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  1. Article ; Online: Mucoadhesive phenylboronic acid-grafted carboxymethyl cellulose hydrogels containing glutathione for treatment of corneal epithelial cells exposed to benzalkonium chloride.

    Cheng, Yung-Hsin / Huang, Hao-Pai / Chen, Hsin-Ho

    Colloids and surfaces. B, Biointerfaces

    2024  Volume 238, Page(s) 113884

    Abstract: Benzalkonium chloride (BAK) is the most commonly-used preservative in topical ophthalmic medications that may cause ocular surface inflammation associated with oxidative stress and dry eye syndrome. Glutathione (GSH) is an antioxidant in human tears and ... ...

    Abstract Benzalkonium chloride (BAK) is the most commonly-used preservative in topical ophthalmic medications that may cause ocular surface inflammation associated with oxidative stress and dry eye syndrome. Glutathione (GSH) is an antioxidant in human tears and able to decrease the proinflammatory cytokine release from cells and reactive oxygen species (ROS) formation. Carboxymethyl cellulose (CMC), a hydrophilic polymer, is one of most commonly used artificial tears and can promote the corneal epithelial cell adhesion, migration and re-epithelialization. However, most of commercial artificial tears provide only temporary relief of irritation symptoms and show the short-term treatment effects. In the study, 3-aminophenylboronic acid was grafted to CMC for increase of mucoadhesive properties that might increase the precorneal retention time and maintain the effective therapeutic concentration on the ocular surface. CMC was modified with different degree of substitution (DS) and characterized by Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Phenylboronic acid (PBA)-grafted CMC hydrogels have interconnected porous structure and shear thinning behavior. Modification of CMC with high DS (H-PBA-CMC) shows the strong bioadhesive force. The optimal concentration of GSH to treat corneal epithelial cells (CECs) was evaluated by cell viability assay. H-PBA-CMC hydrogels could sustained release GSH and decrease the ROS level. H-PBA-CMC hydrogels containing GSH shows the therapeutic effects in BAK-damaged CECs via improvement of inflammation, apoptosis and cell viability. After topical administration of developed hydrogels, there was no ocular irritation in rabbits. These results suggested that PBA-grafted CMC hydrogels containing GSH might have potential applications for treatment of dry eye disease.
    Language English
    Publishing date 2024-03-29
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1500523-9
    ISSN 1873-4367 ; 0927-7765
    ISSN (online) 1873-4367
    ISSN 0927-7765
    DOI 10.1016/j.colsurfb.2024.113884
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Effects of Exercise Training on Cognitive Function in Individuals With Heart Failure: A Meta-Analysis.

    Peng, Jing-Ya / Chen, Yung-Hsin / Yen, Ju-Hsin / Huang, Wei-Ming / Chen, Chiao-Nan

    Physical therapy

    2023  Volume 103, Issue 6

    Abstract: Objective: Cognitive function is critical for disease self-management; however, it is poorer in individuals with heart failure (HF) than in age-matched people who are healthy. Aging and disease progression collectively threaten the cognitive function of ...

    Abstract Objective: Cognitive function is critical for disease self-management; however, it is poorer in individuals with heart failure (HF) than in age-matched people who are healthy. Aging and disease progression collectively threaten the cognitive function of individuals with HF. Exercise has been shown to improve the mobility and mortality risk factors of this population, but the effects of exercise on the cognitive function of individuals with HF are unclear. This meta-analysis aimed to examine these potential effects.
    Methods: A systematic literature search was conducted in PubMed, MEDLINE, CINAHL, Cochrane Library, Physiotherapy Evidence Database, and ClinicalKey on literature published until January 2022. Studies examining the effects of exercise training on cognitive function in individuals with HF were included. Characteristics of participants and details of interventions were extracted. Effects of exercise training on global cognitive function, attention, and executive function were analyzed using the Comprehensive Meta-Analysis software.
    Results: Six studies were included. Individuals with chronic HF were examined in most studies. The average ejection fraction of participants was 23 to 46%. Aerobic exercise was used in most studies. All included studies had exercise frequency of 2 to 3 times per week and a duration of 30 to 60 minutes per session for 12 to 18 weeks. Compared with the control group, exercise training benefited the global cognitive function of individuals with HF and cognitive impairments (standardized mean difference = 0.44; 95% CI = 0.01-0.87). The attention of individuals with HF improved after exercise training compared with that before the intervention.
    Conclusion: Exercise may improve cognitive function in individuals with HF and cognitive impairments. However, due to large heterogeneity in the study design, more studies are needed to support clinical application.
    Impact: These findings should raise clinicians' awareness about the role of exercise on the cognitive function of individuals with HF, in addition to benefits in physical domains.
    MeSH term(s) Humans ; Quality of Life ; Exercise ; Heart Failure/therapy ; Cognition ; Health Status
    Language English
    Publishing date 2023-06-06
    Publishing country United States
    Document type Meta-Analysis ; Journal Article
    ZDB-ID 415886-6
    ISSN 1538-6724 ; 0031-9023
    ISSN (online) 1538-6724
    ISSN 0031-9023
    DOI 10.1093/ptj/pzad027
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Synthesis of a Multifunctional Glyco-Block Copolymer through Reversible Addition-Fragmentation Chain Transfer Polymerization and Click Chemistry for Enzyme and Drug Loading into MDA-MB-231 Cells.

