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Article: MEIS C termini harbor transcriptional activation domains that respond to cell signaling.

Huang, He / Rastegar, Mojgan / Bodner, Caroline / Goh, Siew-Lee / Rambaldi, Isabel / Featherstone, Mark

2005 Volume 280, Issue 11, Page(s) 10119–10127

Abstract: ... C termini. Fine mutation of the 56-residue MEIS1A C terminus revealed four discrete regions required ... implying a common mechanistic basis. C-terminal deletion of MEIS1 impaired transactivation ... a shared ability to form heteromeric complexes with PBX and HOX partners, the PREP1 C terminus does not ...

Abstract	MEIS proteins form heteromeric DNA-binding complexes with PBX monomers and PBX.HOX heterodimers. We have shown previously that transcriptional activation by PBX.HOX is augmented by either protein kinase A (PKA) or the histone deacetylase inhibitor trichostatin A (TSA). To examine the contribution of MEIS proteins to this response, we used the chromatin immunoprecipitation assay to show that MEIS1 in addition to PBX1, HOXA1, and HOXB1 was recruited to a known PBX.HOX target, the Hoxb1 autoregulatory element following Hoxb1 transcriptional activation in P19 cells. Subsequent to TSA treatment, MEIS1 recruitment lagged behind that of HOX and PBX partners. MEIS1A also enhanced the transcriptional activation of a reporter construct bearing the Hoxb1 autoregulatory element after treatment with TSA. The MEIS1 homeodomain and protein-protein interaction with PBX contributed to this activity. We further mapped TSA-responsive and CREB-binding protein-dependent PKA-responsive transactivation domains to the MEIS1A and MEIS1B C termini. Fine mutation of the 56-residue MEIS1A C terminus revealed four discrete regions required for transcriptional activation function. All of the mutations impairing the response to TSA likewise reduced activation by PKA, implying a common mechanistic basis. C-terminal deletion of MEIS1 impaired transactivation without disrupting DNA binding or complex formation with HOX and PBX. Despite sequence similarity to MEIS and a shared ability to form heteromeric complexes with PBX and HOX partners, the PREP1 C terminus does not respond to TSA or PKA. Thus, MEIS C termini possess transcriptional regulatory domains that respond to cell signaling and confer functional differences between MEIS and PREP proteins.
MeSH term(s)	Alanine/chemistry ; Animals ; Catalytic Domain ; Cell Line ; Cell Line, Tumor ; Chromatin Immunoprecipitation ; Cyclic AMP-Dependent Protein Kinases/chemistry ; Cyclic AMP-Dependent Protein Kinases/metabolism ; DNA/chemistry ; Dimerization ; Enzyme Inhibitors/pharmacology ; Gene Deletion ; Genes, Reporter ; Homeodomain Proteins/chemistry ; Homeodomain Proteins/metabolism ; Humans ; Hydroxamic Acids/pharmacology ; Luciferases/metabolism ; Mice ; Models, Biological ; Models, Genetic ; Mutation ; Myeloid Ecotropic Viral Integration Site 1 Protein ; Neoplasm Proteins/chemistry ; Neoplasm Proteins/metabolism ; Plasmids/metabolism ; Protein Binding ; Protein Biosynthesis ; Protein Structure, Tertiary ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Transcription, Genetic ; Transcriptional Activation
Chemical Substances	Enzyme Inhibitors ; HOXB1 homeodomain protein ; Homeodomain Proteins ; Hydroxamic Acids ; MEIS1 protein, human ; Meis1 protein, mouse ; Myeloid Ecotropic Viral Integration Site 1 Protein ; Neoplasm Proteins ; trichostatin A (3X2S926L3Z) ; DNA (9007-49-2) ; Luciferases (EC 1.13.12.-) ; Cyclic AMP-Dependent Protein Kinases (EC 2.7.11.11) ; Alanine (OF5P57N2ZX)
Language	English
Publishing date	2005-01-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.M413963200
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Article ; Online: A reply to: Young C, Beasley R. "The association between SpO2 values and mortality--interpret with caution".

Smith, Gary B / Prytherch, David R / Schmidt, Paul E / Featherstone, Peter I / Meredith, Paul

Resuscitation

2013 Volume 84, Issue 2, Page(s) e43

MeSH term(s)	Emergency Treatment ; Female ; Humans ; Male ; Oxygen/blood ; Oxygen Inhalation Therapy/standards
Chemical Substances	Oxygen (S88TT14065)
Language	English
Publishing date	2013-02
Publishing country	Ireland
Document type	Comment ; Letter
ZDB-ID	189901-6
ISSN	1873-1570 ; 0300-9572
ISSN (online)	1873-1570
ISSN	0300-9572
DOI	10.1016/j.resuscitation.2012.11.009
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Article ; Online: The effect of missing data on evolutionary analysis of sequence capture bycatch, with application to an agricultural pest.

