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Article ; Online: Cell Cultures for Virology: Usability, Advantages, and Prospects.

Dolskiy, Alexander A / Grishchenko, Irina V / Yudkin, Dmitry V

International journal of molecular sciences

2020 Volume 21, Issue 21

Abstract: Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. ... ...

Abstract	Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines. These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.
MeSH term(s)	Animals ; Cell Culture Techniques/methods ; Genes, Reporter ; Genetic Engineering ; Humans ; Synthetic Biology/methods ; Virology/methods ; Viruses/growth & development
Language	English
Publishing date	2020-10-27
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21217978
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Reactivation of FMR1 gene expression is a promising strategy for fragile X syndrome therapy.

Shitik, Ekaterina M / Velmiskina, Anastasia A / Dolskiy, Alexander A / Yudkin, Dmitry V

Gene therapy

2020 Volume 27, Issue 6, Page(s) 247–253

Abstract: Fragile X syndrome (FXS) is the most common form of intellectual disability and autism spectrum disorder and is caused by CGG repeat expansion in the promoter region of the FMR1 gene, which encodes fragile X mental retardation protein. This event leads ... ...

Abstract	Fragile X syndrome (FXS) is the most common form of intellectual disability and autism spectrum disorder and is caused by CGG repeat expansion in the promoter region of the FMR1 gene, which encodes fragile X mental retardation protein. This event leads to gene silencing and the loss of gene products through DNA methylation and chromatin remodeling. Due to the pathogenesis of FXS, targeted, symptomatic, and etiological approaches have been developed for its treatment. Despite their rapid development, symptomatic and targeted treatment approaches have numerous limitations; etiological approaches have the greatest potential because they affect the main causes of transcriptional silencing. In this review, we consider three potential etiological therapeutic methods that affect the reactivation of FMR1 gene expression: treatment with inhibitors of chromatin-modifying enzymes, the use of noncoding RNAs and the application of gene therapy. Inhibitors of chromatin-modifying enzymes are not clinically applicable because of their low reactivity and high cytotoxicity, and noncoding RNAs are currently only under study. Thus, we discuss gene therapy as the most promising approach for treating FXS in the near future.
MeSH term(s)	Autism Spectrum Disorder ; DNA Methylation ; Fragile X Mental Retardation Protein/genetics ; Fragile X Syndrome/genetics ; Fragile X Syndrome/therapy ; Gene Expression ; Gene Silencing ; Humans
Chemical Substances	FMR1 protein, human ; Fragile X Mental Retardation Protein (139135-51-6)
Language	English
Publishing date	2020-03-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1191036-7
ISSN	1476-5462 ; 0969-7128
ISSN (online)	1476-5462
ISSN	0969-7128
DOI	10.1038/s41434-020-0141-0
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Article ; Online: Cell Cultures for Virology

Alexander A. Dolskiy / Irina V. Grishchenko / Dmitry V. Yudkin

International Journal of Molecular Sciences, Vol 21, Iss 7978, p

Usability, Advantages, and Prospects

2020 Volume 7978

Abstract	Virus detection in natural and clinical samples is a complicated problem in research and diagnostics. There are different approaches for virus isolation and identification, including PCR, CRISPR/Cas technology, NGS, immunoassays, and cell-based assays. Following the development of genetic engineering methods, approaches that utilize cell cultures have become useful and informative. Molecular biology methods allow increases in the sensitivity and specificity of cell cultures for certain viruses and can be used to generate reporter cell lines. These cell lines express specific reporter proteins (e.g., GFP, luciferase, and CAT) in response to virus infection that can be detected in a laboratory setting. The development of genome editing and synthetic biology methods has given rise to new perspectives regarding the design of virus reporter systems in cell cultures. This review is aimed at describing both virology methods in general and examples of the development of cell-based methods that exist today.
Keywords	cell cultures ; virus ; reporter construction ; virus-inducible expression ; cell-based method ; cell susceptibility ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	600 ; 616
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: miRNA expression and interaction with the 3'UTR of FMR1 in FRAXopathy pathogenesis.