    Wu, Tzu-Chien / Lai, Chiao-Ling / Sivakumar, Govindan / Huang, Yung-Hsin / Lai, Chian-Hui

    ACS applied materials & interfaces

    2023  Volume 15, Issue 51, Page(s) 59746–59759

    Abstract: Reversible addition-fragmentation chain transfer polymerization has been used in various applications such as preparing nanoparticles, stimulus-responsive polymers, and hydrogels. In this study, the combination of this polymerization method and Cu(I)- ... ...

    Abstract Reversible addition-fragmentation chain transfer polymerization has been used in various applications such as preparing nanoparticles, stimulus-responsive polymers, and hydrogels. In this study, the combination of this polymerization method and Cu(I)-catalyzed azide-alkyne cycloaddition click chemistry was used to prepare the multifunctional glyco-diblock copolymer
    MeSH term(s) Polymerization ; MDA-MB-231 Cells ; Glucose Oxidase ; Click Chemistry ; Azides ; Hydrogen Peroxide ; Drug Carriers ; Polymers/chemistry ; Doxorubicin/pharmacology ; Doxorubicin/chemistry ; Prodrugs/chemistry ; Nanoparticles/chemistry ; Alkynes ; Glucose
    Chemical Substances Glucose Oxidase (EC 1.1.3.4) ; Azides ; Hydrogen Peroxide (BBX060AN9V) ; Drug Carriers ; Polymers ; Doxorubicin (80168379AG) ; Prodrugs ; Alkynes ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2023-12-18
    Publishing country United States
    Document type Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.3c12184
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Human amniotic fluid stem cell therapy can help regain bladder function in type 2 diabetic rats.

    Liang, Ching-Chung / Shaw, Steven W / Huang, Yung-Hsin / Lee, Tsong-Hai

    World journal of stem cells

    2022  Volume 14, Issue 5, Page(s) 330–346

    Abstract: Background: Diabetes mellitus (DM) is a serious and growing global health burden. It is estimated that 80% of diabetic patients have micturition problems such as poor emptying, urinary incontinence, urgency, and urgency incontinence. Patients with ... ...

    Abstract Background: Diabetes mellitus (DM) is a serious and growing global health burden. It is estimated that 80% of diabetic patients have micturition problems such as poor emptying, urinary incontinence, urgency, and urgency incontinence. Patients with diabetic bladder dysfunction are often resistant to currently available therapies. It is necessary to develop new and effective treatment methods.
    Aim: To examine the therapeutic effect of human amniotic fluid stem cells (hAFSCs) therapy on bladder dysfunction in a type 2 diabetic rat model.
    Methods: Sixty female Sprague-Dawley rats were divided into five groups: Group 1, normal-diet control (control); group 2, high-fat diet (HFD); group 3, HFD plus streptozotocin-induced DM (DM); group 4, DM plus insulin treatment (DM + insulin); group 5, DM plus hAFSCs injection
    Results: Compared with DM rats, insulin but not hAFSCs treatment could reduce the bladder weight and improve the voided volume, intercontraction interval, bladder capacity, and residual volume (
    Conclusion: Insulin but not hAFSCs therapy can recover the bladder dysfunction caused by DM; however, hAFSCs and insulin therapy can help to regain bladder function to near the levels of control.
    Language English
    Publishing date 2022-06-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2583482-4
    ISSN 1948-0210
    ISSN 1948-0210
    DOI 10.4252/wjsc.v14.i5.330
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Urinary beta 3-adrenoceptor as a diagnostic biomarker for overactive bladder in women.

