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Book ; Thesis: Untersuchungen zum COPII-abhängigen anterograden Transport der GIcNAc-1-Phosphotransferase aus dem endoplasmatischen reticulum

Ahmadi, Parimah

Analysis of the COPII dependent anterograde transport of GIcNAc-1-Phosphotransferase from the endoplasmic reticulum

2013

Title variant	Analysis of the COPII dependent anterograde transport of GIcNAc-1-Phosphotransferase from the endoplasmic reticulum
Author's details	von Parimah Ahmadi
Language	German
Size	57 Bl., Ill., graph. Darst., Tab
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., FB Biol. Bachelorarbeit--Hamburg, 2013
Note	nur Zsfassung auch in engl. Sprache
Accompanying material	1 CD-ROM
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Two simple and inexpensive methods for preparing DNA suitable for digital PCR from a small number of cells in 96-well plates.

Zou, Ziang / Guo, Linna / Ahmadi, Parimah / Hartjen, Philip / Gosau, Martin / Smeets, Ralf / Kluwe, Lan

Journal of clinical laboratory analysis

2020 Volume 35, Issue 1, Page(s) e23513

Abstract: Background: Although DNA of high quality can be easily prepared from cultured cells with commercially available kits, many studies involve a large number of samples which increases the cost drastically. We optimized two simple and inexpensive methods ... ...

Abstract	Background: Although DNA of high quality can be easily prepared from cultured cells with commercially available kits, many studies involve a large number of samples which increases the cost drastically. We optimized two simple and inexpensive methods for preparing DNA suitable for digital PCR from a small number of cells directly from wells of 96-well plates. Methods: Cells (number: 10 Results: For 1000 cells from one primary culture and two tumor cell lines, DNA was reproducible and obtained with recovery rate (obtained/expected amount of DNA) in the range of 50%-90% as measured by the fluorometer dyes instrument Qubit. Using 8 out of a total of 10 µL DNA solution for 1000 cells, both conventional PCR and digital PCR were successful. For digital PCR, more than 1600 positive droplets were obtained for DNA from 1000 cells using the Direct PCR Conclusions: Two methods are efficient, especially the Direct PCR
MeSH term(s)	Cell Line, Tumor ; Cells, Cultured ; DNA/analysis ; DNA/genetics ; DNA/isolation & purification ; Humans ; Polymerase Chain Reaction/methods ; Polymerase Chain Reaction/standards
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2020-08-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	645095-7
ISSN	1098-2825 ; 0887-8013
ISSN (online)	1098-2825
ISSN	0887-8013
DOI	10.1002/jcla.23513
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Article ; Online: Human dental pulp cells modulate CD8

Ahmadi, Parimah / Yan, Ming / Bauche, Andreas / Smeets, Ralf / Müller, Christa E / Koch-Nolte, Friedrich / Haag, Friedrich / Fliegert, Ralf / Kluwe, Lan / Schulze Zur Wiesch, Julian / Hartjen, Philip

Cellular immunology

2022 Volume 380, Page(s) 104589

Abstract: The pulp of human teeth contains a population of self-renewing stem cells that can regulate the functions of immune cells. When applied to patients, these cells can protect tissues from damage by excessive inflammation. We confirm that dental pulp cells ... ...

Abstract	The pulp of human teeth contains a population of self-renewing stem cells that can regulate the functions of immune cells. When applied to patients, these cells can protect tissues from damage by excessive inflammation. We confirm that dental pulp cells effectively inhibit the proliferation and activation of cytotoxic T cells in vitro, and show that they carry high levels of CD73, a key enzyme in the conversion of pro-inflammatory extracellular ATP to immunosuppressive adenosine. Given their accessibility and abundance, as well as their potential for allogeneic administration, dental pulp cells provide a valuable source for immunomodulatory therapy.
MeSH term(s)	5'-Nucleotidase/metabolism ; Adenosine/metabolism ; Adenosine Triphosphate/metabolism ; CD8-Positive T-Lymphocytes/metabolism ; Cell Proliferation ; Dental Pulp ; Humans
Chemical Substances	Adenosine Triphosphate (8L70Q75FXE) ; 5'-Nucleotidase (EC 3.1.3.5) ; Adenosine (K72T3FS567)
Language	English
Publishing date	2022-08-13
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80094-6
ISSN	1090-2163 ; 0008-8749
ISSN (online)	1090-2163
ISSN	0008-8749
DOI	10.1016/j.cellimm.2022.104589
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Article ; Online: Toward Tailoring the Degradation Rate of Magnesium-Based Biomaterials for Various Medical Applications: Assessing Corrosion, Cytocompatibility and Immunological Effects.

