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  1. Article: An outreach activity to enhance biochemistry pedagogy.

    Roth, Karl S / Bannerman, Tammy / Gopalan, Venkat

    Trends in biochemical sciences

    2023  Volume 48, Issue 5, Page(s) 410–413

    Abstract: Students are self-motivated to learn when provided opportunities that connect theory and real-world applications. Here, we describe for biochemistry majors a newborn screening-focused outreach activity that seeks to develop students' mastery of ... ...

    Abstract Students are self-motivated to learn when provided opportunities that connect theory and real-world applications. Here, we describe for biochemistry majors a newborn screening-focused outreach activity that seeks to develop students' mastery of disciplinary content and soft skills (e.g., critical thinking, teamwork, effective communication, community engagement) and to enhance student engagement.
    MeSH term(s) Humans ; Biochemistry/education ; Students
    Language English
    Publishing date 2023-03-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 194216-5
    ISSN 1362-4326 ; 0968-0004 ; 0376-5067
    ISSN (online) 1362-4326
    ISSN 0968-0004 ; 0376-5067
    DOI 10.1016/j.tibs.2023.02.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Diabetes insipidus and Guillain-Barré-like syndrome following CAR-T cell therapy: a case report.

    Koch, Christian / Fleischer, Juliane / Popov, Todor / Frontzek, Karl / Schreiner, Bettina / Roth, Patrick / Manz, Markus G / Unseld, Simone / Müller, Antonia M S / Russkamp, Norman F

    Journal for immunotherapy of cancer

    2023  Volume 11, Issue 1

    Abstract: Background: Immune effector cell-associated neurotoxicity syndrome (ICANS) is a common adverse event of CD19-directed chimeric antigen receptor (CAR) T cell therapy. Other neurological adverse events, however, have not methodically been described and ... ...

    Abstract Background: Immune effector cell-associated neurotoxicity syndrome (ICANS) is a common adverse event of CD19-directed chimeric antigen receptor (CAR) T cell therapy. Other neurological adverse events, however, have not methodically been described and studied. Furthermore, safety data on CAR-T cell therapy in patients with central nervous system (CNS) lymphoma remain limited.
    Main body: We here report occurrence of a Guillain-Barré-like syndrome (GBS) and central diabetes insipidus (cDI) following tisagenlecleucel therapy for relapsed high-grade lymphoma with CNS involvement. Both complications were refractory to standard treatment of ICANS. Weakness of respiratory muscles required mechanical ventilation and tracheostomy while cDI was treated with desmopressin substitution for several weeks. Muscle-nerve biopsy and nerve conduction studies confirmed an axonal pattern of nerve damage. T cell-rich infiltrates and detection of the CAR transgene in muscle-nerve sections imply a direct or indirect role of CAR-T cell-mediated inflammation. In line with current treatment guidelines for GBS, intravenous immunoglobulin was administered and gradual but incomplete recovery was observed over the course of several months.
    Conclusions: This case report highlights the risk of rare but severe neurological adverse events, such as acute GBS or cDI, in patients treated with CAR-T cells. It further underlines the importance of appropriate patient surveillance and systematic reporting of rare complications to eventually improve treatment.
    MeSH term(s) Humans ; Receptors, Chimeric Antigen ; Immunotherapy, Adoptive/adverse effects ; Lymphoma, Non-Hodgkin ; Central Nervous System Neoplasms ; Diabetes Insipidus/etiology ; Cell- and Tissue-Based Therapy ; Diabetes Mellitus
    Chemical Substances Receptors, Chimeric Antigen ; cell-associated neurotoxicity
    Language English
    Publishing date 2023-01-23
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 2719863-7
    ISSN 2051-1426 ; 2051-1426
    ISSN (online) 2051-1426
    ISSN 2051-1426
    DOI 10.1136/jitc-2022-006059
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Nonspecific membrane bilayer perturbations by ivermectin underlie SARS-CoV-2

    Eastman, Richard T / Rusinova, Radda / Herold, Karl F / Huang, Xi-Ping / Dranchak, Patricia / Voss, Ty C / Rana, Sandeep / Shrimp, Jonathan H / White, Alex D / Hemmings, Hugh C / Roth, Bryan L / Inglese, James / Andersen, Olaf S / Dahlin, Jayme L

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Since it was proposed as a potential host-directed antiviral agent for SARS-CoV-2, the antiparasitic drug ivermectin has been investigated thoroughly in clinical trials, which have provided insufficient support for its clinical efficacy. To examine the ... ...

