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  1. Article ; Online: Mycobacterial FtsEX-RipC interaction is required for normal growth and cell morphology in rifampicin and low ionic strength conditions.

    Samuels, Veneshley / Mulelu, Andani E / Ndlovu, Hlumani / Marakalala, Mohlopheni J

    Microbiology spectrum

    2024  Volume 12, Issue 3, Page(s) e0251523

    Abstract: Tuberculosis, a lung disease caused by : Importance: Mycobacterial cell growth and division are coordinated with regulated peptidoglycan hydrolysis. Understanding cell wall gene complexes that govern normal cell division and elongation will aid in the ...

    Abstract Tuberculosis, a lung disease caused by
    Importance: Mycobacterial cell growth and division are coordinated with regulated peptidoglycan hydrolysis. Understanding cell wall gene complexes that govern normal cell division and elongation will aid in the development of tools to disarm the ability of mycobacteria to survive immune-like and antibiotic stresses. We combined genetic analyses and scanning electron microscopy to analyze morphological changes of mycobacterial FtsEX and RipC mutants in stressful conditions. We demonstrate that FtsE, FtsX, FtsEX, and RipC are conditionally required for the survival of
    MeSH term(s) Cell Cycle Proteins/metabolism ; Bacterial Proteins/metabolism ; Rifampin/pharmacology ; Peptidoglycan/metabolism ; Mycobacterium smegmatis/genetics ; Mycobacterium smegmatis/metabolism ; Osmolar Concentration ; Anti-Bacterial Agents
    Chemical Substances Cell Cycle Proteins ; Bacterial Proteins ; Rifampin (VJT6J7R4TR) ; Peptidoglycan ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-01-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.02515-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: In vitro

    Marakalala, Mohlopheni J

    Bio-protocol

    2017  Volume 4, Issue 9

    Abstract: Macrophage recognition ... ...

    Abstract Macrophage recognition of
    Language English
    Publishing date 2017-12-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.1123
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Validation of proteins associated with pathological damage in human tuberculosis granulomas: study protocol.

    Mpotje, Thabo / More, Jessica / Rajkumar-Bhugeloo, Kerishka / Moodley, Denelle / Marakalala, Mohlopheni J

    Wellcome open research

    2023  Volume 8, Page(s) 139

    Abstract: The presence of the Tuberculosis (TB) disease-causing pathogen, ...

    Abstract The presence of the Tuberculosis (TB) disease-causing pathogen,
    Language English
    Publishing date 2023-03-28
    Publishing country England
    Document type Journal Article
    ISSN 2398-502X
    ISSN 2398-502X
    DOI 10.12688/wellcomeopenres.19226.1
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  4. Article: Tuberculosis: Role of Nuclear Medicine and Molecular Imaging With Potential Impact of Neutrophil-Specific Tracers.

    More, Stuart / Marakalala, Mohlopheni J / Sathekge, Michael

    Frontiers in medicine

    2021  Volume 8, Page(s) 758636

    Abstract: With Tuberculosis (TB) affecting millions of people worldwide, novel imaging modalities and tools, particularly nuclear medicine and molecular imaging, have grown with greater interest to assess the biology of the tuberculous granuloma and evolution ... ...

    Abstract With Tuberculosis (TB) affecting millions of people worldwide, novel imaging modalities and tools, particularly nuclear medicine and molecular imaging, have grown with greater interest to assess the biology of the tuberculous granuloma and evolution thereof. Much early work has been performed at the pre-clinical level using gamma single photon emission computed tomography (SPECT) agents exploiting certain characteristics of
    Language English
    Publishing date 2021-12-10
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2021.758636
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  5. Article ; Online: Validation of proteins associated with pathological damage in human tuberculosis granulomas

    Jessica More / Denelle Moodley / Mohlopheni J Marakalala / Kerishka Rajkumar-Bhugeloo / Thabo Mpotje

    Wellcome Open Research, Vol

    study protocol [version 1; peer review: 2 approved]

    2023  Volume 8

    Abstract: The presence of the Tuberculosis (TB) disease-causing pathogen, Mycobacterium Tuberculosis (Mtb), induces the development of a pathological feature termed granuloma, which the host uses to contain the bacteria. However, the granuloma may dissociate ... ...