Featherstone, Leo A / McGaughran, Angela

Molecular genetics and genomics : MGG

2024 Volume 299, Issue 1, Page(s) 11

Abstract: ... mitochondrial cytochrome c oxidase subunit I (COI) sequences across the species' global distribution. Using ...

Abstract	Sequence capture is a genomic technique that selectively enriches target sequences before high throughput next-generation sequencing, to generate specific sequences of interest. Off-target or 'bycatch' data are often discarded from capture experiments, but can be leveraged to address evolutionary questions under some circumstances. Here, we investigated the effects of missing data on a variety of evolutionary analyses using bycatch from an exon capture experiment on the global pest moth, Helicoverpa armigera. We added > 200 new samples from across Australia in the form of mitogenomes obtained as bycatch from targeted sequence capture, and combined these into an additional larger dataset to total > 1000 mitochondrial cytochrome c oxidase subunit I (COI) sequences across the species' global distribution. Using discriminant analysis of principal components and Bayesian coalescent analyses, we showed that mitogenomes assembled from bycatch with up to 75% missing data were able to return evolutionary inferences consistent with higher coverage datasets and the broader literature surrounding H. armigera. For example, low-coverage sequences broadly supported the delineation of two H. armigera subspecies and also provided new insights into the potential for geographic turnover among these subspecies. However, we also identified key effects of dataset coverage and composition on our results. Thus, low-coverage bycatch data can offer valuable information for population genetic and phylodynamic analyses, but caution is required to ensure the reduced information does not introduce confounding factors, such as sampling biases, that drive inference. We encourage more researchers to consider maximizing the potential of the targeted sequence approach by examining evolutionary questions with their off-target bycatch where possible-especially in cases where no previous mitochondrial data exists-but recommend stratifying data at different genome coverage thresholds to separate sampling effects from genuine genomic signals, and to understand their implications for evolutionary research.
MeSH term(s)	Bayes Theorem ; Agriculture ; Australia ; Biological Evolution ; Exons
Language	English
Publishing date	2024-02-21
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2044817-X
ISSN	1617-4623 ; 1617-4615
ISSN (online)	1617-4623
ISSN	1617-4615
DOI	10.1007/s00438-024-02097-7
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Article ; Online: Deaths.

Featherstone, C A

The Veterinary record

2017 Volume 180, Issue 13, Page(s) 332–333

Language	English
Publishing date	2017-04-01
Publishing country	England
Document type	Journal Article
ZDB-ID	390015-0
ISSN	2042-7670 ; 0042-4900
ISSN (online)	2042-7670
ISSN	0042-4900
DOI	10.1136/vr.j1560
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Article: Multidrug resistance in a urothelial cancer cell line after 3, 1-hour exposures to mitomycin C.

Speers, Alan G / Lwaleed, Bashir A / Featherstone, Jonathan M / Cooper, Alan J

The Journal of urology

2007 Volume 178, Issue 5, Page(s) 2171–2175

Abstract: ... Braunschweig, Germany) to mitomycin C, which is a common intravesical agent, would elicit multidrug resistance ... The highest mitomycin C concentrations permitting recovery in cultures and, therefore, available ... and exposed to high dose mitomycin C to assess the probability that the relevant concentrations ...

