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  1. Article ; Online: Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation.

    Krug, Johannes / Perner, Birgit / Albertz, Carolin / Mörl, Hanna / Hopfenmüller, Vera L / Englert, Christoph

    eLife

    2023  Volume 12

    Abstract: Body pigmentation is a limitation ... ...

    Abstract Body pigmentation is a limitation for
    MeSH term(s) Animals ; Fundulidae ; Zebrafish/genetics ; Clustered Regularly Interspaced Short Palindromic Repeats ; Aging/genetics ; Longevity/physiology ; Mammals/genetics
    Language English
    Publishing date 2023-02-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.81549
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  2. Article ; Online: Polyanions in Coagulation and Thrombosis: Focus on Polyphosphate and Neutrophils Extracellular Traps.

    Rangaswamy, Chandini / Englert, Hanna / Deppermann, Carsten / Renné, Thomas

    Thrombosis and haemostasis

    2021  Volume 121, Issue 8, Page(s) 1021–1030

    Abstract: Neutrophil extracellular traps (NETs) and polyphosphates (polyP) have been recognized as procoagulant polyanions. This review summarizes the activities and regulation of the two procoagulant mediators and compares their functions. NETs are composed of ... ...

    Abstract Neutrophil extracellular traps (NETs) and polyphosphates (polyP) have been recognized as procoagulant polyanions. This review summarizes the activities and regulation of the two procoagulant mediators and compares their functions. NETs are composed of DNA which like polyP is built of phosphate units linked by high-energy phosphoanhydride bonds. Both NETs and polyP form insoluble particulate surfaces composed of a DNA/histone meshwork or Ca
    MeSH term(s) Animals ; Blood Coagulation/drug effects ; Extracellular Traps/drug effects ; Extracellular Traps/metabolism ; Fibrinolytic Agents/therapeutic use ; Humans ; Neutrophils/drug effects ; Neutrophils/metabolism ; Polyphosphates/antagonists & inhibitors ; Polyphosphates/blood ; Signal Transduction ; Thrombosis/blood ; Thrombosis/drug therapy
    Chemical Substances Fibrinolytic Agents ; Polyphosphates
    Language English
    Publishing date 2021-02-16
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 518294-3
    ISSN 2567-689X ; 0340-6245
    ISSN (online) 2567-689X
    ISSN 0340-6245
    DOI 10.1055/a-1336-0526
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  3. Article: The Wilms Tumor Gene

    Hopfenmüller, Vera L / Perner, Birgit / Reuter, Hanna / Bates, Thomas J D / Große, Andreas / Englert, Christoph

    Frontiers in cell and developmental biology

    2022  Volume 9, Page(s) 809962

    Abstract: The Wilms tumor suppressor ... ...

    Abstract The Wilms tumor suppressor gene
    Language English
    Publishing date 2022-01-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2021.809962
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  4. Article ; Online: Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation

    Johannes Krug / Birgit Perner / Carolin Albertz / Hanna Mörl / Vera L Hopfenmüller / Christoph Englert

    eLife, Vol

    2023  Volume 12

    Abstract: Body pigmentation is a limitation for in vivo imaging and thus for the performance of longitudinal studies in biomedicine. A possibility to circumvent this obstacle is the employment of pigmentation mutants, which are used in fish species like zebrafish ... ...

    Abstract Body pigmentation is a limitation for in vivo imaging and thus for the performance of longitudinal studies in biomedicine. A possibility to circumvent this obstacle is the employment of pigmentation mutants, which are used in fish species like zebrafish and medaka. To address the basis of aging, the short-lived African killifish Nothobranchius furzeri has recently been established as a model organism. Despite its short lifespan, N. furzeri shows typical signs of mammalian aging including telomere shortening, accumulation of senescent cells, and loss of regenerative capacity. Here, we report the generation of a transparent N. furzeri line by the simultaneous inactivation of three key loci responsible for pigmentation. We demonstrate that this stable line, named klara, can serve as a tool for different applications including behavioral experiments and the establishment of a senescence reporter by integration of a fluorophore into the cdkn1a (p21) locus and in vivo microscopy of the resulting line.
    Keywords killifish ; pigmentation ; transparency ; senescence ; mate choice ; Medicine ; R ; Science ; Q ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher eLife Sciences Publications Ltd
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: The contact system in liver injury.