    Abstract The presence of the Tuberculosis (TB) disease-causing pathogen, Mycobacterium Tuberculosis (Mtb), induces the development of a pathological feature termed granuloma, which the host uses to contain the bacteria. However, the granuloma may dissociate resulting in detrimental caseation of the lung. The disease contributes to a growing global burden of lung function challenges, warranting for more understanding of the TB-induced immunopathology. The current study aims to explore in detail host factors that drive pathological features of TB contributing to extensive lung tissue destruction. Lung tissue sections obtained from patients undergoing surgical resection will be processed and analyzed using histopathological assays including Immunohistochemistry, Immunofluorescence, Hematoxylin and Eosin staining and Laser Capture Microdissection. The findings will provide key host factors that associate with exacerbated lung immunopathology during TB.
    Keywords Mycobacterium tuberculosis ; TB Granulomas ; Host-Directed Therapies ; Inflammation ; Lung pathology ; eng ; Medicine ; R ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Wellcome
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Tuberculosis

    Stuart More / Mohlopheni J. Marakalala / Michael Sathekge

    Frontiers in Medicine, Vol

    Role of Nuclear Medicine and Molecular Imaging With Potential Impact of Neutrophil-Specific Tracers

    2021  Volume 8

    Abstract: With Tuberculosis (TB) affecting millions of people worldwide, novel imaging modalities and tools, particularly nuclear medicine and molecular imaging, have grown with greater interest to assess the biology of the tuberculous granuloma and evolution ... ...

    Abstract With Tuberculosis (TB) affecting millions of people worldwide, novel imaging modalities and tools, particularly nuclear medicine and molecular imaging, have grown with greater interest to assess the biology of the tuberculous granuloma and evolution thereof. Much early work has been performed at the pre-clinical level using gamma single photon emission computed tomography (SPECT) agents exploiting certain characteristics of Mycobacterium tuberculosis (MTb). Both antituberculous SPECT and positron emission tomography (PET) agents have been utilised to characterise MTb. Other PET tracers have been utilised to help to characterise the biology of MTb (including Gallium-68-labelled radiopharmaceuticals). Of all the tracers, 2-[18F]FDG has been studied extensively over the last two decades in many aspects of the treatment paradigm of TB: at diagnosis, staging, response assessment, restaging, and in potentially predicting the outcome of patients with latent TB infection. Its lower specificity in being able to distinguish different inflammatory cell types in the granuloma has garnered interest in reviewing more specific agents that can portend prognostic implications in the management of MTb. With the neutrophil being a cell type that portends this poorer prognosis, imaging this cell type may be able to answer more accurately questions relating to the tuberculous granuloma transmissivity and may help in characterising patients who may be at risk of developing active TB. The formyl peptide receptor 1(FPR1) expressed by neutrophils is a key marker in this process and is a potential target to characterise these areas. The pre-clinical work regarding the role of radiolabelled N-cinnamoyl –F-(D) L – F – (D) –L F (cFLFLF) (which is an antagonist for FPR1) using Technetium 99m-labelled conjugates and more recently radiolabelled with Gallium-68 and Copper 64 is discussed. It is the hope that further work with this tracer may accelerate its potential to be utilised in responding to many of the current diagnostic dilemmas and ...
    Keywords tuberculosis ; formyl peptide receptor ; Gallium-68 ; cFLFLF ; nuclear medicine ; molecular imaging ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Investigating neutrophil cell death in TB pathogenesis.

    Fisher, Kimone L / Rajkumar-Bhugeloo, Kerishka / Moodley, Denelle / Mpotje, Thabo / Ramsuran, Duran / Ndung'u, Thumbi / Marakalala, Mohlopheni J

    Gates open research

    2022  Volume 5, Page(s) 175

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2022-04-29
    Publishing country United States
    Document type Journal Article
    ISSN 2572-4754
    ISSN (online) 2572-4754
    DOI 10.12688/gatesopenres.13472.2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Signaling C-type lectin receptors in antimycobacterial immunity.