    Liang, Ching-Chung / Hsieh, Wu-Chiao / Lo, Tsia-Shu / Huang, Ting-Xuan / Chou, Yi-Chun / Huang, Jing-Yi / Huang, Yung-Hsin

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 19368

    Abstract: This study was to investigate urinary beta 3-adrenoceptor concentration as a biomarker for overactive bladder (OAB) and predictor of treatment outcomes in women receiving the beta 3-adrenoceptor agonist mirabegron. The study comprised 50 women identified ...

    Abstract This study was to investigate urinary beta 3-adrenoceptor concentration as a biomarker for overactive bladder (OAB) and predictor of treatment outcomes in women receiving the beta 3-adrenoceptor agonist mirabegron. The study comprised 50 women identified with OAB and 35 women considered as healthy controls. All women with OAB received daily dosage of 50 mg of mirabegron for 12 weeks. Bladder diaries, OAB-related questionnaires, and global response assessment scale (GRAS) data were collected. Urinary beta 3-adrenoceptor concentration was measured through enzyme-linked immunosorbent assay. All OAB-related questionnaires and GRAS indicated improved posttreatment urinary health. After mirabegron treatment, the frequency of micturition and urgency episodes decreased, but the urinary beta 3-adrenoceptor/creatinine (Cr) ratio increased. The urinary beta 3-adrenoceptor/creatinine ratio was identified as a sensitive biomarker for OAB with a confidence interval of 0.656 to 0.856 (p < 0.001). A negative correlation (- 0.431, p = 0.040) between this biomarker and health-related quality of life (HRQL) scores. The Beta 3-adrenoceptor/Cr levels increased significantly in the treatment-responsive group, while they remained unchanged in the unsatisfactory outcome group. This study shows that 12 weeks of mirabegron treatment improves OAB symptoms and HRQL. Furthermore, urinary beta 3-adrenoceptor concentration may be a diagnostic biomarker for OAB.
    MeSH term(s) Female ; Humans ; Creatinine ; Quality of Life ; Urinary Bladder, Overactive/diagnosis ; Urinary Bladder, Overactive/drug therapy ; Urinary Tract
    Chemical Substances Creatinine (AYI8EX34EU) ; mirabegron (MVR3JL3B2V) ; ADRB3 protein, human
    Language English
    Publishing date 2023-11-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-46786-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Human amniotic fluid stem cells can alleviate detrusor dysfunction caused by bladder outlet obstruction in rats.

    Liang, Ching-Chung / Huang, Wen-Chu / Shaw, Steven W / Huang, Yung-Hsin / Lee, Tsong-Hai

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 6679

    Abstract: The present study examined whether bladder detrusor dysfunction due to partial bladder outlet obstruction (pBOO) could be improved after the treatment of human amniotic fluid stem cells (hAFSCs). 72 female rats were grouped into sham operation, pBOO, and ...

    Abstract The present study examined whether bladder detrusor dysfunction due to partial bladder outlet obstruction (pBOO) could be improved after the treatment of human amniotic fluid stem cells (hAFSCs). 72 female rats were grouped into sham operation, pBOO, and pBOO with hAFSCs treatment (pBOO + hAFSCs) for in vitro and in vivo studies. Bladder weight, bladder wall thickness, the ratio of collagen to smooth muscle and the levels of positive CD11b/c and HIS48 cells was significantly increased after pBOO but improved after hAFSCs treatment. Cystometries showed impaired bladder function after pBOO. Protein and mRNA levels of hypoxia inducible factor-1α, CCL2, interleukin-1β, transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), α-smooth muscle actin, collagen I and collagen III were increased at 2 and/or 6 weeks, but proteins and mRNA expressions of protein gene product 9.5 were decreased at 2 and 6 weeks after pBOO. These abnormalities were improved after hAFSCs treatment. The expressions of TGF-β1 and CTGF in cultured detrusor cells of pBOO rats were increased but were improved after hAFSCs treatment. The present results showed hAFSCs treatment could improve bladder detrusor dysfunction in pBOO rats, which may be related to the reduction of inflammatory and pro-fibrotic markers in detrusor muscle cells.
    MeSH term(s) Amniotic Fluid/metabolism ; Animals ; Disease Models, Animal ; Female ; Humans ; Male ; RNA, Messenger/genetics ; Rats ; Stem Cells/metabolism ; Transforming Growth Factor beta1 ; Urinary Bladder Neck Obstruction/complications ; Urinary Bladder Neck Obstruction/genetics ; Urinary Bladder Neck Obstruction/therapy ; Urologic Diseases
    Chemical Substances RNA, Messenger ; Transforming Growth Factor beta1
    Language English
    Publishing date 2022-04-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-10640-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Human amniotic fluid stem cells can improve cerebral vascular remodelling and neurological function after focal cerebral ischaemia in diabetic rats.