    Rangaswamy, Chandini / Mailer, Reiner K / Englert, Hanna / Konrath, Sandra / Renné, Thomas

    Seminars in immunopathology

    2021  Volume 43, Issue 4, Page(s) 507–517

    Abstract: Coagulation is controlled by a delicate balance of prothrombotic and antithrombotic mechanisms, to prevent both excessive blood loss from injured vessels and pathologic thrombosis. The liver plays a pivotal role in hemostasis through the synthesis of ... ...

    Abstract Coagulation is controlled by a delicate balance of prothrombotic and antithrombotic mechanisms, to prevent both excessive blood loss from injured vessels and pathologic thrombosis. The liver plays a pivotal role in hemostasis through the synthesis of plasma coagulation factors and their inhibitors that, in addition to thrombosis and hemostasis, orchestrates an array of inflammatory responses. As a result, impaired liver function has been linked with both hypercoagulability and bleeding disorders due to a pathologic balance of pro- and anticoagulant plasma factors. At sites of vascular injury, thrombus propagation that finally may occlude the blood vessel depends on negatively charged biopolymers, such as polyphosphates and extracellular DNA, that provide a physiological surface for contact activation of coagulation factor XII (FXII). FXII initiates the contact system that drives both the intrinsic pathway of coagulation, and formation of the inflammatory mediator bradykinin by the kallikrein-kinin system. Moreover, FXII facilitates receptor-mediated signalling, thereby promoting mitogenic activities, angiogenesis, and neutrophil stimulation with implications for liver diseases. Here, we summarize current knowledge on the FXII-driven contact system in liver diseases and review therapeutic approaches to target its activities during impaired liver function.
    MeSH term(s) Blood Coagulation ; Factor XII/metabolism ; Humans ; Kallikrein-Kinin System ; Liver/metabolism ; Thrombosis/etiology
    Chemical Substances Factor XII (9001-30-3)
    Language English
    Publishing date 2021-06-14
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2316828-6
    ISSN 1863-2300 ; 1863-2297
    ISSN (online) 1863-2300
    ISSN 1863-2297
    DOI 10.1007/s00281-021-00876-7
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  6. Article ; Online: Aging Activates the Immune System and Alters the Regenerative Capacity in the Zebrafish Heart.

    Reuter, Hanna / Perner, Birgit / Wahl, Florian / Rohde, Luise / Koch, Philipp / Groth, Marco / Buder, Katrin / Englert, Christoph

    Cells

    2022  Volume 11, Issue 3

    Abstract: Age-associated organ failure and degenerative diseases have a major impact on human health. Cardiovascular dysfunction has an increasing prevalence with age and is one of the leading causes of death. In contrast to humans, zebrafish have extraordinary ... ...

    Abstract Age-associated organ failure and degenerative diseases have a major impact on human health. Cardiovascular dysfunction has an increasing prevalence with age and is one of the leading causes of death. In contrast to humans, zebrafish have extraordinary regeneration capacities of complex organs including the heart. In addition, zebrafish has recently become a model organism in research on aging. Here, we have compared the ventricular transcriptome as well as the regenerative capacity after cryoinjury of old and young zebrafish hearts. We identified the immune system as activated in old ventricles and found muscle organization to deteriorate upon aging. Our data show an accumulation of immune cells, mostly macrophages, in the old zebrafish ventricle. Those immune cells not only increased in numbers but also showed morphological and behavioral changes with age. Our data further suggest that the regenerative response to cardiac injury is generally impaired and much more variable in old fish. Collagen in the wound area was already significantly enriched in old fish at 7 days post injury. Taken together, these data indicate an 'inflammaging'-like process in the zebrafish heart and suggest a change in regenerative response in the old.
    MeSH term(s) Aging ; Animals ; Cell Proliferation ; Heart/physiology ; Macrophages ; Zebrafish/physiology
    Language English
    Publishing date 2022-01-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11030345
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  7. Article: Polyanions in Coagulation and Thrombosis: Focus on Polyphosphate and Neutrophils Extracellular Traps