Hartjen, Philip / Wegner, Nils / Ahmadi, Parimah / Matthies, Levi / Nada, Ola / Fuest, Sandra / Yan, Ming / Knipfer, Christian / Gosau, Martin / Walther, Frank / Smeets, Ralf

International journal of molecular sciences

2021 Volume 22, Issue 2

Abstract: Magnesium (Mg)-based biomaterials hold considerable promise for applications in regenerative medicine. However, the degradation of Mg needs to be reduced to control toxicity caused by its rapid natural corrosion. In the process of developing new Mg ... ...

Abstract	Magnesium (Mg)-based biomaterials hold considerable promise for applications in regenerative medicine. However, the degradation of Mg needs to be reduced to control toxicity caused by its rapid natural corrosion. In the process of developing new Mg alloys with various surface modifications, an efficient assessment of the relevant properties is essential. In the present study, a WE43 Mg alloy with a plasma electrolytic oxidation (PEO)-generated surface was investigated. Surface microstructure, hydrogen gas evolution in immersion tests and cytocompatibility were assessed. In addition, a novel in vitro immunological test using primary human lymphocytes was introduced. On PEO-treated WE43, a larger number of pores and microcracks, as well as increased roughness, were observed compared to untreated WE43. Hydrogen gas evolution after two weeks was reduced by 40.7% through PEO treatment, indicating a significantly reduced corrosion rate. In contrast to untreated WE43, PEO-treated WE43 exhibited excellent cytocompatibility. After incubation for three days, untreated WE43 killed over 90% of lymphocytes while more than 80% of the cells were still vital after incubation with the PEO-treated WE43. PEO-treated WE43 slightly stimulated the activation, proliferation and toxin (perforin and granzyme B) expression of CD8
MeSH term(s)	Animals ; Cells, Cultured ; Coated Materials, Biocompatible/chemistry ; Coated Materials, Biocompatible/pharmacokinetics ; Coated Materials, Biocompatible/pharmacology ; Corrosion ; Equipment and Supplies ; Humans ; Immune System/drug effects ; Lymphocytes/drug effects ; Lymphocytes/physiology ; Magnesium/chemistry ; Magnesium/pharmacokinetics ; Magnesium/pharmacology ; Magnesium Compounds/chemistry ; Magnesium Compounds/pharmacokinetics ; Magnesium Compounds/pharmacology ; Materials Testing/methods ; Mice ; Oxidation-Reduction
Chemical Substances	Coated Materials, Biocompatible ; Magnesium Compounds ; Magnesium (I38ZP9992A)
Language	English
Publishing date	2021-01-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22020971
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Toward Tailoring the Degradation Rate of Magnesium-Based Biomaterials for Various Medical Applications

Philip Hartjen / Nils Wegner / Parimah Ahmadi / Levi Matthies / Ola Nada / Sandra Fuest / Ming Yan / Christian Knipfer / Martin Gosau / Frank Walther / Ralf Smeets

International Journal of Molecular Sciences, Vol 22, Iss 2, p

Assessing Corrosion, Cytocompatibility and Immunological Effects

2021 Volume 971

Abstract	Magnesium (Mg)-based biomaterials hold considerable promise for applications in regenerative medicine. However, the degradation of Mg needs to be reduced to control toxicity caused by its rapid natural corrosion. In the process of developing new Mg alloys with various surface modifications, an efficient assessment of the relevant properties is essential. In the present study, a WE43 Mg alloy with a plasma electrolytic oxidation (PEO)-generated surface was investigated. Surface microstructure, hydrogen gas evolution in immersion tests and cytocompatibility were assessed. In addition, a novel in vitro immunological test using primary human lymphocytes was introduced. On PEO-treated WE43, a larger number of pores and microcracks, as well as increased roughness, were observed compared to untreated WE43. Hydrogen gas evolution after two weeks was reduced by 40.7% through PEO treatment, indicating a significantly reduced corrosion rate. In contrast to untreated WE43, PEO-treated WE43 exhibited excellent cytocompatibility. After incubation for three days, untreated WE43 killed over 90% of lymphocytes while more than 80% of the cells were still vital after incubation with the PEO-treated WE43. PEO-treated WE43 slightly stimulated the activation, proliferation and toxin (perforin and granzyme B) expression of CD8 + T cells. This study demonstrates that the combined assessment of corrosion, cytocompatibility and immunological effects on primary human lymphocytes provide a comprehensive and effective procedure for characterizing Mg variants with tailorable degradation and other features. PEO-treated WE43 is a promising candidate for further development as a degradable biomaterial.
Keywords	magnesium ; plasma electrolytic oxidation (PEO) ; microstructure ; hydrogen evolution ; cytocompatibility ; immunological effects ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: B cell analysis in SARS-CoV-2 versus malaria: Increased frequencies of plasmablasts and atypical memory B cells in COVID-19.