    Abstract Since it was proposed as a potential host-directed antiviral agent for SARS-CoV-2, the antiparasitic drug ivermectin has been investigated thoroughly in clinical trials, which have provided insufficient support for its clinical efficacy. To examine the potential for ivermectin to be repurposed as an antiviral agent, we therefore undertook a series of preclinical studies. Consistent with early reports, ivermectin decreased SARS-CoV-2 viral burden in
    Language English
    Publishing date 2023-10-24
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.10.23.563088
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book: Biochemistry and disease

    Cohn, Robert M. / Roth, Karl S.

    bridging basic science and clinical practice

    1996  

    Author's details Robert M. Cohn ; Karl S. Roth
    Keywords Medicine ; Biochemistry ; Metabolic Diseases
    Language English
    Size XIV, 587 S. : graph. Darst.
    Publisher Williams & Wilkins
    Publishing place Baltimore u.a.
    Publishing country United States
    Document type Book
    HBZ-ID HT007558033
    ISBN 0-683-02049-8 ; 978-0-683-02049-6
    Database Catalogue ZB MED Medicine, Health

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  5. Book: Pediatric endocrinology and inborn errors of metabolism

    Sarafoglou, Kyriakie / Hoffmann, Georg F / Roth, Karl S

    2017  

    Author's details editor, Kyriakie Sarafoglou ; associate editors, Georg F. Hoffmann, Karl S. Roth
    MeSH term(s) Endocrine System Diseases ; Child ; Metabolism, Inborn Errors ; Infant
    Language English
    Size p. ;, cm.
    Edition Second edition.
    Document type Book
    ISBN 9780071773140 ; 0071773142
    Database Catalogue of the US National Library of Medicine (NLM)

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  6. Article ; Online: Concurrent isolation of hepatic stem cells and hepatocytes from the human liver.

    Lee, Serene M L / Bertinetti-Lapatki, Cristina / Schiergens, Tobias S / Jauch, Karl-Walter / Roth, Adrian B / Thasler, Wolfgang E

    In vitro cellular & developmental biology. Animal

    2020  Volume 56, Issue 3, Page(s) 253–260

    Abstract: Hepatocytes differentiated from induced pluripotent stem cells or stem cells have the potential to be representative in vitro models of the human liver for research as well as early safety assessment programs. However, up until now, there has been no ... ...

    Abstract Hepatocytes differentiated from induced pluripotent stem cells or stem cells have the potential to be representative in vitro models of the human liver for research as well as early safety assessment programs. However, up until now, there has been no definitive proof that differentiated hepatocytes recapitulate the phenotype and functional characteristics of primary hepatocytes from the same individual. Thus, a method for the concurrent isolation of hepatocytes and hepatic stem cells is presented here to provide the cells necessary for the evaluation of the required benchmarking. The method presented here generated high-quality hepatocytes with a purity of 94 ± 1% and a high percentage viability of 79 ± 2%. Furthermore, the hepatic stem cells isolated were found to be actively proliferating and have a purity of 98 ± 1%. Thus, these isolated cells can be used as a powerful tool for the validation of differentiated hepatocyte in vitro models.
    MeSH term(s) Cell Separation/methods ; Epithelial Cell Adhesion Molecule/metabolism ; Hepatocytes/cytology ; Humans ; Keratin-19/metabolism ; Liver/cytology ; Liver Cirrhosis/pathology ; Stem Cells/cytology ; Stem Cells/metabolism
    Chemical Substances Epithelial Cell Adhesion Molecule ; Keratin-19
    Language English
    Publishing date 2020-03-27
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1077810-x
    ISSN 1543-706X ; 0883-8364 ; 1071-2690
    ISSN (online) 1543-706X
    ISSN 0883-8364 ; 1071-2690
    DOI 10.1007/s11626-020-00433-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: The immune response to a Coxiella burnetii vaccine in sheep varies according to their natural pre-exposure.

    Böttcher, Jens / Bauer, Benjamin U / Ambros, Christina / Alex, Michaela / Domes, Ursula / Roth, Sabine / Boll, Kerstin / Korneli, Martin / Bogner, Karl-Heinz / Randt, Andreas / Janowetz, Britta

    Vaccine

    2024  Volume 42, Issue 8, Page(s) 1993–2003

    Abstract: Q fever in humans is caused by Coxiella (C.) burnetii. In 2008 and 2012, cases of Q fever in humans were linked to an infected flock of approximately 650 ewes. Since 2013 gimmers (G'13, G'14, G'15 etc.) were primary vaccinated (two doses) with an ... ...

    Abstract Q fever in humans is caused by Coxiella (C.) burnetii. In 2008 and 2012, cases of Q fever in humans were linked to an infected flock of approximately 650 ewes. Since 2013 gimmers (G'13, G'14, G'15 etc.) were primary vaccinated (two doses) with an inactivated C.burnetii vaccine without any revaccination. In 2013, 30 ewes were primary vaccinated (A'13). Shedding was annually monitored by qPCR-testing of vaginal and nasal swabs collected at lambing. Animals were tested for Phase I- (PhI) and PhII-antibodies (Ab) and for PhII-specific-interferon-γ (IFN-γ) before and after vaccination. The effect of a revaccination was determined in 2018 and 2023. Groups of randomly selected gimmers primary vaccinated in 2015, 2016 and 2017 and a mixed group of older animals (A'13, G'13 and G'14) were revaccinated once in 2018. The trial was repeated in 2023 on groups primary vaccinated in 2019-2023. Major shedding after the outbreak in 2012 ceased in 2014. Thereafter C.burnetii was only sporadically detected at low-level in 2018, 2021 and 2023. Sheep naturally exposed to C.burnetii during the outbreak in 2012 (A'13, G'13) mounted a strong and complete (PhI, PhII, IFN-γ) recall immune response after vaccination. A serological PhI
    MeSH term(s) Humans ; Sheep ; Animals ; Female ; Coxiella burnetii ; Q Fever/prevention & control ; Q Fever/veterinary ; Q Fever/epidemiology ; Antibodies ; Bacterial Vaccines ; Immunity
    Chemical Substances Antibodies ; Bacterial Vaccines
    Language English
    Publishing date 2024-02-21
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2024.02.048
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Nonspecific membrane bilayer perturbations by ivermectin underlie SARS-CoV-2 in vitro activity

