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Article ; Online: Evaluation of the energy performance and cost-benefit of innovative technologies in butcher’s shops

Lippens Jeroen / Lokere Saar / Barbary Wout / Breesch Hilde

E3S Web of Conferences, Vol 246, p

2021 Volume 05003

Abstract: ... energy efficiency of innovative technologies. This paper is focussing on butcher’s shops. A reference model is made ...

Abstract	The CO2 emissions and energy use of SMEs in the tertiary sector (e.g. small food and non-food shops, restaurants, offices, pubs, etc.) are high and there are few initiatives to reduce because this target group is difficult to reach due to small scale and diversity. The Flemish-Dutch TERTS project wants (1) to make the sector aware of the potential of and (2) to demonstrate energy transition and energy efficiency of innovative technologies. This paper is focussing on butcher’s shops. A reference model is made based on data of 90 existing shops in Flanders (Belgium). The energy use of the building and systems is calculated according to DIN V 15 899. The cost-benefit of various measures is calculated and compared. Results show that the main energy consumers of a butcher shop are cooling, lighting and domestic hot water, whereas heating only has a rather small contribution. There are several cooling needs: product-cooling (in walk-in freezers, walk-in coolers and the cooling counter) and cooling of the workshop. The combination of the following measures is concluded to be the most favourable and leads to a reduction in final energy consumption of 60 %: a reflective coating on the flat roof and extra roof insulation, relighting with LED, air-to-water heat pump for the generation of domestic hot water and PV panels as local energy generation.
Keywords	Environmental sciences ; GE1-350
Subject code	690
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Can you keep up? : The challenges for research institutes and core facilities in scouting and adopting new technologies.

Van Minnebruggen, Geert / Lippens, Saskia

EMBO reports

2024 Volume 25, Issue 4, Page(s) 1704–1707

MeSH term(s)	Academies and Institutes
Language	English
Publishing date	2024-02-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2020896-0
ISSN	1469-3178 ; 1469-221X
ISSN (online)	1469-3178
ISSN	1469-221X
DOI	10.1038/s44319-024-00078-w
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Article ; Online: Future proofing core facilities with a seven-pillar model.

Tranfield, Erin M / Lippens, Saskia

Journal of microscopy

2024

Abstract: Centralised core facilities have evolved into vital components of life science research, transitioning from a primary focus on centralising equipment to ensuring access to technology experts across all facets of an experimental workflow. Herein, we put ... ...

Abstract	Centralised core facilities have evolved into vital components of life science research, transitioning from a primary focus on centralising equipment to ensuring access to technology experts across all facets of an experimental workflow. Herein, we put forward a seven-pillar model to define what a core facility needs to meet its overarching goal of facilitating research. The seven equally weighted pillars are Technology, Core Facility Team, Training, Career Tracks, Technical Support, Community and Transparency. These seven pillars stand on a solid foundation of cultural, operational and framework policies including the elements of transparent and stable funding strategies, modern human resources support, progressive facility leadership and management as well as clear institute strategies and policies. This foundation, among other things, ensures a tight alignment of the core facilities to the vision and mission of the institute. To future-proof core facilities, it is crucial to foster all seven of these pillars, particularly focusing on newly identified pillars such as career tracks, thus enabling core facilities to continue supporting research and catalysing scientific advancement. Lay abstract: In research, there is a growing trend to bring advanced, high-performance equipment together into a centralised location. This is done to streamline how the equipment purchase is financed, how the equipment is maintained, and to enable an easier approach for research scientists to access these tools in a location that is supported by a team of technology experts who can help scientists use the equipment. These centralised equipment centres are called Core Facilities. The core facility model is relatively new in science and it requires an adapted approach to how core facilities are built and managed. In this paper, we put forward a seven-pillar model of the important supporting elements of core facilities. These supporting elements are: Technology (the instruments themselves), Core Facility Team (the technology experts who operate the instruments), Training (of the staff and research community), Career Tracks (for the core facility staff), Technical Support (the process of providing help to apply the technology to a scientific question), Community (of research scientist, technology experts and developers) and Transparency (of how the core facility works and the costs associated with using the service). These pillars stand on the bigger foundation of clear policies, guidelines, and leadership approaches at the institutional level. With a focus on these elements, the authors feel core facilities will be well positioned to support scientific discovery in the future.
Language	English
Publishing date	2024-05-03
Publishing country	England
Document type	Journal Article
ZDB-ID	219263-9
ISSN	1365-2818 ; 0022-2720
ISSN (online)	1365-2818
ISSN	0022-2720
DOI	10.1111/jmi.13314
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Article ; Online: Correlative light and volume electron microscopy (vCLEM): How community participation can advance developing technologies.