Abstract	Purpose: The development of multidrug resistance is a problem in chemotherapy for many tumors. In vitro models of multidrug resistance require adapted cell strains that are conventionally produced from parental lines by chronic low dose drug exposure. Because adjunctive intravesical chemotherapy for superficial bladder cancer uses short courses of high dose treatment, we investigated whether such exposure of the RT112 cell line (Catalogue No. ACC 418, Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany) to mitomycin C, which is a common intravesical agent, would elicit multidrug resistance. Materials and methods: Three 1-hour exposures to graded concentrations were done at 3-week intervals. The highest mitomycin C concentrations permitting recovery in cultures and, therefore, available for examination were 3.13 and 1.06 microg/ml. Cross-resistance to epirubicin in surviving cultures was visualized by confocal microscopy and quantified by MTT residual viable biomass assay. Spheroids were made by the agarose technique and exposed to high dose mitomycin C to assess the probability that the relevant concentrations might be found clinically in some cell layers of a superficial lesion. Results: Resistance was induced by 3 short drug exposures. The evidence for this was functional (MTT assay) and by intracellular localization. Toxicity to an alternative multidrug resistance class drug was lowered in surviving clones and nuclear exclusion of the drug was noted. Spheroid experiments showed sharp gradients of incorporated drug across the outermost layers of cells, suggesting that a proportion of cells in clinical superficial bladder cancer would be exposed to drug at concentrations that generated the resistant clones in these experiments. Conclusions: We report multidrug resistance induction using 2 independent methodologies. The results have implications for the development of experimental models and the likelihood of resistance resulting from clinical regimens. Brief exposure can elicit detectable resistance. It is arguable that selective rather than instructive mechanisms are involved, and the levels of drug required are likely to exist in a superficial transitional cell carcinoma frond exposed at its surface to high drug concentrations.
MeSH term(s)	Antibiotics, Antineoplastic/administration & dosage ; Antibiotics, Antineoplastic/pharmacokinetics ; Carcinoma, Transitional Cell/drug therapy ; Carcinoma, Transitional Cell/metabolism ; Carcinoma, Transitional Cell/pathology ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Drug Resistance, Neoplasm ; Epirubicin/administration & dosage ; Epirubicin/pharmacokinetics ; Follow-Up Studies ; Humans ; Intracellular Fluid/metabolism ; Mitomycin/administration & dosage ; Mitomycin/pharmacokinetics ; Phenotype ; Spheroids, Cellular/metabolism ; Spheroids, Cellular/pathology ; Time Factors ; Urinary Bladder Neoplasms/drug therapy ; Urinary Bladder Neoplasms/metabolism ; Urinary Bladder Neoplasms/pathology
Chemical Substances	Antibiotics, Antineoplastic ; Epirubicin (3Z8479ZZ5X) ; Mitomycin (50SG953SK6)
Language	English
Publishing date	2007-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	3176-8
ISSN	1527-3792 ; 0022-5347
ISSN (online)	1527-3792
ISSN	0022-5347
DOI	10.1016/j.juro.2007.06.047
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Article ; Online: Lichen planus follicularis tumidus of the vulva.

Termin, Denali E / Welch, Kathryn C / Haefner, Hope K / Margesson, Lynette J / Wang, Maxwell D / Saunders, Natalie A / Parker-Featherstone, Ebony C

American journal of obstetrics and gynecology

2024

Language	English
Publishing date	2024-03-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	80016-8
ISSN	1097-6868 ; 0002-9378
ISSN (online)	1097-6868
ISSN	0002-9378
DOI	10.1016/j.ajog.2024.03.004
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Article ; Online: A zebrafish hox gene acts before gastrulation to specify the hemangioblast.

Zhang, Changqing / Featherstone, Mark

Genesis (New York, N.Y. : 2000)

2020 Volume 58, Issue 6, Page(s) e23363

Abstract: Hox genes encode transcription factors that have been implicated in embryonic, adult and disease processes. The earliest developmental program known to be directed by Hox genes is the timing of ingression of presumptive axial mesoderm during gastrulation. ...

Abstract	Hox genes encode transcription factors that have been implicated in embryonic, adult and disease processes. The earliest developmental program known to be directed by Hox genes is the timing of ingression of presumptive axial mesoderm during gastrulation. We previously used morpholino (MO)-based knockdown to implicate the zebrafish hoxd4a gene in the specification of the hemangioblast, an event occurring at pre-gastrulation stages, well before the earliest known Hox gene function. The precise time at which hoxd4a function is required for this specification is not defined. We therefore fused the hoxd4a coding region to the human estrogen receptor (hER
MeSH term(s)	Animals ; Cell Differentiation ; Gastrulation ; Gene Expression Regulation, Developmental ; Genes, Homeobox ; Hemangioblasts/cytology ; Hemangioblasts/metabolism ; Humans ; Receptors, Estrogen/genetics ; Receptors, Estrogen/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Zebrafish
Chemical Substances	Receptors, Estrogen ; Recombinant Proteins
Language	English
Publishing date	2020-04-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2004544-X
ISSN	1526-968X ; 1526-954X
ISSN (online)	1526-968X
ISSN	1526-954X
DOI	10.1002/dvg.23363
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Article ; Online: SERINC5 Inhibits HIV-1 Infectivity by Altering the Conformation of gp120 on HIV-1 Particles.

Featherstone, Austin / Aiken, Christopher

Journal of virology

2020 Volume 94, Issue 20

Abstract: SERINC5 is a 10-transmembrane-domain cellular protein that is incorporated into budding HIV-1 particles and reduces HIV-1 infectivity by inhibiting virus-cell fusion. HIV-1 susceptibility to SERINC5 is determined by sequences in the viral Env ... ...