Dolskiy, Alexander A / Yarushkin, Andrey A / Grishchenko, Irina V / Lemskaya, Natalya A / Pindyurin, Alexey V / Boldyreva, Lidiya V / Pustylnyak, Vladimir O / Yudkin, Dmitry V

Non-coding RNA research

2020 Volume 6, Issue 1, Page(s) 1–7

Abstract: FRAXopathies are caused by the expansion of the CGG repeat in the 5'UTR of ... ...

Abstract	FRAXopathies are caused by the expansion of the CGG repeat in the 5'UTR of the
Language	English
Publishing date	2020-12-03
Publishing country	Netherlands
Document type	Journal Article
ISSN	2468-0540
ISSN (online)	2468-0540
DOI	10.1016/j.ncrna.2020.11.006
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Article: A rare familial rearrangement of chromosomes 9 and 15 associated with intellectual disability: a clinical and molecular study.

Lemskaya, Natalya A / Romanenko, Svetlana A / Rezakova, Mariia A / Filimonova, Elena A / Prokopov, Dmitry Yu / Dolskiy, Alexander A / Perelman, Polina L / Maksimova, Yulia V / Shorina, Asia R / Yudkin, Dmitry V

Molecular cytogenetics

2021 Volume 14, Issue 1, Page(s) 47

Abstract: Background: There are many reports on rearrangements occurring separately in the regions of chromosomes 9p and 15q affected in the case under study. 15q duplication syndrome is caused by the presence of at least one extra maternally derived copy of the ... ...

Abstract	Background: There are many reports on rearrangements occurring separately in the regions of chromosomes 9p and 15q affected in the case under study. 15q duplication syndrome is caused by the presence of at least one extra maternally derived copy of the Prader-Willi/Angelman critical region. Trisomy 9p is the fourth most frequent chromosome anomaly with a clinically recognizable syndrome often accompanied by intellectual disability. Here we report a new case of a patient with maternally derived unique complex sSMC resulting in partial trisomy of both chromosomes 9 and 15 associated with intellectual disability. Case presentation: We characterise a supernumerary derivative chromosome 15: 47,XY,+der(15)t(9;15)(p21.2;q13.2), likely resulting from 3:1 malsegregation during maternal gametogenesis. Chromosomal analysis showed that a phenotypically normal mother is a carrier of balanced translocation t(9;15)(p21.1;q13.2). Her 7-year-old son showed signs of intellectual disability and a number of physical abnormalities including bilateral cryptorchidism and congenital megaureter. The child's magnetic resonance imaging showed changes in brain volume and in structural and functional connectivity revealing phenotypic changes caused by the presence of the extra chromosome material, whereas the mother's brain MRI was normal. Sequence analyses of the microdissected der(15) chromosome detected two breakpoint regions: HSA9:25,928,021-26,157,441 (9p21.2 band) and HSA15:30,552,104-30,765,905 (15q13.2 band). The breakpoint region on chromosome HSA9 is poor in genetic features with several areas of high homology with the breakpoint region on chromosome 15. The breakpoint region on HSA15 is located in the area of a large segmental duplication. Conclusions: We discuss the case of these phenotypic and brain MRI features in light of reported signatures for 9p partial trisomy and 15 duplication syndromes and analyze how the genomic characteristics of the found breakpoint regions have contributed to the origin of the derivative chromosome. We recommend MRI for all patients with a developmental delay, especially in cases with identified rearrangements, to accumulate more information on brain phenotypes related to chromosomal syndromes.
Language	English
Publishing date	2021-10-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2420849-8
ISSN	1755-8166
ISSN	1755-8166
DOI	10.1186/s13039-021-00565-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Inhibitors of Histone Deacetylases Are Weak Activators of the

Dolskiy, Alexander A / Pustylnyak, Vladimir O / Yarushkin, Andrey A / Lemskaya, Natalya A / Yudkin, Dmitry V

BioMed research international

2017 Volume 2017, Page(s) 3582601

Abstract: Fragile X syndrome is the most common cause of inherited intellectual disability in humans. It is a result of CGG repeat expansion in the 5' untranslated region (5' UTR) of ... ...