Guerin, Christopher J / Lippens, Saskia

Journal of microscopy

2021 Volume 284, Issue 2, Page(s) 97–102

Abstract: Correlative light and electron microscopy is a valuable tool to image samples across resolution scales and link data on structure and function. While studies using this technique have been available since the 1960s, recent developments have enabled ... ...

Abstract	Correlative light and electron microscopy is a valuable tool to image samples across resolution scales and link data on structure and function. While studies using this technique have been available since the 1960s, recent developments have enabled applying these workflows to large volumes of cells and tissues. Much of the development in this area has been facilitated through the collaborative efforts of microscopists and commercial companies to bring the methods, hardware and image processing technologies needed into laboratories and core imaging facilities. This is a prime example of how what was once a niche area can be brought into the mainstream of microscopy by the efforts of imaging pioneers who push the boundaries of possibility.
Language	English
Publishing date	2021-09-20
Publishing country	England
Document type	Journal Article
ZDB-ID	219263-9
ISSN	1365-2818 ; 0022-2720
ISSN (online)	1365-2818
ISSN	0022-2720
DOI	10.1111/jmi.13056
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Article: A Î²-Turn Motif in the Steroid Hormone Receptorâs Ligand-Binding Domains Interacts with the Peptidyl-prolyl Isomerase (PPIase) Catalytic Site of the Immunophilin FKBP52

Byrne, Cillian / Henen Morkos A / Belnou Mathilde / Cantrelle FrancÌ§ois-Xavier / Kamah Amina / Qi Haoling / Giustiniani Julien / Chambraud BeÌatrice / Baulieu Etienne-Emile / Lippens Guy / Landrieu Isabelle / Jacquot Yves

Biochemistry. 2016 Sept. 27, v. 55, no. 38

2016

Abstract: The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor Î± (hERÎ±) and using nuclear magnetic resonance, we show here that the short VÂ³â¶â´PGFÂ³â¶â· sequence, which is ... ...

Abstract	The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor Î± (hERÎ±) and using nuclear magnetic resonance, we show here that the short VÂ³â¶â´PGFÂ³â¶â· sequence, which is located within its ligand-binding domain and adopts a type II Î²-turn conformation in the protein, binds the peptidyl-prolyl isomerase (PPIase or rotamase) FK1 domain of FKBP52. Interestingly, this turn motif displays strong similarities with the FKBP52 FK1 domain-binding moiety of macrolide immunomodulators such as rapamycin and GPI-1046, an immunophilin ligand with neuroprotective characteristics. An increase in the hydrophobicity of the residue preceding the proline and cyclization of the VPGF peptide strengthen its recognition by the FK1 domain of FKBP52. Replacement of the Pro residue with a dimethylproline also enhances this interaction. Our study not only contributes to a better understanding of how the interaction between the FK1 domain of FKBP52 and steroid hormone receptors most likely works but also opens new avenues for the synthesis of FKBP52 FK1 peptide ligands appropriate for the control of hormone-dependent physiological mechanisms or of the functioning of the Tau protein. Indeed, it has been shown that FKBP52 is involved in the intraneuronal dynamics of the Tau protein.
Keywords	active sites ; crystal structure ; estrogen receptors ; humans ; hydrophobicity ; immunomodulators ; ligands ; macrolides ; nuclear magnetic resonance spectroscopy ; peptidylprolyl isomerase ; proline ; protein conformation ; steroid hormone receptors
Language	English
Dates of publication	2016-0927
Size	p. 5366-5376.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1108-3
ISSN	1520-4995 ; 0006-2960
ISSN (online)	1520-4995
ISSN	0006-2960
DOI	10.1021%2Facs.biochem.6b00506
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Beyond the Hype: (How) Are Work Regimes Associated with Job Burnout?

du Bois, Kristen / Sterkens, Philippe / Lippens, Louis / Baert, Stijn / Derous, Eva

International journal of environmental research and public health

2023 Volume 20, Issue 4

Abstract: Job burnout affects countless workers and constitutes a major issue in working life. Prevention strategies such as offering part-time options and shorter working weeks have been widely advocated to address this issue. However, the relationship between ... ...

