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  1. Article: Computational and cellular exploration of the protein-protein interaction between

    Milton, Morgan E / Visick, Karen L

    Communicative & integrative biology

    2023  Volume 16, Issue 1, Page(s) 2203626

    Abstract: Anti-sigma factor antagonists SpoIIAA and RsbV ... ...

    Abstract Anti-sigma factor antagonists SpoIIAA and RsbV from
    Language English
    Publishing date 2023-04-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2451097-X
    ISSN 1942-0889
    ISSN 1942-0889
    DOI 10.1080/19420889.2023.2203626
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Vibrio fischeri

    Fung, Brittany L / Esin, Jeremy J / Visick, Karen L

    Journal of bacteriology

    2024  Volume 206, Issue 2, Page(s) e0037023

    Abstract: Multicellular communities of adherent bacteria known as biofilms are often detrimental in the context of a human host, making it important to study their formation and dispersal, especially in animal models. One such model is the symbiosis between the ... ...

    Abstract Multicellular communities of adherent bacteria known as biofilms are often detrimental in the context of a human host, making it important to study their formation and dispersal, especially in animal models. One such model is the symbiosis between the squid
    MeSH term(s) Animals ; Humans ; Aliivibrio fischeri/metabolism ; Biofilms ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Adhesins, Bacterial ; Decapodiformes/microbiology ; Symbiosis ; Polysaccharides
    Chemical Substances Bacterial Proteins ; Adhesins, Bacterial ; Polysaccharides
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00370-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Genetic Analysis Reveals a Requirement for the Hybrid Sensor Kinase RscS in

    Dial, Courtney N / Fung, Brittany L / Visick, Karen L

    Journal of bacteriology

    2023  Volume 205, Issue 7, Page(s) e0007523

    Abstract: The marine bacterium Vibrio fischeri initiates symbiotic colonization of its squid host, ...

    Abstract The marine bacterium Vibrio fischeri initiates symbiotic colonization of its squid host,
    MeSH term(s) Humans ; 4-Aminobenzoic Acid/metabolism ; Calcium/metabolism ; Aliivibrio fischeri/genetics ; Bacterial Proteins/genetics ; Biofilms ; Phosphotransferases/metabolism
    Chemical Substances 4-Aminobenzoic Acid (TL2TJE8QTX) ; Calcium (SY7Q814VUP) ; Bacterial Proteins ; Phosphotransferases (EC 2.7.-)
    Language English
    Publishing date 2023-06-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00075-23
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  4. Article ; Online: Computational and cellular exploration of the protein-protein interaction between Vibrio fischeri STAS domain protein SypA and serine kinase SypE

    Morgan E. Milton / Karen L. Visick

    Communicative & Integrative Biology, Vol 16, Iss

    2023  Volume 1

    Abstract: ABSTRACTAnti-sigma factor antagonists SpoIIAA and RsbV from Bacillus subtilis are the archetypes for single-domain STAS proteins in bacteria. The structures and mechanisms of these proteins along with their cognate anti-sigma factors have been well ... ...

    Abstract ABSTRACTAnti-sigma factor antagonists SpoIIAA and RsbV from Bacillus subtilis are the archetypes for single-domain STAS proteins in bacteria. The structures and mechanisms of these proteins along with their cognate anti-sigma factors have been well studied. SpoIIAA and RsbV utilize a partner-switching mechanism to regulate gene expression through protein-protein interactions to control the activity of their downstream anti-sigma factor partners. The Vibrio fischeri STAS domain protein SypA is also proposed to employ a partner-switching mechanism with its partner SypE, a serine kinase/phosphatase that controls SypA’s phosphorylation state. However, this regulation appears opposite to the canonical pathway, with SypA being the more downstream component rather than SypE. Here we explore the commonalities and differences between SypA and the canonical single-domain STAS proteins SpoIIAA and RsbV. We use a combination of AlphaFold 2 structure predictions and computational modeling to investigate the SypA-SypE binding interface. We then test a subset of our predictions in V.fischeri by generating and expressing SypA variants. Our findings suggest that, while SypA shares many sequence and structural traits with anti-sigma factor antagonist STAS domain proteins, there are significant differences that may account for SypA’s distinct regulatory output.
    Keywords Anti-sigma factor antagonists ; biofilm ; phosphorylation ; protein-protein interaction ; STAS ; Vibrio ; Biology (General) ; QH301-705.5
    Subject code 612
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher Taylor & Francis Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Vibrio fischeri: Laboratory Cultivation, Storage, and Common Phenotypic Assays.

    Christensen, David G / Visick, Karen L

    Current protocols in microbiology

    2020  Volume 57, Issue 1, Page(s) e103

    Abstract: Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of V. fischeri, as ... ...