Abstract	Fragile X syndrome is the most common cause of inherited intellectual disability in humans. It is a result of CGG repeat expansion in the 5' untranslated region (5' UTR) of the
MeSH term(s)	Cell Line ; Cell Survival/drug effects ; Depsipeptides/pharmacology ; Fragile X Mental Retardation Protein/genetics ; Fragile X Syndrome/drug therapy ; Fragile X Syndrome/genetics ; Fragile X Syndrome/pathology ; Gene Expression Regulation/drug effects ; Heterochromatin/genetics ; Histone Deacetylase Inhibitors/administration & dosage ; Humans ; Hydroxamic Acids/pharmacology ; Male ; Promoter Regions, Genetic/genetics ; Trinucleotide Repeat Expansion/genetics
Chemical Substances	Depsipeptides ; FMR1 protein, human ; Heterochromatin ; Histone Deacetylase Inhibitors ; Hydroxamic Acids ; Fragile X Mental Retardation Protein (139135-51-6) ; vorinostat (58IFB293JI) ; romidepsin (CX3T89XQBK)
Language	English
Publishing date	2017
Publishing country	United States
Document type	Journal Article
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2017/3582601
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Article: Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line.

Dolskiy, Alexander A / Bodnev, Sergei A / Nazarenko, Anastasia A / Smirnova, Anastasia M / Pyankova, Olga G / Matveeva, Anna K / Grishchenko, Irina V / Tregubchak, Tatiana V / Pyankov, Oleg V / Ryzhikov, Alexander B / Gavrilova, Elena V / Maksyutov, Rinat A / Yudkin, Dmitry V

Frontiers in molecular biosciences

2020 Volume 7, Page(s) 616798

Abstract: SARS-CoV-2, which emerged in Wuhan (China), has become a great worldwide problem in 2020 and has led to more than 1,000,000 deaths worldwide. Many laboratories are searching for ways to fight this pandemic. We studied the action of the cellular antiviral ...

Abstract	SARS-CoV-2, which emerged in Wuhan (China), has become a great worldwide problem in 2020 and has led to more than 1,000,000 deaths worldwide. Many laboratories are searching for ways to fight this pandemic. We studied the action of the cellular antiviral protein tetherin, which is encoded by the BST2 gene. We deleted the transmembrane domain-encoding part of the gene in the Vero cell line. The transmembrane domain is a target for virus-antagonizing proteins. We showed a decrease in SARS-CoV-2 in cells with deleted transmembrane BST2 domains compared to the initial Vero cell line. Similar results were obtained for SARS-CoV and avian influenza virus. This finding may help the development of antiviral therapies competitively targeting the transmembrane domain of tetherin with viral-antagonizing proteins.
Language	English
Publishing date	2020-12-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2020.616798
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Article: The Tissue Distribution of SARS-CoV-2 in Transgenic Mice With Inducible Ubiquitous Expression of hACE2.

Dolskiy, Alexander A / Gudymo, Andrey S / Taranov, Oleg S / Grishchenko, Irina V / Shitik, Ekaterina M / Prokopov, Dmitry Yu / Soldatov, Vladislav O / Sobolevskaya, Elvira V / Bodnev, Sergey A / Danilchenko, Natalia V / Moiseeva, Anastasia A / Torzhkova, Polina Y / Bulanovich, Yulia A / Onhonova, Galina S / Ivleva, Elena K / Kubekina, Marina V / Belykh, Andrey E / Tregubchak, Tatiana V / Ryzhikov, Alexander B /

Gavrilova, Elena V / Maksyutov, Rinat A / Deykin, Alexey V / Yudkin, Dmitry V

Frontiers in molecular biosciences

2022 Volume 8, Page(s) 821506

Abstract: The novel coronavirus disease COVID-19 has become one of the most socially significant infections. One of the main models for COVID-19 pathogenesis study and anti-COVID-19 drug development is laboratory animals sensitive to the virus. Herein, we report ... ...

