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  1. Article ; Online: Introducing transparent peer review to

    Fisher, Laura / Cox, Russell J / Faulds, Karen

    RSC advances

    2023  Volume 13, Issue 32, Page(s) 21850–21851

    Abstract: ... RSC ... ...

    Abstract RSC Advances
    Language English
    Publishing date 2023-07-19
    Publishing country England
    Document type Editorial
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d3ra90060f
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Scalable slot-die coated flexible supercapacitors from upcycled PET face shields.

    Reddygunta, Kiran Kumar Reddy / Callander, Andrew / Šiller, Lidija / Faulds, Karen / Berlouis, Leonard / Ivaturi, Aruna

    RSC advances

    2024  Volume 14, Issue 18, Page(s) 12781–12795

    Abstract: Upcycling Covid19 plastic waste into valuable carbonaceous materials for energy storage applications is a sustainable and green approach to minimize the burden of waste plastic on the environment. Herein, we developed a facile single step activation ... ...

    Abstract Upcycling Covid19 plastic waste into valuable carbonaceous materials for energy storage applications is a sustainable and green approach to minimize the burden of waste plastic on the environment. Herein, we developed a facile single step activation technique for producing activated carbon consisting of spherical flower like carbon nanosheets and amorphous porous flakes from used PET [poly(ethylene terephthalate)] face shields for supercapacitor applications. The as-obtained activated carbon exhibited a high specific surface area of 1571 m
    Language English
    Publishing date 2024-04-19
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d2ra06809e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Spectral fingerprinting of cellular lipid droplets using stimulated Raman scattering microscopy and chemometric analysis.

    Rensonnet, Aurélie / Tipping, William J / Malherbe, Cedric / Faulds, Karen / Eppe, Gauthier / Graham, Duncan

    The Analyst

    2024  Volume 149, Issue 2, Page(s) 553–562

    Abstract: Hyperspectral stimulated Raman scattering (SRS) microscopy is a powerful method for direct visualisation and compositional analysis of cellular lipid droplets. Here we report the application of spectral phasor analysis as a convenient method for the ... ...

    Abstract Hyperspectral stimulated Raman scattering (SRS) microscopy is a powerful method for direct visualisation and compositional analysis of cellular lipid droplets. Here we report the application of spectral phasor analysis as a convenient method for the segmentation of lipid droplets using the hyperspectral SRS spectrum in the high wavenumber and fingerprint region of the spectrum. Spectral phasor analysis was shown to discriminate six fatty acids based on vibrational spectroscopic features in solution. The methodology was then applied to studying fatty acid metabolism and storage in a mammalian cancer cell model and during drug-induced steatosis in a hepatocellular carcinoma cell model. The accumulation of fatty acids into cellular lipid droplets was shown to vary as a function of the degree of unsaturation, whilst in a model of drug-induced steatosis, the detection of increased saturated fatty acid esters was observed. Taking advantage of the fingerprint and high wavenumber regions of the SRS spectrum has yielded a greater insight into lipid droplet composition in a cellular context. This approach will find application in the label-free profiling of intracellular lipids in complex disease models.
    MeSH term(s) Animals ; Lipid Droplets ; Chemometrics ; Nonlinear Optical Microscopy ; Fatty Acids ; Microscopy/methods ; Spectrum Analysis, Raman/methods ; Mammals
    Chemical Substances Fatty Acids
    Language English
    Publishing date 2024-01-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d3an01684f
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2423: Show issues Location:
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  4. Article ; Online: Label-Free Cytometric Evaluation of Mitosis via Stimulated Raman Scattering Microscopy and Spectral Phasor Analysis.

    Hislop, Ewan W / Tipping, William J / Faulds, Karen / Graham, Duncan

    Analytical chemistry

    2023  Volume 95, Issue 18, Page(s) 7244–7253

    Abstract: Hyperspectral stimulated Raman scattering (SRS) microscopy is a robust imaging tool for the analysis of biological systems. Here, we present a unique perspective, a label-free spatiotemporal map of mitosis, by integrating hyperspectral SRS microscopy ... ...