    Abstract Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of V. fischeri, as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of V. fischeri. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Growth of V. fischeri from frozen stocks Basic Protocol 2: Growth of V. fischeri in rich, undefined liquid medium Alternate Protocol 1: Growth of V. fischeri in minimal medium Basic Protocol 3: Storage of V. fischeri in frozen stocks Basic Protocol 4: Biofilm assay on solid agar Alternate Protocol 2: Biofilm assay in shaking liquid culture Alternate Protocol 3: Biofilm assay in static liquid culture Basic Protocol 5: Motility assay Basic Protocol 6: Luminescence assay.
    MeSH term(s) Aliivibrio fischeri/drug effects ; Aliivibrio fischeri/genetics ; Aliivibrio fischeri/growth & development ; Aliivibrio fischeri/physiology ; Anti-Bacterial Agents/pharmacology ; Bacteriological Techniques/methods ; Biofilms ; Culture Media/chemistry ; Culture Media/metabolism ; Laboratories ; Phenotype ; Preservation, Biological/methods
    Chemical Substances Anti-Bacterial Agents ; Culture Media
    Language English
    Publishing date 2020-06-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2213675-7
    ISSN 1934-8533 ; 1934-8525
    ISSN (online) 1934-8533
    ISSN 1934-8525
    DOI 10.1002/cpmc.103
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  6. Article ; Online: High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization.

    Isenberg, Ruth Y / Christensen, David G / Visick, Karen L / Mandel, Mark J

    mBio

    2022  Volume 13, Issue 4, Page(s) e0167122

    Abstract: During colonization of the Hawaiian bobtail squid ( ...

    Abstract During colonization of the Hawaiian bobtail squid (
    MeSH term(s) Aliivibrio fischeri/physiology ; Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biofilms ; Cellulose/metabolism ; Cyclic GMP/metabolism ; Decapodiformes/microbiology ; Gene Expression Regulation, Bacterial ; Symbiosis
    Chemical Substances Bacterial Proteins ; Cellulose (9004-34-6) ; Cyclic GMP (H2D2X058MU)
    Language English
    Publishing date 2022-08-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.01671-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.

    Visick, Karen L / Stabb, Eric V / Ruby, Edward G

    Nature reviews. Microbiology

    2021  Volume 19, Issue 10, Page(s) 654–665

    Abstract: As our understanding of the human microbiome progresses, so does the need for natural experimental animal models that promote a mechanistic understanding of beneficial microorganism-host interactions. Years of research into the exclusive symbiosis ... ...

    Abstract As our understanding of the human microbiome progresses, so does the need for natural experimental animal models that promote a mechanistic understanding of beneficial microorganism-host interactions. Years of research into the exclusive symbiosis between the Hawaiian bobtail squid, Euprymna scolopes, and the bioluminescent bacterium Vibrio fischeri have permitted a detailed understanding of those bacterial genes underlying signal exchange and rhythmic activities that result in a persistent, beneficial association, as well as glimpses into the evolution of symbiotic competence. Migrating from the ambient seawater to regions deep inside the light-emitting organ of the squid, V. fischeri experiences, recognizes and adjusts to the changing environmental conditions. Here, we review key advances over the past 15 years that are deepening our understanding of these events.
    MeSH term(s) Aliivibrio fischeri/physiology ; Animals ; Decapodiformes/anatomy & histology ; Decapodiformes/microbiology ; Evolution, Molecular ; Hawaii ; Host Microbial Interactions ; Seawater/microbiology ; Symbiosis
    Language English
    Publishing date 2021-06-04
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 2139054-X
    ISSN 1740-1534 ; 1740-1526
    ISSN (online) 1740-1534
    ISSN 1740-1526
    DOI 10.1038/s41579-021-00557-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5865: Show issues Location:
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  8. Article ; Online: Genetic Manipulation of Vibrio fischeri.

    Christensen, David G / Tepavčević, Jovanka / Visick, Karen L

    Current protocols in microbiology

    2020  Volume 59, Issue 1, Page(s) e115

    Abstract: Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species. The bacterium has been used as a model organism to study symbiosis in the context of its association with its host, the Hawaiian bobtail squid Euprymna scolopes. The ... ...