Abstract	SERINC5 is a 10-transmembrane-domain cellular protein that is incorporated into budding HIV-1 particles and reduces HIV-1 infectivity by inhibiting virus-cell fusion. HIV-1 susceptibility to SERINC5 is determined by sequences in the viral Env glycoprotein gp120, and the antiviral effect of SERINC5 is counteracted by the viral accessory protein Nef. While the precise mechanism by which SERINC5 inhibits HIV-1 infectivity is unclear, previous studies have suggested that SERINC5 affects Env conformation. To define the effects of SERINC5 on Env conformation, we quantified the binding of HIV-1 particles to immobilized Env-specific monoclonal antibodies. We observed that SERINC5 reduced the binding of HIV-1 particles bearing a SERINC5-susceptible Env to antibodies that recognize the V3 loop, a soluble CD4 (sCD4)-induced epitope, and an N-linked glycan. In contrast, SERINC5 did not alter the capture of HIV-1 particles bearing the SERINC5-resistant Env protein. Moreover, the effect of SERINC5 on antibody-dependent virus capture was abrogated by Nef expression. Our results indicate that SERINC5 inhibits HIV-1 infectivity by altering the conformation of gp120 on virions and/or physical masking of specific HIV-1 Env epitopes.
MeSH term(s)	CD4 Antigens/genetics ; CD4 Antigens/metabolism ; HEK293 Cells ; HIV Envelope Protein gp120/genetics ; HIV Envelope Protein gp120/metabolism ; HIV Infections/genetics ; HIV Infections/metabolism ; HIV-1/genetics ; HIV-1/metabolism ; Humans ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Protein Structure, Secondary
Chemical Substances	CD4 Antigens ; HIV Envelope Protein gp120 ; Membrane Proteins ; SERINC5 protein, human ; gp120 protein, Human immunodeficiency virus 1
Language	English
Publishing date	2020-09-29
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.00594-20
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Article ; Online: The Use of a Constructivist Grounded Theory Method - A Good Fit for Social Work Research

Annaley Clarke / Karen Healy / Deborah Lynch / Gerald Featherstone

International Journal of Qualitative Methods, Vol

2023 Volume 22

Abstract: This paper explores the application of Constructivist Grounded Theory (C-GT) methodology ... for social work research. First, it argues that C-GT methodology is well aligned with social work as the two value ... which also focus on the importance of professional integrity. The paper then provides an example of a C-GT ...

Abstract	This paper explores the application of Constructivist Grounded Theory (C-GT) methodology for social work research. First, it argues that C-GT methodology is well aligned with social work as the two value the individual in the context of their environmental influences. Both also prioritise the importance of respecting and valuing the participant or individual, seeing their perspective as unique and significant. Finally, this research methodology’s systematic yet flexible guidelines align with social work ethics, which also focus on the importance of professional integrity. The paper then provides an example of a C-GT PhD study in relation to an area of social work practice, that is, stability in statutory kinship care in Queensland, Australia. The paper details the specific recruitment and sampling of participants, data collection and coding analysis examples from the PhD study. The examples evidence the relevance of C-GT methodology and its application for research in the area of social work practice, specifically statutory kinship care.
Keywords	Social sciences (General) ; H1-99
Subject code	300
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	SAGE Publishing
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Opioid toxicity after oxycodone/naloxone to oxycodone conversion: case series.

Doherty, Mairead / Featherstone, Hannah Joan / McAleer, Clare / Webb, Chloe / O'Reilly, Maeve / Twomey, Marie / McQuillan, Regina

BMJ supportive & palliative care

2024

Abstract: Combination preparations of oxycodone/naloxone are marketed to aid in the management of opioid induced bowel dysfunction, with caution advised in prescribing in cases of liver dysfunction.This case series demonstrates four cases of patients with normal ... ...

Abstract	Combination preparations of oxycodone/naloxone are marketed to aid in the management of opioid induced bowel dysfunction, with caution advised in prescribing in cases of liver dysfunction.This case series demonstrates four cases of patients with normal liver function tests who developed significant opioid toxicity on conversion from combination oxycodone/naloxone to oxycodone at equivalent doses, necessitating significant dose reduction.In each case, a cause for intra-hepatic shunting such as cirrhosis, porto-systemic collaterals or thrombosis were identified, highlighting these as cautionary features when prescribing combination preparations of oxycodone/naloxone and the possible need for dose reduction if converting to oxycodone.
Language	English
Publishing date	2024-02-26
Publishing country	England
Document type	Journal Article
ISSN	2045-4368
ISSN (online)	2045-4368
DOI	10.1136/spcare-2024-004796
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