    Abstract Hyperspectral stimulated Raman scattering (SRS) microscopy is a robust imaging tool for the analysis of biological systems. Here, we present a unique perspective, a label-free spatiotemporal map of mitosis, by integrating hyperspectral SRS microscopy with advanced chemometrics to assess the intrinsic biomolecular properties of an essential process of mammalian life. The application of spectral phasor analysis to multiwavelength SRS images in the high-wavenumber (HWN) region of the Raman spectrum enabled the segmentation of subcellular organelles based on innate SRS spectra. Traditional imaging of DNA is primarily reliant on using fluorescent probes or stains which can affect the biophysical properties of the cell. Here, we demonstrate the label-free visualization of nuclear dynamics during mitosis coupled with an evaluation of its spectral profile in a rapid and reproducible manner. These results provide a snapshot of the cell division cycle and chemical variability between intracellular compartments in single-cell models, which is central to understanding the molecular foundations of these fundamental biological processes. The evaluation of HWN images by phasor analysis also facilitated the differentiation between cells in separate phases of the cell cycle based solely on their nuclear SRS spectral signal, which offers an interesting label-free approach in combination with flow cytometry. Therefore, this study demonstrates that SRS microscopy combined with spectral phasor analysis is a valuable method for detailed optical fingerprinting at the subcellular level.
    MeSH term(s) Animals ; Mitosis ; Nonlinear Optical Microscopy/methods ; Microscopy ; Cell Nucleus ; Spectrum Analysis, Raman/methods ; Mammals
    Language English
    Publishing date 2023-04-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.3c00212
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Analytical nanoscience.

    Algar, W Russ / Albrecht, Tim / Faulds, Karen / Zhu, Jun-Jie

    The Analyst

    2022  Volume 147, Issue 5, Page(s) 765–766

    Language English
    Publishing date 2022-02-28
    Publishing country England
    Document type Editorial
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d1an90110a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Label-Free Imaging of Lipid Droplets in Prostate Cells Using Stimulated Raman Scattering Microscopy and Multivariate Analysis.

    Hislop, Ewan W / Tipping, William J / Faulds, Karen / Graham, Duncan

    Analytical chemistry

    2022  Volume 94, Issue 25, Page(s) 8899–8908

    Abstract: Hyperspectral stimulated Raman scattering (SRS) microscopy is a powerful imaging modality for the analysis of biological systems. Here, we report the application ... ...

    Abstract Hyperspectral stimulated Raman scattering (SRS) microscopy is a powerful imaging modality for the analysis of biological systems. Here, we report the application of
    MeSH term(s) Humans ; Lipid Droplets/chemistry ; Lipids/analysis ; Male ; Multivariate Analysis ; Nonlinear Optical Microscopy/methods ; Prostate/chemistry ; Prostate/diagnostic imaging ; Prostatic Neoplasms/diagnostic imaging ; Spectrum Analysis, Raman/methods
    Chemical Substances Lipids
    Language English
    Publishing date 2022-06-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c00236
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Light-up split aptamers: binding thermodynamics and kinetics for sensing.

    Zhao, Yichen / Patel, Nikesh / Sun, Peihuan / Faulds, Karen / Graham, Duncan / Liu, Juewen

    The Analyst

    2023  Volume 148, Issue 22, Page(s) 5612–5618

    Abstract: Due to their programmable structures, many aptamers can be readily split into two halves while still retaining their target binding function. While split aptamers are prevalent in the biosensor field, fundamental studies of their binding are still ... ...

    Abstract Due to their programmable structures, many aptamers can be readily split into two halves while still retaining their target binding function. While split aptamers are prevalent in the biosensor field, fundamental studies of their binding are still lacking. In this work, we took advantage of the fluorescence enhancement property of a new aptamer named OTC5 that can bind to tetracycline antibiotics to compare various split aptamers with the full-length aptamer. The split aptamers were designed to have different stem lengths. Longer stem length aptamers showed similar dissociation constants (
    MeSH term(s) Gold/chemistry ; Aptamers, Nucleotide/chemistry ; Metal Nanoparticles/chemistry ; Thermodynamics
    Chemical Substances Gold (7440-57-5) ; Aptamers, Nucleotide
    Language English
    Publishing date 2023-11-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d3an01368e
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    Zs.A 2423: Show issues Location:
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  8. Article ; Online: Multiplex detection of the big five carbapenemase genes using solid-phase recombinase polymerase amplification.