    Abstract Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species. The bacterium has been used as a model organism to study symbiosis in the context of its association with its host, the Hawaiian bobtail squid Euprymna scolopes. The genetic tractability of this bacterium has facilitated the mapping of pathways that mediate interactions between these organisms. The protocols included here describe methods for genetic manipulation of V. fischeri. Following these protocols, the researcher will be able to introduce linear DNA via transformation to make chromosomal mutations, to introduce plasmid DNA via conjugation and subsequently eliminate unstable plasmids, to eliminate antibiotic resistance cassettes from the chromosome, and to randomly or specifically mutagenize V. fischeri with transposons. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Transformation of V. fischeri with linear DNA Basic Protocol 2: Plasmid transfer into V. fischeri via conjugation Support Protocol 1: Removing FRT-flanked antibiotic resistance cassettes from the V. fischeri genome Support Protocol 2: Eliminating unstable plasmids from V. fischeri Alternate Protocol 1: Introduction of exogenous DNA using a suicide plasmid Alternate Protocol 2: Site-specific transposon insertion using a suicide plasmid Alternate Protocol 3: Random transposon mutagenesis using a suicide plasmid.
    MeSH term(s) Aliivibrio fischeri/genetics ; Animals ; Conjugation, Genetic ; DNA, Fungal ; Decapodiformes/microbiology ; Genetic Techniques ; Plasmids ; Symbiosis ; Transformation, Genetic
    Chemical Substances DNA, Fungal
    Keywords covid19
    Language English
    Publishing date 2020-09-25
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2213675-7
    ISSN 1934-8533 ; 1934-8525
    ISSN (online) 1934-8533
    ISSN 1934-8525
    DOI 10.1002/cpmc.115
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  9. Article ; Online: Mutational Analysis of

    Shrestha, Prerana / Razvi, Ali / Fung, Brittany L / Eichinger, Steven J / Visick, Karen L

    Journal of bacteriology

    2022  Volume 204, Issue 7, Page(s) e0010922

    Abstract: The symbiont Vibrio fischeri uses motility to colonize its host. In numerous bacterial species, motility is negatively controlled by cyclic-di-GMP (c-di-GMP), which is produced by diguanylate cyclases (DGCs) with GGDEF domains and degraded by ... ...

    Abstract The symbiont Vibrio fischeri uses motility to colonize its host. In numerous bacterial species, motility is negatively controlled by cyclic-di-GMP (c-di-GMP), which is produced by diguanylate cyclases (DGCs) with GGDEF domains and degraded by phosphodiesterases with either EAL or HD-GYP domains. To begin to decode the functions of the 50 Vibrio fischeri genes with GGDEF, EAL, and/or HD-GYP domains, we deleted each gene and assessed each mutant's migration through tryptone broth salt (TBS) soft agar medium containing or lacking magnesium (Mg) and calcium (Ca), which are known to influence V. fischeri motility. We identified 6, 13, and 16 mutants with altered migration in TBS-Mg, TBS, and TBS-Ca soft agar, respectively, a result that underscores the importance of medium conditions in assessing gene function. A biosensor-based assay revealed that Mg and Ca affected c-di-GMP levels negatively and positively, respectively; the severe decrease in c-di-GMP caused by Mg addition correlates with its strong positive impact on bacterial migration. A mutant defective for
    MeSH term(s) Agar ; Aliivibrio fischeri/genetics ; Aliivibrio fischeri/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biofilms ; Calcium/metabolism ; Cyclic GMP/analogs & derivatives ; Cyclic GMP/metabolism ; Escherichia coli Proteins/metabolism ; Gene Expression Regulation, Bacterial ; Magnesium/metabolism ; Vibrio cholerae/metabolism
    Chemical Substances Bacterial Proteins ; Escherichia coli Proteins ; bis(3',5')-cyclic diguanylic acid (61093-23-0) ; Agar (9002-18-0) ; Cyclic GMP (H2D2X058MU) ; Magnesium (I38ZP9992A) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2022-06-27
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00109-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: The biofilm community resurfaces: new findings and post-pandemic progress.

    Greenwich, Jennifer L / Fleming, Derek / Banin, Ehud / Häussler, Susanne / Kjellerup, Birthe V / Sauer, Karin / Visick, Karen L / Fuqua, Clay

    Journal of bacteriology

    2023  Volume 205, Issue 10, Page(s) e0016623

    Abstract: The ninth American Society for Microbiology Conference on Biofilms was convened in-person on 13-17 November 2022 in Charlotte, NC. As the first of these conferences since prior to the start of the COVID-19 pandemic, the energy among the participants of ... ...

    Abstract The ninth American Society for Microbiology Conference on Biofilms was convened in-person on 13-17 November 2022 in Charlotte, NC. As the first of these conferences since prior to the start of the COVID-19 pandemic, the energy among the participants of the conference was clear, and the meeting was a tremendous success. The mixture of >330 oral and poster presentations resoundingly embodied the vitality of biofilm research across a wide range of topics and multiple scientific disciplines. Special activities, including a pre-conference symposium for early career researchers, further enhanced the attendee experience. As a general theme, the conference was deliberately structured to provide high levels of participation and engagement among early career scientists.
    MeSH term(s) Humans ; United States ; Pandemics ; Societies, Scientific ; Biofilms
    Language English
    Publishing date 2023-09-27
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00166-23
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