    Rangaswamy, Chandini / Englert, Hanna / Deppermann, Carsten / Renné, Thomas

    Thrombosis and Haemostasis

    2020  Volume 121, Issue 08, Page(s) 1021–1030

    Abstract: Neutrophil extracellular traps (NETs) and polyphosphates (polyP) have been recognized as procoagulant polyanions. This review summarizes the activities and regulation of the two procoagulant mediators and compares their functions. NETs are composed of ... ...

    Abstract Neutrophil extracellular traps (NETs) and polyphosphates (polyP) have been recognized as procoagulant polyanions. This review summarizes the activities and regulation of the two procoagulant mediators and compares their functions. NETs are composed of DNA which like polyP is built of phosphate units linked by high-energy phosphoanhydride bonds. Both NETs and polyP form insoluble particulate surfaces composed of a DNA/histone meshwork or Ca 2+ -rich nanoparticles, respectively. These polyanionic molecules modulate coagulation involving an array of mechanisms and trigger thrombosis via activation of the factor XII-driven procoagulant and proinflammatory contact pathway. Here, we outline the current knowledge on NETs and polyP with respect to their procoagulant and prothrombotic nature, strategies for interference of their activities in circulation, as well as the crosstalk between these two molecules. A better understanding of the underlying, cellular mechanisms will shed light on the therapeutic potential of targeting NETs and polyP in coagulation and thrombosis.
    Keywords polyanions ; contact activation ; NETs ; polyP
    Language English
    Publishing date 2020-12-11
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 518294-3
    ISSN 2567-689X ; 0340-6245
    ISSN (online) 2567-689X
    ISSN 0340-6245
    DOI 10.1055/a-1336-0526
    Database Thieme publisher's database

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  8. Article ; Online: Aging Activates the Immune System and Alters the Regenerative Capacity in the Zebrafish Heart

    Hanna Reuter / Birgit Perner / Florian Wahl / Luise Rohde / Philipp Koch / Marco Groth / Katrin Buder / Christoph Englert

    Cells, Vol 11, Iss 345, p

    2022  Volume 345

    Abstract: Age-associated organ failure and degenerative diseases have a major impact on human health. Cardiovascular dysfunction has an increasing prevalence with age and is one of the leading causes of death. In contrast to humans, zebrafish have extraordinary ... ...

    Abstract Age-associated organ failure and degenerative diseases have a major impact on human health. Cardiovascular dysfunction has an increasing prevalence with age and is one of the leading causes of death. In contrast to humans, zebrafish have extraordinary regeneration capacities of complex organs including the heart. In addition, zebrafish has recently become a model organism in research on aging. Here, we have compared the ventricular transcriptome as well as the regenerative capacity after cryoinjury of old and young zebrafish hearts. We identified the immune system as activated in old ventricles and found muscle organization to deteriorate upon aging. Our data show an accumulation of immune cells, mostly macrophages, in the old zebrafish ventricle. Those immune cells not only increased in numbers but also showed morphological and behavioral changes with age. Our data further suggest that the regenerative response to cardiac injury is generally impaired and much more variable in old fish. Collagen in the wound area was already significantly enriched in old fish at 7 days post injury. Taken together, these data indicate an ‘inflammaging’-like process in the zebrafish heart and suggest a change in regenerative response in the old.
    Keywords aging ; zebrafish heart ; age-dependent regeneration ; immune cells ; cryoinjury ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: The Wilms Tumor Gene wt1a Contributes to Blood-Cerebrospinal Fluid Barrier Function in Zebrafish

    Vera L. Hopfenmüller / Birgit Perner / Hanna Reuter / Thomas J. D. Bates / Andreas Große / Christoph Englert

    Frontiers in Cell and Developmental Biology, Vol

    2022  Volume 9

    Abstract: The Wilms tumor suppressor gene Wt1 encodes a zinc finger transcription factor, which is highly conserved among vertebrates. It is a key regulator of urogenital development and homeostasis but also plays a role in other organs including the spleen and ... ...

