709
Abstract: ... of AM functions throughout life, whereas M-CSF is broadly important for macrophage differentiation and ... self-renewal. However, the comparative actions of GM-CSF and M-CSF on AMs are incompletely understood ... their modulation by GM-CSF and M-CSF in murine primary AMs and IMs. CSFs increased the proliferation capacity and ...
| Abstract | GM-CSF is required for alveolar macrophage (AM) development shortly after birth and for maintenance of AM functions throughout life, whereas M-CSF is broadly important for macrophage differentiation and self-renewal. However, the comparative actions of GM-CSF and M-CSF on AMs are incompletely understood. Interstitial macrophages (IMs) constitute a second major pulmonary macrophage population. However, unlike AMs, IM responses to CSFs are largely unknown. Proliferation, phenotypic identity, and M1/M2 polarization are important attributes of all macrophage populations, and in this study, we compared their modulation by GM-CSF and M-CSF in murine primary AMs and IMs. CSFs increased the proliferation capacity and upregulated antiapoptotic gene expression in AMs but not IMs. GM-CSF, but not M-CSF, reinforced the cellular identity, as identified by surface markers, of both cell types. GM-CSF, but not M-CSF, increased the expression of both M1 and M2 markers exclusively in AMs. Finally, CSFs enhanced the IFN-γ- and IL-4-induced polarization ability of AMs but not IMs. These first (to our knowledge) data comparing effects on the two pulmonary macrophage populations demonstrate that the activating actions of GM-CSF and M-CSF on primary AMs are not conserved in primary IMs. |
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| MeSH term(s) | Animals ; Antigens, Differentiation/immunology ; Cell Proliferation/drug effects ; Granulocyte-Macrophage Colony-Stimulating Factor/immunology ; Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology ; Interferon-gamma/immunology ; Interleukin-4/immunology ; Macrophage Colony-Stimulating Factor/immunology ; Macrophage Colony-Stimulating Factor/pharmacology ; Macrophages, Alveolar/cytology ; Macrophages, Alveolar/immunology ; Male ; Mice |
| Chemical Substances | Antigens, Differentiation ; IFNG protein, mouse ; Il4 protein, mouse ; Interleukin-4 (207137-56-2) ; Macrophage Colony-Stimulating Factor (81627-83-0) ; Interferon-gamma (82115-62-6) ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1) |
| Language | English |
| Publishing date | 2019-03-13 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 3056-9 |
| ISSN | 1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381 |
| ISSN (online) | 1550-6606 |
| ISSN | 0022-1767 ; 1048-3233 ; 1047-7381 |
| DOI | 10.4049/jimmunol.1801387 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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