Abstract	Job burnout affects countless workers and constitutes a major issue in working life. Prevention strategies such as offering part-time options and shorter working weeks have been widely advocated to address this issue. However, the relationship between shorter work regimes and burnout risk has not yet been investigated across diverse working populations applying validated measures and frameworks for job burnout. Building on the most recent operationalisation of job burnout and the seminal job demands-resources theory, the purpose of the current study is to investigate whether shorter work regimes are associated with lower burnout risk and whether the job demands-resources explain this association. To this end, a heterogenous sample of 1006 employees representative for age and gender completed the Burnout Assessment Tool (BAT) and Workplace Stressors Assessment Questionnaire (WSAQ). Our mediation analyses yield a very small but significant indirect association between work regimes and burnout risk through job demands, but no significant total or direct association between work regimes and burnout risk. Our result suggests that employees in shorter work regimes experience slightly fewer job demands, but are equally prone to developing burnout as their full-time counterparts. The latter finding raises concerns about the sustainability of burnout prevention that focuses on mere work regimes instead of the root causes of burnout.
MeSH term(s)	Humans ; Job Satisfaction ; Burnout, Professional/etiology ; Workplace ; Employment ; Surveys and Questionnaires
Language	English
Publishing date	2023-02-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20043331
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Article: Resin comparison for serial block face scanning volume electron microscopy.

Borghgraef, Peter / Kremer, Anna / De Bruyne, Michiel / Guérin, Christopher J / Lippens, Saskia

Methods in cell biology

2023 Volume 177, Page(s) 33–54

Abstract: Serial Block Face Scanning Electron Microscopy (SBF-SEM) is one of several volume electron microscopy (vEM) techniques whose purpose is to reveal the nanostructure of cells and tissues in three dimensions. As one of the earliest, and possibly most widely ...

Abstract	Serial Block Face Scanning Electron Microscopy (SBF-SEM) is one of several volume electron microscopy (vEM) techniques whose purpose is to reveal the nanostructure of cells and tissues in three dimensions. As one of the earliest, and possibly most widely adopted of the disruptive vEM techniques there have been hundreds of publications using the method, although very few comparative studies of specimen preparation parameters. While some studies have focused on staining and specimen acquisition no comparison of resin embedding has yet been conducted. To this end we have surveyed the SBF-SEM literature to determine which resins are commonly used and compared them in both cellular and fixed tissue samples in an attempt to optimize sample preparation for: effectiveness of resin infiltration, resistance to charging and beam damage and clarity of image in the resulting data set. Here we present the results and discuss the various factors that go into optimizing specimen preparation for SBF-SEM.
MeSH term(s)	Microscopy, Electron, Scanning ; Imaging, Three-Dimensional/methods ; Volume Electron Microscopy ; Specimen Handling/methods
Language	English
Publishing date	2023-03-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	0091-679X
ISSN	0091-679X
DOI	10.1016/bs.mcb.2023.01.011
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Article: Copper(II) complexes of pentadentate 17-membered macrocyclic diamidodiamines with N, O or S as additional donors.

Herman, Gerrit G / Lippens, Werner / Goeminne A, Andr é M / Steenland, Mathieu / Blaton, Norbert M

Acta crystallographica. Section C, Crystal structure communications

2003 Volume 59, Issue Pt 7, Page(s) m294–8

Abstract: ... tetraazacycloheptadecanato)copper(II) trihydrate, [Cu(C(12)H(22)N(4)O(2)S)].3H(2)O, (III), are reported. The coordination ... groups in the basal plane. The apical position is occupied by an amine group, an ether O atom or a thio S ... atom. Trigonal distortion increases in the sequence S < O < N as apical donor. The relation between ...