    Liang, Ching-Chung / Shaw, Steven W / Huang, Yung-Hsin / Lee, Tsong-Hai

    Journal of cellular and molecular medicine

    2021  Volume 25, Issue 21, Page(s) 10185–10196

    Abstract: Diabetes causes vascular injury and carries a high risk of ischaemic stroke. Human amniotic fluid stem cells (hAFSCs) can enhance cerebral vascular remodelling and have the potential to improve neurological function after stroke in diabetic rats. Five ... ...

    Abstract Diabetes causes vascular injury and carries a high risk of ischaemic stroke. Human amniotic fluid stem cells (hAFSCs) can enhance cerebral vascular remodelling and have the potential to improve neurological function after stroke in diabetic rats. Five groups of female rats were examined: (1) normal control, (2) type 1 diabetic (T1DM) rats induced by streptozotocin injection (DM), (3) non-DM rats receiving 60-minute middle cerebral artery occlusion (MCAO), (4) T1DM rats receiving 60-minute MCAO (DM + MCAO) and (5) T1DM rats receiving 60-minute MCAO and injection with 5 × 10
    MeSH term(s) Amniotic Fluid/cytology ; Animals ; Biomarkers ; Blood Glucose ; Blood-Brain Barrier/metabolism ; Brain Ischemia/diagnosis ; Brain Ischemia/etiology ; Brain Ischemia/metabolism ; Brain Ischemia/therapy ; Diabetes Mellitus, Experimental ; Disease Models, Animal ; Disease Susceptibility ; Female ; Gene Expression Regulation ; Humans ; Immunohistochemistry ; Magnetic Resonance Imaging ; Matrix Metalloproteinase 9/metabolism ; Rats ; Stem Cell Transplantation ; Stem Cells/cytology ; Stem Cells/metabolism ; Vascular Remodeling
    Chemical Substances Biomarkers ; Blood Glucose ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Mmp9 protein, rat (EC 3.4.24.35)
    Language English
    Publishing date 2021-10-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2074559-X
    ISSN 1582-4934 ; 1582-4934 ; 1582-1838
    ISSN (online) 1582-4934
    ISSN 1582-4934 ; 1582-1838
    DOI 10.1111/jcmm.16956
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Robotic-Assisted Thoracoscopic (RATS) Enucleation of Esophageal Mesenchymal Tumors and Foregut Cysts.

    Chen, Yung-Hsin / Chen, Ke-Cheng / Huang, Pei-Ming / Kuo, Shuenn-Wen / Lin, Mong-Wei / Lee, Jang-Ming

    Journal of clinical medicine

    2022  Volume 11, Issue 21

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2022-10-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm11216471
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Effectiveness and Safety of Lacosamide, A Third-generation Anti-seizure Medication, for Poststroke Seizure and Epilepsy: A Literature Review.

    Chen, Yu-Shiue / Lai, Ming-Chi / Chen, Tsang-Shan / Tseng, Yung-Hsin / Li, Ya Jhen / Huang, Chin-Wei

    Current neuropharmacology

    2023  Volume 21, Issue 10, Page(s) 2126–2133

    Abstract: Advances in stroke treatment have resulted in a dramatic reduction in stroke mortality. Nevertheless, poststroke seizures and epilepsy are issues of clinical importance affecting survivors. Additionally, stroke is the most common cause of epilepsy in ... ...