    Johnson, Christopher L / Setterfield, Matthew A / Hassanain, Waleed A / Wipat, Anil / Pocock, Matthew / Faulds, Karen / Graham, Duncan / Keegan, Neil

    The Analyst

    2024  Volume 149, Issue 5, Page(s) 1527–1536

    Abstract: Five carbapenemase enzymes, coined the 'big five', have been identified as the biggest threat to worldwide antibiotic resistance based on their broad substrate affinity and global prevalence. Here we show the development of a molecular detection method ... ...

    Abstract Five carbapenemase enzymes, coined the 'big five', have been identified as the biggest threat to worldwide antibiotic resistance based on their broad substrate affinity and global prevalence. Here we show the development of a molecular detection method for the gene sequences from the five carbapenemases utilising the isothermal amplification method of recombinase polymerase amplification (RPA). We demonstrate the successful detection of each of the big five carbapenemase genes with femtomolar detection limits using a spatially separated multiplex amplification strategy. The approach uses tailed oligonucleotides for hybridisation, reducing the complexity and cost of the assay compared to classical RPA detection strategies. The reporter probe, horseradish peroxidase, generates the measureable output on a benchtop microplate reader, but more notably, our study leverages the power of a portable Raman spectrometer, enabling up to a 19-fold enhancement in the limit of detection. Significantly, the development approach employed a solid-phase RPA format, wherein the forward primers targeting each of the five carbapenemase genes are immobilised to a streptavidin-coated microplate. The adoption of this solid-phase methodology is pivotal for achieving a successful developmental pathway when employing this streamlined approach. The assay takes 2 hours until result, including a 40 minutes RPA amplification step at 37 °C. This is the first example of using solid-phase RPA for the detection of the big five and represents a milestone towards the developments of an automated point-of-care diagnostic for the big five using RPA.
    MeSH term(s) Recombinases/chemistry ; Nucleic Acid Amplification Techniques/methods ; Bacterial Proteins/genetics ; beta-Lactamases/genetics ; Sensitivity and Specificity
    Chemical Substances Recombinases ; carbapenemase (EC 3.5.2.6) ; Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2024-02-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d3an01747h
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Correction: Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications.

    Berry, Matthew E / Kearns, Hayleigh / Graham, Duncan / Faulds, Karen

    The Analyst

    2021  Volume 146, Issue 20, Page(s) 6335–6336

    Abstract: Correction for 'Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications' by Matthew E. ... ...

    Abstract Correction for 'Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications' by Matthew E. Berry
    Language English
    Publishing date 2021-10-11
    Publishing country England
    Document type Published Erratum
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d1an90082j
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Depth prediction of nanotags in tissue using surface enhanced spatially offset Raman scattering (SESORS).

    Berry, Matthew E / McCabe, Samantha M / Shand, Neil C / Graham, Duncan / Faulds, Karen

    Chemical communications (Cambridge, England)

    2022  Volume 58, Issue 11, Page(s) 1756–1759

    Abstract: A model for the prediction of the depth of two 'flavours' of surface enhanced Raman scattering (SERS) active nanotags embedded within porcine tissue is demonstrated using ratiometric analysis. Using a handheld spatially offset Raman (SORS) instrument, ... ...

    Abstract A model for the prediction of the depth of two 'flavours' of surface enhanced Raman scattering (SERS) active nanotags embedded within porcine tissue is demonstrated using ratiometric analysis. Using a handheld spatially offset Raman (SORS) instrument, SESORS signals could be detected from nanotags at depths down to 48 mm for the first time using a backscattering SORS geometry.
    Language English
    Publishing date 2022-02-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d1cc04455a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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