Wildner, Nils H / Ahmadi, Parimah / Schulte, Sophia / Brauneck, Franziska / Kohsar, Matin / Lütgehetmann, Marc / Beisel, Claudia / Addo, Marylyn M / Haag, Friedrich / Schulze Zur Wiesch, Julian

Journal of leukocyte biology

2020 Volume 109, Issue 1, Page(s) 77–90

Abstract: B cells play a central role in antiviral and antiparasitic immunity, not only as producers of antibodies, but also as APCs and mediators of inflammation. In this study, we used 16-color flow cytometry analysis to investigate the frequency, ... ...

Abstract	B cells play a central role in antiviral and antiparasitic immunity, not only as producers of antibodies, but also as APCs and mediators of inflammation. In this study, we used 16-color flow cytometry analysis to investigate the frequency, differentiation, and activation status of peripheral B cells of patients with SARS-CoV-2 infection or acute Plasmodium falciparum malaria compared with the healthy individuals. As a main result, we observed an increase of the frequency of (CD27
MeSH term(s)	Adult ; Aged ; Antigens, CD/immunology ; COVID-19/immunology ; COVID-19/pathology ; Female ; Humans ; Immunologic Memory ; Malaria, Falciparum/immunology ; Malaria, Falciparum/pathology ; Male ; Middle Aged ; Plasma Cells/immunology ; Plasma Cells/pathology ; Plasmodium falciparum/immunology ; SARS-CoV-2/immunology
Chemical Substances	Antigens, CD
Language	English
Publishing date	2020-12-04
Publishing country	England
Document type	Clinical Trial ; Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1002/JLB.5COVA0620-370RR
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Zs.A 603: Show issues

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Article ; Online: Defining the CD39/CD73 Axis in SARS-CoV-2 Infection: The CD73

Ahmadi, Parimah / Hartjen, Philip / Kohsar, Matin / Kummer, Silke / Schmiedel, Stefan / Bockmann, Jan-Hendrik / Fathi, Anahita / Huber, Samuel / Haag, Friedrich / Schulze Zur Wiesch, Julian

Cells

2020 Volume 9, Issue 8

Abstract: The ectonucleotidases CD39 and CD73 regulate immune responses by balancing extracellular ATP and adenosine in inflammation and are likely to be involved in the pathophysiology of COVID-19. Here, we analyzed CD39 and CD73 on different lymphocyte ... ...

Abstract	The ectonucleotidases CD39 and CD73 regulate immune responses by balancing extracellular ATP and adenosine in inflammation and are likely to be involved in the pathophysiology of COVID-19. Here, we analyzed CD39 and CD73 on different lymphocyte populations in a small cohort of COVID-19 patients and in healthy individuals. We describe a significantly lower level of expression of CD73 on cytotoxic lymphocyte populations, including CD8
MeSH term(s)	5'-Nucleotidase/metabolism ; Adenosine/metabolism ; Adult ; Aged ; Apyrase/metabolism ; Betacoronavirus ; COVID-19 ; Coronavirus Infections/enzymology ; Coronavirus Infections/immunology ; Female ; GPI-Linked Proteins/metabolism ; Granzymes/metabolism ; Humans ; Inflammation/enzymology ; Inflammation/immunology ; Interferon-gamma/metabolism ; Killer Cells, Natural/immunology ; Male ; Middle Aged ; Natural Killer T-Cells/immunology ; Pandemics ; Perforin/metabolism ; Pneumonia, Viral/enzymology ; Pneumonia, Viral/immunology ; SARS-CoV-2 ; Signal Transduction/immunology ; T-Lymphocytes, Cytotoxic/immunology ; T-Lymphocytes, Cytotoxic/metabolism ; Tumor Necrosis Factor-alpha/metabolism
Chemical Substances	GPI-Linked Proteins ; Tumor Necrosis Factor-alpha ; Perforin (126465-35-8) ; Interferon-gamma (82115-62-6) ; 5'-Nucleotidase (EC 3.1.3.5) ; NT5E protein, human (EC 3.1.3.5) ; Granzymes (EC 3.4.21.-) ; Apyrase (EC 3.6.1.5) ; ENTPD1 protein, human (EC 3.6.1.5) ; Adenosine (K72T3FS567)
Keywords	covid19
Language	English
Publishing date	2020-07-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells9081750
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Defining the CD39/CD73 Axis in SARS-CoV-2 Infection