    Abstract Advances in stroke treatment have resulted in a dramatic reduction in stroke mortality. Nevertheless, poststroke seizures and epilepsy are issues of clinical importance affecting survivors. Additionally, stroke is the most common cause of epilepsy in older adults. Although numerous antiseizure medications exist, studies are needed to provide robust evidence of the efficacy and tolerability of these medicines for treating poststroke seizures and epilepsy. Crucially, the newer generations of antiseizure medications require testing. Lacosamide, a third-generation antiseizure medication approved for treating localization-related epilepsy, has a novel mechanism of selectively enhancing the slow inactivation of sodium channels. This literature review evaluated whether lacosamide is effective and safe for the treatment of poststroke seizures and epilepsy. This review critically analyzed studies published in major academic databases (Pubmed, Embase, and Cochrane Library) from inception through June 2022 regarding the interaction of lacosamide with poststroke seizures and epilepsy. We included clinical prospective, retrospective, and case studies on patients with poststroke seizure and epilepsy, lacosamide as a treatment for seizures, neuroprotection in animal models of seizures, and the safety of lacosamide when coadministering anticoagulants. Clinical studies revealed lacosamide to be an effective antiseizure medication with high efficacy and tolerability in patients with poststroke seizures and epilepsy. In animal models, lacosamide proved effective at seizure reduction and neuroprotection. Pharmacokinetic studies demonstrated the safety of lacosamide when coadministering conventional and new anticoagulants. The literature suggests that Lacosamide is a promising candidate antiseizure medication for patients with poststroke seizures and epilepsy.
    MeSH term(s) Animals ; Lacosamide/therapeutic use ; Anticonvulsants/therapeutic use ; Retrospective Studies ; Prospective Studies ; Treatment Outcome ; Epilepsy/drug therapy ; Epilepsy/etiology ; Stroke/complications ; Stroke/drug therapy ; Anticoagulants/therapeutic use
    Chemical Substances Lacosamide (563KS2PQY5) ; Anticonvulsants ; Anticoagulants
    Language English
    Publishing date 2023-06-21
    Publishing country United Arab Emirates
    Document type Review ; Journal Article
    ZDB-ID 2192352-8
    ISSN 1875-6190 ; 1570-159X
    ISSN (online) 1875-6190
    ISSN 1570-159X
    DOI 10.2174/1570159X21666230616114255
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Association Between Risk of Clostridium difficile Infection and Duration of Proton Pump Inhibitor or H2-Receptor Antagonist Use in Hospitalized Patients.

    Huang, Chien-Huei / Tseng, Yung-Hsin / Tsai, Wen-Shan / Su, Chien-Chou / Cheng, Ching-Lan / Kao Yang, Yea-Huei / Chang, Yu-Ching / Liu, Yi-Hsuan

    Infectious diseases and therapy

    2024  Volume 13, Issue 2, Page(s) 373–383

    Abstract: Introduction: Limited studies have evaluated the association between Clostridium difficile infection (CDI) and the duration of proton pump inhibitor (PPI) or histamine H2-receptor blocker (H2RA) use and provided a cutoff duration for PPI or H2RA use to ... ...

    Abstract Introduction: Limited studies have evaluated the association between Clostridium difficile infection (CDI) and the duration of proton pump inhibitor (PPI) or histamine H2-receptor blocker (H2RA) use and provided a cutoff duration for PPI or H2RA use to mitigate a substantially increased risk of CDI. We aimed to evaluate these associations in hospitalized patients using a nationwide insurance claims database.
    Methods: We conducted a nested case-control study to identify cases with a first ever record of CDI in a study cohort undergoing PPI or H2RA therapy from the National Health Insurance Database from 2012 to 2018. Each case was matched with one control by age, sex, and calendar year. We used conditional logistic regression to estimate the sensitivity, specificity, and area under the receiver operating characteristic curve (AUC ROC). Youden's J statistic was used to identify the optimal cutoff duration in days for PPI or H2RA use.
    Results: In the main analysis, the AUC ROC was 0.64 (95% CI 0.63-0.66) and optimal cutoff duration was 15 days for PPI users. The AUC ROC was 0.63 (95% CI 0.62-0.64) and optimal cutoff duration was 16 days for H2RA users. In the sensitivity analyses, the results were similar to those of the main analysis, and the optimal cutoff duration was in the range of 14-15 days.
    Conclusions: The optimal cutoff duration for PPI and H2RA use was about 2 weeks. It is necessary to be cautious regarding the risk of CDI in patients taking PPIs or H2RAs for longer than 2 weeks.
    Language English
    Publishing date 2024-01-30
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2701611-0
    ISSN 2193-6382 ; 2193-8229
    ISSN (online) 2193-6382
    ISSN 2193-8229
    DOI 10.1007/s40121-024-00922-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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