Abstract	The crystal structures of (2,6-dioxo-1,4,7,11,14-pentaazacycloheptadecanato)copper(II) tetrahydrate, [Cu(C(12)H(23)N(5)O(2))].4H(2)O, (I), (3,16-dioxo-1-oxa-4,8,11,15-tetraazacycloheptadecanato)copper(II) pentahydrate, [Cu(C(12)H(22)N(4)O(3))].5H(2)O, (II), and (3,16-dioxo-1-thia-4,8,11,15-tetraazacycloheptadecanato)copper(II) trihydrate, [Cu(C(12)H(22)N(4)O(2)S)].3H(2)O, (III), are reported. The coordination geometry in each case is approximately square pyramidal with two amine groups and two deprotonated amide groups in the basal plane. The apical position is occupied by an amine group, an ether O atom or a thio S atom. Trigonal distortion increases in the sequence S < O < N as apical donor. The relation between the distortion in the basal plane of the complexes and the maxima in their electronic spectra is discussed.
MeSH term(s)	Copper/chemistry ; Crystallography, X-Ray ; Diamines/chemistry ; Hydrocarbons, Cyclic/chemistry ; Hydrogen Bonding ; Molecular Structure ; Nitrogen/chemistry ; Organometallic Compounds/chemistry ; Oxygen/chemistry ; Sulfur/chemistry
Chemical Substances	Diamines ; Hydrocarbons, Cyclic ; Organometallic Compounds ; Sulfur (70FD1KFU70) ; Copper (789U1901C5) ; Nitrogen (N762921K75) ; Oxygen (S88TT14065)
Language	English
Publishing date	2003-06-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	2025703-X
ISSN	2053-2296 ; 1600-5759 ; 0108-2701
ISSN (online)	2053-2296 ; 1600-5759
ISSN	0108-2701
DOI	10.1107/s0108270102023247
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Book ; Article ; Online: An Arab, an Asian, and a Black Guy Walk into a Job Interview

Van Borm, Hannah / Lippens, Louis / Baert, Stijn

Ethnic Stigma in Hiring after Controlling for Social Class

2022

Abstract: Over the last decades, researchers have found compelling evidence of hiring discrimination toward ethnic minorities based on field experiments using fictitious job applications. Despite increasing efforts to discover why ethnic minorities experience ... ...

Abstract	Over the last decades, researchers have found compelling evidence of hiring discrimination toward ethnic minorities based on field experiments using fictitious job applications. Despite increasing efforts to discover why ethnic minorities experience hiring penalties, the academic world has not yet found a satisfying answer. With this study, we aim to close this gap in the literature by conducting a state-of-the-art scenario experiment with genuine American recruiters. In the experiment, we ask recruiters to assess fictitious job applicants of various race-ethnicities but consistent social class. The applicants are rated on 22 statements related to the dominant explanations for ethnic discrimination in hiring that the models of taste-based and statistical discrimination have offered. We find that different race-ethnicity groups are evaluated rather similarly, except for Asian Americans, who are perceived to have better intellectual abilities and organizational skills and to be more ambitious, motivated, efficient, and open. These results suggest that the hiring discrimination found in previous experimental research might be overestimated because part of the reported hiring penalty may be attributed to aspects other than race-ethnicity.
Keywords	ddc:330 ; J71 ; J15 ; J24 ; hiring ; ethnic discrimination ; statistical discrimination ; social class ; stigma
Subject code	390
Language	English
Publisher	Bonn: Institute of Labor Economics (IZA)
Publishing country	de
Document type	Book ; Article ; Online
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Article ; Online: Mycobacterium tuberculosis retains viability in RNAlater buffer but not in GTC-TCEP and DNA/RNA Shield.

Krausser, L / Braet, S M / Benaamar, Z / Van Dyck-Lippens, M / de Jong, B C / Rigouts, L

Diagnostic microbiology and infectious disease

2023 Volume 106, Issue 1, Page(s) 115905

Abstract: Efficient inactivation of clinical samples containing mycobacteria is crucial for biosafety during shipment and handling. When stored in RNAlater, Mycobacterium tuberculosis H37Ra remains viable, and our results suggest that at -20 °C and 4 °C changes in ...

Abstract	Efficient inactivation of clinical samples containing mycobacteria is crucial for biosafety during shipment and handling. When stored in RNAlater, Mycobacterium tuberculosis H37Ra remains viable, and our results suggest that at -20 °C and 4 °C changes in the mycobacterial transcriptome are possible. Only GTC-TCEP and DNA/RNA Shield inactivate sufficiently for shipment.
MeSH term(s)	Humans ; Mycobacterium tuberculosis/genetics ; RNA ; DNA
Chemical Substances	RNA (63231-63-0) ; tris(2-carboxyethyl)phosphine (22OAC2MO2S) ; DNA (9007-49-2)
Language	English
Publishing date	2023-02-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	604920-5
ISSN	1879-0070 ; 0732-8893
ISSN (online)	1879-0070
ISSN	0732-8893
DOI	10.1016/j.diagmicrobio.2023.115905
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