Parimah Ahmadi / Philip Hartjen / Matin Kohsar / Silke Kummer / Stefan Schmiedel / Jan-Hendrik Bockmann / Anahita Fathi / Samuel Huber / Friedrich Haag / Julian Schulze zur Wiesch

Cells, Vol 9, Iss 1750, p

The CD73 - Phenotype Identifies Polyfunctional Cytotoxic Lymphocytes

2020 Volume 1750

Abstract	The ectonucleotidases CD39 and CD73 regulate immune responses by balancing extracellular ATP and adenosine in inflammation and are likely to be involved in the pathophysiology of COVID-19. Here, we analyzed CD39 and CD73 on different lymphocyte populations in a small cohort of COVID-19 patients and in healthy individuals. We describe a significantly lower level of expression of CD73 on cytotoxic lymphocyte populations, including CD8 + T, natural killer T (NKT), and natural killer (NK) cells, during COVID-19. Interestingly, the decrease of CD73 on CD8 + T cells and NKT cells correlated with serum ferritin levels. Furthermore, we observed distinct functional differences between the CD73 + and CD73 - subsets of CD8 + T cells and NKT cells with regard to cytokine/toxin secretion. In COVID-19 patients, the majority of the CD73 - CD8 + T cells were capable of secreting granzyme B, perforin, tumor necrosis factor (TNF-α) or interferon-gamma (IFN-γ). To conclude, in this first study of CD39 and CD73 expression of lymphocytes in COVID-19, we show that CD8 + T cells and NKT cells lacking CD73 possess a significantly higher cytotoxic effector functionality compared to their CD73 + counterparts. Future studies should investigate differences of cellular CD39 and CD73 expression in patients at different disease stages and their potential as prognostic markers or targets for immunomodulatory therapies.
Keywords	COVID-19 ; purinergic signaling ; CD73 ; CD39 ; cytotoxic lymphocytes ; granzyme B ; Biology (General) ; QH301-705.5 ; covid19
Subject code	333
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Defining the CD39/CD73 Axis in SARS-CoV-2 Infection: The CD73- Phenotype Identifies Polyfunctional Cytotoxic Lymphocytes

Ahmadi, Parimah / Hartjen, Philip / Kohsar, Matin / Kummer, Silke / Schmiedel, Stefan / Bockmann, Jan-Hendrik / Fathi, Anahita / Huber, Samuel / Haag, Friedrich / Schulze Zur Wiesch, Julian

Abstract	The ectonucleotidases CD39 and CD73 regulate immune responses by balancing extracellular ATP and adenosine in inflammation and are likely to be involved in the pathophysiology of COVID-19. Here, we analyzed CD39 and CD73 on different lymphocyte populations in a small cohort of COVID-19 patients and in healthy individuals. We describe a significantly lower level of expression of CD73 on cytotoxic lymphocyte populations, including CD8+ T, natural killer T (NKT), and natural killer (NK) cells, during COVID-19. Interestingly, the decrease of CD73 on CD8+ T cells and NKT cells correlated with serum ferritin levels. Furthermore, we observed distinct functional differences between the CD73+ and CD73- subsets of CD8+ T cells and NKT cells with regard to cytokine/toxin secretion. In COVID-19 patients, the majority of the CD73-CD8+ T cells were capable of secreting granzyme B, perforin, tumor necrosis factor (TNF-α) or interferon-gamma (IFN-Î³). To conclude, in this first study of CD39 and CD73 expression of lymphocytes in COVID-19, we show that CD8+ T cells and NKT cells lacking CD73 possess a significantly higher cytotoxic effector functionality compared to their CD73+ counterparts. Future studies should investigate differences of cellular CD39 and CD73 expression in patients at different disease stages and their potential as prognostic markers or targets for immunomodulatory therapies.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #669617
Database	COVID19

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