    Marakalala, Mohlopheni J / Ndlovu, Hlumani

    PLoS pathogens

    2017  Volume 13, Issue 6, Page(s) e1006333

    MeSH term(s) Animals ; Humans ; Immunity, Innate/immunology ; Lectins, C-Type/immunology ; Mycobacterium/immunology
    Chemical Substances Lectins, C-Type
    Language English
    Publishing date 2017-06-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1006333
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  9. Article ; Online: Signaling C-type lectin receptors in antimycobacterial immunity.

    Mohlopheni J Marakalala / Hlumani Ndlovu

    PLoS Pathogens, Vol 13, Iss 6, p e

    2017  Volume 1006333

    Keywords Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2017-06-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Acetic Acid Enables Molecular Enumeration of Mycobacterium tuberculosis from Sputum and Eliminates the Need for a Biosafety Level 3 Laboratory.

    Palekyte, Ana / Morkowska, Anna / Billington, Owen / Morris-Jones, Stephen / Millard, James / Marakalala, Mohlopheni J / Owolabi, Olumuyiwa / Sambou, Basil / Zumla, Alimuddin / Sutherland, Jayne S / McHugh, Timothy D / Honeyborne, Isobella

    Clinical chemistry

    2024  Volume 70, Issue 4, Page(s) 642–652

    Abstract: Background: Improved monitoring of Mycobacterium tuberculosis response to treatment is urgently required. We previously developed the molecular bacterial load assay (MBLA), but it is challenging to integrate into the clinical diagnostic laboratory due ... ...

    Abstract Background: Improved monitoring of Mycobacterium tuberculosis response to treatment is urgently required. We previously developed the molecular bacterial load assay (MBLA), but it is challenging to integrate into the clinical diagnostic laboratory due to a labor-intensive protocol required at biosafety level 3 (BSL-3). A modified assay was needed.
    Methods: The rapid enumeration and diagnostic for tuberculosis (READ-TB) assay was developed. Acetic acid was tested and compared to 4 M guanidine thiocyanate to be simultaneously bactericidal and preserve mycobacterial RNA. The extraction was based on silica column technology and incorporated low-cost reagents: 3 M sodium acetate and ethanol for the RNA extraction to replace phenol-chloroform. READ-TB was fully validated and compared directly to the MBLA using sputa collected from individuals with tuberculosis.
    Results: Acetic acid was bactericidal to M. tuberculosis with no significant loss in 16S rRNA or an unprotected mRNA fragment when sputum was stored in acetic acid at 25°C for 2 weeks or -20°C for 1 year. This novel use of acetic acid allows processing of sputum for READ-TB at biosafety level 2 (BSL-2) on sample receipt. READ-TB is semiautomated and rapid. READ-TB correlated with the MBLA when 85 human sputum samples were directly compared (R2 = 0.74).
    Conclusions: READ-TB is an improved version of the MBLA and is available to be adopted by clinical microbiology laboratories as a tool for tuberculosis treatment monitoring. READ-TB will have a particular impact in low- and middle-income countries (LMICs) for laboratories with no BSL-3 laboratory and for clinical trials testing new combinations of anti-tuberculosis drugs.
    MeSH term(s) Humans ; Mycobacterium tuberculosis/genetics ; Acetic Acid ; Sputum ; Laboratories ; RNA, Ribosomal, 16S/genetics ; Containment of Biohazards ; Tuberculosis/diagnosis ; Tuberculosis/microbiology
    Chemical Substances Acetic Acid (Q40Q9N063P) ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-03-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80102-1
    ISSN 1530-8561 ; 0009-9147
    ISSN (online) 1530-8561
    ISSN 0009-9147
    DOI 10.1093/clinchem/hvae013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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