    Abstract The Wilms tumor suppressor gene Wt1 encodes a zinc finger transcription factor, which is highly conserved among vertebrates. It is a key regulator of urogenital development and homeostasis but also plays a role in other organs including the spleen and the heart. More recently additional functions for Wt1 in the mammalian central nervous system have been described. In contrast to mammals, bony fish possess two paralogous Wt1 genes, namely wt1a and wt1b. By performing detailed in situ hybridization analyses during zebrafish development, we discovered new expression domains for wt1a in the dorsal hindbrain, the caudal medulla and the spinal cord. Marker analysis identified wt1a expressing cells of the dorsal hindbrain as ependymal cells of the choroid plexus in the myelencephalic ventricle. The choroid plexus acts as a blood-cerebrospinal fluid barrier and thus is crucial for brain homeostasis. By employing wt1a mutant larvae and a dye accumulation assay with fluorescent tracers we demonstrate that Wt1a is required for proper choroid plexus formation and function. Thus, Wt1a contributes to the barrier properties of the choroid plexus in zebrafish, revealing an unexpected role for Wt1 in the zebrafish brain.
    Keywords Wilms tumor protein ; zebrafish ; choroid plexus ; CRISPR/cas9 ; dye accumulation assay ; fluorescent tracer ; Biology (General) ; QH301-705.5
    Subject code 572
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
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  10. Article ; Online: A peptide from the staphylococcal protein Efb binds P-selectin and inhibits the interaction of platelets with leukocytes.

    Wallis, Stuart / Wolska, Nina / Englert, Hanna / Posner, Mareike / Upadhyay, Abhishek / Renné, Thomas / Eggleston, Ian / Bagby, Stefan / Pula, Giordano

    Journal of thrombosis and haemostasis : JTH

    2021  Volume 20, Issue 3, Page(s) 729–741

    Abstract: Aims: P-selectin is a key surface adhesion molecule for the interaction of platelets with leukocytes. We have shown previously that the N-terminal domain of Staphylococcus aureus extracellular fibrinogen-binding protein (Efb) binds to P-selectin and ... ...

    Abstract Aims: P-selectin is a key surface adhesion molecule for the interaction of platelets with leukocytes. We have shown previously that the N-terminal domain of Staphylococcus aureus extracellular fibrinogen-binding protein (Efb) binds to P-selectin and interferes with platelet-leukocyte aggregate formation. Here, we aimed to identify the minimal Efb motif required for binding platelets and to characterize its ability to interfering with the formation of platelet-leukocyte aggregates.
    Methods and results: Using a library of synthetic peptides, we mapped the platelet-binding site to a continuous 20 amino acid stretch. The peptide Efb
    Conclusions: We have identified Efb
    MeSH term(s) Blood Platelets/metabolism ; Fibrinogen/metabolism ; Humans ; Inflammation/metabolism ; Leukocytes/metabolism ; P-Selectin/metabolism ; Peptides/metabolism ; Platelet Activation ; Thrombosis/metabolism
    Chemical Substances P-Selectin ; Peptides ; Fibrinogen (9001-32-5)
    Language English
    Publishing date 2021-12-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2112661-6
    ISSN 1538-7836 ; 1538-7933
    ISSN (online) 1538-7836
    ISSN 1538-7933
    DOI 10.1111/jth.15613
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    Zs.MO 295: Show issues

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