Abstract	The novel coronavirus disease COVID-19 has become one of the most socially significant infections. One of the main models for COVID-19 pathogenesis study and anti-COVID-19 drug development is laboratory animals sensitive to the virus. Herein, we report SARS-CoV-2 infection in novel transgenic mice conditionally expressing human ACE2 (hACE2), with a focus on viral distribution after intranasal inoculation. Transgenic mice carrying
Language	English
Publishing date	2022-01-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2021.821506
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Article ; Online: Robertsonian translocation 13/14 associated with rRNA genes overexpression and intellectual disability

Alexander A. Dolskiy / Natalya A. Lemskaya / Yulia V. Maksimova / Asia R. Shorina / Irina S. Kolesnikova / Dmitry V. Yudkin

Egyptian Journal of Medical Human Genetics, Vol 19, Iss 2, Pp 141-

2018 Volume 145

Abstract: Background: The Robertsonian translocations inherited from parents with a normal phenotype are often discovered through children with pathogenesis. The exact causes of pathologies in children with clinical manifestations are often unknown and vary ... ...

Abstract	Background: The Robertsonian translocations inherited from parents with a normal phenotype are often discovered through children with pathogenesis. The exact causes of pathologies in children with clinical manifestations are often unknown and vary greatly in the reported cases: uniparental disomy, de novo rearrangements, changes in methylation patterns and gene expression, including ribosomal genes. Aim of the study: Molecular-cytogenetic investigation of a clinical case of intellectual disability. Material and methods: GTG-banding, Ag-NOR staining, fluorescent in situ hybridization, PCR, real-time PCR. Results: We describe a family case of a translocation rob (13; 14) and elevated rRNA expression in the proband with developmental delay and in his phenotypically normal mother. We show the loss of the p-arms of original chromosomes and the absence of NORs on the derived chromosome. The whole-chromosome uniparental disomy is excluded. Conclusion: The translocated chromosome in the proband was most likely inherited from the mother and did not come about de novo with normal chromosomes 13 and 14 being obtained from the father. The cause of the pathogenesis in the proband still remains unknown. We hypothesize that it could be caused by impaired imprinting manifesting in altered methylation levels of loci on the derivative chromosome. Keywords: Robertsonian translocation, rRNA, Chromosome 13, Chromosome 14, Intellectual disability
Keywords	Medicine (General) ; R5-920 ; Genetics ; QH426-470
Language	English
Publishing date	2018-04-01T00:00:00Z
Publisher	SpringerOpen
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Deletion of BST2 Cytoplasmic and Transmembrane N-Terminal Domains Results in SARS-CoV, SARS-CoV-2, and Influenza Virus Production Suppression in a Vero Cell Line

Alexander A. Dolskiy / Sergei A. Bodnev / Anastasia A. Nazarenko / Anastasia M. Smirnova / Olga G. Pyankova / Anna K. Matveeva / Irina V. Grishchenko / Tatiana V. Tregubchak / Oleg V. Pyankov / Alexander B. Ryzhikov / Elena V. Gavrilova / Rinat A. Maksyutov / Dmitry V. Yudkin

Frontiers in Molecular Biosciences, Vol

2020 Volume 7

Abstract	SARS-CoV-2, which emerged in Wuhan (China), has become a great worldwide problem in 2020 and has led to more than 1,000,000 deaths worldwide. Many laboratories are searching for ways to fight this pandemic. We studied the action of the cellular antiviral protein tetherin, which is encoded by the BST2 gene. We deleted the transmembrane domain-encoding part of the gene in the Vero cell line. The transmembrane domain is a target for virus-antagonizing proteins. We showed a decrease in SARS-CoV-2 in cells with deleted transmembrane BST2 domains compared to the initial Vero cell line. Similar results were obtained for SARS-CoV and avian influenza virus. This finding may help the development of antiviral therapies competitively targeting the transmembrane domain of tetherin with viral-antagonizing proteins.
Keywords	SARS-CoV ; SARS-CoV-2 ; influenza ; BST2 ; tetherin ; Biology (General) ; QH301-705.5
Subject code	572
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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