    Eastman, Richard T / Rusinova, Radda / Herold, Karl F / Huang, Xi-Ping / Dranchak, Patricia / Voss, Ty C / Rana, Sandeep / Shrimp, Jonathan H / White, Alex D / Hemmings, Hugh C / Roth, Bryan L / Inglese, James / Andersen, Olaf S / Dahlin, Jayme L

    bioRxiv

    Abstract: Since it was proposed as a potential host-directed antiviral agent for SARS-CoV-2, the antiparasitic drug ivermectin has been investigated thoroughly in clinical trials, which have provided insufficient support for its clinical efficacy. To examine the ... ...

    Abstract Since it was proposed as a potential host-directed antiviral agent for SARS-CoV-2, the antiparasitic drug ivermectin has been investigated thoroughly in clinical trials, which have provided insufficient support for its clinical efficacy. To examine the potential for ivermectin to be repurposed as an antiviral agent, we therefore undertook a series of preclinical studies. Consistent with early reports, ivermectin decreased SARS-CoV-2 viral burden in in vitro models at low micromolar concentrations, five- to ten-fold higher than the reported toxic clinical concentration. At similar concentrations, ivermectin also decreased cell viability and increased biomarkers of cytotoxicity and apoptosis. Further mechanistic and profiling studies revealed that ivermectin nonspecifically perturbs membrane bilayers at the same concentrations where it decreases the SARS-CoV-2 viral burden, resulting in nonspecific modulation of membrane-based targets such as G-protein coupled receptors and ion channels. These results suggest that a primary molecular mechanism for the in vitro antiviral activity of ivermectin may be nonspecific membrane perturbation, indicating that ivermectin is unlikely to be translatable into a safe and effective antiviral agent. These results and experimental workflow provide a useful paradigm for performing preclinical studies on (pandemic-related) drug repurposing candidates.
    Keywords covid19
    Language English
    Publishing date 2023-10-24
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2023.10.23.563088
    Database COVID19

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  9. Article: Concurrent isolation of hepatic stem cells and hepatocytes from the human liver

    Lee, Serene M. L / Bertinetti-Lapatki, Cristina / Schiergens, Tobias S / Jauch, Karl-Walter / Roth, Adrian B / Thasler, Wolfgang E

    In vitro cellular & developmental biology. 2020 Mar., v. 56, no. 3

    2020  

    Abstract: Hepatocytes differentiated from induced pluripotent stem cells or stem cells have the potential to be representative in vitro models of the human liver for research as well as early safety assessment programs. However, up until now, there has been no ... ...

    Abstract Hepatocytes differentiated from induced pluripotent stem cells or stem cells have the potential to be representative in vitro models of the human liver for research as well as early safety assessment programs. However, up until now, there has been no definitive proof that differentiated hepatocytes recapitulate the phenotype and functional characteristics of primary hepatocytes from the same individual. Thus, a method for the concurrent isolation of hepatocytes and hepatic stem cells is presented here to provide the cells necessary for the evaluation of the required benchmarking. The method presented here generated high-quality hepatocytes with a purity of 94 ± 1% and a high percentage viability of 79 ± 2%. Furthermore, the hepatic stem cells isolated were found to be actively proliferating and have a purity of 98 ± 1%. Thus, these isolated cells can be used as a powerful tool for the validation of differentiated hepatocyte in vitro models.
    Keywords functional properties ; hepatocytes ; humans ; induced pluripotent stem cells ; liver ; models ; phenotype ; safety assessment ; viability
    Language English
    Dates of publication 2020-03
    Size p. 253-260.
    Publishing place Springer US
    Document type Article
    ISSN 1071-2690
    DOI 10.1007/s11626-020-00433-w
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Transcriptomic Analysis of

    Roth, Myriam / Jaquet, Vincent / Lemeille, Sylvain / Bonetti, Eve-Julie / Cambet, Yves / François, Patrice / Krause, Karl-Heinz

    Antioxidants (Basel, Switzerland)

    2022  Volume 11, Issue 4

    Abstract: Hydrogen peroxide ( ... ...

    Abstract Hydrogen peroxide (H
    Language English
    Publishing date 2022-03-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox11040655
    Database MEDical Literature Analysis and Retrieval System OnLINE

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